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The map label for this gene is greA

Identifier: 189025253

GI number: 189025253

Start: 21555

End: 22043

Strand: Direct

Name: greA

Synonym: TPASS_0019

Alternate gene names: 189025253

Gene position: 21555-22043 (Clockwise)

Preceding gene: 189025252

Following gene: 189025254

Centisome position: 1.89

GC content: 45.4

Gene sequence:

>489_bases
GTGGTTTCCGGACGTGGACTAGTGGTAACTGCAAAGATGCTCAATGCAAAAAAGAAAGAATTGCAGGATTTGCTTGATGT
TCGTATTCCGGAAAATTCTCGAGAGATTGGTAGGGCCTTAGAACTCGGTGATTTGCGTGAGAACGCAGAGTATAAGGCTG
CGCGAGAAGAACAAACAAGGTTGAACAATATGGTGACTCGGCTACAAGAGGAGATTGAGCGGGCACAGGTATTCGATCCT
ACCACTGTTGTAGCTGGCAGAGTTTCGTTTGGTACGGTAATTAGCTTAAAAAATCACACAAGTGGAGAAGATGAGACATA
CACTATTCTTGGTCCGTGGGAGTCGGCTCCAGAACGTGGTATTATTTCGTACATGTCTCCGTTAGGTAGCAATCTGCTCA
ATCGTAAGACAGGGGAACAACTTGCCTTTACGGTGGGAGAACATGAAAAGGTGTATGAGATCTTAAGCATCTCTGCTGCA
GAGATCTAG

Upstream 100 bases:

>100_bases
AAGTGATATCCAGGGGCTTGATCCAAAGTTAAAGGTCCATTTGCGCAATAAAATTATTGAGAAGTACAAGGATTTTAAGT
TTTTTGATACTGAGGAACGT

Downstream 100 bases:

>100_bases
TGAGGAAGTGTGCGATGCGAATTATGCGGAGATTAATGTTATTTCTTATGTGTCTATGTGCTGCGCTGTTTGCGCAAGAG
CTGGTTCGCGAACAGAGTGT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MVSGRGLVVTAKMLNAKKKELQDLLDVRIPENSREIGRALELGDLRENAEYKAAREEQTRLNNMVTRLQEEIERAQVFDP
TTVVAGRVSFGTVISLKNHTSGEDETYTILGPWESAPERGIISYMSPLGSNLLNRKTGEQLAFTVGEHEKVYEILSISAA
EI

Sequences:

>Translated_162_residues
MVSGRGLVVTAKMLNAKKKELQDLLDVRIPENSREIGRALELGDLRENAEYKAAREEQTRLNNMVTRLQEEIERAQVFDP
TTVVAGRVSFGTVISLKNHTSGEDETYTILGPWESAPERGIISYMSPLGSNLLNRKTGEQLAFTVGEHEKVYEILSISAA
EI
>Mature_162_residues
MVSGRGLVVTAKMLNAKKKELQDLLDVRIPENSREIGRALELGDLRENAEYKAAREEQTRLNNMVTRLQEEIERAQVFDP
TTVVAGRVSFGTVISLKNHTSGEDETYTILGPWESAPERGIISYMSPLGSNLLNRKTGEQLAFTVGEHEKVYEILSISAA
EI

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=141, Percent_Identity=34.0425531914894, Blast_Score=78, Evalue=3e-16,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_TREPA (O83063)

Other databases:

- EMBL:   AE000520
- PIR:   G71375
- RefSeq:   NP_218459.1
- ProteinModelPortal:   O83063
- SMR:   O83063
- IntAct:   O83063
- GeneID:   2610785
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0019
- NMPDR:   fig|243276.1.peg.18
- TIGR:   TP_0019
- HOGENOM:   HBG753387
- OMA:   TPGGFVK
- ProtClustDB:   CLSK2300108
- BioCyc:   TPAL243276:TP_0019-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 18047; Mature: 18047

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSGRGLVVTAKMLNAKKKELQDLLDVRIPENSREIGRALELGDLRENAEYKAAREEQTR
CCCCCCEEEEHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LNNMVTRLQEEIERAQVFDPTTVVAGRVSFGTVISLKNHTSGEDETYTILGPWESAPERG
HHHHHHHHHHHHHHHHCCCCHHHEECCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCH
IISYMSPLGSNLLNRKTGEQLAFTVGEHEKVYEILSISAAEI
HHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVSGRGLVVTAKMLNAKKKELQDLLDVRIPENSREIGRALELGDLRENAEYKAAREEQTR
CCCCCCEEEEHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LNNMVTRLQEEIERAQVFDPTTVVAGRVSFGTVISLKNHTSGEDETYTILGPWESAPERG
HHHHHHHHHHHHHHHHCCCCHHHEECCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCH
IISYMSPLGSNLLNRKTGEQLAFTVGEHEKVYEILSISAAEI
HHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876