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The map label for this gene is mdh [H]

Identifier: 189184233

GI number: 189184233

Start: 1347959

End: 1348915

Strand: Reverse

Name: mdh [H]

Synonym: OTT_1326

Alternate gene names: 189184233

Gene position: 1348915-1347959 (Counterclockwise)

Preceding gene: 189184234

Following gene: 189184232

Centisome position: 67.14

GC content: 32.71

Gene sequence:

>957_bases
ATGAGCTATAAAGCAAAAATTTCCATAATAGGAGCAGGCAATGTTGGAGCAACACTAGCTCATTTAATCGCTGTTAAGGA
GTTAGGTGATGTAGTAATAGTAGATAAAACCAAAGCAGTTGCTCAAGGTAAAGCTTTAGATATATCACAAAGCATGGGTA
TTGGTAAATCTTGTATTAACATAACTGGCACTAATAATTATCAAGAAATTCAAGATTCAAATGTTGTTATAGTAACTGCT
GGTATAGCTAGAAAACCTGGAATGTCCAGAAATGATCTAGTCAATACTAATGCTCAAATAATGATAGATATAGCAACTCA
AATTAAATACTACTCTTCAAATGCTTTGGTTATTGTCGTTACCAACCCACTAGATGCTATGGTCTGGGTTATGCAGAAAA
ACTTAAGCTTTGATCATCAAAAGGTAATAGGAATGGCTGGAGTACTTGACTCAATTAGATTTAAGTACTTCTTAAGCCAA
GAGTTTGGCGTATCAATTAATAACGTTAATGCTATGGTATTAGGTGGCCATGGTGATACTATGATACCGTTAGTAAAATA
CTCAACAATAGCAGGTATACCAATCATGGAACTAGTAAAAATGGGATGGAGCACCAAAGAAAGAATTGATCAAATAGTAC
AAAGAACTAGAGATGGAGGAAAAGAAATAGTTAGTCTCCTACAAAAAAGTTCAGCATATTATGCTCCAGCAGCAGCTACA
ATGACAATGGTTGAAAGCTATTTAAAGGATCAGAAACAAATTTTAGCATGTTCAGTTTATGTTGAAAATTATTATAGTAT
AAAATCAGAAGGTTTATATATTGGCATGCCAGTAGTAATAGGGAAAAATGGAGTTGAAAAAATTATCAAACTTGAATTAA
CTGAGATAGAACAACATGCTTTTACACAATCAGTTAATGCAGTTAGAGAATTAATTACTACTGTTCAAGAATTATAA

Upstream 100 bases:

>100_bases
AAAACGCGTCTTAAGCAGTATTAACATGACTAAAATATTTTGACATCACATTAAATGCAATACTATAATATCTGCTAAAT
TAAATAACTAAGAGGTTAAT

Downstream 100 bases:

>100_bases
TCAATTGTGCTGTAGAAAAATTATTAAAATAAATATAACCTTTAAACACTTTTATACTTTAAAGTTAATAAATACAAATC
TTTGAATTAAAAATGAAATT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSYKAKISIIGAGNVGATLAHLIAVKELGDVVIVDKTKAVAQGKALDISQSMGIGKSCINITGTNNYQEIQDSNVVIVTA
GIARKPGMSRNDLVNTNAQIMIDIATQIKYYSSNALVIVVTNPLDAMVWVMQKNLSFDHQKVIGMAGVLDSIRFKYFLSQ
EFGVSINNVNAMVLGGHGDTMIPLVKYSTIAGIPIMELVKMGWSTKERIDQIVQRTRDGGKEIVSLLQKSSAYYAPAAAT
MTMVESYLKDQKQILACSVYVENYYSIKSEGLYIGMPVVIGKNGVEKIIKLELTEIEQHAFTQSVNAVRELITTVQEL

Sequences:

>Translated_318_residues
MSYKAKISIIGAGNVGATLAHLIAVKELGDVVIVDKTKAVAQGKALDISQSMGIGKSCINITGTNNYQEIQDSNVVIVTA
GIARKPGMSRNDLVNTNAQIMIDIATQIKYYSSNALVIVVTNPLDAMVWVMQKNLSFDHQKVIGMAGVLDSIRFKYFLSQ
EFGVSINNVNAMVLGGHGDTMIPLVKYSTIAGIPIMELVKMGWSTKERIDQIVQRTRDGGKEIVSLLQKSSAYYAPAAAT
MTMVESYLKDQKQILACSVYVENYYSIKSEGLYIGMPVVIGKNGVEKIIKLELTEIEQHAFTQSVNAVRELITTVQEL
>Mature_317_residues
SYKAKISIIGAGNVGATLAHLIAVKELGDVVIVDKTKAVAQGKALDISQSMGIGKSCINITGTNNYQEIQDSNVVIVTAG
IARKPGMSRNDLVNTNAQIMIDIATQIKYYSSNALVIVVTNPLDAMVWVMQKNLSFDHQKVIGMAGVLDSIRFKYFLSQE
FGVSINNVNAMVLGGHGDTMIPLVKYSTIAGIPIMELVKMGWSTKERIDQIVQRTRDGGKEIVSLLQKSSAYYAPAAATM
TMVESYLKDQKQILACSVYVENYYSIKSEGLYIGMPVVIGKNGVEKIIKLELTEIEQHAFTQSVNAVRELITTVQEL

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=312, Percent_Identity=30.7692307692308, Blast_Score=154, Evalue=8e-38,
Organism=Homo sapiens, GI221136809, Length=312, Percent_Identity=30.7692307692308, Blast_Score=154, Evalue=8e-38,
Organism=Homo sapiens, GI9257228, Length=308, Percent_Identity=32.1428571428571, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI4504973, Length=308, Percent_Identity=32.1428571428571, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI15082234, Length=315, Percent_Identity=28.2539682539683, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI260099723, Length=306, Percent_Identity=27.7777777777778, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI5031857, Length=306, Percent_Identity=27.7777777777778, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI291575128, Length=316, Percent_Identity=27.2151898734177, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI4557032, Length=316, Percent_Identity=27.2151898734177, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI260099727, Length=219, Percent_Identity=31.5068493150685, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI260099725, Length=194, Percent_Identity=31.9587628865979, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI21735621, Length=300, Percent_Identity=26.6666666666667, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI207028494, Length=192, Percent_Identity=25.5208333333333, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI103472011, Length=290, Percent_Identity=21.7241379310345, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1789632, Length=331, Percent_Identity=26.2839879154079, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17535107, Length=286, Percent_Identity=25.5244755244755, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17554310, Length=330, Percent_Identity=29.3939393939394, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6322765, Length=305, Percent_Identity=28.1967213114754, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320125, Length=312, Percent_Identity=25.9615384615385, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324446, Length=199, Percent_Identity=28.643216080402, Blast_Score=76, Evalue=7e-15,
Organism=Drosophila melanogaster, GI17136226, Length=305, Percent_Identity=28.1967213114754, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24647881, Length=324, Percent_Identity=29.320987654321, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45550422, Length=313, Percent_Identity=24.6006389776358, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24663595, Length=280, Percent_Identity=27.5, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24663599, Length=288, Percent_Identity=27.0833333333333, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 34705; Mature: 34574

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYKAKISIIGAGNVGATLAHLIAVKELGDVVIVDKTKAVAQGKALDISQSMGIGKSCIN
CCEEEEEEEEECCCHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCEECHHHCCCCCCEEE
ITGTNNYQEIQDSNVVIVTAGIARKPGMSRNDLVNTNAQIMIDIATQIKYYSSNALVIVV
ECCCCCHHHHCCCCEEEEEECCCCCCCCCCCCEECCCCEEEEEEEEEEEEECCCEEEEEE
TNPLDAMVWVMQKNLSFDHQKVIGMAGVLDSIRFKYFLSQEFGVSINNVNAMVLGGHGDT
ECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECHHHCCEEECCEEEEECCCCCC
MIPLVKYSTIAGIPIMELVKMGWSTKERIDQIVQRTRDGGKEIVSLLQKSSAYYAPAAAT
EEEEEEHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHH
MTMVESYLKDQKQILACSVYVENYYSIKSEGLYIGMPVVIGKNGVEKIIKLELTEIEQHA
HHHHHHHHHHHHHHEEEEEEEHHHEEECCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHH
FTQSVNAVRELITTVQEL
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SYKAKISIIGAGNVGATLAHLIAVKELGDVVIVDKTKAVAQGKALDISQSMGIGKSCIN
CEEEEEEEEECCCHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCEECHHHCCCCCCEEE
ITGTNNYQEIQDSNVVIVTAGIARKPGMSRNDLVNTNAQIMIDIATQIKYYSSNALVIVV
ECCCCCHHHHCCCCEEEEEECCCCCCCCCCCCEECCCCEEEEEEEEEEEEECCCEEEEEE
TNPLDAMVWVMQKNLSFDHQKVIGMAGVLDSIRFKYFLSQEFGVSINNVNAMVLGGHGDT
ECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECHHHCCEEECCEEEEECCCCCC
MIPLVKYSTIAGIPIMELVKMGWSTKERIDQIVQRTRDGGKEIVSLLQKSSAYYAPAAAT
EEEEEEHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHH
MTMVESYLKDQKQILACSVYVENYYSIKSEGLYIGMPVVIGKNGVEKIIKLELTEIEQHA
HHHHHHHHHHHHHHEEEEEEEHHHEEECCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHH
FTQSVNAVRELITTVQEL
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA