Click here to switch to the map view.

The map label for this gene is atpB2

Identifier: 189025757

GI number: 189025757

Start: 572354

End: 573796

Strand: Reverse

Name: atpB2

Synonym: TPASS_0528

Alternate gene names: 189025757

Gene position: 573796-572354 (Counterclockwise)

Preceding gene: 189025758

Following gene: 189025756

Centisome position: 50.36

GC content: 51.98

Gene sequence:

>1443_bases
ATGAAGGGAGTGTGGTATCGGGGTCTGTCCTCCATCGACGGTCCGATCGTGGTGGCAAAGCGCCGGGAAGGTGCATTCTA
TGGGGAGATTACGGCCATCCGTGATCGCTTCGGTGCTCTGCGTACCGGCAGGATAATTGATCTTTCTCAAGAGTGTTGTC
TGATTCAGGTGTTTGGCTCCACGCTTGGGCTCAGCCTCGACGGTGCCTGCCTTGAGTTTTTGGACGTGCCGATGCAGCTG
CGTGTCTGTGAGGGTTTGATGGGGCGGGTATTCGATGGATTAGGGAGACCAATCGATGGTTTCCCAGAGGTGCTCTCTTC
TCAATTGCGTAATGTGAACGGCTATCCTATCAATCCGTACGCGCGCGTATATCCACGTGACTTCATTCAAACCGGTATTT
CTGCTATCGATGGTATGAATACGCTCATTCGTGGGCAGAAACTGCCAATCTTCTCTGGGAACGGCCTTGCGCACAACCGT
TTAGCAGCGCAGATTATCAGACAGGCAAAAATTCTTGGCACGGATGAGGCCTTTGTGATGGTATTCGCGGGTATGGGTAT
TAAGCACGATGTGGCCCGCTTTTTTGTTTCTTCTTTTGAAGAAACAGGGGTACTGTCAAAGGTGGTGATGTTCCTGTCGC
TTGCAGATGCGCCATCTATCGAGCGTATTATCACACCACGCTGTGCATTAACCGCAGCTGAGTATCTCGCCTTTGAAAAG
AACAAGCATGTATTAGTCATTTTTACAGACATGACAAACTACTGTGAGGCGCTGCGGGAAGTTTCCACCACACGAGGGGA
GGTACCCGGGCGTAAGGGTTATCCGGGTTACCTGTATTCTGATTTGGCAGAACTGTACGAGCGCGCAGGCAGAGTGAAAG
GATCCTCCGGTTCGGTGACGCAGATTCCGATCTTAACTATGCCGAACGACGATATTAGCCATCCGATCCCTGACCTGACC
GGGTACATCACCGAAGGACAGATTGTGTTGCAACGCGACCTATCTCAGCGGGGCTTGTATCCGCCCATTGGGTGTCTACC
CAGCCTATCTCGCTTAATGAAAGATGGTATCGGGGAGGGTATGACACGCGCAGATCACCATGCGGTTTCAAGTCAGCTAT
TTGCTTCATACGCAAGAGTACAAAGCGTACGGAGCCTTGCCTCGATTGTCGGAGAAGAGGAATTACCTGCACTCGATAAG
TGTTATCTGCGCTTTGGTGACTTGTTTGAGCAGTACTTTCTCACGCAGGATGAGCATGAAGATCGGAGTATCAGTCAGAC
GCTCGATATCGGGTGGAGTTTGCTCTCACTTTTGCCGCGCACCGAGCTATATCGTATCGACCCAAAGCTTATCGATCAGT
ACCTGACCGCTTCGTGCAGCGCGGTGAGTGATCAGTTGCGAAAGGCGATAGAGGAGGCCCGCACCCCGGTTGCGGACGCG
TAA

Upstream 100 bases:

>100_bases
ATGTACACAAGATGCAGAAAGTGTACGACACGATGTGCACTGAGTTTGACCAACTGAGTGTGTGTGCTGCCGCGCGCACA
CAAGGGGGGGAGAAAGTCGA

Downstream 100 bases:

>100_bases
AGACCATGTGTCCTATAAGGCTCTTGGAGAAGGGTGATTTCTTTGCGGCGCTCCCTTGCTGTGTGTCTTGGCCACGCAGG
GAGAGGATACAGAGGTGAAA

Product: V-type ATP synthase subunit B

Products: ADP; phosphate; H+

Alternate protein names: V-ATPase subunit B 2

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MKGVWYRGLSSIDGPIVVAKRREGAFYGEITAIRDRFGALRTGRIIDLSQECCLIQVFGSTLGLSLDGACLEFLDVPMQL
RVCEGLMGRVFDGLGRPIDGFPEVLSSQLRNVNGYPINPYARVYPRDFIQTGISAIDGMNTLIRGQKLPIFSGNGLAHNR
LAAQIIRQAKILGTDEAFVMVFAGMGIKHDVARFFVSSFEETGVLSKVVMFLSLADAPSIERIITPRCALTAAEYLAFEK
NKHVLVIFTDMTNYCEALREVSTTRGEVPGRKGYPGYLYSDLAELYERAGRVKGSSGSVTQIPILTMPNDDISHPIPDLT
GYITEGQIVLQRDLSQRGLYPPIGCLPSLSRLMKDGIGEGMTRADHHAVSSQLFASYARVQSVRSLASIVGEEELPALDK
CYLRFGDLFEQYFLTQDEHEDRSISQTLDIGWSLLSLLPRTELYRIDPKLIDQYLTASCSAVSDQLRKAIEEARTPVADA

Sequences:

>Translated_480_residues
MKGVWYRGLSSIDGPIVVAKRREGAFYGEITAIRDRFGALRTGRIIDLSQECCLIQVFGSTLGLSLDGACLEFLDVPMQL
RVCEGLMGRVFDGLGRPIDGFPEVLSSQLRNVNGYPINPYARVYPRDFIQTGISAIDGMNTLIRGQKLPIFSGNGLAHNR
LAAQIIRQAKILGTDEAFVMVFAGMGIKHDVARFFVSSFEETGVLSKVVMFLSLADAPSIERIITPRCALTAAEYLAFEK
NKHVLVIFTDMTNYCEALREVSTTRGEVPGRKGYPGYLYSDLAELYERAGRVKGSSGSVTQIPILTMPNDDISHPIPDLT
GYITEGQIVLQRDLSQRGLYPPIGCLPSLSRLMKDGIGEGMTRADHHAVSSQLFASYARVQSVRSLASIVGEEELPALDK
CYLRFGDLFEQYFLTQDEHEDRSISQTLDIGWSLLSLLPRTELYRIDPKLIDQYLTASCSAVSDQLRKAIEEARTPVADA
>Mature_480_residues
MKGVWYRGLSSIDGPIVVAKRREGAFYGEITAIRDRFGALRTGRIIDLSQECCLIQVFGSTLGLSLDGACLEFLDVPMQL
RVCEGLMGRVFDGLGRPIDGFPEVLSSQLRNVNGYPINPYARVYPRDFIQTGISAIDGMNTLIRGQKLPIFSGNGLAHNR
LAAQIIRQAKILGTDEAFVMVFAGMGIKHDVARFFVSSFEETGVLSKVVMFLSLADAPSIERIITPRCALTAAEYLAFEK
NKHVLVIFTDMTNYCEALREVSTTRGEVPGRKGYPGYLYSDLAELYERAGRVKGSSGSVTQIPILTMPNDDISHPIPDLT
GYITEGQIVLQRDLSQRGLYPPIGCLPSLSRLMKDGIGEGMTRADHHAVSSQLFASYARVQSVRSLASIVGEEELPALDK
CYLRFGDLFEQYFLTQDEHEDRSISQTLDIGWSLLSLLPRTELYRIDPKLIDQYLTASCSAVSDQLRKAIEEARTPVADA

Specific function: Produces ATP from ADP in the presence of a proton gradient across the membrane. The V-type beta chain is a regulatory subunit

COG id: COG1156

COG function: function code C; Archaeal/vacuolar-type H+-ATPase subunit B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATPase alpha/beta chains family

Homologues:

Organism=Homo sapiens, GI19913426, Length=461, Percent_Identity=51.8438177874187, Blast_Score=493, Evalue=1e-139,
Organism=Homo sapiens, GI19913428, Length=457, Percent_Identity=52.0787746170678, Blast_Score=489, Evalue=1e-138,
Organism=Homo sapiens, GI32189394, Length=347, Percent_Identity=25.9365994236311, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI50345984, Length=399, Percent_Identity=25.8145363408521, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI4757810, Length=399, Percent_Identity=25.8145363408521, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI19913424, Length=224, Percent_Identity=29.4642857142857, Blast_Score=83, Evalue=6e-16,
Organism=Escherichia coli, GI1788251, Length=333, Percent_Identity=30.3303303303303, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1790170, Length=364, Percent_Identity=25.8241758241758, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790172, Length=332, Percent_Identity=27.710843373494, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17570191, Length=455, Percent_Identity=51.6483516483517, Blast_Score=492, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI17510931, Length=455, Percent_Identity=50.3296703296703, Blast_Score=474, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI71988080, Length=286, Percent_Identity=28.3216783216783, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71988063, Length=286, Percent_Identity=28.3216783216783, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI25144756, Length=347, Percent_Identity=25.0720461095101, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71988074, Length=265, Percent_Identity=26.7924528301887, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17565854, Length=223, Percent_Identity=29.1479820627803, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319603, Length=457, Percent_Identity=51.8599562363239, Blast_Score=508, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6322581, Length=345, Percent_Identity=26.6666666666667, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6319370, Length=288, Percent_Identity=29.1666666666667, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320016, Length=222, Percent_Identity=25.2252252252252, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281361666, Length=458, Percent_Identity=51.3100436681223, Blast_Score=489, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24646341, Length=458, Percent_Identity=51.3100436681223, Blast_Score=489, Evalue=1e-138,
Organism=Drosophila melanogaster, GI17136796, Length=458, Percent_Identity=51.3100436681223, Blast_Score=489, Evalue=1e-138,
Organism=Drosophila melanogaster, GI28574560, Length=347, Percent_Identity=28.2420749279539, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24658560, Length=286, Percent_Identity=29.020979020979, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24638766, Length=347, Percent_Identity=25.6484149855908, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24583992, Length=224, Percent_Identity=27.2321428571429, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI20129479, Length=322, Percent_Identity=26.0869565217391, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583988, Length=224, Percent_Identity=27.2321428571429, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24583986, Length=224, Percent_Identity=27.2321428571429, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24583984, Length=224, Percent_Identity=27.2321428571429, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): VATB2_TREPA (O83540)

Other databases:

- EMBL:   AE000520
- PIR:   B71313
- RefSeq:   NP_218968.1
- ProteinModelPortal:   O83540
- SMR:   O83540
- IntAct:   O83540
- GeneID:   2610778
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0528
- NMPDR:   fig|243276.1.peg.527
- TIGR:   TP_0528
- HOGENOM:   HBG565875
- OMA:   DGIGEGY
- ProtClustDB:   PRK04196
- BioCyc:   TPAL243276:TP_0528-MONOMER
- BRENDA:   3.6.3.14
- HAMAP:   MF_00310
- InterPro:   IPR000194
- InterPro:   IPR000793
- InterPro:   IPR004100
- InterPro:   IPR022879

Pfam domain/function: PF00006 ATP-synt_ab; PF00306 ATP-synt_ab_C; PF02874 ATP-synt_ab_N

EC number: 3.6.3.14

Molecular weight: Translated: 53058; Mature: 53058

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGVWYRGLSSIDGPIVVAKRREGAFYGEITAIRDRFGALRTGRIIDLSQECCLIQVFGS
CCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHCCCEEECCCCHHHHHHHHH
TLGLSLDGACLEFLDVPMQLRVCEGLMGRVFDGLGRPIDGFPEVLSSQLRNVNGYPINPY
HHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCH
ARVYPRDFIQTGISAIDGMNTLIRGQKLPIFSGNGLAHNRLAAQIIRQAKILGTDEAFVM
HHHCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCHHHHH
VFAGMGIKHDVARFFVSSFEETGVLSKVVMFLSLADAPSIERIITPRCALTAAEYLAFEK
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHCC
NKHVLVIFTDMTNYCEALREVSTTRGEVPGRKGYPGYLYSDLAELYERAGRVKGSSGSVT
CCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEE
QIPILTMPNDDISHPIPDLTGYITEGQIVLQRDLSQRGLYPPIGCLPSLSRLMKDGIGEG
EEEEEECCCCCCCCCCCCHHCEECCCEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
MTRADHHAVSSQLFASYARVQSVRSLASIVGEEELPALDKCYLRFGDLFEQYFLTQDEHE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
DRSISQTLDIGWSLLSLLPRTELYRIDPKLIDQYLTASCSAVSDQLRKAIEEARTPVADA
HHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKGVWYRGLSSIDGPIVVAKRREGAFYGEITAIRDRFGALRTGRIIDLSQECCLIQVFGS
CCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHCCCEEECCCCHHHHHHHHH
TLGLSLDGACLEFLDVPMQLRVCEGLMGRVFDGLGRPIDGFPEVLSSQLRNVNGYPINPY
HHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCH
ARVYPRDFIQTGISAIDGMNTLIRGQKLPIFSGNGLAHNRLAAQIIRQAKILGTDEAFVM
HHHCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCHHHHH
VFAGMGIKHDVARFFVSSFEETGVLSKVVMFLSLADAPSIERIITPRCALTAAEYLAFEK
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHCC
NKHVLVIFTDMTNYCEALREVSTTRGEVPGRKGYPGYLYSDLAELYERAGRVKGSSGSVT
CCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEE
QIPILTMPNDDISHPIPDLTGYITEGQIVLQRDLSQRGLYPPIGCLPSLSRLMKDGIGEG
EEEEEECCCCCCCCCCCCHHCEECCCEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
MTRADHHAVSSQLFASYARVQSVRSLASIVGEEELPALDKCYLRFGDLFEQYFLTQDEHE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
DRSISQTLDIGWSLLSLLPRTELYRIDPKLIDQYLTASCSAVSDQLRKAIEEARTPVADA
HHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Borate; Diphosphate; HCO3- [C]

Metal ions: Co2+; Fe2+; Mn2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O; H+

Specific reaction: ATP + H2O + H+(in) = ADP + phosphate + H+(out)

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: Ca2+; CN-; Efrapeptin; Ethidiumbromide; Guanidines various reactions catalyzed by ATPase, analogs; Oligomycin; Quercetin; Trialkyl tinderivatives; Venturicidin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876