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The map label for this gene is topA [H]

Identifier: 189025627

GI number: 189025627

Start: 419784

End: 421979

Strand: Direct

Name: topA [H]

Synonym: TPASS_0394

Alternate gene names: 189025627

Gene position: 419784-421979 (Clockwise)

Preceding gene: 189025626

Following gene: 189025628

Centisome position: 36.84

GC content: 47.86

Gene sequence:

>2196_bases
ATGGAAGTGCGTGGGCTACAACCGAAAAGGCAGAAGACATTTGCGCGGAAGCATTTGGTTATTGTGGAATCTCCTGCGAA
GGCGCAGACCATCGAAAAATATCTGGGAACGCAGTACGTGGTGCGAGCGTCCATGGGCCACGTTATTGACTTGCCAAAGT
CTCGTTTAGCTATTGATATTGAGCACGATTTCCAGCCTGAGTACATTACAGTTCGTGGTCGTGCGCAGTGTTTAAAAGAG
TTGCGCACCCTATCTAAGCAGTCACTGCAAGTGTTTCTTGCCAGCGATCGTGACCGAGAGGGGGAGGCGATTGCGTACCA
CCTTGCACAGTCTATCCAGGCGTATTGTGATACGCCGATCAAAAGGATCGTGTTTAATGAAATTACACCGCACGCGATTA
GGGCGGCAATTGGCCATCCGGTTCCAATAGATACCGCAAAGGTCAATGCACAAAAGGCGCGGCGCGTACTGGACCGTTTG
GTAGGATACCACCTATGTCCGCTCCTGTGGCATAAGGTGAAAAACGGGTTGTCTGCAGGACGTGTGCAGTCGGTTGCACT
TCGTCTTATCTGTGAACGAGAAGTTGAAGTAAAGCGCTTTGTTCCAGAAGAATACTGGACGGTTGAAGGAACGTTTGAAA
AGGATAAAAAGTCATTTTCTGCACTACTGATACTGATTCAAGGGAAAAAGGCTGTATTCAAAAGCAAACAGGAGGCTACA
AGCGCTATCGGTCTTTTTTCTCAGAGTGAGGCACGAGTTTCTCAAATACGCAGTTTTGAAAAAAATGTTCGACCGAAGCA
GCCCTTTACTACCTCTACGTTGCAGCAATGCGCTGCCAATAGGCTCGGGTTTACCTCGCGTAAAACGATGCAGGTTGCGC
AGCAGCTCTATGAAGGCGTATCTCTTGGTACCCACCGCGTGGGGCTCATTACATATATGCGTACAGATTCGGTGCGCGTT
TCTGAAGCTGCTGTAAAGGAAGTGCGCGCGTGGATAGCAACGCATTTTTCTGACGCACTGCCGGGTACTCCCAATCGGTA
CGCTGCAAAGGGAAAGTCTCAGGACGCACATGAGGCGATTCGACCAACATATGTTGCCCACACTCCTGAGCGGATTAAAG
CGCACCTTACGCGTGATCAAATACGTCTGTACACGTTGATTTGGGAGCGTTTTGTTGCAAGTCAGATGACAGATGCAAGA
GTGCGCAGTTTGACGTTTGAAATTACCGCTGGCCCGGCGGTTTTCAGTGCAACTGAAACGCAGGTTATTGAACAGGGATT
CTACCGTGTACTGAAAATGTTATCCCCCAAAGATCTCTCTAAAGCTGTACTTCCCCCTACGAAGGAAGGTGAGGTAGTTG
CACTACATAACGTGCAGTCCGTACAGCACTTTACGCAGGGTCCTGTTCGGTACACGGATGCAAGTATCGTAAAAATGTTG
GAGGAAAAGGGAATTGGGCGCCCTTCAACGTATGCACCGACTATTTCAGTACTGCTGGATCGCTATTATGTAACTCGTAT
TCAAAAACAGCTAATGCCGACCCCATTGGGAAAGGTCATTAGTGATCTTCTCACCACCTATTTTCATGATGTTGTTGATG
TAAGTTTTACCGCGCGCATGGAAAGTAAACTCGATGAGGTGGAGGAAGATAAAATCAAGTGGAATTGTGTCATTGCGGAT
TTCTATCCTGCTTTCAGCGAAAAGGTTTCAACGGTGATGAAAGATCTTAACAGCATGCGCGGTGTGTTCGATGAGAAAAC
AGATGTGGTGTGTAGTCAGTGTGGTGATACGATGGTGAAAAAACTGGGCAGGTTTGGATTTTTCCTTGCATGCGGAAAGT
TTCCTGAGTGTAGAAATACTCAGCCTGTCCCTCTTGCAAAGTGTCCGCGTCCTGCGTGCGATGGGAATATCGTGGGGAAA
AAAACAAGGGGAAGAAAGGAATTCTATGGCTGCACGCGTTTCCCTGTCTGCGATTTTGTCACTCACTTTAAACCGACCTC
TGCAGTGTGTCCTCAGTGTCGATGCTTTTTGGTTGAAAAATCAAATCGCAGAGTCGGCACATATACTGCGTGTGTAAACC
CTGAGTGTCGGTATGTATCTCCTACTCGAAAAAATCTTTCTCTGGGAAAGGAAGCTGTCTTGGATGGTAGCCACAGGGGG
GCACAGGATAAAGGAGCAGTGCAACATTATGAGTAA

Upstream 100 bases:

>100_bases
CACGCGCGCTGACGTGCGTGCAGGATATGTTTGACGATTGGGAATACGCATGTACGGGTCGGACGTAGGACAGTTTCGGT
AAGCATAGGGGGCGGAAAAC

Downstream 100 bases:

>100_bases
TGAGCAGTTTACCCAGTATCTTGAATATCTTTTTCGGGTACGCAGGCTGTCTGCGCATACGGTTTCTGCCTATGCGCGCG
ACTTGAATCTTTTTGAACGC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 731; Mature: 731

Protein sequence:

>731_residues
MEVRGLQPKRQKTFARKHLVIVESPAKAQTIEKYLGTQYVVRASMGHVIDLPKSRLAIDIEHDFQPEYITVRGRAQCLKE
LRTLSKQSLQVFLASDRDREGEAIAYHLAQSIQAYCDTPIKRIVFNEITPHAIRAAIGHPVPIDTAKVNAQKARRVLDRL
VGYHLCPLLWHKVKNGLSAGRVQSVALRLICEREVEVKRFVPEEYWTVEGTFEKDKKSFSALLILIQGKKAVFKSKQEAT
SAIGLFSQSEARVSQIRSFEKNVRPKQPFTTSTLQQCAANRLGFTSRKTMQVAQQLYEGVSLGTHRVGLITYMRTDSVRV
SEAAVKEVRAWIATHFSDALPGTPNRYAAKGKSQDAHEAIRPTYVAHTPERIKAHLTRDQIRLYTLIWERFVASQMTDAR
VRSLTFEITAGPAVFSATETQVIEQGFYRVLKMLSPKDLSKAVLPPTKEGEVVALHNVQSVQHFTQGPVRYTDASIVKML
EEKGIGRPSTYAPTISVLLDRYYVTRIQKQLMPTPLGKVISDLLTTYFHDVVDVSFTARMESKLDEVEEDKIKWNCVIAD
FYPAFSEKVSTVMKDLNSMRGVFDEKTDVVCSQCGDTMVKKLGRFGFFLACGKFPECRNTQPVPLAKCPRPACDGNIVGK
KTRGRKEFYGCTRFPVCDFVTHFKPTSAVCPQCRCFLVEKSNRRVGTYTACVNPECRYVSPTRKNLSLGKEAVLDGSHRG
AQDKGAVQHYE

Sequences:

>Translated_731_residues
MEVRGLQPKRQKTFARKHLVIVESPAKAQTIEKYLGTQYVVRASMGHVIDLPKSRLAIDIEHDFQPEYITVRGRAQCLKE
LRTLSKQSLQVFLASDRDREGEAIAYHLAQSIQAYCDTPIKRIVFNEITPHAIRAAIGHPVPIDTAKVNAQKARRVLDRL
VGYHLCPLLWHKVKNGLSAGRVQSVALRLICEREVEVKRFVPEEYWTVEGTFEKDKKSFSALLILIQGKKAVFKSKQEAT
SAIGLFSQSEARVSQIRSFEKNVRPKQPFTTSTLQQCAANRLGFTSRKTMQVAQQLYEGVSLGTHRVGLITYMRTDSVRV
SEAAVKEVRAWIATHFSDALPGTPNRYAAKGKSQDAHEAIRPTYVAHTPERIKAHLTRDQIRLYTLIWERFVASQMTDAR
VRSLTFEITAGPAVFSATETQVIEQGFYRVLKMLSPKDLSKAVLPPTKEGEVVALHNVQSVQHFTQGPVRYTDASIVKML
EEKGIGRPSTYAPTISVLLDRYYVTRIQKQLMPTPLGKVISDLLTTYFHDVVDVSFTARMESKLDEVEEDKIKWNCVIAD
FYPAFSEKVSTVMKDLNSMRGVFDEKTDVVCSQCGDTMVKKLGRFGFFLACGKFPECRNTQPVPLAKCPRPACDGNIVGK
KTRGRKEFYGCTRFPVCDFVTHFKPTSAVCPQCRCFLVEKSNRRVGTYTACVNPECRYVSPTRKNLSLGKEAVLDGSHRG
AQDKGAVQHYE
>Mature_731_residues
MEVRGLQPKRQKTFARKHLVIVESPAKAQTIEKYLGTQYVVRASMGHVIDLPKSRLAIDIEHDFQPEYITVRGRAQCLKE
LRTLSKQSLQVFLASDRDREGEAIAYHLAQSIQAYCDTPIKRIVFNEITPHAIRAAIGHPVPIDTAKVNAQKARRVLDRL
VGYHLCPLLWHKVKNGLSAGRVQSVALRLICEREVEVKRFVPEEYWTVEGTFEKDKKSFSALLILIQGKKAVFKSKQEAT
SAIGLFSQSEARVSQIRSFEKNVRPKQPFTTSTLQQCAANRLGFTSRKTMQVAQQLYEGVSLGTHRVGLITYMRTDSVRV
SEAAVKEVRAWIATHFSDALPGTPNRYAAKGKSQDAHEAIRPTYVAHTPERIKAHLTRDQIRLYTLIWERFVASQMTDAR
VRSLTFEITAGPAVFSATETQVIEQGFYRVLKMLSPKDLSKAVLPPTKEGEVVALHNVQSVQHFTQGPVRYTDASIVKML
EEKGIGRPSTYAPTISVLLDRYYVTRIQKQLMPTPLGKVISDLLTTYFHDVVDVSFTARMESKLDEVEEDKIKWNCVIAD
FYPAFSEKVSTVMKDLNSMRGVFDEKTDVVCSQCGDTMVKKLGRFGFFLACGKFPECRNTQPVPLAKCPRPACDGNIVGK
KTRGRKEFYGCTRFPVCDFVTHFKPTSAVCPQCRCFLVEKSNRRVGTYTACVNPECRYVSPTRKNLSLGKEAVLDGSHRG
AQDKGAVQHYE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=593, Percent_Identity=24.283305227656, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI4507635, Length=647, Percent_Identity=24.1112828438949, Blast_Score=103, Evalue=5e-22,
Organism=Escherichia coli, GI1787529, Length=767, Percent_Identity=36.6362451108214, Blast_Score=455, Evalue=1e-129,
Organism=Escherichia coli, GI1788061, Length=616, Percent_Identity=23.8636363636364, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI48994931, Length=112, Percent_Identity=33.0357142857143, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17555378, Length=493, Percent_Identity=23.9350912778905, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI32563869, Length=509, Percent_Identity=23.1827111984283, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323263, Length=492, Percent_Identity=22.5609756097561, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24640096, Length=585, Percent_Identity=23.9316239316239, Blast_Score=110, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 82382; Mature: 82382

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVRGLQPKRQKTFARKHLVIVESPAKAQTIEKYLGTQYVVRASMGHVIDLPKSRLAIDI
CCCCCCCCHHHHHHHHHCEEEEECCHHHHHHHHHHCHHHHHHHHCCCEEECCCCCEEEEE
EHDFQPEYITVRGRAQCLKELRTLSKQSLQVFLASDRDREGEAIAYHLAQSIQAYCDTPI
ECCCCCCEEEECCHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHCCHH
KRIVFNEITPHAIRAAIGHPVPIDTAKVNAQKARRVLDRLVGYHLCPLLWHKVKNGLSAG
HHHHHHCCCHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RVQSVALRLICEREVEVKRFVPEEYWTVEGTFEKDKKSFSALLILIQGKKAVFKSKQEAT
HHHHHHHHHHHHHHHHHHHCCCCHHEEECCCHHHHHHHHEEEEEEEECCHHHHHCHHHHH
SAIGLFSQSEARVSQIRSFEKNVRPKQPFTTSTLQQCAANRLGFTSRKTMQVAQQLYEGV
HHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
SLGTHRVGLITYMRTDSVRVSEAAVKEVRAWIATHFSDALPGTPNRYAAKGKSQDAHEAI
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
RPTYVAHTPERIKAHLTRDQIRLYTLIWERFVASQMTDARVRSLTFEITAGPAVFSATET
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEECHHHH
QVIEQGFYRVLKMLSPKDLSKAVLPPTKEGEVVALHNVQSVQHFTQGPVRYTDASIVKML
HHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCEEEECCHHHHHHHHCCCCEECHHHHHHHH
EEKGIGRPSTYAPTISVLLDRYYVTRIQKQLMPTPLGKVISDLLTTYFHDVVDVSFTARM
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ESKLDEVEEDKIKWNCVIADFYPAFSEKVSTVMKDLNSMRGVFDEKTDVVCSQCGDTMVK
HHHHHHHHHCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
KLGRFGFFLACGKFPECRNTQPVPLAKCPRPACDGNIVGKKTRGRKEFYGCTRFPVCDFV
HHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHH
THFKPTSAVCPQCRCFLVEKSNRRVGTYTACVNPECRYVSPTRKNLSLGKEAVLDGSHRG
HHCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCCCCHHHHHCCHHHHCCCCCCC
AQDKGAVQHYE
CCCCCCCCCCC
>Mature Secondary Structure
MEVRGLQPKRQKTFARKHLVIVESPAKAQTIEKYLGTQYVVRASMGHVIDLPKSRLAIDI
CCCCCCCCHHHHHHHHHCEEEEECCHHHHHHHHHHCHHHHHHHHCCCEEECCCCCEEEEE
EHDFQPEYITVRGRAQCLKELRTLSKQSLQVFLASDRDREGEAIAYHLAQSIQAYCDTPI
ECCCCCCEEEECCHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHCCHH
KRIVFNEITPHAIRAAIGHPVPIDTAKVNAQKARRVLDRLVGYHLCPLLWHKVKNGLSAG
HHHHHHCCCHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RVQSVALRLICEREVEVKRFVPEEYWTVEGTFEKDKKSFSALLILIQGKKAVFKSKQEAT
HHHHHHHHHHHHHHHHHHHCCCCHHEEECCCHHHHHHHHEEEEEEEECCHHHHHCHHHHH
SAIGLFSQSEARVSQIRSFEKNVRPKQPFTTSTLQQCAANRLGFTSRKTMQVAQQLYEGV
HHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
SLGTHRVGLITYMRTDSVRVSEAAVKEVRAWIATHFSDALPGTPNRYAAKGKSQDAHEAI
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
RPTYVAHTPERIKAHLTRDQIRLYTLIWERFVASQMTDARVRSLTFEITAGPAVFSATET
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEECHHHH
QVIEQGFYRVLKMLSPKDLSKAVLPPTKEGEVVALHNVQSVQHFTQGPVRYTDASIVKML
HHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCEEEECCHHHHHHHHCCCCEECHHHHHHHH
EEKGIGRPSTYAPTISVLLDRYYVTRIQKQLMPTPLGKVISDLLTTYFHDVVDVSFTARM
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ESKLDEVEEDKIKWNCVIADFYPAFSEKVSTVMKDLNSMRGVFDEKTDVVCSQCGDTMVK
HHHHHHHHHCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
KLGRFGFFLACGKFPECRNTQPVPLAKCPRPACDGNIVGKKTRGRKEFYGCTRFPVCDFV
HHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHH
THFKPTSAVCPQCRCFLVEKSNRRVGTYTACVNPECRYVSPTRKNLSLGKEAVLDGSHRG
HHCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCCCCHHHHHCCHHHHCCCCCCC
AQDKGAVQHYE
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]