Click here to switch to the map view.

The map label for this gene is purA

Identifier: 188587498

GI number: 188587498

Start: 3143713

End: 3145002

Strand: Reverse

Name: purA

Synonym: Nther_2909

Alternate gene names: 188587498

Gene position: 3145002-3143713 (Counterclockwise)

Preceding gene: 188587499

Following gene: 188587497

Centisome position: 99.35

GC content: 38.76

Gene sequence:

>1290_bases
ATGTCAAGTGTTATAGTGGTAGGAAGCCAATGGGGAGACGAAGGTAAAGGAAAGGTTACGGACTATCTAGCCGAAGATTC
AGGTGTCATCGTTCGCTATCAAGGTGGCAATAATGCAGGACATACTGTTAGTATTGGAGATAAAGTTTATAAATTACATT
TGATTCCCTCTGGGATTTTCCATGGTAACAAAACTTGTGTTCTAGGGAATGGTATGGTAATAGACCCCAAAAGCCTGATT
GAGGAACTAGAATACTTGAAAAATCATGGTATTACTTCCTTTGAATTGATTATTAGTGACCGGGCTCATGTGATAATGCC
ATATCATAAAGTCATCGATGAAATGGAGGAACAGGAAAAAGGAGATAAGAAAATTGGTACTACTGGTAAAGGAATTGGCC
CATGCTATATGGATAAAGCTTCAAGAGTAGGAGTGAGAATGGGCGACCTAGTTGATGAAGAAGAATTAGAGGACAAAGTG
CGCTGGGCCGTTGAGAAAAAAAATGAGCTACTAAAGGATGTATATGGAGCGGACGGTTTTGACTCAGAATCTGTAATTCA
AGAGTATAAGAAATATGCCGAGAAACTAAAGCCATTTATTAAAGATGCATCAGTATTTTTAGAGCAAGCATATAATAGGG
GTGAAGAGATCCTTTTTGAAGGTGCACAAGGAACTCTACTGGATTTAGACCATGGTACTTATCCTTACGTGACAGCTTCG
AATCCTGTAACAGGTGGAGCTTGCGTTGGCGCTGGAGCACCACCCCACCTTATCAACAGTGTAGTAGGTGTGGTCAAAGC
ATACTCTACCAGAGTTGGTGACGGTCCTTTTCCCAGTGAACTGACAGGTGAAATTGGAGATCAAATTAGGGAAAAAGGCA
AGGAGTACGGAACTACTACTGGAAGACCTCGTAGAGTTGGCTGGCTTGACACAGTAATATTAAATTACTCCAGAAGAATT
AACGGTTTTAACCATATTGCATTGACATTGCTTGATGTTTTATCAGAATTTGATACAATAAAGATCTGTGTTGCATATAA
ATATAGAGGAGAAACAATTGAACATTTTCCAGCAAGTCAAAAAATATTATCGGAATGTGAACCGGTATTTGAAGAACTAC
CTGGTTGGAAAGAAGATATATCTCAGATTCAGGACTGGGATGAATTACCCGATGCAGCTAAATCTTATATTAAAAGAATA
GAAGATCTGACCTCTGTACCAGTCTCTATAGTTTCTTATGGTCCCCGGCGATCTCAAACTAAGATAAGAAATAAAATAAA
AAATAAATAA

Upstream 100 bases:

>100_bases
AACTAAAACCCGTTAAAAGTGAGATTTATTGTATAAAATTGATTTGATAGAGTTATTAATAGATTATACCAATAAAAGGT
ATGAGAGGTGGGGTAATTTA

Downstream 100 bases:

>100_bases
AGGGTTGACAGATTAAGCTGAGTAGTGTACCATTAGTAAATGTCAGAGGCGTGAGCCATTAGCTCAGTTGGCAGAGCACC
GGACTTTTAATCCGGGTGTC

Product: Adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSSVIVVGSQWGDEGKGKVTDYLAEDSGVIVRYQGGNNAGHTVSIGDKVYKLHLIPSGIFHGNKTCVLGNGMVIDPKSLI
EELEYLKNHGITSFELIISDRAHVIMPYHKVIDEMEEQEKGDKKIGTTGKGIGPCYMDKASRVGVRMGDLVDEEELEDKV
RWAVEKKNELLKDVYGADGFDSESVIQEYKKYAEKLKPFIKDASVFLEQAYNRGEEILFEGAQGTLLDLDHGTYPYVTAS
NPVTGGACVGAGAPPHLINSVVGVVKAYSTRVGDGPFPSELTGEIGDQIREKGKEYGTTTGRPRRVGWLDTVILNYSRRI
NGFNHIALTLLDVLSEFDTIKICVAYKYRGETIEHFPASQKILSECEPVFEELPGWKEDISQIQDWDELPDAAKSYIKRI
EDLTSVPVSIVSYGPRRSQTKIRNKIKNK

Sequences:

>Translated_429_residues
MSSVIVVGSQWGDEGKGKVTDYLAEDSGVIVRYQGGNNAGHTVSIGDKVYKLHLIPSGIFHGNKTCVLGNGMVIDPKSLI
EELEYLKNHGITSFELIISDRAHVIMPYHKVIDEMEEQEKGDKKIGTTGKGIGPCYMDKASRVGVRMGDLVDEEELEDKV
RWAVEKKNELLKDVYGADGFDSESVIQEYKKYAEKLKPFIKDASVFLEQAYNRGEEILFEGAQGTLLDLDHGTYPYVTAS
NPVTGGACVGAGAPPHLINSVVGVVKAYSTRVGDGPFPSELTGEIGDQIREKGKEYGTTTGRPRRVGWLDTVILNYSRRI
NGFNHIALTLLDVLSEFDTIKICVAYKYRGETIEHFPASQKILSECEPVFEELPGWKEDISQIQDWDELPDAAKSYIKRI
EDLTSVPVSIVSYGPRRSQTKIRNKIKNK
>Mature_428_residues
SSVIVVGSQWGDEGKGKVTDYLAEDSGVIVRYQGGNNAGHTVSIGDKVYKLHLIPSGIFHGNKTCVLGNGMVIDPKSLIE
ELEYLKNHGITSFELIISDRAHVIMPYHKVIDEMEEQEKGDKKIGTTGKGIGPCYMDKASRVGVRMGDLVDEEELEDKVR
WAVEKKNELLKDVYGADGFDSESVIQEYKKYAEKLKPFIKDASVFLEQAYNRGEEILFEGAQGTLLDLDHGTYPYVTASN
PVTGGACVGAGAPPHLINSVVGVVKAYSTRVGDGPFPSELTGEIGDQIREKGKEYGTTTGRPRRVGWLDTVILNYSRRIN
GFNHIALTLLDVLSEFDTIKICVAYKYRGETIEHFPASQKILSECEPVFEELPGWKEDISQIQDWDELPDAAKSYIKRIE
DLTSVPVSIVSYGPRRSQTKIRNKIKNK

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI34577063, Length=422, Percent_Identity=47.1563981042654, Blast_Score=380, Evalue=1e-106,
Organism=Homo sapiens, GI22748717, Length=420, Percent_Identity=44.5238095238095, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI40316944, Length=391, Percent_Identity=42.9667519181586, Blast_Score=325, Evalue=5e-89,
Organism=Escherichia coli, GI1790620, Length=423, Percent_Identity=46.3356973995272, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25146553, Length=430, Percent_Identity=41.1627906976744, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI25146555, Length=430, Percent_Identity=41.1627906976744, Blast_Score=330, Evalue=1e-90,
Organism=Saccharomyces cerevisiae, GI6324109, Length=435, Percent_Identity=44.1379310344828, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI21356033, Length=417, Percent_Identity=45.5635491606715, Blast_Score=369, Evalue=1e-102,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_NATTJ (B2A449)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001919043.1
- ProteinModelPortal:   B2A449
- SMR:   B2A449
- GeneID:   6315025
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_2909
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47613; Mature: 47481

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVIVVGSQWGDEGKGKVTDYLAEDSGVIVRYQGGNNAGHTVSIGDKVYKLHLIPSGIF
CCCEEEEECCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCEEEECCEEEEEEEECCCCC
HGNKTCVLGNGMVIDPKSLIEELEYLKNHGITSFELIISDRAHVIMPYHKVIDEMEEQEK
CCCCEEEECCCEEECHHHHHHHHHHHHHCCCCEEEEEECCCCCEEECHHHHHHHHHHHHC
GDKKIGTTGKGIGPCYMDKASRVGVRMGDLVDEEELEDKVRWAVEKKNELLKDVYGADGF
CCCCCCCCCCCCCCHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DSESVIQEYKKYAEKLKPFIKDASVFLEQAYNRGEEILFEGAQGTLLDLDHGTYPYVTAS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCEEECC
NPVTGGACVGAGAPPHLINSVVGVVKAYSTRVGDGPFPSELTGEIGDQIREKGKEYGTTT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
GRPRRVGWLDTVILNYSRRINGFNHIALTLLDVLSEFDTIKICVAYKYRGETIEHFPASQ
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCHHHCCCHH
KILSECEPVFEELPGWKEDISQIQDWDELPDAAKSYIKRIEDLTSVPVSIVSYGPRRSQT
HHHHHHHHHHHHCCCHHHHHHHHHCHHHCHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHH
KIRNKIKNK
HHHHHHCCC
>Mature Secondary Structure 
SSVIVVGSQWGDEGKGKVTDYLAEDSGVIVRYQGGNNAGHTVSIGDKVYKLHLIPSGIF
CCEEEEECCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCEEEECCEEEEEEEECCCCC
HGNKTCVLGNGMVIDPKSLIEELEYLKNHGITSFELIISDRAHVIMPYHKVIDEMEEQEK
CCCCEEEECCCEEECHHHHHHHHHHHHHCCCCEEEEEECCCCCEEECHHHHHHHHHHHHC
GDKKIGTTGKGIGPCYMDKASRVGVRMGDLVDEEELEDKVRWAVEKKNELLKDVYGADGF
CCCCCCCCCCCCCCHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DSESVIQEYKKYAEKLKPFIKDASVFLEQAYNRGEEILFEGAQGTLLDLDHGTYPYVTAS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCEEECC
NPVTGGACVGAGAPPHLINSVVGVVKAYSTRVGDGPFPSELTGEIGDQIREKGKEYGTTT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
GRPRRVGWLDTVILNYSRRINGFNHIALTLLDVLSEFDTIKICVAYKYRGETIEHFPASQ
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCHHHCCCHH
KILSECEPVFEELPGWKEDISQIQDWDELPDAAKSYIKRIEDLTSVPVSIVSYGPRRSQT
HHHHHHHHHHHHCCCHHHHHHHHHCHHHCHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHH
KIRNKIKNK
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA