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The map label for this gene is ntrB

Identifier: 189026203

GI number: 189026203

Start: 1064467

End: 1065489

Strand: Direct

Name: ntrB

Synonym: TPASS_0980

Alternate gene names: 189026203

Gene position: 1064467-1065489 (Clockwise)

Preceding gene: 189026202

Following gene: 189026204

Centisome position: 93.42

GC content: 54.55

Gene sequence:

>1023_bases
ATGCAACAACACGCCTTATATCATCCGGTTTCTATTGGCCCGTTGTCTCTCAAGGGGAATGTGTTTTTTGCTCCCGTTGC
AGGCTATTCTGACAGTGCGTTTCGTTCAATTGCCATTGAATGGGAAGCAAGCTTCACCTACACCGAAATGGTTTCGTCTG
AGGCGATGGTGCGCGATTCACTCAATACCAAACGTTTGATTCGGCGCGCGTCAAATGAGACGCATTACGCTATCCAGATT
TTTGGTTCTAATCCTGCAGTAATGGCAGAGACGGCAAAACTAATCGTCGATAGCGCGCAGCCGTCCTGTATCGACATCAA
CGCGGGATGTCCTATGCCTAAAATCACTAAAACAGGAGCCGGAGCCGCACTCACCCGAGAACCGACGCGCCTCTATGAAG
TGGTAAAGGCGGTCGCCGATGCTGTGTACGCGCAAGACGCGCGTATCCCAGTGACAGTAAAAATTCGTGCTGGGTGGGAA
GAGGCACACCTGACATGGAAGGAAGCTGCGCGTGCGGCAGTAGACGCAGGAGCACAAGCGCTTGCGTTGCACCCGCGCAC
CTGCGCGCAGTGTTACGCGGGAGAGGCAAACTGGGACATAATCGCAGACCTCGTGCAGTGCGCGCGTGGGTGGGGAGAGG
TTCCCGTGTTCGGCTCAGGGGATCTGCATGCGCCTGAAGACGCACGGGCAATGTTAGAACACACCGCATGCGCGGGGGTT
ATGTTTGCCCGCGGTGCTATGGGCAACCCGTTTATTTTCAGACAAACCCGTCAGCTTTTAACTGAAGGATACTACACGCC
CGTGACGTTTGAGCAAAAGCTACGCGCAGCCTGGCGCGAGCTTCACCTTCTGGCACAAGACGTGGGAGAAAGCTCAGCCT
GCAAGCAGATGCGCAAGCGTTTTGTTTCGTATGCAAAGGGTGAGCGGGGTAAAACGCAATGGTGTCAGCGCGCGGTGCAT
GCGTCTTCCTTCGCAGACTTTGCAGCAGTCATTCGTGACGCGTGTCCATGTATTGGTTTATAA

Upstream 100 bases:

>100_bases
GAGTTCATGGCTGAGCTCCTTGATATGGACATGCTTGAGCTTGCCGACCAGCTGTGGAAAAACAGCTGTGCGTGTTTTCA
CCTTCCTGAGTGAGCAGCAG

Downstream 100 bases:

>100_bases
GTTGCACGGCTTTTCAAACCGCGTGAAAAACGTACGCTTCCGGCGTACCCCAACTTACTTTGTCCTACAGGACGCGCAGC
TCCCTCGATAGAAAGCGTGA

Product: histidine phosphokinase/phophatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MQQHALYHPVSIGPLSLKGNVFFAPVAGYSDSAFRSIAIEWEASFTYTEMVSSEAMVRDSLNTKRLIRRASNETHYAIQI
FGSNPAVMAETAKLIVDSAQPSCIDINAGCPMPKITKTGAGAALTREPTRLYEVVKAVADAVYAQDARIPVTVKIRAGWE
EAHLTWKEAARAAVDAGAQALALHPRTCAQCYAGEANWDIIADLVQCARGWGEVPVFGSGDLHAPEDARAMLEHTACAGV
MFARGAMGNPFIFRQTRQLLTEGYYTPVTFEQKLRAAWRELHLLAQDVGESSACKQMRKRFVSYAKGERGKTQWCQRAVH
ASSFADFAAVIRDACPCIGL

Sequences:

>Translated_340_residues
MQQHALYHPVSIGPLSLKGNVFFAPVAGYSDSAFRSIAIEWEASFTYTEMVSSEAMVRDSLNTKRLIRRASNETHYAIQI
FGSNPAVMAETAKLIVDSAQPSCIDINAGCPMPKITKTGAGAALTREPTRLYEVVKAVADAVYAQDARIPVTVKIRAGWE
EAHLTWKEAARAAVDAGAQALALHPRTCAQCYAGEANWDIIADLVQCARGWGEVPVFGSGDLHAPEDARAMLEHTACAGV
MFARGAMGNPFIFRQTRQLLTEGYYTPVTFEQKLRAAWRELHLLAQDVGESSACKQMRKRFVSYAKGERGKTQWCQRAVH
ASSFADFAAVIRDACPCIGL
>Mature_340_residues
MQQHALYHPVSIGPLSLKGNVFFAPVAGYSDSAFRSIAIEWEASFTYTEMVSSEAMVRDSLNTKRLIRRASNETHYAIQI
FGSNPAVMAETAKLIVDSAQPSCIDINAGCPMPKITKTGAGAALTREPTRLYEVVKAVADAVYAQDARIPVTVKIRAGWE
EAHLTWKEAARAAVDAGAQALALHPRTCAQCYAGEANWDIIADLVQCARGWGEVPVFGSGDLHAPEDARAMLEHTACAGV
MFARGAMGNPFIFRQTRQLLTEGYYTPVTFEQKLRAAWRELHLLAQDVGESSACKQMRKRFVSYAKGERGKTQWCQRAVH
ASSFADFAAVIRDACPCIGL

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family

Homologues:

Organism=Homo sapiens, GI239788483, Length=249, Percent_Identity=30.9236947791165, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI239788462, Length=256, Percent_Identity=30.46875, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI40807366, Length=234, Percent_Identity=29.0598290598291, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI31742496, Length=238, Percent_Identity=26.4705882352941, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1789660, Length=277, Percent_Identity=35.7400722021661, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1788462, Length=245, Percent_Identity=30.2040816326531, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17543114, Length=256, Percent_Identity=28.90625, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17507177, Length=233, Percent_Identity=27.4678111587983, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25144369, Length=230, Percent_Identity=26.0869565217391, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323437, Length=232, Percent_Identity=27.5862068965517, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323433, Length=271, Percent_Identity=26.5682656826568, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=288, Percent_Identity=26.0416666666667, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24585320, Length=246, Percent_Identity=30.8943089430894, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19921524, Length=259, Percent_Identity=29.3436293436293, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24580595, Length=233, Percent_Identity=28.3261802575107, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI19920448, Length=233, Percent_Identity=28.3261802575107, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUS_TREPA (O83945)

Other databases:

- EMBL:   AE000520
- PIR:   B71258
- RefSeq:   NP_219417.1
- ProteinModelPortal:   O83945
- SMR:   O83945
- GeneID:   2611332
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0980
- NMPDR:   fig|243276.1.peg.975
- TIGR:   TP_0980
- HOGENOM:   HBG557545
- OMA:   TGCDGVL
- ProtClustDB:   CLSK218956
- BioCyc:   TPAL243276:TP_0980-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11082
- PIRSF:   PIRSF006621
- TIGRFAMs:   TIGR00737

Pfam domain/function: PF01207 Dus

EC number: NA

Molecular weight: Translated: 37162; Mature: 37162

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQHALYHPVSIGPLSLKGNVFFAPVAGYSDSAFRSIAIEWEASFTYTEMVSSEAMVRDS
CCCCCEECCCCCCCEEECCCEEEEEECCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHC
LNTKRLIRRASNETHYAIQIFGSNPAVMAETAKLIVDSAQPSCIDINAGCPMPKITKTGA
CCHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCC
GAALTREPTRLYEVVKAVADAVYAQDARIPVTVKIRAGWEEAHLTWKEAARAAVDAGAQA
CCEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHCCHHHHHHHHHHCCCCE
LALHPRTCAQCYAGEANWDIIADLVQCARGWGEVPVFGSGDLHAPEDARAMLEHTACAGV
EEECCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHH
MFARGAMGNPFIFRQTRQLLTEGYYTPVTFEQKLRAAWRELHLLAQDVGESSACKQMRKR
HHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
FVSYAKGERGKTQWCQRAVHASSFADFAAVIRDACPCIGL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQQHALYHPVSIGPLSLKGNVFFAPVAGYSDSAFRSIAIEWEASFTYTEMVSSEAMVRDS
CCCCCEECCCCCCCEEECCCEEEEEECCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHC
LNTKRLIRRASNETHYAIQIFGSNPAVMAETAKLIVDSAQPSCIDINAGCPMPKITKTGA
CCHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCC
GAALTREPTRLYEVVKAVADAVYAQDARIPVTVKIRAGWEEAHLTWKEAARAAVDAGAQA
CCEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHCCHHHHHHHHHHCCCCE
LALHPRTCAQCYAGEANWDIIADLVQCARGWGEVPVFGSGDLHAPEDARAMLEHTACAGV
EEECCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHH
MFARGAMGNPFIFRQTRQLLTEGYYTPVTFEQKLRAAWRELHLLAQDVGESSACKQMRKR
HHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
FVSYAKGERGKTQWCQRAVHASSFADFAAVIRDACPCIGL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876