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The map label for this gene is rlmB [H]

Identifier: 189025288

GI number: 189025288

Start: 62961

End: 63920

Strand: Direct

Name: rlmB [H]

Synonym: TPASS_0054

Alternate gene names: 189025288

Gene position: 62961-63920 (Clockwise)

Preceding gene: 189025286

Following gene: 189025289

Centisome position: 5.53

GC content: 53.12

Gene sequence:

>960_bases
ATGCTCGAGCACATGAAGCGTGAACAAGCACGAAGTCAGCTATCACACGAGCCTCCTAAGCGGCGCCGAGCCTCTCTAAC
CGTCTGCGGCCTGCGTGCTGTGGAAACGCTTGGCAGCATACATCCAGAGAAAATTCATCGGTTCTTTTTTACCCCTGTGC
GTGCGAAACGCTTCGGACCCCTGTGTGCATACCTTGCTGCACGAAAGAGGCTCTACCGTAGCGCTAATACGCAGGAACTT
GAGCGATTGACGCAATCCGTTCACCATCAGGGGGTTGCTGCTACCATAGACGAGCCGCGCTTTCCAGCCGTGACTCATTC
TCAGGTTGAATTTTGGGTACAACGGCGTGAGTTTGTTGTGTTACTCGATCGCGTAGGAGATGCCCACAATCTGGGGGCGA
TTATACGTAGTGCTGCTTTTTTTGGAGTGCACTCACTGGTGGTGAGTGACTGTCGACAGCAGGCGCAGGTTACAAGCGCA
ACATATCGGGTTGCGCAGGGAGGAATGGAGTTTGTGCAATTGTTGCGCTGTACAAATGCGCAGGAAGTATTGGAAATGTG
TGCAGGTAAAATGACCCGTGTGGGAGCCTCCCCTCATGCGTTCAGATCGCTTACACGGCTTTCAAACATACTCTCGCCTG
AAGAAGCGGTAATATTAGTACTGGGAAACGAGGAGACAGGGCTTTCTGAGCATTTGACTGCGCATTGCGATCATCTCTGT
CGGATTGCAGGCAGTGGTCAGGTGGAAAGTCTAAATGTTGCGCAAGCGGGTGCGCTTTTTTTGTCCACTATCGTACAGTT
GCGTCAATCTCCTCAGGACTACACGCAGGGACATCGGGCCACGCCACGTGCACAAGAGCGTGTGCACCGCTGTGGGCAAT
TAGAGGAAAAGGGGCAGAAAAATGGAGCACGTGTTCTTATTCCCCGCTCGGGGGCGCGTGCCAATTCCCGTGAAAGTTGA

Upstream 100 bases:

>100_bases
GGCGTAAAAATGTCTGTCTGCAGGGCTCTGTGTCTTGTGTGGTGGTCCCTGTTTTGTGGGTAGGTTTGGGGAAACCTAAC
AGAGACGTAGGAAATCCGTT

Downstream 100 bases:

>100_bases
GAGTAGGGAAAGTAGACGGGTGTGAGGTATGGAACCTACAGCGCAATTGGTTTTGCAGTGCGTGTTGACGTGCGTCCGCG
TGTGCCTCTGAGAACGCAGC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MLEHMKREQARSQLSHEPPKRRRASLTVCGLRAVETLGSIHPEKIHRFFFTPVRAKRFGPLCAYLAARKRLYRSANTQEL
ERLTQSVHHQGVAATIDEPRFPAVTHSQVEFWVQRREFVVLLDRVGDAHNLGAIIRSAAFFGVHSLVVSDCRQQAQVTSA
TYRVAQGGMEFVQLLRCTNAQEVLEMCAGKMTRVGASPHAFRSLTRLSNILSPEEAVILVLGNEETGLSEHLTAHCDHLC
RIAGSGQVESLNVAQAGALFLSTIVQLRQSPQDYTQGHRATPRAQERVHRCGQLEEKGQKNGARVLIPRSGARANSRES

Sequences:

>Translated_319_residues
MLEHMKREQARSQLSHEPPKRRRASLTVCGLRAVETLGSIHPEKIHRFFFTPVRAKRFGPLCAYLAARKRLYRSANTQEL
ERLTQSVHHQGVAATIDEPRFPAVTHSQVEFWVQRREFVVLLDRVGDAHNLGAIIRSAAFFGVHSLVVSDCRQQAQVTSA
TYRVAQGGMEFVQLLRCTNAQEVLEMCAGKMTRVGASPHAFRSLTRLSNILSPEEAVILVLGNEETGLSEHLTAHCDHLC
RIAGSGQVESLNVAQAGALFLSTIVQLRQSPQDYTQGHRATPRAQERVHRCGQLEEKGQKNGARVLIPRSGARANSRES
>Mature_319_residues
MLEHMKREQARSQLSHEPPKRRRASLTVCGLRAVETLGSIHPEKIHRFFFTPVRAKRFGPLCAYLAARKRLYRSANTQEL
ERLTQSVHHQGVAATIDEPRFPAVTHSQVEFWVQRREFVVLLDRVGDAHNLGAIIRSAAFFGVHSLVVSDCRQQAQVTSA
TYRVAQGGMEFVQLLRCTNAQEVLEMCAGKMTRVGASPHAFRSLTRLSNILSPEEAVILVLGNEETGLSEHLTAHCDHLC
RIAGSGQVESLNVAQAGALFLSTIVQLRQSPQDYTQGHRATPRAQERVHRCGQLEEKGQKNGARVLIPRSGARANSRES

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=254, Percent_Identity=31.496062992126, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788935, Length=258, Percent_Identity=31.0077519379845, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 35537; Mature: 35537

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEHMKREQARSQLSHEPPKRRRASLTVCGLRAVETLGSIHPEKIHRFFFTPVRAKRFGP
CCHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHH
LCAYLAARKRLYRSANTQELERLTQSVHHQGVAATIDEPRFPAVTHSQVEFWVQRREFVV
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHH
LLDRVGDAHNLGAIIRSAAFFGVHSLVVSDCRQQAQVTSATYRVAQGGMEFVQLLRCTNA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCH
QEVLEMCAGKMTRVGASPHAFRSLTRLSNILSPEEAVILVLGNEETGLSEHLTAHCDHLC
HHHHHHHHCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
RIAGSGQVESLNVAQAGALFLSTIVQLRQSPQDYTQGHRATPRAQERVHRCGQLEEKGQK
HHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHCCHHHHHCC
NGARVLIPRSGARANSRES
CCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MLEHMKREQARSQLSHEPPKRRRASLTVCGLRAVETLGSIHPEKIHRFFFTPVRAKRFGP
CCHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHH
LCAYLAARKRLYRSANTQELERLTQSVHHQGVAATIDEPRFPAVTHSQVEFWVQRREFVV
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHH
LLDRVGDAHNLGAIIRSAAFFGVHSLVVSDCRQQAQVTSATYRVAQGGMEFVQLLRCTNA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCH
QEVLEMCAGKMTRVGASPHAFRSLTRLSNILSPEEAVILVLGNEETGLSEHLTAHCDHLC
HHHHHHHHCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
RIAGSGQVESLNVAQAGALFLSTIVQLRQSPQDYTQGHRATPRAQERVHRCGQLEEKGQK
HHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHCCHHHHHCC
NGARVLIPRSGARANSRES
CCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]