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The map label for this gene is ack

Identifier: 189025707

GI number: 189025707

Start: 506517

End: 507863

Strand: Reverse

Name: ack

Synonym: TPASS_0476

Alternate gene names: 189025707

Gene position: 507863-506517 (Counterclockwise)

Preceding gene: 189025708

Following gene: 189025706

Centisome position: 44.57

GC content: 52.41

Gene sequence:

>1347_bases
ATGATTATCCTCACGCTAAACTGCGGCAGTTCATCTGTAAAATACCAGGTGTATAACTGGACAGAGCGTGCGGTGATTGC
CGTCGGCGTGGTAGAGCGTGTTACTCAGGCAGGATCTGTTATCACGCATGAGGTGCACGGCCGAGAGAACCACGTTCGGG
AAAGTCCCTGCCCTACCCATACCGAAGCGGTGCAGCTTATCATCAGCACCCTCACCGATGCGCACGTCGGTGTTATCAGC
GACATGAGTCTTATCAAGGCAGTTGGACACCGCGTCCTACACGCAGCAGATCGCTTTGTAAAGTCTGTCGTTGTGACTCC
TGACGTGCTAGAAACATTTCGCTCTGTTCAAGACCTTGGCCCTCTCCACAACCCCGCAAACATCCGTGGTATTGAGGCTG
CCCAGGCGGTCATCCCCTCAGTTCCCCACTGTGCCATTATGGATACTGCGTGGCATCAAACCATGCCAGAAGCTAGCTTC
ATGTACGCAGTGCCCCGGCAGTGGTACGAAGAATACGCGGTACGTCGCTACGGCTTTCACGGCACGAGTTTTCTTTATAC
TGCAAAGCGCGCCTCTGTCATTCTCAAAAAAAGGGCTGAGGACACAAACATAATCATTGCGCATATTGGAAACGGCGCTT
CCATGTGTTGTGTCAAAAATGGTGTCAGCTTTGACACTTCCATGGGCATCACCCCGCTAGAAGGCCTGATTATGGGAACA
CGCTGCGGGGACTGCGACCCTGCGCTTGCCTTTTACATTATGAGAAAAACAGGCATGTCTGTCTCAGACATCGACACCAC
TCTCAATAAACAGTCCGGTTTGCTAGGCATCACCGGAAAATACGTGGACCGACGCGATGTGTGTGCTGCCATGAAAAATG
GAGACGCGCTTGCACGCCTTGCCTTTGACATGGAGGTACACCGCATCCGGAAATACTTCGGCGCATACGTTGCAGCTTTG
GGTAAACAGCCGGACGCGCTTGTCTTTACCGCAGGTGTTGGAGAGATGTGTTGCGACGTACGCGCAGCAGCGTGCGCAGG
ACTCGAACACCTAGGCATCCGACTTGATGCGCGAAAAAATGAACTGGCCCGCACGCGCAATGCAGAAACTGAAATTAGTA
CAGACGATTCACCCGTCCGCATTCTGGTAATCCCAACAGATGAAGAATTGGTCATGACTGAGGACACGTATGCACTCATG
CAGGGAACATACGACGTGCACACGCGTTTTACCTACTCGTTCCAGGATCCAACCTATGTCAATAAGGCGCGGGCAGCAGG
ATTAAAAAGAGATTTAGAAAAGAACCCGGCACTCGCGACGATTGTCGTGCCGCGTCTTGATACATAG

Upstream 100 bases:

>100_bases
ATATCCGCGTAAGCAGTGTAAGCCAACGGTGTAGGAAAGTGGCGCATTTTGCGTGTATGCTACGGTGTCCGAGAAATTAC
CAAAACGGTGGAGGTGTATT

Downstream 100 bases:

>100_bases
AACAGCCGGCGATATGTCTGTAGGTAAACCCTACGGGGTAGAACGATAAAAGGAGAACGCGCAGTGAATTGGAGAAATCT
TGACGAGTGTGCCGCATACG

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MIILTLNCGSSSVKYQVYNWTERAVIAVGVVERVTQAGSVITHEVHGRENHVRESPCPTHTEAVQLIISTLTDAHVGVIS
DMSLIKAVGHRVLHAADRFVKSVVVTPDVLETFRSVQDLGPLHNPANIRGIEAAQAVIPSVPHCAIMDTAWHQTMPEASF
MYAVPRQWYEEYAVRRYGFHGTSFLYTAKRASVILKKRAEDTNIIIAHIGNGASMCCVKNGVSFDTSMGITPLEGLIMGT
RCGDCDPALAFYIMRKTGMSVSDIDTTLNKQSGLLGITGKYVDRRDVCAAMKNGDALARLAFDMEVHRIRKYFGAYVAAL
GKQPDALVFTAGVGEMCCDVRAAACAGLEHLGIRLDARKNELARTRNAETEISTDDSPVRILVIPTDEELVMTEDTYALM
QGTYDVHTRFTYSFQDPTYVNKARAAGLKRDLEKNPALATIVVPRLDT

Sequences:

>Translated_448_residues
MIILTLNCGSSSVKYQVYNWTERAVIAVGVVERVTQAGSVITHEVHGRENHVRESPCPTHTEAVQLIISTLTDAHVGVIS
DMSLIKAVGHRVLHAADRFVKSVVVTPDVLETFRSVQDLGPLHNPANIRGIEAAQAVIPSVPHCAIMDTAWHQTMPEASF
MYAVPRQWYEEYAVRRYGFHGTSFLYTAKRASVILKKRAEDTNIIIAHIGNGASMCCVKNGVSFDTSMGITPLEGLIMGT
RCGDCDPALAFYIMRKTGMSVSDIDTTLNKQSGLLGITGKYVDRRDVCAAMKNGDALARLAFDMEVHRIRKYFGAYVAAL
GKQPDALVFTAGVGEMCCDVRAAACAGLEHLGIRLDARKNELARTRNAETEISTDDSPVRILVIPTDEELVMTEDTYALM
QGTYDVHTRFTYSFQDPTYVNKARAAGLKRDLEKNPALATIVVPRLDT
>Mature_448_residues
MIILTLNCGSSSVKYQVYNWTERAVIAVGVVERVTQAGSVITHEVHGRENHVRESPCPTHTEAVQLIISTLTDAHVGVIS
DMSLIKAVGHRVLHAADRFVKSVVVTPDVLETFRSVQDLGPLHNPANIRGIEAAQAVIPSVPHCAIMDTAWHQTMPEASF
MYAVPRQWYEEYAVRRYGFHGTSFLYTAKRASVILKKRAEDTNIIIAHIGNGASMCCVKNGVSFDTSMGITPLEGLIMGT
RCGDCDPALAFYIMRKTGMSVSDIDTTLNKQSGLLGITGKYVDRRDVCAAMKNGDALARLAFDMEVHRIRKYFGAYVAAL
GKQPDALVFTAGVGEMCCDVRAAACAGLEHLGIRLDARKNELARTRNAETEISTDDSPVRILVIPTDEELVMTEDTYALM
QGTYDVHTRFTYSFQDPTYVNKARAAGLKRDLEKNPALATIVVPRLDT

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=400, Percent_Identity=38, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI145693184, Length=405, Percent_Identity=36.7901234567901, Blast_Score=281, Evalue=7e-77,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_TREPA (O83489)

Other databases:

- EMBL:   AE000520
- PIR:   B71319
- RefSeq:   NP_218917.1
- ProteinModelPortal:   O83489
- SMR:   O83489
- GeneID:   2611120
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0476
- NMPDR:   fig|243276.1.peg.476
- TIGR:   TP_0476
- HOGENOM:   HBG285863
- OMA:   SSDFRDV
- ProtClustDB:   PRK00180
- BioCyc:   TPAL243276:TP_0476-MONOMER
- BRENDA:   2.7.2.1
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 49196; Mature: 49196

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIILTLNCGSSSVKYQVYNWTERAVIAVGVVERVTQAGSVITHEVHGRENHVRESPCPTH
CEEEEEECCCCCEEEEEEECCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCH
TEAVQLIISTLTDAHVGVISDMSLIKAVGHRVLHAADRFVKSVVVTPDVLETFRSVQDLG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
PLHNPANIRGIEAAQAVIPSVPHCAIMDTAWHQTMPEASFMYAVPRQWYEEYAVRRYGFH
CCCCCCCCCCHHHHHHHCCCCCCEEEEECHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCC
GTSFLYTAKRASVILKKRAEDTNIIIAHIGNGASMCCVKNGVSFDTSMGITPLEGLIMGT
CCCEEEEHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHEEEEC
RCGDCDPALAFYIMRKTGMSVSDIDTTLNKQSGLLGITGKYVDRRDVCAAMKNGDALARL
CCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCEEEHH
AFDMEVHRIRKYFGAYVAALGKQPDALVFTAGVGEMCCDVRAAACAGLEHLGIRLDARKN
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCHHHHCEEEECCHH
ELARTRNAETEISTDDSPVRILVIPTDEELVMTEDTYALMQGTYDVHTRFTYSFQDPTYV
HHHHHCCCCCEECCCCCCEEEEEEECCCCEEEECCHHHHHCCCEEEEEEEEEEECCCCHH
NKARAAGLKRDLEKNPALATIVVPRLDT
HHHHHHHHHHHHHCCCCEEEEEECCCCC
>Mature Secondary Structure
MIILTLNCGSSSVKYQVYNWTERAVIAVGVVERVTQAGSVITHEVHGRENHVRESPCPTH
CEEEEEECCCCCEEEEEEECCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCH
TEAVQLIISTLTDAHVGVISDMSLIKAVGHRVLHAADRFVKSVVVTPDVLETFRSVQDLG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
PLHNPANIRGIEAAQAVIPSVPHCAIMDTAWHQTMPEASFMYAVPRQWYEEYAVRRYGFH
CCCCCCCCCCHHHHHHHCCCCCCEEEEECHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCC
GTSFLYTAKRASVILKKRAEDTNIIIAHIGNGASMCCVKNGVSFDTSMGITPLEGLIMGT
CCCEEEEHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHEEEEC
RCGDCDPALAFYIMRKTGMSVSDIDTTLNKQSGLLGITGKYVDRRDVCAAMKNGDALARL
CCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCEEEHH
AFDMEVHRIRKYFGAYVAALGKQPDALVFTAGVGEMCCDVRAAACAGLEHLGIRLDARKN
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCHHHHCEEEECCHH
ELARTRNAETEISTDDSPVRILVIPTDEELVMTEDTYALMQGTYDVHTRFTYSFQDPTYV
HHHHHCCCCCEECCCCCCEEEEEEECCCCEEEECCHHHHHCCCEEEEEEEEEEECCCCHH
NKARAAGLKRDLEKNPALATIVVPRLDT
HHHHHHHHHHHHHCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876