Click here to switch to the map view.

The map label for this gene is dnaX [C]

Identifier: 189025750

GI number: 189025750

Start: 563168

End: 564439

Strand: Reverse

Name: dnaX [C]

Synonym: TPASS_0521

Alternate gene names: 189025750

Gene position: 564439-563168 (Counterclockwise)

Preceding gene: 189025751

Following gene: 189025749

Centisome position: 49.54

GC content: 49.61

Gene sequence:

>1272_bases
GTGTTCGAGAACATTGTCGGACAGCCAGCTACTGACCTTCTGCGCGATGACGTTATGCACGCGCGTCTTCCTGCTGCGCT
CTTATTTGTTGGACCACCCGCTAGTGGCAAGCTAACTGCTGCACTTGAGCTTGCGCGCGTGCTTTCCTGTACGCAGGGGG
GCGTGTGGCAGTGTCCTTGTGCACCCTGTGTGCAGCATACGGAGTTACTTTCTCCAGAACTACTCGTGATGGGGATAAAA
GATCTGATCCTCGAAATACGCGCGTCGGCACGTGCCTATATGTCAGTGCACACGCAGGGTACGCGCTATCTTTTTGTGCG
GGCAGTGCGTAAACTCATTACACGCTTTGACGAACGACTGTGGGATAGTGATGATACTCGTTTCTCTGCCGCGGTTTCGA
GCATTGCTGAGCTCGACCAGGAGCTCGCATCACTCCCCGCACAGGGAGACCGACGTACCACTCCAGAGCAAAAAGAAAGA
GTGCAAAGGATATGCGTGATCGCCGAAAGGCTACAGCAGGAGTCACTCTATACCCAATTACCCGTACAGCAAATACGAAA
CGCAATTCAGTGGGTGCGTCTTACACCCTCAGGAAGAAAGAAAGTGCTGATCATAGAACACGCGCACGCGATGCATGAAT
CTGCACGAACTGCTTTTCTAAAGATATTAGAAGAACCTCCGCGCGATACGCTTTTTATCCTCACTACCGCTACAAAGTAT
GCAATCATGCCTACTGTCCTTTCGCGTGTGCGCAGTTATTCTTTCAGAGAAAGAAGTGTTGAACTCCAATGTGCAGTAAT
TACACGCGTATTTCATGACAGACCGACCGATGCAAAAAACACACAGGGTGTACTCTTGCACTATTTATATCAGTTTTTGT
CGTTTTCTTTAGAAGAGGTGCAAACGTCCGTGATGTACTTTTGGCTGTATGTGTGCCAGCACGCTAGGTGTATCGGACGC
ATCATTCCTGATACTTGGGTTTCGGTAGGTACTCAACCTCAAGTATCAGGAATGGATCTTACCTCACTTGATTCTACGCT
GCATTTTTTCCAAGCTCACAAACAGCAACACGCCGTGTCCCTTTTTTTCTCCTTACTCGTCAGGCACATGCGCACCCTGC
AGCGCACCACCGAGTACTCCGCACGGAATACAGAATGCTTCGCACACATTGCTCACTGTATCGAACAGGCACATCGCAAT
GTGCAGTTGTGGAATCTAACAATACAAGGGACTCTCGAACACTTAGCACACACCATCGCGAATCATCTATGA

Upstream 100 bases:

>100_bases
GTTTCGCATATGTTGCATGAAAGCGTAATTAACACTGTTCTCTCTCCCCTTGTCTTAGATGGCGTTCGCTATATGCGCCT
GAAGATGTAGGCGCGTTTTC

Downstream 100 bases:

>100_bases
GAGATTTTATTGCACGCGCGCTAAAAAAGTCAGCGAAAATGAATGACTCTCAACTGAGAAATATGATTGAACTTATTGCC
AACGAGTACACCTTGTTGGA

Product: DNA polymerase III, gamma and tau subunits

Products: diphosphate; DNAn+1

Alternate protein names: DNA Polymerase III Domain-Containing Protein; DNA Polymerase III; DNA Polymerase III Gamma And Tau Subunits; DNA Polymerase III Gamma/Tau Subunits; DNA Polymerase III Gamma/Tau Subunit; DNA Polymerase III Subunits Gamma And Tau

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MFENIVGQPATDLLRDDVMHARLPAALLFVGPPASGKLTAALELARVLSCTQGGVWQCPCAPCVQHTELLSPELLVMGIK
DLILEIRASARAYMSVHTQGTRYLFVRAVRKLITRFDERLWDSDDTRFSAAVSSIAELDQELASLPAQGDRRTTPEQKER
VQRICVIAERLQQESLYTQLPVQQIRNAIQWVRLTPSGRKKVLIIEHAHAMHESARTAFLKILEEPPRDTLFILTTATKY
AIMPTVLSRVRSYSFRERSVELQCAVITRVFHDRPTDAKNTQGVLLHYLYQFLSFSLEEVQTSVMYFWLYVCQHARCIGR
IIPDTWVSVGTQPQVSGMDLTSLDSTLHFFQAHKQQHAVSLFFSLLVRHMRTLQRTTEYSARNTECFAHIAHCIEQAHRN
VQLWNLTIQGTLEHLAHTIANHL

Sequences:

>Translated_423_residues
MFENIVGQPATDLLRDDVMHARLPAALLFVGPPASGKLTAALELARVLSCTQGGVWQCPCAPCVQHTELLSPELLVMGIK
DLILEIRASARAYMSVHTQGTRYLFVRAVRKLITRFDERLWDSDDTRFSAAVSSIAELDQELASLPAQGDRRTTPEQKER
VQRICVIAERLQQESLYTQLPVQQIRNAIQWVRLTPSGRKKVLIIEHAHAMHESARTAFLKILEEPPRDTLFILTTATKY
AIMPTVLSRVRSYSFRERSVELQCAVITRVFHDRPTDAKNTQGVLLHYLYQFLSFSLEEVQTSVMYFWLYVCQHARCIGR
IIPDTWVSVGTQPQVSGMDLTSLDSTLHFFQAHKQQHAVSLFFSLLVRHMRTLQRTTEYSARNTECFAHIAHCIEQAHRN
VQLWNLTIQGTLEHLAHTIANHL
>Mature_423_residues
MFENIVGQPATDLLRDDVMHARLPAALLFVGPPASGKLTAALELARVLSCTQGGVWQCPCAPCVQHTELLSPELLVMGIK
DLILEIRASARAYMSVHTQGTRYLFVRAVRKLITRFDERLWDSDDTRFSAAVSSIAELDQELASLPAQGDRRTTPEQKER
VQRICVIAERLQQESLYTQLPVQQIRNAIQWVRLTPSGRKKVLIIEHAHAMHESARTAFLKILEEPPRDTLFILTTATKY
AIMPTVLSRVRSYSFRERSVELQCAVITRVFHDRPTDAKNTQGVLLHYLYQFLSFSLEEVQTSVMYFWLYVCQHARCIGR
IIPDTWVSVGTQPQVSGMDLTSLDSTLHFFQAHKQQHAVSLFFSLLVRHMRTLQRTTEYSARNTECFAHIAHCIEQAHRN
VQLWNLTIQGTLEHLAHTIANHL

Specific function: DNA Polymerase III Is A Complex, Multichain Enzyme Responsible For Most Of The Replicative Synthesis In Bacteria. This DNA Polymerase Also Exhibits 3' To 5' Exonuclease Activity. The Tau Chain Serves As A Scaffold To Help In The Dimerizaton Of The Core Co

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.7

Molecular weight: Translated: 48235; Mature: 48235

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFENIVGQPATDLLRDDVMHARLPAALLFVGPPASGKLTAALELARVLSCTQGGVWQCPC
CCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEECCC
APCVQHTELLSPELLVMGIKDLILEIRASARAYMSVHTQGTRYLFVRAVRKLITRFDERL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHH
WDSDDTRFSAAVSSIAELDQELASLPAQGDRRTTPEQKERVQRICVIAERLQQESLYTQL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
PVQQIRNAIQWVRLTPSGRKKVLIIEHAHAMHESARTAFLKILEEPPRDTLFILTTATKY
CHHHHHHHHHEEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHH
AIMPTVLSRVRSYSFRERSVELQCAVITRVFHDRPTDAKNTQGVLLHYLYQFLSFSLEEV
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QTSVMYFWLYVCQHARCIGRIIPDTWVSVGTQPQVSGMDLTSLDSTLHFFQAHKQQHAVS
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LFFSLLVRHMRTLQRTTEYSARNTECFAHIAHCIEQAHRNVQLWNLTIQGTLEHLAHTIA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHH
NHL
HCC
>Mature Secondary Structure
MFENIVGQPATDLLRDDVMHARLPAALLFVGPPASGKLTAALELARVLSCTQGGVWQCPC
CCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEECCC
APCVQHTELLSPELLVMGIKDLILEIRASARAYMSVHTQGTRYLFVRAVRKLITRFDERL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHH
WDSDDTRFSAAVSSIAELDQELASLPAQGDRRTTPEQKERVQRICVIAERLQQESLYTQL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
PVQQIRNAIQWVRLTPSGRKKVLIIEHAHAMHESARTAFLKILEEPPRDTLFILTTATKY
CHHHHHHHHHEEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHH
AIMPTVLSRVRSYSFRERSVELQCAVITRVFHDRPTDAKNTQGVLLHYLYQFLSFSLEEV
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QTSVMYFWLYVCQHARCIGRIIPDTWVSVGTQPQVSGMDLTSLDSTLHFFQAHKQQHAVS
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LFFSLLVRHMRTLQRTTEYSARNTECFAHIAHCIEQAHRNVQLWNLTIQGTLEHLAHTIA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHH
NHL
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA