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The map label for this gene is kefA [H]

Identifier: 189183672

GI number: 189183672

Start: 792964

End: 793830

Strand: Direct

Name: kefA [H]

Synonym: OTT_0765

Alternate gene names: 189183672

Gene position: 792964-793830 (Clockwise)

Preceding gene: 189183670

Following gene: 189183675

Centisome position: 39.47

GC content: 28.6

Gene sequence:

>867_bases
ATGATTCAAACATTAAAAGATGCTTTAAATATTATATTGGAAATATGGAACTTTCCATTAGTTCACATCAGCCAAGATGC
TGATATTTATGTAGGCAATATATTTTTAGCTGCAGTATTATTATTATTGGGTATAAGACACTATAAAAAGTTCAAAAAGT
TTGTTAGAGTAGCTATAGTCAATCATATTACTACTGAACTTCATACAGCAAAAGTTTTAGGGAGAATATTTGAATGGTTA
ATCTCTGCTGTTTATTTAATAACAATTTTACAAATGTCTAACATTCCATTAAGCGCTTTTGCTGTTATTAGCGGAGGAGT
AGGTATAGGTATTGGGCTAGGCGCAAAAAATTTCTTTAATAATTTTATGAGTAGTGTAATTATTATGATTGAAAGACCAT
TTAAAATTGGTGATATAATCAGTGTAGCTGGAGTAAGTGGAGTTGTTAGCGCAATTGGAGGCAGATGTATTACTATCATT
AATGAAAACAATGTAAAAGTTTTAATTCCTAATAGTAAAATTATACAGGAGAATTTAATTAACTGGACTTCTGCAACTCA
GATAATAAAATGTAAGGCTGAGCTTAGAATTACTAAGGTAGGAAATGGTAAGTATAATTCAAATTGTTGCAAAGATACAA
TTAAAACTATCGAAAAAATTATGGAAGATTCTCCAATGATAAGTAATACTCCTTTGCCAAGGTGCTACTTAACTAACATT
GATTCTAGCCACTATATTTATCAACTTGAGTTTTTTTGCTATTTAACAGATATATCATCAATCTATATGGTAGAAAATGA
TCTGAGTTGTAGTATTGATAATCAATTAAAAAGTGAATTTACTATTTCATATTATACTGTAGTGTGA

Upstream 100 bases:

>100_bases
TTAGGATTACTGCAGTTTTAATTTTGATTTCTTTGTCGCTTGAAAATTAATTATTTTGTTGTTTTGCATGATGTTTGCAT
TAATAAAATATCTATAAGTT

Downstream 100 bases:

>100_bases
AGTGTTAACAAATTTCTAACTTAAAAAAGTAATAGCAATAAAAGCTAAGAAATAATGATCAAGATTGGCTAGAATCCCTA
AGAACTCTAGTATTCATATT

Product: hypothetical protein

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MIQTLKDALNIILEIWNFPLVHISQDADIYVGNIFLAAVLLLLGIRHYKKFKKFVRVAIVNHITTELHTAKVLGRIFEWL
ISAVYLITILQMSNIPLSAFAVISGGVGIGIGLGAKNFFNNFMSSVIIMIERPFKIGDIISVAGVSGVVSAIGGRCITII
NENNVKVLIPNSKIIQENLINWTSATQIIKCKAELRITKVGNGKYNSNCCKDTIKTIEKIMEDSPMISNTPLPRCYLTNI
DSSHYIYQLEFFCYLTDISSIYMVENDLSCSIDNQLKSEFTISYYTVV

Sequences:

>Translated_288_residues
MIQTLKDALNIILEIWNFPLVHISQDADIYVGNIFLAAVLLLLGIRHYKKFKKFVRVAIVNHITTELHTAKVLGRIFEWL
ISAVYLITILQMSNIPLSAFAVISGGVGIGIGLGAKNFFNNFMSSVIIMIERPFKIGDIISVAGVSGVVSAIGGRCITII
NENNVKVLIPNSKIIQENLINWTSATQIIKCKAELRITKVGNGKYNSNCCKDTIKTIEKIMEDSPMISNTPLPRCYLTNI
DSSHYIYQLEFFCYLTDISSIYMVENDLSCSIDNQLKSEFTISYYTVV
>Mature_288_residues
MIQTLKDALNIILEIWNFPLVHISQDADIYVGNIFLAAVLLLLGIRHYKKFKKFVRVAIVNHITTELHTAKVLGRIFEWL
ISAVYLITILQMSNIPLSAFAVISGGVGIGIGLGAKNFFNNFMSSVIIMIERPFKIGDIISVAGVSGVVSAIGGRCITII
NENNVKVLIPNSKIIQENLINWTSATQIIKCKAELRITKVGNGKYNSNCCKDTIKTIEKIMEDSPMISNTPLPRCYLTNI
DSSHYIYQLEFFCYLTDISSIYMVENDLSCSIDNQLKSEFTISYYTVV

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=225, Percent_Identity=23.1111111111111, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI2367355, Length=224, Percent_Identity=21.875, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 32216; Mature: 32216

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQTLKDALNIILEIWNFPLVHISQDADIYVGNIFLAAVLLLLGIRHYKKFKKFVRVAIV
CCHHHHHHHHHHHHHHCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NHITTELHTAKVLGRIFEWLISAVYLITILQMSNIPLSAFAVISGGVGIGIGLGAKNFFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEECCCEEEECCCHHHHHH
NFMSSVIIMIERPFKIGDIISVAGVSGVVSAIGGRCITIINENNVKVLIPNSKIIQENLI
HHHHHHEEHEECCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEECCCHHHHHHHC
NWTSATQIIKCKAELRITKVGNGKYNSNCCKDTIKTIEKIMEDSPMISNTPLPRCYLTNI
CHHHHHHEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECC
DSSHYIYQLEFFCYLTDISSIYMVENDLSCSIDNQLKSEFTISYYTVV
CCCCEEEEEEEEEEEECCCEEEEEECCCCCCCCHHHCCCEEEEEEEEC
>Mature Secondary Structure
MIQTLKDALNIILEIWNFPLVHISQDADIYVGNIFLAAVLLLLGIRHYKKFKKFVRVAIV
CCHHHHHHHHHHHHHHCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NHITTELHTAKVLGRIFEWLISAVYLITILQMSNIPLSAFAVISGGVGIGIGLGAKNFFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEECCCEEEECCCHHHHHH
NFMSSVIIMIERPFKIGDIISVAGVSGVVSAIGGRCITIINENNVKVLIPNSKIIQENLI
HHHHHHEEHEECCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEECCCHHHHHHHC
NWTSATQIIKCKAELRITKVGNGKYNSNCCKDTIKTIEKIMEDSPMISNTPLPRCYLTNI
CHHHHHHEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECC
DSSHYIYQLEFFCYLTDISSIYMVENDLSCSIDNQLKSEFTISYYTVV
CCCCEEEEEEEEEEEECCCEEEEEECCCCCCCCHHHCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]