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The map label for this gene is tilS

Identifier: 189025606

GI number: 189025606

Start: 397606

End: 399039

Strand: Direct

Name: tilS

Synonym: TPASS_0373

Alternate gene names: 189025606

Gene position: 397606-399039 (Clockwise)

Preceding gene: 189025605

Following gene: 189025607

Centisome position: 34.89

GC content: 56.9

Gene sequence:

>1434_bases
GTGAGTGAGTCTAGGCAGAAGCTTCACCCGCTGCTCGTCCACGTGGCGCGTAGCTTTGGGCATTTCCTTGTGCCGAGAAA
GCCTTCTTGCTTGCTCGTGGCGGTTTCAGGAGGTGCCGATTCGCTTGCGCTTCTTTATGCGGCGCACGAGCTCGCTCCTG
ACTTTGGGGTGTGTGCGTGCGCGGTCACGGTTGATCACAGTCTGCGCGCTCAGGAAGGTGCGCTCGATGCGCGTTTTGTG
CGTGCGCTGTGTGCTCGTTTTTCTCCTCCCCTTCCGTGTTTCGTGCAGCAGATTTCTGCCGGTGCGGTGCACGCGTGTGC
TAAAATTCGTGGCCGCGGTGTGCAGGACGCGGCGCGTGCACTGCGCTATAAGGTCTTTGACCACGTGGCTGCTCGCTGCG
GAGCACAGGTGGTTCTAACCGCGCACACCCGTGATGATCAGTACGAAACACTGCTCATGCGCTTATTCCAGGGAGCGGCC
GCGTCCGCGTTGCAAGGTATACGAGCTGCGCGTGGACGGTACGTGCGTCCGCTGCTAAAGGTGAGCCGCACGTGTGTTGA
AGATTTCTTGCAGACGCGTGGTGTGCGTTGGCGCGAAGACGCATCAAATACGTGCAGGAAATATGTGCGTAACCGTATCC
GTCATGAACTTATCCCTGCACTTGATGCAGTGCTTGCAGGGTGGCGCTCTGGCTTAGACAAAACGTTTGCGGGGATTAGT
GCAGAACACAGCTTTTGTGTTGCTGCCTTGACGCGGTGGCGTGAAGGTTGTTCGCATGCGTGGGAACCAGTACCACGTGC
GTTGGGCACGCGTCTGCGGATGCCTCGGTCGGACTTTCTCGCCGCTGAATTTATATTGCGCTTCTTTCTTTTGCAGGAGG
CTTGCGTTCGTTTGGGAGTTTCGCACCGGGTGCCACGCGGGGCGCTTGAACGGTGTGCGCGTTTTGATGGTGTGCGTCGG
ATACACGTTTCAGGGCTGCAGTTAGAGCGTGCCGGTGCATATGTGCTTTTTTCCTGCATACATGCGTCGGACACAGCACG
TGAGACAAAGAAACAGGATGCAGGATCACCTCCTTCCTCTGAAAAGCAAGGAGTGAGTGCAATCTACGTGGCGCGTCCTG
GTGCCTACCCCTGTGCGTGCGGAACGCTTCTTGTGGAAGTACGTCCTGCCGGCGTGTTTGTCTGTTGCGCACAGGACCAC
GTGGGTGTAGGTCCGTTTTCTTTTCCCTTTTATATCAGAACCCATCGCACGGGAGACACCATCTCCATCCGCGGTGGTCA
TAAGGGGATCCGAAAGATGTTCTCAGAGTGGCACGTACCTTTATCGGACCGCACGGTGTTACCGATGATAGAGCAGGATG
GTGTGTTGCGTGCGCTCTATGGCGCAGCACTTGGTTACCAAAATCGTTACGCGGAGAGGACTCCTCATGAGTAA

Upstream 100 bases:

>100_bases
CTTCCGAAGCGGGTTCACCCGCTGGCGCGAGGACCGGCTAGATGGTGCGCGGTGTAACGCGCGCGGTGCGGCACACTGTG
CAGCGTGCCCGTCTTTATAG

Downstream 100 bases:

>100_bases
GGGAATGTGCCCACGTATGGGAAGACTCTGTGCAGTGAGTATCTTTTGTGCATTTTCTGGCATCCAAGATGCCTCGTGCG
TGTCTGAACCTGCACCTCCA

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSESRQKLHPLLVHVARSFGHFLVPRKPSCLLVAVSGGADSLALLYAAHELAPDFGVCACAVTVDHSLRAQEGALDARFV
RALCARFSPPLPCFVQQISAGAVHACAKIRGRGVQDAARALRYKVFDHVAARCGAQVVLTAHTRDDQYETLLMRLFQGAA
ASALQGIRAARGRYVRPLLKVSRTCVEDFLQTRGVRWREDASNTCRKYVRNRIRHELIPALDAVLAGWRSGLDKTFAGIS
AEHSFCVAALTRWREGCSHAWEPVPRALGTRLRMPRSDFLAAEFILRFFLLQEACVRLGVSHRVPRGALERCARFDGVRR
IHVSGLQLERAGAYVLFSCIHASDTARETKKQDAGSPPSSEKQGVSAIYVARPGAYPCACGTLLVEVRPAGVFVCCAQDH
VGVGPFSFPFYIRTHRTGDTISIRGGHKGIRKMFSEWHVPLSDRTVLPMIEQDGVLRALYGAALGYQNRYAERTPHE

Sequences:

>Translated_477_residues
MSESRQKLHPLLVHVARSFGHFLVPRKPSCLLVAVSGGADSLALLYAAHELAPDFGVCACAVTVDHSLRAQEGALDARFV
RALCARFSPPLPCFVQQISAGAVHACAKIRGRGVQDAARALRYKVFDHVAARCGAQVVLTAHTRDDQYETLLMRLFQGAA
ASALQGIRAARGRYVRPLLKVSRTCVEDFLQTRGVRWREDASNTCRKYVRNRIRHELIPALDAVLAGWRSGLDKTFAGIS
AEHSFCVAALTRWREGCSHAWEPVPRALGTRLRMPRSDFLAAEFILRFFLLQEACVRLGVSHRVPRGALERCARFDGVRR
IHVSGLQLERAGAYVLFSCIHASDTARETKKQDAGSPPSSEKQGVSAIYVARPGAYPCACGTLLVEVRPAGVFVCCAQDH
VGVGPFSFPFYIRTHRTGDTISIRGGHKGIRKMFSEWHVPLSDRTVLPMIEQDGVLRALYGAALGYQNRYAERTPHE
>Mature_476_residues
SESRQKLHPLLVHVARSFGHFLVPRKPSCLLVAVSGGADSLALLYAAHELAPDFGVCACAVTVDHSLRAQEGALDARFVR
ALCARFSPPLPCFVQQISAGAVHACAKIRGRGVQDAARALRYKVFDHVAARCGAQVVLTAHTRDDQYETLLMRLFQGAAA
SALQGIRAARGRYVRPLLKVSRTCVEDFLQTRGVRWREDASNTCRKYVRNRIRHELIPALDAVLAGWRSGLDKTFAGISA
EHSFCVAALTRWREGCSHAWEPVPRALGTRLRMPRSDFLAAEFILRFFLLQEACVRLGVSHRVPRGALERCARFDGVRRI
HVSGLQLERAGAYVLFSCIHASDTARETKKQDAGSPPSSEKQGVSAIYVARPGAYPCACGTLLVEVRPAGVFVCCAQDHV
GVGPFSFPFYIRTHRTGDTISIRGGHKGIRKMFSEWHVPLSDRTVLPMIEQDGVLRALYGAALGYQNRYAERTPHE

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=197, Percent_Identity=30.4568527918782, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_TREPA (O83388)

Other databases:

- EMBL:   AE000520
- PIR:   D71333
- RefSeq:   NP_218813.1
- ProteinModelPortal:   O83388
- IntAct:   O83388
- GeneID:   2611257
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0373
- NMPDR:   fig|243276.1.peg.372
- TIGR:   TP_0373
- HOGENOM:   HBG731988
- OMA:   INEWNID
- ProtClustDB:   CLSK218667
- BioCyc:   TPAL243276:TP_0373-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 52606; Mature: 52475

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESRQKLHPLLVHVARSFGHFLVPRKPSCLLVAVSGGADSLALLYAAHELAPDFGVCAC
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEE
AVTVDHSLRAQEGALDARFVRALCARFSPPLPCFVQQISAGAVHACAKIRGRGVQDAARA
EEECCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHHH
LRYKVFDHVAARCGAQVVLTAHTRDDQYETLLMRLFQGAAASALQGIRAARGRYVRPLLK
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VSRTCVEDFLQTRGVRWREDASNTCRKYVRNRIRHELIPALDAVLAGWRSGLDKTFAGIS
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCC
AEHSFCVAALTRWREGCSHAWEPVPRALGTRLRMPRSDFLAAEFILRFFLLQEACVRLGV
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
SHRVPRGALERCARFDGVRRIHVSGLQLERAGAYVLFSCIHASDTARETKKQDAGSPPSS
CCCCCHHHHHHHHHHCCCEEEEECCEEEHHCCHHHHHHHHHCCHHHHHHHHHCCCCCCCC
EKQGVSAIYVARPGAYPCACGTLLVEVRPAGVFVCCAQDHVGVGPFSFPFYIRTHRTGDT
CCCCCEEEEEECCCCCCHHHHHEEEEEECCCEEEEECCCCCCCCCCCCCEEEEEECCCCE
ISIRGGHKGIRKMFSEWHVPLSDRTVLPMIEQDGVLRALYGAALGYQNRYAERTPHE
EEECCCHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SESRQKLHPLLVHVARSFGHFLVPRKPSCLLVAVSGGADSLALLYAAHELAPDFGVCAC
CCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEE
AVTVDHSLRAQEGALDARFVRALCARFSPPLPCFVQQISAGAVHACAKIRGRGVQDAARA
EEECCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHHH
LRYKVFDHVAARCGAQVVLTAHTRDDQYETLLMRLFQGAAASALQGIRAARGRYVRPLLK
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VSRTCVEDFLQTRGVRWREDASNTCRKYVRNRIRHELIPALDAVLAGWRSGLDKTFAGIS
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCC
AEHSFCVAALTRWREGCSHAWEPVPRALGTRLRMPRSDFLAAEFILRFFLLQEACVRLGV
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
SHRVPRGALERCARFDGVRRIHVSGLQLERAGAYVLFSCIHASDTARETKKQDAGSPPSS
CCCCCHHHHHHHHHHCCCEEEEECCEEEHHCCHHHHHHHHHCCHHHHHHHHHCCCCCCCC
EKQGVSAIYVARPGAYPCACGTLLVEVRPAGVFVCCAQDHVGVGPFSFPFYIRTHRTGDT
CCCCCEEEEEECCCCCCHHHHHEEEEEECCCEEEEECCCCCCCCCCCCCEEEEEECCCCE
ISIRGGHKGIRKMFSEWHVPLSDRTVLPMIEQDGVLRALYGAALGYQNRYAERTPHE
EEECCCHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876