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The map label for this gene is folC [H]

Identifier: 189183870

GI number: 189183870

Start: 1009271

End: 1010611

Strand: Reverse

Name: folC [H]

Synonym: OTT_0963

Alternate gene names: 189183870

Gene position: 1010611-1009271 (Counterclockwise)

Preceding gene: 189183877

Following gene: 189183868

Centisome position: 50.3

GC content: 31.84

Gene sequence:

>1341_bases
ATGGTAAAAATGCCGCATTGGCCAATTCCTTGGCGCAATCATATAAAATATGACCTCACTAGAATTAAATTATTACTATC
AGCTTTACAAAATCCACATCTAAAAGTACCGCCAGTCATTCATGTTGCTGGAACTAATGGAAAAGGATCTACTATAGCTT
ATTTAAGAGCAATATTTGAGACTACTGGTTATAAAGTACATAGTTATACTTCTCCACATTTACTGAATTTTAATGAAAGG
ATTACAATTGCATCTGAGTATATTGATGATAGATATCTATTTAACATTATTGAAAAAACTAGAACTACAGCAGCTAAAGT
AAATATAACACCCACTTTTTTTGAAGGCACTACTGCTGCAGCATTTCTAGCATTTGCAGAAAATAAAGCTGATATTTTAC
TATTGGAAACTGGAATGGGTGGAAGACTAGATGCAACTAACGTAATAGATAATCCTATTGCAACAATAATTACCCCAATT
TCATATGACCACATGGAATACTTGGGCCCTACCCTAACTACTATTGCTGCTGAGAAGGCAGGCATTATTAAGGCTAATGC
TCCATGCATTATTAGTTGCCAAGCGCAGCAAGTATATGATCTTCTAATAGACTATGCTATACGAGTAAAAGCTATACCTA
TAGTATATGAATATGACTTCGGTATTCAAAAACAAAATGATAGTTTCAAATTTTTATCTCATAATTTTACTTATCAATTT
TCTCTACCATCTTTACCAGGTAACCACCAAGTTATTAATGCTGCTACTGCTATCGCAACATGCAAAGCTATAACTAAGCA
TTTTAATTTTAGTGCTAATGATATTCAAAAAGGTATAAGTAATACTGTTTGGCCAGCAAGGTTAGAAAAAATTGATCTAT
TACAATATGATAAAATTTTTAACATAATTATGAAGCATTTATACAAAAAAAACATTGATGTACCACATAATATACCACAA
ATTTGGCTCGACGGTGCTCATAATACTGGCGGAGCTCAGTCATTATCTAATTTTATTAGAGAAAATCTAACACCGCCAAT
ATATTTAATTCTAGGCATGACAAGCAACAGAGAAGTTAAACCTTTTTTATCATATTTTACAGATATTATTACTCAAATTT
TTACAGTGAGTGTAATATCTGAACCTGCTAGCTATGATGCTGAAAAGCTAGCAGCATATGCTAACAGTTCTGGTATCATA
TCGATTCCTACTGAATCATTAACAGATGCTATAATTGCAGCTATTAAATCAAATCAAGCTTTTGTACAACAAAATGGTAA
TATAATCATTACTGGCTCACTATTTTTAATGGCTGATTTTTATAATCTAATTAGAGGCTAA

Upstream 100 bases:

>100_bases
GAAACTATTTACTAACAATCAAAATTGATAAGATTTGTATAATACAATCTACAATACTAAAATTCTTCAAAATTACTTTT
ATTAAGAATATAATATTAAC

Downstream 100 bases:

>100_bases
TATAGATTATTTAGTATAAAATAAATACTCTATATAAAATAACCTCTATTAAAAAAAGAAAAACTAAAAAAAGTTGCAAC
AAGTGTTTATAAAGCTCTAT

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MVKMPHWPIPWRNHIKYDLTRIKLLLSALQNPHLKVPPVIHVAGTNGKGSTIAYLRAIFETTGYKVHSYTSPHLLNFNER
ITIASEYIDDRYLFNIIEKTRTTAAKVNITPTFFEGTTAAAFLAFAENKADILLLETGMGGRLDATNVIDNPIATIITPI
SYDHMEYLGPTLTTIAAEKAGIIKANAPCIISCQAQQVYDLLIDYAIRVKAIPIVYEYDFGIQKQNDSFKFLSHNFTYQF
SLPSLPGNHQVINAATAIATCKAITKHFNFSANDIQKGISNTVWPARLEKIDLLQYDKIFNIIMKHLYKKNIDVPHNIPQ
IWLDGAHNTGGAQSLSNFIRENLTPPIYLILGMTSNREVKPFLSYFTDIITQIFTVSVISEPASYDAEKLAAYANSSGII
SIPTESLTDAIIAAIKSNQAFVQQNGNIIITGSLFLMADFYNLIRG

Sequences:

>Translated_446_residues
MVKMPHWPIPWRNHIKYDLTRIKLLLSALQNPHLKVPPVIHVAGTNGKGSTIAYLRAIFETTGYKVHSYTSPHLLNFNER
ITIASEYIDDRYLFNIIEKTRTTAAKVNITPTFFEGTTAAAFLAFAENKADILLLETGMGGRLDATNVIDNPIATIITPI
SYDHMEYLGPTLTTIAAEKAGIIKANAPCIISCQAQQVYDLLIDYAIRVKAIPIVYEYDFGIQKQNDSFKFLSHNFTYQF
SLPSLPGNHQVINAATAIATCKAITKHFNFSANDIQKGISNTVWPARLEKIDLLQYDKIFNIIMKHLYKKNIDVPHNIPQ
IWLDGAHNTGGAQSLSNFIRENLTPPIYLILGMTSNREVKPFLSYFTDIITQIFTVSVISEPASYDAEKLAAYANSSGII
SIPTESLTDAIIAAIKSNQAFVQQNGNIIITGSLFLMADFYNLIRG
>Mature_446_residues
MVKMPHWPIPWRNHIKYDLTRIKLLLSALQNPHLKVPPVIHVAGTNGKGSTIAYLRAIFETTGYKVHSYTSPHLLNFNER
ITIASEYIDDRYLFNIIEKTRTTAAKVNITPTFFEGTTAAAFLAFAENKADILLLETGMGGRLDATNVIDNPIATIITPI
SYDHMEYLGPTLTTIAAEKAGIIKANAPCIISCQAQQVYDLLIDYAIRVKAIPIVYEYDFGIQKQNDSFKFLSHNFTYQF
SLPSLPGNHQVINAATAIATCKAITKHFNFSANDIQKGISNTVWPARLEKIDLLQYDKIFNIIMKHLYKKNIDVPHNIPQ
IWLDGAHNTGGAQSLSNFIRENLTPPIYLILGMTSNREVKPFLSYFTDIITQIFTVSVISEPASYDAEKLAAYANSSGII
SIPTESLTDAIIAAIKSNQAFVQQNGNIIITGSLFLMADFYNLIRG

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=343, Percent_Identity=26.8221574344023, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI66932984, Length=343, Percent_Identity=26.8221574344023, Blast_Score=118, Evalue=9e-27,
Organism=Escherichia coli, GI1788654, Length=439, Percent_Identity=25.9681093394077, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17553150, Length=451, Percent_Identity=23.9467849223947, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71984923, Length=451, Percent_Identity=23.9467849223947, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17553148, Length=451, Percent_Identity=23.9467849223947, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323760, Length=449, Percent_Identity=27.3942093541203, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6324815, Length=450, Percent_Identity=24.6666666666667, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24641571, Length=152, Percent_Identity=35.5263157894737, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24581568, Length=202, Percent_Identity=32.1782178217822, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 49737; Mature: 49737

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKMPHWPIPWRNHIKYDLTRIKLLLSALQNPHLKVPPVIHVAGTNGKGSTIAYLRAIFE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
TTGYKVHSYTSPHLLNFNERITIASEYIDDRYLFNIIEKTRTTAAKVNITPTFFEGTTAA
HCCEEEEECCCCCEECCCCEEEEHHHHCCHHHHHHHHHHHCCCEEEEEECEEEECCCHHH
AFLAFAENKADILLLETGMGGRLDATNVIDNPIATIITPISYDHMEYLGPTLTTIAAEKA
HHEEEECCCCCEEEEECCCCCCCCCCHHHCCCHHHHEECCCHHHHHHHCCHHHHHHHHCC
GIIKANAPCIISCQAQQVYDLLIDYAIRVKAIPIVYEYDFGIQKQNDSFKFLSHNFTYQF
CEEECCCCEEEEECHHHHHHHHHHHHHEEEEEEEEEEECCCEEECCCCEEEEECCEEEEE
SLPSLPGNHQVINAATAIATCKAITKHFNFSANDIQKGISNTVWPARLEKIDLLQYDKIF
ECCCCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
NIIMKHLYKKNIDVPHNIPQIWLDGAHNTGGAQSLSNFIRENLTPPIYLILGMTSNREVK
HHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHH
PFLSYFTDIITQIFTVSVISEPASYDAEKLAAYANSSGIISIPTESLTDAIIAAIKSNQA
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEECHHHHHHHHHHHHHCCCE
FVQQNGNIIITGSLFLMADFYNLIRG
EEECCCCEEEEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MVKMPHWPIPWRNHIKYDLTRIKLLLSALQNPHLKVPPVIHVAGTNGKGSTIAYLRAIFE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
TTGYKVHSYTSPHLLNFNERITIASEYIDDRYLFNIIEKTRTTAAKVNITPTFFEGTTAA
HCCEEEEECCCCCEECCCCEEEEHHHHCCHHHHHHHHHHHCCCEEEEEECEEEECCCHHH
AFLAFAENKADILLLETGMGGRLDATNVIDNPIATIITPISYDHMEYLGPTLTTIAAEKA
HHEEEECCCCCEEEEECCCCCCCCCCHHHCCCHHHHEECCCHHHHHHHCCHHHHHHHHCC
GIIKANAPCIISCQAQQVYDLLIDYAIRVKAIPIVYEYDFGIQKQNDSFKFLSHNFTYQF
CEEECCCCEEEEECHHHHHHHHHHHHHEEEEEEEEEEECCCEEECCCCEEEEECCEEEEE
SLPSLPGNHQVINAATAIATCKAITKHFNFSANDIQKGISNTVWPARLEKIDLLQYDKIF
ECCCCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
NIIMKHLYKKNIDVPHNIPQIWLDGAHNTGGAQSLSNFIRENLTPPIYLILGMTSNREVK
HHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHH
PFLSYFTDIITQIFTVSVISEPASYDAEKLAAYANSSGIISIPTESLTDAIIAAIKSNQA
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEECHHHHHHHHHHHHHCCCE
FVQQNGNIIITGSLFLMADFYNLIRG
EEECCCCEEEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]