Click here to switch to the map view.

The map label for this gene is priA [H]

Identifier: 189025463

GI number: 189025463

Start: 239422

End: 241395

Strand: Direct

Name: priA [H]

Synonym: TPASS_0230

Alternate gene names: 189025463

Gene position: 239422-241395 (Clockwise)

Preceding gene: 189025462

Following gene: 189025464

Centisome position: 21.01

GC content: 56.33

Gene sequence:

>1974_bases
ATGGCGCCGTGGCTTGAGCTTGTTTTTGACGTTCCACTGGATAAAAGCTTTACGTACCGTGCGTGTGCTGCCCACGCGGG
TGAGGCACTCGTGGGTAGACGGGTTCTTGCTCCCTTTGGGGCGCGTACACTCATTGGATTTGTGATAAGTGAATCACATT
CTTCGCCTGCTGATTGCGGTGGTGCAGTTGGCACGTTCAAGGAGATCATCCGCGTCATTGACAGGGAAGCGCTTTTTGAC
CAAACGCATCTTGCGTGTGCGCGTTGGATGGCGCATTTCTACCTGTGTGCCTTAGGTCAGGCGCTGTGTGCGGTGGTTCC
GTCTCGGAAACGAGAACGGACATTGTCTTCTTTTGCTTCTTGTGCGGGTGTTCGGCGCACTGACACCTATGCGCTTTCGG
GCGAACAGCGCAAGGCGATTGATGCGATTACCGCGAGCACCGGTGCGCGCAGTTTTTATGTGCACGGGGTGACAGGGTCG
GGGAAGACGGAAGTGTTCTTGCGCGCAGCCGAGGCAGTCCTTGCGCGTGGCAAGTCGGTTATCTATCTTGTTCCTGAGAT
AGCGCTCACTCACCAGGTGCTCCAGGAGGTATATGTGCGCTTTGGCAGTCAGGCGGCGGTGTTGCACTCAGCGCTCAGTG
GCAGTCAGCGCCTAGGTGAGTGGCGGCGCATACAGCGCATGCGTCACTGTGTAGTGATTGGAGCTCGGAGTGCAATTTTT
GCTCCGTTGAAGCGGCTGGGCCTTGTGATAATGGATGAAGAACATGACAGTTCGTATAAGTCTGCGCATGTGCCGCGCTA
TCATGCGCGGCAGGTAGCGATGTATCGCTGTGCGGACGCGAACTGTCCGTTTGTCATGGGGTCTGCAACACCGTCTGTGG
AGGCCTGGTACGCGATGCTGCGGGGGGCGGTGCGTCGTTTACCATTGACTGCGCGTGTTGCGGGGGGGGCTCCGCCGCGT
GTTGAGGTGGTGGACGTGTCAAAAGAGGCCCTGTTGCTCTCTACCCGTCTGGTGGATGAAATACGCAAGACGAAGGAGGC
AGGATATCAATCGATGCTCTTTTTGAATCGTCGAGGATTTTCCTATTCGTTTCAGTGTCGCAGCTGTGGATACACGCTGT
GTTGCACGCAGTGTGCAGTTCCCTTGACGTGGCACAAACGTGTGGGGGCAATGCAATGTCATTACTGTGGCAGGCAAGAG
GCGCCGCCTGAAAGTTGTCCGTGCTGTCATTCATTTGATACCCGATACGGCGGGGTGGGCACAGAGTATATTGAGGAAGC
AGTACAAGCGCTATTTCCTGAATACCGTATTGCACGGGTGGACACCGATGCGCTGCGCTCAGGGCACGTGCAGCAGACGA
TGGAGCAGTTTCGCGCGGGGAAAATCGATGTACTGTTGGGTACGCAAATGATAGCAAAGGGATTTAATTTCCCTACGCTG
CGTTTAGTGGGTATTGCCTGCGCAGATACTGGACTGCACACGCCAGACTTTCGCGCCGCCGAGCGGAGTTTTGCCTTGAT
GATGCAAGTGGCCGGACGTGCAGGTCGCTATGTAGATAACGGCCTGGTCATCATCCAAACACGCAATCCTGCGCATCCTG
CGGTGGTGTGTGCGCAGCACGGGGATTGTGAGTCCTTTTATGCGCAAGAACTTGCGCAGCGGGAGGCGCTGTGTTTTCCG
CCCTTTGTGCGCCTTATTCGGTTTGTTTTTCGCAGCAAGACGCGGCGCAAGGCTAAAGACGCCGCGTATGCGGCACATGC
GCTTTTGACGGCGCAGATGCCTCTGGGTGCGGATGTACTGGGACCTGCAGCGTGTGTGGTGGCGCAGGTGGCAGGCAGCT
ATCGGATGCAAATACTGCTGCGTGCCCCATCATTCCCAGTGGTGCAGCAGGTGGCGCGCAGCTTTTTAGATGAATTTCGA
GCTCCGGCGGGGGTGTACGTAGAATCTGACGTAGATCCTGTAAATGTACTGTAG

Upstream 100 bases:

>100_bases
CGGGATGAAGCGCTGAAACGTCCGGCGTGGGAGGATCTCAAAACGTTTCGTGCACGGTTGCTGCAGTTGAAGCAGGACGC
ACACATGCCAATATAAAATC

Downstream 100 bases:

>100_bases
GGCGAGTAGATGTACTCCGTGTTATCCTGCTGTTTGCGTGTTTGGTTGACCGGTAGTATGCGGTGCCTGGTATAGGTGCG
GGACGGAAAGGAGAGAGGAT

Product: primosomal protein N

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 657; Mature: 656

Protein sequence:

>657_residues
MAPWLELVFDVPLDKSFTYRACAAHAGEALVGRRVLAPFGARTLIGFVISESHSSPADCGGAVGTFKEIIRVIDREALFD
QTHLACARWMAHFYLCALGQALCAVVPSRKRERTLSSFASCAGVRRTDTYALSGEQRKAIDAITASTGARSFYVHGVTGS
GKTEVFLRAAEAVLARGKSVIYLVPEIALTHQVLQEVYVRFGSQAAVLHSALSGSQRLGEWRRIQRMRHCVVIGARSAIF
APLKRLGLVIMDEEHDSSYKSAHVPRYHARQVAMYRCADANCPFVMGSATPSVEAWYAMLRGAVRRLPLTARVAGGAPPR
VEVVDVSKEALLLSTRLVDEIRKTKEAGYQSMLFLNRRGFSYSFQCRSCGYTLCCTQCAVPLTWHKRVGAMQCHYCGRQE
APPESCPCCHSFDTRYGGVGTEYIEEAVQALFPEYRIARVDTDALRSGHVQQTMEQFRAGKIDVLLGTQMIAKGFNFPTL
RLVGIACADTGLHTPDFRAAERSFALMMQVAGRAGRYVDNGLVIIQTRNPAHPAVVCAQHGDCESFYAQELAQREALCFP
PFVRLIRFVFRSKTRRKAKDAAYAAHALLTAQMPLGADVLGPAACVVAQVAGSYRMQILLRAPSFPVVQQVARSFLDEFR
APAGVYVESDVDPVNVL

Sequences:

>Translated_657_residues
MAPWLELVFDVPLDKSFTYRACAAHAGEALVGRRVLAPFGARTLIGFVISESHSSPADCGGAVGTFKEIIRVIDREALFD
QTHLACARWMAHFYLCALGQALCAVVPSRKRERTLSSFASCAGVRRTDTYALSGEQRKAIDAITASTGARSFYVHGVTGS
GKTEVFLRAAEAVLARGKSVIYLVPEIALTHQVLQEVYVRFGSQAAVLHSALSGSQRLGEWRRIQRMRHCVVIGARSAIF
APLKRLGLVIMDEEHDSSYKSAHVPRYHARQVAMYRCADANCPFVMGSATPSVEAWYAMLRGAVRRLPLTARVAGGAPPR
VEVVDVSKEALLLSTRLVDEIRKTKEAGYQSMLFLNRRGFSYSFQCRSCGYTLCCTQCAVPLTWHKRVGAMQCHYCGRQE
APPESCPCCHSFDTRYGGVGTEYIEEAVQALFPEYRIARVDTDALRSGHVQQTMEQFRAGKIDVLLGTQMIAKGFNFPTL
RLVGIACADTGLHTPDFRAAERSFALMMQVAGRAGRYVDNGLVIIQTRNPAHPAVVCAQHGDCESFYAQELAQREALCFP
PFVRLIRFVFRSKTRRKAKDAAYAAHALLTAQMPLGADVLGPAACVVAQVAGSYRMQILLRAPSFPVVQQVARSFLDEFR
APAGVYVESDVDPVNVL
>Mature_656_residues
APWLELVFDVPLDKSFTYRACAAHAGEALVGRRVLAPFGARTLIGFVISESHSSPADCGGAVGTFKEIIRVIDREALFDQ
THLACARWMAHFYLCALGQALCAVVPSRKRERTLSSFASCAGVRRTDTYALSGEQRKAIDAITASTGARSFYVHGVTGSG
KTEVFLRAAEAVLARGKSVIYLVPEIALTHQVLQEVYVRFGSQAAVLHSALSGSQRLGEWRRIQRMRHCVVIGARSAIFA
PLKRLGLVIMDEEHDSSYKSAHVPRYHARQVAMYRCADANCPFVMGSATPSVEAWYAMLRGAVRRLPLTARVAGGAPPRV
EVVDVSKEALLLSTRLVDEIRKTKEAGYQSMLFLNRRGFSYSFQCRSCGYTLCCTQCAVPLTWHKRVGAMQCHYCGRQEA
PPESCPCCHSFDTRYGGVGTEYIEEAVQALFPEYRIARVDTDALRSGHVQQTMEQFRAGKIDVLLGTQMIAKGFNFPTLR
LVGIACADTGLHTPDFRAAERSFALMMQVAGRAGRYVDNGLVIIQTRNPAHPAVVCAQHGDCESFYAQELAQREALCFPP
FVRLIRFVFRSKTRRKAKDAAYAAHALLTAQMPLGADVLGPAACVVAQVAGSYRMQILLRAPSFPVVQQVARSFLDEFRA
PAGVYVESDVDPVNVL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=738, Percent_Identity=31.5718157181572, Blast_Score=337, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 72222; Mature: 72091

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPWLELVFDVPLDKSFTYRACAAHAGEALVGRRVLAPFGARTLIGFVISESHSSPADCG
CCCHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCC
GAVGTFKEIIRVIDREALFDQTHLACARWMAHFYLCALGQALCAVVPSRKRERTLSSFAS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
CAGVRRTDTYALSGEQRKAIDAITASTGARSFYVHGVTGSGKTEVFLRAAEAVLARGKSV
HCCCCCCCCEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEE
IYLVPEIALTHQVLQEVYVRFGSQAAVLHSALSGSQRLGEWRRIQRMRHCVVIGARSAIF
EEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHEEEECHHHHH
APLKRLGLVIMDEEHDSSYKSAHVPRYHARQVAMYRCADANCPFVMGSATPSVEAWYAML
HHHHHCCEEEEECCCCCCCHHCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHH
RGAVRRLPLTARVAGGAPPRVEVVDVSKEALLLSTRLVDEIRKTKEAGYQSMLFLNRRGF
HHHHHHCCCEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHEEEEECCCC
SYSFQCRSCGYTLCCTQCAVPLTWHKRVGAMQCHYCGRQEAPPESCPCCHSFDTRYGGVG
CEEEEECCCCCEEEHHHHCCCCHHHHHCCCEEHHCCCCCCCCCCCCCCCCCCCCCCCCCC
TEYIEEAVQALFPEYRIARVDTDALRSGHVQQTMEQFRAGKIDVLLGTQMIAKGFNFPTL
HHHHHHHHHHHCCCCEEEECCHHHHHCCHHHHHHHHHCCCCEEEEECHHHHHCCCCCCCE
RLVGIACADTGLHTPDFRAAERSFALMMQVAGRAGRYVDNGLVIIQTRNPAHPAVVCAQH
EEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCEEEEEECCCCCCEEEEECC
GDCESFYAQELAQREALCFPPFVRLIRFVFRSKTRRKAKDAAYAAHALLTAQMPLGADVL
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
GPAACVVAQVAGSYRMQILLRAPSFPVVQQVARSFLDEFRAPAGVYVESDVDPVNVL
HHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
>Mature Secondary Structure 
APWLELVFDVPLDKSFTYRACAAHAGEALVGRRVLAPFGARTLIGFVISESHSSPADCG
CCHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCC
GAVGTFKEIIRVIDREALFDQTHLACARWMAHFYLCALGQALCAVVPSRKRERTLSSFAS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
CAGVRRTDTYALSGEQRKAIDAITASTGARSFYVHGVTGSGKTEVFLRAAEAVLARGKSV
HCCCCCCCCEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEE
IYLVPEIALTHQVLQEVYVRFGSQAAVLHSALSGSQRLGEWRRIQRMRHCVVIGARSAIF
EEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHEEEECHHHHH
APLKRLGLVIMDEEHDSSYKSAHVPRYHARQVAMYRCADANCPFVMGSATPSVEAWYAML
HHHHHCCEEEEECCCCCCCHHCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHH
RGAVRRLPLTARVAGGAPPRVEVVDVSKEALLLSTRLVDEIRKTKEAGYQSMLFLNRRGF
HHHHHHCCCEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHEEEEECCCC
SYSFQCRSCGYTLCCTQCAVPLTWHKRVGAMQCHYCGRQEAPPESCPCCHSFDTRYGGVG
CEEEEECCCCCEEEHHHHCCCCHHHHHCCCEEHHCCCCCCCCCCCCCCCCCCCCCCCCCC
TEYIEEAVQALFPEYRIARVDTDALRSGHVQQTMEQFRAGKIDVLLGTQMIAKGFNFPTL
HHHHHHHHHHHCCCCEEEECCHHHHHCCHHHHHHHHHCCCCEEEEECHHHHHCCCCCCCE
RLVGIACADTGLHTPDFRAAERSFALMMQVAGRAGRYVDNGLVIIQTRNPAHPAVVCAQH
EEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCEEEEEECCCCCCEEEEECC
GDCESFYAQELAQREALCFPPFVRLIRFVFRSKTRRKAKDAAYAAHALLTAQMPLGADVL
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
GPAACVVAQVAGSYRMQILLRAPSFPVVQQVARSFLDEFRAPAGVYVESDVDPVNVL
HHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]