Click here to switch to the map view.

The map label for this gene is exoA [H]

Identifier: 189025359

GI number: 189025359

Start: 146751

End: 147545

Strand: Direct

Name: exoA [H]

Synonym: TPASS_0125

Alternate gene names: 189025359

Gene position: 146751-147545 (Clockwise)

Preceding gene: 189025358

Following gene: 189025361

Centisome position: 12.88

GC content: 56.6

Gene sequence:

>795_bases
ATGCGTCCCGTGCAGCGTATCATATCTTGGAATGTGAATGGAATTCGTGCCATAGAGCGGAAAGATTTTCTCAGCTGGCT
CGCGCGTGAGGCGCCTGATGTTCTCTGTTTGCAGGAGATTAAAGCGCATGAGTCGCAGCTGAGTGCTGCGCTTCGTGCTC
CGGTCTGGAGTGCTGGGGCGGGGGGTACGTACTATACCTATTTTCACAGTGCGCAGCGTCCTGGATACAGTGGCACGGCG
CTGTTCAGTAAGCGCGCGCCAGATGCGGTGCGTTTCTTCGGGGTTCCGGCTTTTGACTGCGAGGGGCGGATGCTTGCGGC
ACGCTTTGGCGAGCTGACGGTGGTAAGCGCGTATTTTCCGAATGCGCAGGAAGGGGGCAAGCGGCTCGCGTATAAGCTTG
ATTTTTGCGCAGCGTTTCGTGCGTTCTGTGATGAAGAGCGTACGGCCGGGCAGCACGTGATCTTGTGTGGTGACTACAAC
ATAGCGCATAAGGAAATCGACCTGGCACATCCTCAGGAAAATGAGGGGAATCCTGGATTCCTGCCTCAGGAGCGTGCATG
GATGGATACATTTACGGAGGCAGGCTATGCGGATAGCTTCCGAGCCTTCTGCACAGAAGGGCAGCAGTACACGTGGTGGA
GCTACCGTGCCCGTGCACGCGCGCGTAACATTGGATGGCGCATCGATTACCAGTGTGTGGACCAAGCCTTTTTAGCGCGC
GTGACCTCTTCGCAGATACTGTCCGAGGTGACAGGATCGGATCACTGCCCAGTGTGTTTGACGTACGCGGACTAA

Upstream 100 bases:

>100_bases
ATCTTTTTTAAATTCAATGTGTGAAACACAGGCGCTCCGTTCCGTCTGTGCGCCGTGTGCGATACAGTGAGCCTTGATTC
TGCGTTTGAAAGCAGGCACA

Downstream 100 bases:

>100_bases
TCCGTTTCCGGGGTGAGCGGCACGTCCGCGCAAACTAAGACGTACCCGCGCGCACAGGCAGCGTCAGAGGTGGTAGCGAA
CGTCCACACCCGCGGCTATG

Product: exodeoxyribonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRPVQRIISWNVNGIRAIERKDFLSWLAREAPDVLCLQEIKAHESQLSAALRAPVWSAGAGGTYYTYFHSAQRPGYSGTA
LFSKRAPDAVRFFGVPAFDCEGRMLAARFGELTVVSAYFPNAQEGGKRLAYKLDFCAAFRAFCDEERTAGQHVILCGDYN
IAHKEIDLAHPQENEGNPGFLPQERAWMDTFTEAGYADSFRAFCTEGQQYTWWSYRARARARNIGWRIDYQCVDQAFLAR
VTSSQILSEVTGSDHCPVCLTYAD

Sequences:

>Translated_264_residues
MRPVQRIISWNVNGIRAIERKDFLSWLAREAPDVLCLQEIKAHESQLSAALRAPVWSAGAGGTYYTYFHSAQRPGYSGTA
LFSKRAPDAVRFFGVPAFDCEGRMLAARFGELTVVSAYFPNAQEGGKRLAYKLDFCAAFRAFCDEERTAGQHVILCGDYN
IAHKEIDLAHPQENEGNPGFLPQERAWMDTFTEAGYADSFRAFCTEGQQYTWWSYRARARARNIGWRIDYQCVDQAFLAR
VTSSQILSEVTGSDHCPVCLTYAD
>Mature_264_residues
MRPVQRIISWNVNGIRAIERKDFLSWLAREAPDVLCLQEIKAHESQLSAALRAPVWSAGAGGTYYTYFHSAQRPGYSGTA
LFSKRAPDAVRFFGVPAFDCEGRMLAARFGELTVVSAYFPNAQEGGKRLAYKLDFCAAFRAFCDEERTAGQHVILCGDYN
IAHKEIDLAHPQENEGNPGFLPQERAWMDTFTEAGYADSFRAFCTEGQQYTWWSYRARARARNIGWRIDYQCVDQAFLAR
VTSSQILSEVTGSDHCPVCLTYAD

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=262, Percent_Identity=40.4580152671756, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI18375503, Length=262, Percent_Identity=40.4580152671756, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI18375501, Length=262, Percent_Identity=40.4580152671756, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI18375507, Length=321, Percent_Identity=29.595015576324, Blast_Score=99, Evalue=3e-21,
Organism=Escherichia coli, GI1788046, Length=276, Percent_Identity=29.7101449275362, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71989536, Length=266, Percent_Identity=35.3383458646617, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI221330655, Length=258, Percent_Identity=36.4341085271318, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI17136678, Length=258, Percent_Identity=36.4341085271318, Blast_Score=166, Evalue=2e-41,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29743; Mature: 29743

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPVQRIISWNVNGIRAIERKDFLSWLAREAPDVLCLQEIKAHESQLSAALRAPVWSAGA
CCHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
GGTYYTYFHSAQRPGYSGTALFSKRAPDAVRFFGVPAFDCEGRMLAARFGELTVVSAYFP
CCEEEEEEECCCCCCCCCCEEECCCCCCCEEEECCCCCCCCCCEEEECCCCEEEEEEECC
NAQEGGKRLAYKLDFCAAFRAFCDEERTAGQHVILCGDYNIAHKEIDLAHPQENEGNPGF
CCHHCCCEEEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCCCCCCCCCCC
LPQERAWMDTFTEAGYADSFRAFCTEGQQYTWWSYRARARARNIGWRIDYQCVDQAFLAR
CCCHHHHHHHHHHCCCCHHHHHHHCCCCEEEEHHHHHHHHHHCCCEEECHHHHHHHHHHH
VTSSQILSEVTGSDHCPVCLTYAD
HHHHHHHHHHCCCCCCCEEEEECC
>Mature Secondary Structure
MRPVQRIISWNVNGIRAIERKDFLSWLAREAPDVLCLQEIKAHESQLSAALRAPVWSAGA
CCHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
GGTYYTYFHSAQRPGYSGTALFSKRAPDAVRFFGVPAFDCEGRMLAARFGELTVVSAYFP
CCEEEEEEECCCCCCCCCCEEECCCCCCCEEEECCCCCCCCCCEEEECCCCEEEEEEECC
NAQEGGKRLAYKLDFCAAFRAFCDEERTAGQHVILCGDYNIAHKEIDLAHPQENEGNPGF
CCHHCCCEEEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCCCCCCCCCCC
LPQERAWMDTFTEAGYADSFRAFCTEGQQYTWWSYRARARARNIGWRIDYQCVDQAFLAR
CCCHHHHHHHHHHCCCCHHHHHHHCCCCEEEEHHHHHHHHHHCCCEEECHHHHHHHHHHH
VTSSQILSEVTGSDHCPVCLTYAD
HHHHHHHHHHCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]