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The map label for this gene is fmt [H]

Identifier: 189184236

GI number: 189184236

Start: 1352544

End: 1353452

Strand: Reverse

Name: fmt [H]

Synonym: OTT_1329

Alternate gene names: 189184236

Gene position: 1353452-1352544 (Counterclockwise)

Preceding gene: 189184237

Following gene: 189184234

Centisome position: 67.37

GC content: 32.89

Gene sequence:

>909_bases
ATGGGAAGCCCAGAATTCGCTATACCAGCATTAAAGGAGCTTGCTTTATCGAAGCATAATGTAATAGCTGTCTTTACCAG
CAAGCCTAAAAAGCGTGATCGCTATTTAAATATTCAAAGATCTCCCATACATAAGCTAGCTAGCGCTTTATCTATTCCTG
TTTATACACCAGATAGTTTAAAAACCAATGATGTACAAAACTTGATAGCAACTTTAGATGCAGATGTAATTGTAGTTGCA
GCTTATGGATTGATTATTCCTAAAGCAATTCTAAAAATGAAAAAATATGGATGTATTAATATTCATCCGTCAATGCTGCC
AAAATATCGCGGTGCAGCACCAATACAACGCACTATTATTAATGGCGAAAAAGAAACTGCTGTTTGTATTATTCAGATGG
ATCAAGGAGTAGATACTGGAGATATAATACTGTGTCAAAAATTCCATTTAGCTAAAAATATTTGTTTCAGTGAACTGCAT
GATCAATGTGCTAAAGTTGGAGCTAAGTTGCTAGTAAAAGCAATAAACTATATTCATACATTACCACGTATTCCGCAATC
GCAAGATAGAGCTTCTTATGCACATAAACTTTCTAAATCAGAAAGCAAAATTAATTGGTATGAAAGTGCTTATACCATAG
ATTGTAAAATTAGAGGTATGAACCCATGGCCAGGTACTTTTTTTACTTATAATAATTGCAACATTAAAGTTTTAAAAGCC
AAAATTGTAAATAATAGTCATAATTTACAACCAGGAACTGTGAAAATAGTTAATAACAATAAATTATTGGTAGCATGTCA
AGAACATTTCTTAGAACTATTGTCATTGCAGCTCCCTGGAAGGAAAGAACTGAGCTCTAGTCAGTTTTTATGTGGTTACC
ATATCTTACCAGATACTGTTTTACAATAA

Upstream 100 bases:

>100_bases
TTTTATTAGTTGACTATTTATCTAATCTAAAAAAAGAGGCTGCCTTAAACACACTATCAAAAATTAAAGATGCGGCTTAT
GATAAATGAAAATAATTTTT

Downstream 100 bases:

>100_bases
ATTAACGATGGTTAGATATACATTGTTTGTTGATATAGCCAAAAAAAAACCATGCATTAATTAATAAAACTTCTTGTATC
AATAGCTATTGCTAATAAGC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MGSPEFAIPALKELALSKHNVIAVFTSKPKKRDRYLNIQRSPIHKLASALSIPVYTPDSLKTNDVQNLIATLDADVIVVA
AYGLIIPKAILKMKKYGCINIHPSMLPKYRGAAPIQRTIINGEKETAVCIIQMDQGVDTGDIILCQKFHLAKNICFSELH
DQCAKVGAKLLVKAINYIHTLPRIPQSQDRASYAHKLSKSESKINWYESAYTIDCKIRGMNPWPGTFFTYNNCNIKVLKA
KIVNNSHNLQPGTVKIVNNNKLLVACQEHFLELLSLQLPGRKELSSSQFLCGYHILPDTVLQ

Sequences:

>Translated_302_residues
MGSPEFAIPALKELALSKHNVIAVFTSKPKKRDRYLNIQRSPIHKLASALSIPVYTPDSLKTNDVQNLIATLDADVIVVA
AYGLIIPKAILKMKKYGCINIHPSMLPKYRGAAPIQRTIINGEKETAVCIIQMDQGVDTGDIILCQKFHLAKNICFSELH
DQCAKVGAKLLVKAINYIHTLPRIPQSQDRASYAHKLSKSESKINWYESAYTIDCKIRGMNPWPGTFFTYNNCNIKVLKA
KIVNNSHNLQPGTVKIVNNNKLLVACQEHFLELLSLQLPGRKELSSSQFLCGYHILPDTVLQ
>Mature_301_residues
GSPEFAIPALKELALSKHNVIAVFTSKPKKRDRYLNIQRSPIHKLASALSIPVYTPDSLKTNDVQNLIATLDADVIVVAA
YGLIIPKAILKMKKYGCINIHPSMLPKYRGAAPIQRTIINGEKETAVCIIQMDQGVDTGDIILCQKFHLAKNICFSELHD
QCAKVGAKLLVKAINYIHTLPRIPQSQDRASYAHKLSKSESKINWYESAYTIDCKIRGMNPWPGTFFTYNNCNIKVLKAK
IVNNSHNLQPGTVKIVNNNKLLVACQEHFLELLSLQLPGRKELSSSQFLCGYHILPDTVLQ

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI238814322, Length=304, Percent_Identity=25.6578947368421, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI21614513, Length=295, Percent_Identity=27.4576271186441, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI164663775, Length=324, Percent_Identity=27.1604938271605, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1789683, Length=291, Percent_Identity=40.2061855670103, Blast_Score=231, Evalue=4e-62,
Organism=Escherichia coli, GI1788589, Length=285, Percent_Identity=28.0701754385965, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI133930964, Length=259, Percent_Identity=26.6409266409266, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28571984, Length=226, Percent_Identity=31.858407079646, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI45550868, Length=226, Percent_Identity=31.858407079646, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24585660, Length=240, Percent_Identity=27.9166666666667, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 33732; Mature: 33601

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSPEFAIPALKELALSKHNVIAVFTSKPKKRDRYLNIQRSPIHKLASALSIPVYTPDSL
CCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCEECCCCCHHHHHHHHHCCCEECCCCC
KTNDVQNLIATLDADVIVVAAYGLIIPKAILKMKKYGCINIHPSMLPKYRGAAPIQRTII
CHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHCCEEEECHHHCCCCCCCCCHHHHHC
NGEKETAVCIIQMDQGVDTGDIILCQKFHLAKNICFSELHDQCAKVGAKLLVKAINYIHT
CCCCCEEEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPRIPQSQDRASYAHKLSKSESKINWYESAYTIDCKIRGMNPWPGTFFTYNNCNIKVLKA
CCCCCCCCHHHHHHHHHHCCCCCCEEECCEEEEEEEEECCCCCCCCEEEECCCEEEEEEE
KIVNNSHNLQPGTVKIVNNNKLLVACQEHFLELLSLQLPGRKELSSSQFLCGYHILPDTV
EEECCCCCCCCCEEEEEECCEEEEEEHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCHHH
LQ
CC
>Mature Secondary Structure 
GSPEFAIPALKELALSKHNVIAVFTSKPKKRDRYLNIQRSPIHKLASALSIPVYTPDSL
CCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCEECCCCCHHHHHHHHHCCCEECCCCC
KTNDVQNLIATLDADVIVVAAYGLIIPKAILKMKKYGCINIHPSMLPKYRGAAPIQRTII
CHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHCCEEEECHHHCCCCCCCCCHHHHHC
NGEKETAVCIIQMDQGVDTGDIILCQKFHLAKNICFSELHDQCAKVGAKLLVKAINYIHT
CCCCCEEEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPRIPQSQDRASYAHKLSKSESKINWYESAYTIDCKIRGMNPWPGTFFTYNNCNIKVLKA
CCCCCCCCHHHHHHHHHHCCCCCCEEECCEEEEEEEEECCCCCCCCEEEECCCEEEEEEE
KIVNNSHNLQPGTVKIVNNNKLLVACQEHFLELLSLQLPGRKELSSSQFLCGYHILPDTV
EEECCCCCCCCCEEEEEECCEEEEEEHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCHHH
LQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA