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The map label for this gene is def [H]

Identifier: 189184237

GI number: 189184237

Start: 1353464

End: 1354009

Strand: Reverse

Name: def [H]

Synonym: OTT_1330

Alternate gene names: 189184237

Gene position: 1354009-1353464 (Counterclockwise)

Preceding gene: 189184240

Following gene: 189184236

Centisome position: 67.4

GC content: 31.32

Gene sequence:

>546_bases
ATGTCAATATTATCTCTTATAACTGCTCCTGATCCTATTTTAAAGAAAGTTGCATCTCCAGTTGATACTGTTAATGACAG
TATTAGACAACTTATGGATGATATGCTAGAAACTATGTATCACAATCATGGAGTAGGATTAGCTGCACCACAAGTAGCAG
TATCAAAAAGAATTATAGTTCTTGATCTAAGTAAAGTTGATATTAAAGAAGACAATATAACCAACAGCGAATATAAGTAT
CCATTGTTTATGGTTAATCCTATTGTTAAAGCCATATCTAATCAAACAGCAACAGCAAAAGAAGGATGTTTATCATTACC
AAAACAGGCTATTGAAGTATCTCGTTATCATGAAATTCAAGTAACTTATCTTGATTATTATAACAAGTTGACAACACTTA
ATGCTGAAGGATGGTTAGCTAGGGCTATTCAGCATGAGGTAGATCATTTAGATGGTATTTTATTAGTTGACTATTTATCT
AATCTAAAAAAAGAGGCTGCCTTAAACACACTATCAAAAATTAAAGATGCGGCTTATGATAAATGA

Upstream 100 bases:

>100_bases
TGAGTTACAATTTATGTAAATTATTGATCAACCTGAAAAATATTGTTTTTATTACTTTTTATTTTATAATCAATGAAAAA
ATAGTTTTTTGGATTTAATT

Downstream 100 bases:

>100_bases
AAATAATTTTTATGGGAAGCCCAGAATTCGCTATACCAGCATTAAAGGAGCTTGCTTTATCGAAGCATAATGTAATAGCT
GTCTTTACCAGCAAGCCTAA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MSILSLITAPDPILKKVASPVDTVNDSIRQLMDDMLETMYHNHGVGLAAPQVAVSKRIIVLDLSKVDIKEDNITNSEYKY
PLFMVNPIVKAISNQTATAKEGCLSLPKQAIEVSRYHEIQVTYLDYYNKLTTLNAEGWLARAIQHEVDHLDGILLVDYLS
NLKKEAALNTLSKIKDAAYDK

Sequences:

>Translated_181_residues
MSILSLITAPDPILKKVASPVDTVNDSIRQLMDDMLETMYHNHGVGLAAPQVAVSKRIIVLDLSKVDIKEDNITNSEYKY
PLFMVNPIVKAISNQTATAKEGCLSLPKQAIEVSRYHEIQVTYLDYYNKLTTLNAEGWLARAIQHEVDHLDGILLVDYLS
NLKKEAALNTLSKIKDAAYDK
>Mature_180_residues
SILSLITAPDPILKKVASPVDTVNDSIRQLMDDMLETMYHNHGVGLAAPQVAVSKRIIVLDLSKVDIKEDNITNSEYKYP
LFMVNPIVKAISNQTATAKEGCLSLPKQAIEVSRYHEIQVTYLDYYNKLTTLNAEGWLARAIQHEVDHLDGILLVDYLSN
LKKEAALNTLSKIKDAAYDK

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=157, Percent_Identity=31.2101910828025, Blast_Score=98, Evalue=3e-21,
Organism=Escherichia coli, GI1789682, Length=174, Percent_Identity=41.9540229885057, Blast_Score=132, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24645728, Length=159, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24645726, Length=158, Percent_Identity=31.0126582278481, Blast_Score=80, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20229; Mature: 20097

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILSLITAPDPILKKVASPVDTVNDSIRQLMDDMLETMYHNHGVGLAAPQVAVSKRIIV
CCHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCEEEE
LDLSKVDIKEDNITNSEYKYPLFMVNPIVKAISNQTATAKEGCLSLPKQAIEVSRYHEIQ
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHEEE
VTYLDYYNKLTTLNAEGWLARAIQHEVDHLDGILLVDYLSNLKKEAALNTLSKIKDAAYD
EHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
K
C
>Mature Secondary Structure 
SILSLITAPDPILKKVASPVDTVNDSIRQLMDDMLETMYHNHGVGLAAPQVAVSKRIIV
CHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCEEEE
LDLSKVDIKEDNITNSEYKYPLFMVNPIVKAISNQTATAKEGCLSLPKQAIEVSRYHEIQ
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHEEE
VTYLDYYNKLTTLNAEGWLARAIQHEVDHLDGILLVDYLSNLKKEAALNTLSKIKDAAYD
EHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA