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The map label for this gene is priA [H]

Identifier: 189183675

GI number: 189183675

Start: 797016

End: 798956

Strand: Direct

Name: priA [H]

Synonym: OTT_0768

Alternate gene names: 189183675

Gene position: 797016-798956 (Clockwise)

Preceding gene: 189183672

Following gene: 189183676

Centisome position: 39.67

GC content: 31.32

Gene sequence:

>1941_bases
ATGTTTATTAGAGTATTATTACCAAAGCTAAGGTTATTTCCGCTGGAGTATAAATCAACTACACATTTGTTATCCATTGG
AGATTTAGTAGTTGTTCCTTTTAGAAACAAGCAAATTACTGGTATTGTATGGGAAGTTAATATTATTCCACAACACAAAA
ACATTAAGGAAATTATAGCAAAGCAATTTCAAGGATATTCTATTAAGCCAATAAACTTAAAGTTTATTGATATTGTAGCA
AGGTACTATATAGCAGATTTAGGAACTGTAGCAAAAATGACTTTACCTATTGAATTAAATGAGAAACCATATGTTGCCGA
GTCGCAAGAGGTTAATCATAATTTATTTCAGTTGCTAGATCTGTCGCCAGAACAGCAGTTAGCATTTGATGCTATTAGAA
AAACTACAGGCGCAACCTTACTGCAAGGTATTACAGGATCAGGTAAAACAGAGGTATATTTTCATTTGATTGTTGAGGCA
TTGAGACAGGGATTACAGGTACTTCTACTAATGCCGGAAATTAATCTTGCTAGTCATATAGCATTCAGATTTGCTAGTAG
ATTTAAATTTAATGCAGTTATATGGAATTCATCTGTTAGTAAAGCCTTAAAGAAAAAAGCGCTCCGAGGAATATTAACTA
ATGAAATAAAAGTAGTTATTGGTACACGTAGTAGCATATTTTTACCATACTCATCGCTTGGATTAATTATAGTAGATGAA
GAACATGATCAATCATACAAACAAGAAAATAATATACTGTATAATGCTAGAGATATGGCGGTGATGAGAGGGCATATTGC
TAATATACCAGTAATTTTAGCTTCAGCAACTCCATCTCTTGAGAGTATATATAATACGTATAATAAGAAATACAGTTTTG
TTAAGCTTAAAAGTAGATTTGGCAGTTCTACTCTGCCTAATATCAATATTATTGATATGAAGAAGGAACATTTACCAAAT
GGAATGTGGTTATCTAGTAGCTTGAAAAATGCTATGGAGCAAACTTTAAAACTGAATAAACAAATATTGTTATTTTTAAA
CCGTAAAGGGTATGCTCCCATAATACTATGTCGTAATTGTGGTTTTCGCTTTACGTGTTCATCATGTTCTTCATGCTTGG
TTTTTCATAAGTCAAAAAATAAATTGCAATGTCATCATTGTGGTGGTTTTAAAACATTTGCTCAATTTTGTCCTGAATGT
AACGAAGAAACATTATCAGCTTATGGACCTGGAATTGAAAGAATTGCTGAGGAAGTTAAAGTATATTTTCCTAAATATAA
AACAGCAATTGTTAGCAGAGATACTACTAGTACTCTTGATGATAGTACTGAAATTTTACAGCAAATTATTAATAATGAAG
TAGATATTATAATTGGCACGCAAATGCTTACAAAAGGATACCATTTTCCAAATCTAAAACTAGTTGGAATAATTGATGCT
GACCTAGGATATGGTGGTGATTTAAGAGCTAATGAAAAGACATACCAGCTCCTTAATCAAGTAGCAGGGAGAGCTGGCAG
AGAAGAAGATAAAGGACATGTTTATATACAAACTTATTATCCTGATAGTTCACTATTAAAATATCTTATTAATTACAAAG
ATGATGATTTTATTAACTATGAAATGAATTTACGAATTAGTAATAATATGCCTCCAGCAACTAGATTTGTTGCTATTAGT
GTTATATCTACTTCTGAACAGAATGCTCAAGAGGTAGCAAAAAACATTGTTAGAACTGCTAAGCCATGTTCCAGTTTGAG
AATATTAGGCCCAGCTCCTGCGTTATTATACAAATTGAAAAATAAATATCGTTTTAGAATACTACTGATTTTTGAGCGAA
AACTAGATATTCGCAGCTACATCAAAAACTGGAATATTTTGTCATTTCACTCTAAAAGAGTTAGCTTGAAAATAGATATT
GATCCATACAATTTCTATTAA

Upstream 100 bases:

>100_bases
TGATAATTTTTAATATGTCTGCTTATTGTACAGAATTTAATATTTGAGAATAAAGTATATTAATCTATAAATATACTTTA
AGCAAGTTTATTAATAACTC

Downstream 100 bases:

>100_bases
TAACTTTAGACTTTCTGTAAAATGATAGATAGTAATATAAACATGAGTTTGATATAAGACACAGATTAGATACTGGTGCT
ATATATTGATGATGAGATAA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 646; Mature: 646

Protein sequence:

>646_residues
MFIRVLLPKLRLFPLEYKSTTHLLSIGDLVVVPFRNKQITGIVWEVNIIPQHKNIKEIIAKQFQGYSIKPINLKFIDIVA
RYYIADLGTVAKMTLPIELNEKPYVAESQEVNHNLFQLLDLSPEQQLAFDAIRKTTGATLLQGITGSGKTEVYFHLIVEA
LRQGLQVLLLMPEINLASHIAFRFASRFKFNAVIWNSSVSKALKKKALRGILTNEIKVVIGTRSSIFLPYSSLGLIIVDE
EHDQSYKQENNILYNARDMAVMRGHIANIPVILASATPSLESIYNTYNKKYSFVKLKSRFGSSTLPNINIIDMKKEHLPN
GMWLSSSLKNAMEQTLKLNKQILLFLNRKGYAPIILCRNCGFRFTCSSCSSCLVFHKSKNKLQCHHCGGFKTFAQFCPEC
NEETLSAYGPGIERIAEEVKVYFPKYKTAIVSRDTTSTLDDSTEILQQIINNEVDIIIGTQMLTKGYHFPNLKLVGIIDA
DLGYGGDLRANEKTYQLLNQVAGRAGREEDKGHVYIQTYYPDSSLLKYLINYKDDDFINYEMNLRISNNMPPATRFVAIS
VISTSEQNAQEVAKNIVRTAKPCSSLRILGPAPALLYKLKNKYRFRILLIFERKLDIRSYIKNWNILSFHSKRVSLKIDI
DPYNFY

Sequences:

>Translated_646_residues
MFIRVLLPKLRLFPLEYKSTTHLLSIGDLVVVPFRNKQITGIVWEVNIIPQHKNIKEIIAKQFQGYSIKPINLKFIDIVA
RYYIADLGTVAKMTLPIELNEKPYVAESQEVNHNLFQLLDLSPEQQLAFDAIRKTTGATLLQGITGSGKTEVYFHLIVEA
LRQGLQVLLLMPEINLASHIAFRFASRFKFNAVIWNSSVSKALKKKALRGILTNEIKVVIGTRSSIFLPYSSLGLIIVDE
EHDQSYKQENNILYNARDMAVMRGHIANIPVILASATPSLESIYNTYNKKYSFVKLKSRFGSSTLPNINIIDMKKEHLPN
GMWLSSSLKNAMEQTLKLNKQILLFLNRKGYAPIILCRNCGFRFTCSSCSSCLVFHKSKNKLQCHHCGGFKTFAQFCPEC
NEETLSAYGPGIERIAEEVKVYFPKYKTAIVSRDTTSTLDDSTEILQQIINNEVDIIIGTQMLTKGYHFPNLKLVGIIDA
DLGYGGDLRANEKTYQLLNQVAGRAGREEDKGHVYIQTYYPDSSLLKYLINYKDDDFINYEMNLRISNNMPPATRFVAIS
VISTSEQNAQEVAKNIVRTAKPCSSLRILGPAPALLYKLKNKYRFRILLIFERKLDIRSYIKNWNILSFHSKRVSLKIDI
DPYNFY
>Mature_646_residues
MFIRVLLPKLRLFPLEYKSTTHLLSIGDLVVVPFRNKQITGIVWEVNIIPQHKNIKEIIAKQFQGYSIKPINLKFIDIVA
RYYIADLGTVAKMTLPIELNEKPYVAESQEVNHNLFQLLDLSPEQQLAFDAIRKTTGATLLQGITGSGKTEVYFHLIVEA
LRQGLQVLLLMPEINLASHIAFRFASRFKFNAVIWNSSVSKALKKKALRGILTNEIKVVIGTRSSIFLPYSSLGLIIVDE
EHDQSYKQENNILYNARDMAVMRGHIANIPVILASATPSLESIYNTYNKKYSFVKLKSRFGSSTLPNINIIDMKKEHLPN
GMWLSSSLKNAMEQTLKLNKQILLFLNRKGYAPIILCRNCGFRFTCSSCSSCLVFHKSKNKLQCHHCGGFKTFAQFCPEC
NEETLSAYGPGIERIAEEVKVYFPKYKTAIVSRDTTSTLDDSTEILQQIINNEVDIIIGTQMLTKGYHFPNLKLVGIIDA
DLGYGGDLRANEKTYQLLNQVAGRAGREEDKGHVYIQTYYPDSSLLKYLINYKDDDFINYEMNLRISNNMPPATRFVAIS
VISTSEQNAQEVAKNIVRTAKPCSSLRILGPAPALLYKLKNKYRFRILLIFERKLDIRSYIKNWNILSFHSKRVSLKIDI
DPYNFY

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=537, Percent_Identity=35.9404096834264, Blast_Score=358, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 73621; Mature: 73621

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIRVLLPKLRLFPLEYKSTTHLLSIGDLVVVPFRNKQITGIVWEVNIIPQHKNIKEIIA
CEEEEEECHHHEEEECCCCCEEEEEECCEEEEEECCCEEEEEEEEEEEECCCCCHHHHHH
KQFQGYSIKPINLKFIDIVARYYIADLGTVAKMTLPIELNEKPYVAESQEVNHNLFQLLD
HHHCCCCEEECCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCHHCCCHHHHHHC
LSPEQQLAFDAIRKTTGATLLQGITGSGKTEVYFHLIVEALRQGLQVLLLMPEINLASHI
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHH
AFRFASRFKFNAVIWNSSVSKALKKKALRGILTNEIKVVIGTRSSIFLPYSSLGLIIVDE
HHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCEEEEEC
EHDQSYKQENNILYNARDMAVMRGHIANIPVILASATPSLESIYNTYNKKYSFVKLKSRF
CCCHHHHHCCCEEEECHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCEEEEEEHHHC
GSSTLPNINIIDMKKEHLPNGMWLSSSLKNAMEQTLKLNKQILLFLNRKGYAPIILCRNC
CCCCCCCCEEEECHHHHCCCCCEEHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEECC
GFRFTCSSCSSCLVFHKSKNKLQCHHCGGFKTFAQFCPECNEETLSAYGPGIERIAEEVK
CCEEECCCCCCEEEEECCCCCEEEEECCCHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHH
VYFPKYKTAIVSRDTTSTLDDSTEILQQIINNEVDIIIGTQMLTKGYHFPNLKLVGIIDA
HHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECHHHHCCCCCCCEEEEEEEEC
DLGYGGDLRANEKTYQLLNQVAGRAGREEDKGHVYIQTYYPDSSLLKYLINYKDDDFINY
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCEEEE
EMNLRISNNMPPATRFVAISVISTSEQNAQEVAKNIVRTAKPCSSLRILGPAPALLYKLK
EEEEEECCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHC
NKYRFRILLIFERKLDIRSYIKNWNILSFHSKRVSLKIDIDPYNFY
CCCEEEEEEEEECCCCHHHHHCCCCEEEEECCEEEEEEEECCCCCC
>Mature Secondary Structure
MFIRVLLPKLRLFPLEYKSTTHLLSIGDLVVVPFRNKQITGIVWEVNIIPQHKNIKEIIA
CEEEEEECHHHEEEECCCCCEEEEEECCEEEEEECCCEEEEEEEEEEEECCCCCHHHHHH
KQFQGYSIKPINLKFIDIVARYYIADLGTVAKMTLPIELNEKPYVAESQEVNHNLFQLLD
HHHCCCCEEECCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCHHCCCHHHHHHC
LSPEQQLAFDAIRKTTGATLLQGITGSGKTEVYFHLIVEALRQGLQVLLLMPEINLASHI
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHH
AFRFASRFKFNAVIWNSSVSKALKKKALRGILTNEIKVVIGTRSSIFLPYSSLGLIIVDE
HHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCEEEEEC
EHDQSYKQENNILYNARDMAVMRGHIANIPVILASATPSLESIYNTYNKKYSFVKLKSRF
CCCHHHHHCCCEEEECHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCEEEEEEHHHC
GSSTLPNINIIDMKKEHLPNGMWLSSSLKNAMEQTLKLNKQILLFLNRKGYAPIILCRNC
CCCCCCCCEEEECHHHHCCCCCEEHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEECC
GFRFTCSSCSSCLVFHKSKNKLQCHHCGGFKTFAQFCPECNEETLSAYGPGIERIAEEVK
CCEEECCCCCCEEEEECCCCCEEEEECCCHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHH
VYFPKYKTAIVSRDTTSTLDDSTEILQQIINNEVDIIIGTQMLTKGYHFPNLKLVGIIDA
HHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECHHHHCCCCCCCEEEEEEEEC
DLGYGGDLRANEKTYQLLNQVAGRAGREEDKGHVYIQTYYPDSSLLKYLINYKDDDFINY
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCEEEE
EMNLRISNNMPPATRFVAISVISTSEQNAQEVAKNIVRTAKPCSSLRILGPAPALLYKLK
EEEEEECCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHC
NKYRFRILLIFERKLDIRSYIKNWNILSFHSKRVSLKIDIDPYNFY
CCCEEEEEEEEECCCCHHHHHCCCCEEEEECCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA