Click here to switch to the map view.

The map label for this gene is engD [H]

Identifier: 189025358

GI number: 189025358

Start: 145568

End: 146674

Strand: Direct

Name: engD [H]

Synonym: TPASS_0124

Alternate gene names: 189025358

Gene position: 145568-146674 (Clockwise)

Preceding gene: 189025357

Following gene: 189025359

Centisome position: 12.78

GC content: 52.57

Gene sequence:

>1107_bases
ATGGCGGTCAATTGTGGCATTATCGGTCTGCCGAATGTGGGGAAGTCGACAATTTTCTCCGCGCTCACTGCAAACGTCGT
GGAGGCGGCGAATTATCCCTTTTGTACTATCGAACCTAACGTGGGTATGGTGACAGTACCTGATGTGCGTCTTGAAGCAC
TGGCTGGTCATTTTCGGCCAAAGAAAACGGTGTATGCCTCCATTGAATGTGTGGATATTGCTGGTTTGGTAAAAGGTGCC
TCGCAGGGGGAGGGATTGGGCAATCGTTTTCTTGCGCATGTGCGAGAGGTTGGAGTACTTGCACATGTGGTGCGCTGTTT
TGAGCATACGGATATCGTTCATGTACATAATAAGGTCGATCCTCTTTCAGATATTGAAACGGTGCATATAGAGCTGGCAT
TGGCAGACCTGGCCTCGGTAGAAAAACGGGCTGTGCGTGCTCAAAAGGAGTCGCGTATGGGAAAGTCCCTTCAAAAGGAA
AGCACGCTGGTATTACGGGCACTCGATACGCTGCGCGAATATTTAGAAATGGGAAAGGCGGCATGTATGGCGCCGCTGTC
GGATGAGGAGCGCAACGCGGTGCGCGATATGCGCTTGTTGACAATGAAGCCGCACCTGTACGTGTGCAATACAGACGAAA
GCGGCATGCAGTACGGAAATGATTTCGTGCGCGCGGTGCAAGAGCACGCACGTGTGCATAACACGCAGGCAATTGTTATG
TGTGGAAAATTTGAAGCAGAGCTTGCGCAGCTTTCTGATGTGGCAGAGCAAAACGCCTTTTTGCAAGAATTAGGGTTGCG
CGAATCAGGACGTGCGGCGCTTGCGCGCGCAGTGTATTCCCTGATGGGGTTGCGTACCTTTTTTACCGCGGGGCCTGAGG
AGTGTCGCGCGTGGACCATTCGGGCAGGGCTGCGTGCACCGCACGCGGCAGGAGTGATCCACAGCGACCTTGAGCGTGGT
TTTATTCGTGCAGAAACGTATTCTTTCGATGATCTTGCGTCCTGTGGGAGTGTGGCAAAGGTGAGGGAGGCAAACCGCGT
TCGGCAGGAGGGGAAGGAATACGAGGTGCAAGACGGGGACGTTATCTTTTTTAAATTCAATGTGTGA

Upstream 100 bases:

>100_bases
GAAAATACCGTTCGCGCACAGTTCATGCAGGATAACTTTTGGAATGCAAAACTGTTTGCCGCCGCGCAGGCAAACAAACT
CATTTAAAGCAGGGGGCACT

Downstream 100 bases:

>100_bases
AACACAGGCGCTCCGTTCCGTCTGTGCGCCGTGTGCGATACAGTGAGCCTTGATTCTGCGTTTGAAAGCAGGCACAATGC
GTCCCGTGCAGCGTATCATA

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MAVNCGIIGLPNVGKSTIFSALTANVVEAANYPFCTIEPNVGMVTVPDVRLEALAGHFRPKKTVYASIECVDIAGLVKGA
SQGEGLGNRFLAHVREVGVLAHVVRCFEHTDIVHVHNKVDPLSDIETVHIELALADLASVEKRAVRAQKESRMGKSLQKE
STLVLRALDTLREYLEMGKAACMAPLSDEERNAVRDMRLLTMKPHLYVCNTDESGMQYGNDFVRAVQEHARVHNTQAIVM
CGKFEAELAQLSDVAEQNAFLQELGLRESGRAALARAVYSLMGLRTFFTAGPEECRAWTIRAGLRAPHAAGVIHSDLERG
FIRAETYSFDDLASCGSVAKVREANRVRQEGKEYEVQDGDVIFFKFNV

Sequences:

>Translated_368_residues
MAVNCGIIGLPNVGKSTIFSALTANVVEAANYPFCTIEPNVGMVTVPDVRLEALAGHFRPKKTVYASIECVDIAGLVKGA
SQGEGLGNRFLAHVREVGVLAHVVRCFEHTDIVHVHNKVDPLSDIETVHIELALADLASVEKRAVRAQKESRMGKSLQKE
STLVLRALDTLREYLEMGKAACMAPLSDEERNAVRDMRLLTMKPHLYVCNTDESGMQYGNDFVRAVQEHARVHNTQAIVM
CGKFEAELAQLSDVAEQNAFLQELGLRESGRAALARAVYSLMGLRTFFTAGPEECRAWTIRAGLRAPHAAGVIHSDLERG
FIRAETYSFDDLASCGSVAKVREANRVRQEGKEYEVQDGDVIFFKFNV
>Mature_367_residues
AVNCGIIGLPNVGKSTIFSALTANVVEAANYPFCTIEPNVGMVTVPDVRLEALAGHFRPKKTVYASIECVDIAGLVKGAS
QGEGLGNRFLAHVREVGVLAHVVRCFEHTDIVHVHNKVDPLSDIETVHIELALADLASVEKRAVRAQKESRMGKSLQKES
TLVLRALDTLREYLEMGKAACMAPLSDEERNAVRDMRLLTMKPHLYVCNTDESGMQYGNDFVRAVQEHARVHNTQAIVMC
GKFEAELAQLSDVAEQNAFLQELGLRESGRAALARAVYSLMGLRTFFTAGPEECRAWTIRAGLRAPHAAGVIHSDLERGF
IRAETYSFDDLASCGSVAKVREANRVRQEGKEYEVQDGDVIFFKFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=379, Percent_Identity=39.3139841688654, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI58761502, Length=186, Percent_Identity=38.1720430107527, Blast_Score=135, Evalue=5e-32,
Organism=Escherichia coli, GI1787454, Length=368, Percent_Identity=48.3695652173913, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI1789574, Length=130, Percent_Identity=32.3076923076923, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17509631, Length=368, Percent_Identity=38.0434782608696, Blast_Score=253, Evalue=9e-68,
Organism=Saccharomyces cerevisiae, GI6319499, Length=375, Percent_Identity=36, Blast_Score=222, Evalue=9e-59,
Organism=Saccharomyces cerevisiae, GI6321773, Length=382, Percent_Identity=34.5549738219895, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6321649, Length=194, Percent_Identity=31.4432989690722, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24640873, Length=370, Percent_Identity=38.3783783783784, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24640877, Length=370, Percent_Identity=38.3783783783784, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24640875, Length=370, Percent_Identity=38.3783783783784, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24640879, Length=329, Percent_Identity=36.4741641337386, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24585318, Length=145, Percent_Identity=30.3448275862069, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 40491; Mature: 40360

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVNCGIIGLPNVGKSTIFSALTANVVEAANYPFCTIEPNVGMVTVPDVRLEALAGHFRP
CEEECCEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCHHHHHHHHCCCC
KKTVYASIECVDIAGLVKGASQGEGLGNRFLAHVREVGVLAHVVRCFEHTDIVHVHNKVD
CCEEEEEEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
PLSDIETVHIELALADLASVEKRAVRAQKESRMGKSLQKESTLVLRALDTLREYLEMGKA
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
ACMAPLSDEERNAVRDMRLLTMKPHLYVCNTDESGMQYGNDFVRAVQEHARVHNTQAIVM
HHCCCCCCHHHHHHHHHHEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEE
CGKFEAELAQLSDVAEQNAFLQELGLRESGRAALARAVYSLMGLRTFFTAGPEECRAWTI
ECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHEEEE
RAGLRAPHAAGVIHSDLERGFIRAETYSFDDLASCGSVAKVREANRVRQEGKEYEVQDGD
ECCCCCCCHHHHHHHHHHCCEEEEECCCCHHHHHCCHHHHHHHHHHHHHCCCCEEECCCC
VIFFKFNV
EEEEEECC
>Mature Secondary Structure 
AVNCGIIGLPNVGKSTIFSALTANVVEAANYPFCTIEPNVGMVTVPDVRLEALAGHFRP
EEECCEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCHHHHHHHHCCCC
KKTVYASIECVDIAGLVKGASQGEGLGNRFLAHVREVGVLAHVVRCFEHTDIVHVHNKVD
CCEEEEEEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
PLSDIETVHIELALADLASVEKRAVRAQKESRMGKSLQKESTLVLRALDTLREYLEMGKA
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
ACMAPLSDEERNAVRDMRLLTMKPHLYVCNTDESGMQYGNDFVRAVQEHARVHNTQAIVM
HHCCCCCCHHHHHHHHHHEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEE
CGKFEAELAQLSDVAEQNAFLQELGLRESGRAALARAVYSLMGLRTFFTAGPEECRAWTI
ECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHEEEE
RAGLRAPHAAGVIHSDLERGFIRAETYSFDDLASCGSVAKVREANRVRQEGKEYEVQDGD
ECCCCCCCHHHHHHHHHHCCEEEEECCCCHHHHHCCHHHHHHHHHHHHHCCCCEEECCCC
VIFFKFNV
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]