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The map label for this gene is pleD [H]

Identifier: 189026204

GI number: 189026204

Start: 1065549

End: 1066712

Strand: Direct

Name: pleD [H]

Synonym: TPASS_0981

Alternate gene names: 189026204

Gene position: 1065549-1066712 (Clockwise)

Preceding gene: 189026203

Following gene: 189026205

Centisome position: 93.51

GC content: 48.97

Gene sequence:

>1164_bases
TTGTCCTACAGGACGCGCAGCTCCCTCGATAGAAAGCGTGACTATATCTGTCCTGCGTGCAACTTGTACAAAGCCGGTTC
CTGTGCCAGAATCGTGCATCCGCGGTGGCAGGGAATCCTGGTGAAAGAATGTGTTTCTAAAGAAAAGCGAATCTATGACC
TCAAGCAGCTCCTAGAGATTTCTAAGAGTTTGAATTCTCTCCTTGAGTTTACTCACCTGGTAGAAGCCATCCTCTACGTC
GCGATGGCCCAGACCAAGACGCTGGGGGCAGCGCTTTTCACCAAGAAAAACGCCGGTATGAAAAAATTGTCTTTGAGCCG
CAATGTGTGCGGCTTTGACGTTTCCCACCATGCACAGCTGATAATCTCGGAAGAGGACCCTATTCTCAGACTTCTGGACG
AAAAGGCCTGTTGTCTTTCTCCCGAAGAGGTACAGAGCGCGCTCGCCCCCTCAAAGAGCGTACGTTCGCTCCTTGACTTG
CAACCTTCGCTCTTTGTTCCACTAAGAGCAAAGGACCACCTTGTTGGTCTTATCCTTTTAGGCAAGAAAATCAACGTACA
CGAAGCCTACACTCCCTACGATCAGAGCATCATCATGGATATTGCACAGCTTGCTGCTATTGCCATCAACAATGCGTTAC
TGCTTGAGCAAGCTACCACTGACATGATGACCCAGATGAAGCTCAAACACTACTTCTTTGCCATGCTCACCGCGAAGCTC
GATACACTCAGTACACAAGAGACCGTATCTGTTCTCATGCTTGATATCGACTTTTTCAAACAGATCAACGACACGCACGG
TCATCTGTGTGGCGATCTAGTTCTCCAACATGTGGCAGAAATTATTCGATCCTGCACCCGTCCATGCGACATCGCCTCTC
GCTATGGGGGAGAAGAATTTATGCTCATGCTATCCAACAACTCGTCTCGGGAAGCTGCGCACGTTGCAGAACGCATTCGC
GTGGCAACCGAGCAATTGACCATCCCCTACCATGAGGTATCAATTCGAGTCACTGTTTCTGCAGGCGTCGCAGAATACCT
TCCTAACCAAGAATCCGCCGAAACACTGATAAAGCGTGCAGACAGTGCGCTGTATCAAGCCAAACAAAATGGCAGAAACA
AAGTCGTCATCTCAGAGAAAAACATGTGCTCATCTCAGGAATAA

Upstream 100 bases:

>100_bases
CAGCAGTCATTCGTGACGCGTGTCCATGTATTGGTTTATAAGTTGCACGGCTTTTCAAACCGCGTGAAAAACGTACGCTT
CCGGCGTACCCCAACTTACT

Downstream 100 bases:

>100_bases
ACCGATACTGGCGGCATGAGTGTGATCAGGAAGCCCTTCAGGTACTCGTACACCAATGTGACCCTTTCCCTTGTGCTCGC
GAATGGGGCGGTGTTTGTGA

Product: sensory transduction histidine kinase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MSYRTRSSLDRKRDYICPACNLYKAGSCARIVHPRWQGILVKECVSKEKRIYDLKQLLEISKSLNSLLEFTHLVEAILYV
AMAQTKTLGAALFTKKNAGMKKLSLSRNVCGFDVSHHAQLIISEEDPILRLLDEKACCLSPEEVQSALAPSKSVRSLLDL
QPSLFVPLRAKDHLVGLILLGKKINVHEAYTPYDQSIIMDIAQLAAIAINNALLLEQATTDMMTQMKLKHYFFAMLTAKL
DTLSTQETVSVLMLDIDFFKQINDTHGHLCGDLVLQHVAEIIRSCTRPCDIASRYGGEEFMLMLSNNSSREAAHVAERIR
VATEQLTIPYHEVSIRVTVSAGVAEYLPNQESAETLIKRADSALYQAKQNGRNKVVISEKNMCSSQE

Sequences:

>Translated_387_residues
MSYRTRSSLDRKRDYICPACNLYKAGSCARIVHPRWQGILVKECVSKEKRIYDLKQLLEISKSLNSLLEFTHLVEAILYV
AMAQTKTLGAALFTKKNAGMKKLSLSRNVCGFDVSHHAQLIISEEDPILRLLDEKACCLSPEEVQSALAPSKSVRSLLDL
QPSLFVPLRAKDHLVGLILLGKKINVHEAYTPYDQSIIMDIAQLAAIAINNALLLEQATTDMMTQMKLKHYFFAMLTAKL
DTLSTQETVSVLMLDIDFFKQINDTHGHLCGDLVLQHVAEIIRSCTRPCDIASRYGGEEFMLMLSNNSSREAAHVAERIR
VATEQLTIPYHEVSIRVTVSAGVAEYLPNQESAETLIKRADSALYQAKQNGRNKVVISEKNMCSSQE
>Mature_386_residues
SYRTRSSLDRKRDYICPACNLYKAGSCARIVHPRWQGILVKECVSKEKRIYDLKQLLEISKSLNSLLEFTHLVEAILYVA
MAQTKTLGAALFTKKNAGMKKLSLSRNVCGFDVSHHAQLIISEEDPILRLLDEKACCLSPEEVQSALAPSKSVRSLLDLQ
PSLFVPLRAKDHLVGLILLGKKINVHEAYTPYDQSIIMDIAQLAAIAINNALLLEQATTDMMTQMKLKHYFFAMLTAKLD
TLSTQETVSVLMLDIDFFKQINDTHGHLCGDLVLQHVAEIIRSCTRPCDIASRYGGEEFMLMLSNNSSREAAHVAERIRV
ATEQLTIPYHEVSIRVTVSAGVAEYLPNQESAETLIKRADSALYQAKQNGRNKVVISEKNMCSSQE

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=172, Percent_Identity=40.1162790697674, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787816, Length=135, Percent_Identity=40.7407407407407, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87081881, Length=160, Percent_Identity=40, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87082007, Length=171, Percent_Identity=40.9356725146199, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI145693134, Length=134, Percent_Identity=38.0597014925373, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787802, Length=176, Percent_Identity=32.3863636363636, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788381, Length=213, Percent_Identity=28.6384976525822, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786584, Length=163, Percent_Identity=31.2883435582822, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788085, Length=162, Percent_Identity=33.9506172839506, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787541, Length=136, Percent_Identity=33.0882352941176, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI87081977, Length=165, Percent_Identity=30.9090909090909, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787056, Length=126, Percent_Identity=34.9206349206349, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081974, Length=156, Percent_Identity=30.7692307692308, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788956, Length=171, Percent_Identity=31.5789473684211, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 43332; Mature: 43201

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYRTRSSLDRKRDYICPACNLYKAGSCARIVHPRWQGILVKECVSKEKRIYDLKQLLEI
CCCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SKSLNSLLEFTHLVEAILYVAMAQTKTLGAALFTKKNAGMKKLSLSRNVCGFDVSHHAQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCEEE
IISEEDPILRLLDEKACCLSPEEVQSALAPSKSVRSLLDLQPSLFVPLRAKDHLVGLILL
EEECCCHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCEEEEEEE
GKKINVHEAYTPYDQSIIMDIAQLAAIAINNALLLEQATTDMMTQMKLKHYFFAMLTAKL
CCEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHH
DTLSTQETVSVLMLDIDFFKQINDTHGHLCGDLVLQHVAEIIRSCTRPCDIASRYGGEEF
HHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEE
MLMLSNNSSREAAHVAERIRVATEQLTIPYHEVSIRVTVSAGVAEYLPNQESAETLIKRA
EEEEECCCCHHHHHHHHHHHHHHHHEECCEEEEEEEEEEECCHHHHCCCCHHHHHHHHHH
DSALYQAKQNGRNKVVISEKNMCSSQE
HHHHHHHHHCCCCEEEEECCCCCCCCC
>Mature Secondary Structure 
SYRTRSSLDRKRDYICPACNLYKAGSCARIVHPRWQGILVKECVSKEKRIYDLKQLLEI
CCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SKSLNSLLEFTHLVEAILYVAMAQTKTLGAALFTKKNAGMKKLSLSRNVCGFDVSHHAQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCEEE
IISEEDPILRLLDEKACCLSPEEVQSALAPSKSVRSLLDLQPSLFVPLRAKDHLVGLILL
EEECCCHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCEEEEEEE
GKKINVHEAYTPYDQSIIMDIAQLAAIAINNALLLEQATTDMMTQMKLKHYFFAMLTAKL
CCEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHH
DTLSTQETVSVLMLDIDFFKQINDTHGHLCGDLVLQHVAEIIRSCTRPCDIASRYGGEEF
HHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEE
MLMLSNNSSREAAHVAERIRVATEQLTIPYHEVSIRVTVSAGVAEYLPNQESAETLIKRA
EEEEECCCCHHHHHHHHHHHHHHHHEECCEEEEEEEEEEECCHHHHCCCCHHHHHHHHHH
DSALYQAKQNGRNKVVISEKNMCSSQE
HHHHHHHHHCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA