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The map label for this gene is proP1 [H]

Identifier: 189184696

GI number: 189184696

Start: 1813057

End: 1814313

Strand: Direct

Name: proP1 [H]

Synonym: OTT_1789

Alternate gene names: 189184696

Gene position: 1813057-1814313 (Clockwise)

Preceding gene: 189184695

Following gene: 189184697

Centisome position: 90.25

GC content: 30.63

Gene sequence:

>1257_bases
ATGCTAAACAAAAATTACAAAATCATTCTAAGTAGCTGTATTGCTAATATCTTCGAGTGGTATGATTATACGTTATTCAT
TCATTTCTCCATTACTATTGCAAACAACTTTTTCCCAAAAGCTAATCAATCAGCTATTTTATTAGAAGCATTCTTAGTTT
TTGCAGTAGGATATTTAGTTAGGCCAATTGGAGGCATATTTTTTGGTATTATAGGAGATAAATTCGGCAGAAAAGAAGCA
GTAGCAATGTCAGTAATATGCATTTCTTTACCAACTACAATTATCGGCATTCTACCGACTTATCAATCAATAGGAATCAG
TGCTACTATTATTATCACCATAACTAGGTTGCTTCAAGGATTATCTGTTGGAGGCAATTTAACCGGTTCAGTTTCATTCT
TAATTGAACATAGCAATAAAAAAAATCATGGGCTACTTGGTAGTTTGTTGATGTCTAGCTTATGTATCGGTATATTGCTA
GGATTACTAACATCATACTTAATTAAAGCTATCCTAACTCCAGAACAGTTTAATAATTGGGGCTGGCGCATTCCATTTTT
ACTCGGCTCTCCAATAATTTTACTAGGCATTTATATTAAATATGCTATTCAGGATACTCCAATATTTAAGCAGATGCAAA
CTACTAATAAAATTGAAAAGTTTCCACTCAGAAAAACCTTGAGATTACACTGGAAAGATATATTGATTTCTATTATGATT
AACTCTACTGGATCAGTTATATTTTACTTTGAAGCAGCATATTTAATAAATTACTTAGAGTTTAATAAAGGATTTGATAA
AATAGCAATTGATTTTCTAAACAGGTGTTGCTGTATAATAATGGCTATAGTTACACTGTTTAGTGGATGGTTATATGATC
GAATAGGAGCTAAAAAATTATACACAGCAATCATTACGTTAATTATATTATCTATTGGATTCATAACTCAATTACTAGAG
CAAGAAAACTTTAATCTAGTTATTTGCGCTCAAGTTGCAATAGCGATTTTAGCAGCATTATATATAGGGGCTGAACCAGC
TATGCAAAGCGCATTGTATTCTAATAACATTAGAAACACAGCATTATCTTTATCATACAATATTTCAGTAAGTATTTTTG
GTGGCACTGCTCCAATTATTTTTGAATATTTAGTACAAACTACAGGATCTCTTAATTCTGTAGCATATTATATTACAGCT
TGCTCGCTTATAAGCTTGATAGGAATATGTTTATATAAACAAAGTTCAGATATATAA

Upstream 100 bases:

>100_bases
ATATTTGGTTTGGAGGGGCAATTTTAATTTTTTCAACTACTTTCATATTTTATACTGAAAAAGCTAATATCAATAAACAT
TAAAGATTATTAATTTTTAA

Downstream 100 bases:

>100_bases
GAAATATGATAGAATATATTAAAAATTGATCTTGCTATAGTTAAACTACTATAAAATAGTCATAATAGTTTAACTATCAC
TATGACCTAGATATGCTTTT

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MLNKNYKIILSSCIANIFEWYDYTLFIHFSITIANNFFPKANQSAILLEAFLVFAVGYLVRPIGGIFFGIIGDKFGRKEA
VAMSVICISLPTTIIGILPTYQSIGISATIIITITRLLQGLSVGGNLTGSVSFLIEHSNKKNHGLLGSLLMSSLCIGILL
GLLTSYLIKAILTPEQFNNWGWRIPFLLGSPIILLGIYIKYAIQDTPIFKQMQTTNKIEKFPLRKTLRLHWKDILISIMI
NSTGSVIFYFEAAYLINYLEFNKGFDKIAIDFLNRCCCIIMAIVTLFSGWLYDRIGAKKLYTAIITLIILSIGFITQLLE
QENFNLVICAQVAIAILAALYIGAEPAMQSALYSNNIRNTALSLSYNISVSIFGGTAPIIFEYLVQTTGSLNSVAYYITA
CSLISLIGICLYKQSSDI

Sequences:

>Translated_418_residues
MLNKNYKIILSSCIANIFEWYDYTLFIHFSITIANNFFPKANQSAILLEAFLVFAVGYLVRPIGGIFFGIIGDKFGRKEA
VAMSVICISLPTTIIGILPTYQSIGISATIIITITRLLQGLSVGGNLTGSVSFLIEHSNKKNHGLLGSLLMSSLCIGILL
GLLTSYLIKAILTPEQFNNWGWRIPFLLGSPIILLGIYIKYAIQDTPIFKQMQTTNKIEKFPLRKTLRLHWKDILISIMI
NSTGSVIFYFEAAYLINYLEFNKGFDKIAIDFLNRCCCIIMAIVTLFSGWLYDRIGAKKLYTAIITLIILSIGFITQLLE
QENFNLVICAQVAIAILAALYIGAEPAMQSALYSNNIRNTALSLSYNISVSIFGGTAPIIFEYLVQTTGSLNSVAYYITA
CSLISLIGICLYKQSSDI
>Mature_418_residues
MLNKNYKIILSSCIANIFEWYDYTLFIHFSITIANNFFPKANQSAILLEAFLVFAVGYLVRPIGGIFFGIIGDKFGRKEA
VAMSVICISLPTTIIGILPTYQSIGISATIIITITRLLQGLSVGGNLTGSVSFLIEHSNKKNHGLLGSLLMSSLCIGILL
GLLTSYLIKAILTPEQFNNWGWRIPFLLGSPIILLGIYIKYAIQDTPIFKQMQTTNKIEKFPLRKTLRLHWKDILISIMI
NSTGSVIFYFEAAYLINYLEFNKGFDKIAIDFLNRCCCIIMAIVTLFSGWLYDRIGAKKLYTAIITLIILSIGFITQLLE
QENFNLVICAQVAIAILAALYIGAEPAMQSALYSNNIRNTALSLSYNISVSIFGGTAPIIFEYLVQTTGSLNSVAYYITA
CSLISLIGICLYKQSSDI

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=428, Percent_Identity=29.6728971962617, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1788292, Length=425, Percent_Identity=28, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1789941, Length=422, Percent_Identity=27.9620853080569, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1788942, Length=428, Percent_Identity=27.803738317757, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI87082404, Length=386, Percent_Identity=22.5388601036269, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87082231, Length=224, Percent_Identity=22.3214285714286, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46355; Mature: 46355

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNKNYKIILSSCIANIFEWYDYTLFIHFSITIANNFFPKANQSAILLEAFLVFAVGYLV
CCCCCHHHHHHHHHHHHHHHHCEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
RPIGGIFFGIIGDKFGRKEAVAMSVICISLPTTIIGILPTYQSIGISATIIITITRLLQG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHH
LSVGGNLTGSVSFLIEHSNKKNHGLLGSLLMSSLCIGILLGLLTSYLIKAILTPEQFNNW
CCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC
GWRIPFLLGSPIILLGIYIKYAIQDTPIFKQMQTTNKIEKFPLRKTLRLHWKDILISIMI
CCEEHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEE
NSTGSVIFYFEAAYLINYLEFNKGFDKIAIDFLNRCCCIIMAIVTLFSGWLYDRIGAKKL
CCCCCEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTAIITLIILSIGFITQLLEQENFNLVICAQVAIAILAALYIGAEPAMQSALYSNNIRNT
HHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
ALSLSYNISVSIFGGTAPIIFEYLVQTTGSLNSVAYYITACSLISLIGICLYKQSSDI
EEEEEEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLNKNYKIILSSCIANIFEWYDYTLFIHFSITIANNFFPKANQSAILLEAFLVFAVGYLV
CCCCCHHHHHHHHHHHHHHHHCEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
RPIGGIFFGIIGDKFGRKEAVAMSVICISLPTTIIGILPTYQSIGISATIIITITRLLQG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHH
LSVGGNLTGSVSFLIEHSNKKNHGLLGSLLMSSLCIGILLGLLTSYLIKAILTPEQFNNW
CCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC
GWRIPFLLGSPIILLGIYIKYAIQDTPIFKQMQTTNKIEKFPLRKTLRLHWKDILISIMI
CCEEHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEE
NSTGSVIFYFEAAYLINYLEFNKGFDKIAIDFLNRCCCIIMAIVTLFSGWLYDRIGAKKL
CCCCCEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTAIITLIILSIGFITQLLEQENFNLVICAQVAIAILAALYIGAEPAMQSALYSNNIRNT
HHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
ALSLSYNISVSIFGGTAPIIFEYLVQTTGSLNSVAYYITACSLISLIGICLYKQSSDI
EEEEEEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA