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The map label for this gene is ftsJ

Identifier: 189025907

GI number: 189025907

Start: 747628

End: 748230

Strand: Reverse

Name: ftsJ

Synonym: TPASS_0682

Alternate gene names: 189025907

Gene position: 748230-747628 (Counterclockwise)

Preceding gene: 189025912

Following gene: 189025906

Centisome position: 65.67

GC content: 57.05

Gene sequence:

>603_bases
GTGAACGTCTATAAACGTGCTGATTTTTGGGCAAAAAAGGCAGCCGCCGCGGGGTACCGCGCGCGTTCGGTGTATAAATT
AGCGGCGCTTGATAAAAAATACTCCCTCCTGTCGCGCGCCTCGCGGGTGCTCGATTTGGGCGCCGCGCCAGGAAGCTGGA
CCCAGTACGTGCTGGGCACCGCTGCTGCGTGCACCGCCGTGTGTGCGGTGGATGTGCAGCCGATTGCGTCGGACATTCAG
GACGCGCGTTTGCAACGAGTGCAGGGGGATTTGTGCGCAGCAGATACACGTGCGCGTGTTGCGTGCAACGCTCCTTTCGA
TCTTATTCTTTCTGATGCCGCACCTCGTACGACCGGAAACCGCACAGTAGATACGAGCGCTTCTGCGTGTCTTGCAGCAG
GGGTGTGTGCGTACGTCAACTTCTTGTCCTCTGATGGAGGATTGGTGTTCAAGGTGTTCCAAGGGTCAGAGCACCTTGCT
ATCCTTACACACCTGCGTGCGCATTTCGGTGCGGTGTGTAGTTTTAAACCGCCTGCTTCTCGTCCCCGTAGTTGTGAGTT
ATATGTGGTGGCGCGTTTCTTTCGCGGTACGTGCGGCAAGTAA

Upstream 100 bases:

>100_bases
CGTTTCATAGTACCGTTATACGGCGTGTTTGCCTTTTGTGCGAGCACGATGTGTGGGAGCTGTCAGTGTAACACATAGCA
TGGGGGGCGTGTAGGAAGGT

Downstream 100 bases:

>100_bases
TGGGTAAGAATAGGGAGCGTAGGCGCCTGTGGCATGCAATCAGGCATTGATCCATTTAGCAAACTTGCGTCATAACCTCG
GTGAAATTATGAGCCGTACA

Product: cell division protein

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MNVYKRADFWAKKAAAAGYRARSVYKLAALDKKYSLLSRASRVLDLGAAPGSWTQYVLGTAAACTAVCAVDVQPIASDIQ
DARLQRVQGDLCAADTRARVACNAPFDLILSDAAPRTTGNRTVDTSASACLAAGVCAYVNFLSSDGGLVFKVFQGSEHLA
ILTHLRAHFGAVCSFKPPASRPRSCELYVVARFFRGTCGK

Sequences:

>Translated_200_residues
MNVYKRADFWAKKAAAAGYRARSVYKLAALDKKYSLLSRASRVLDLGAAPGSWTQYVLGTAAACTAVCAVDVQPIASDIQ
DARLQRVQGDLCAADTRARVACNAPFDLILSDAAPRTTGNRTVDTSASACLAAGVCAYVNFLSSDGGLVFKVFQGSEHLA
ILTHLRAHFGAVCSFKPPASRPRSCELYVVARFFRGTCGK
>Mature_200_residues
MNVYKRADFWAKKAAAAGYRARSVYKLAALDKKYSLLSRASRVLDLGAAPGSWTQYVLGTAAACTAVCAVDVQPIASDIQ
DARLQRVQGDLCAADTRARVACNAPFDLILSDAAPRTTGNRTVDTSASACLAAGVCAYVNFLSSDGGLVFKVFQGSEHLA
ILTHLRAHFGAVCSFKPPASRPRSCELYVVARFFRGTCGK

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI29029591, Length=199, Percent_Identity=32.1608040201005, Blast_Score=88, Evalue=5e-18,
Organism=Homo sapiens, GI29029589, Length=199, Percent_Identity=32.1608040201005, Blast_Score=88, Evalue=5e-18,
Organism=Homo sapiens, GI7110661, Length=199, Percent_Identity=32.1608040201005, Blast_Score=88, Evalue=5e-18,
Organism=Homo sapiens, GI194097365, Length=191, Percent_Identity=29.8429319371728, Blast_Score=84, Evalue=8e-17,
Organism=Homo sapiens, GI7019377, Length=210, Percent_Identity=31.9047619047619, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1789569, Length=189, Percent_Identity=31.7460317460317, Blast_Score=87, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI71987550, Length=195, Percent_Identity=30.2564102564103, Blast_Score=90, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI71987561, Length=195, Percent_Identity=30.2564102564103, Blast_Score=90, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17553860, Length=195, Percent_Identity=30.2564102564103, Blast_Score=90, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17554650, Length=194, Percent_Identity=29.3814432989691, Blast_Score=86, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17553474, Length=198, Percent_Identity=32.3232323232323, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=29.1457286432161, Blast_Score=79, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6319796, Length=201, Percent_Identity=29.8507462686567, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24647580, Length=206, Percent_Identity=29.6116504854369, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648639, Length=201, Percent_Identity=30.3482587064677, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI18859957, Length=194, Percent_Identity=31.4432989690722, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21356387, Length=206, Percent_Identity=32.0388349514563, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_TREPA (O83687)

Other databases:

- EMBL:   AE000520
- PIR:   B71295
- RefSeq:   NP_219119.1
- ProteinModelPortal:   O83687
- SMR:   O83687
- GeneID:   2611272
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0682
- NMPDR:   fig|243276.1.peg.678
- TIGR:   TP_0682
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- ProtClustDB:   CLSK2300268
- BioCyc:   TPAL243276:TP_0682-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 21318; Mature: 21318

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: ACT_SITE 151-151 BINDING 51-51 BINDING 53-53 BINDING 71-71 BINDING 90-90 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVYKRADFWAKKAAAAGYRARSVYKLAALDKKYSLLSRASRVLDLGAAPGSWTQYVLGT
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
AAACTAVCAVDVQPIASDIQDARLQRVQGDLCAADTRARVACNAPFDLILSDAAPRTTGN
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHCCCCCCEEEECCCHHEEECCCCCCCCCC
RTVDTSASACLAAGVCAYVNFLSSDGGLVFKVFQGSEHLAILTHLRAHFGAVCSFKPPAS
CEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCEECCCCCCC
RPRSCELYVVARFFRGTCGK
CCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MNVYKRADFWAKKAAAAGYRARSVYKLAALDKKYSLLSRASRVLDLGAAPGSWTQYVLGT
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
AAACTAVCAVDVQPIASDIQDARLQRVQGDLCAADTRARVACNAPFDLILSDAAPRTTGN
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHCCCCCCEEEECCCHHEEECCCCCCCCCC
RTVDTSASACLAAGVCAYVNFLSSDGGLVFKVFQGSEHLAILTHLRAHFGAVCSFKPPAS
CEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCEECCCCCCC
RPRSCELYVVARFFRGTCGK
CCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876