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The map label for this gene is rutD [H]

Identifier: 188587327

GI number: 188587327

Start: 2949060

End: 2949875

Strand: Reverse

Name: rutD [H]

Synonym: Nther_2732

Alternate gene names: 188587327

Gene position: 2949875-2949060 (Counterclockwise)

Preceding gene: 188587328

Following gene: 188587326

Centisome position: 93.19

GC content: 42.03

Gene sequence:

>816_bases
TTGAAAGGAGGGGTGATTATTCCTACAGTTAAAGCCAATGGTATTAATATATATTATGAAGTAAAAGGTGAGGGGACTCC
TCTCTTGTTGATAGAAGGCTTGGGTTATGCTACCTGGATGTGGTATCGACAGGAGAAATTACAACAGAATTTTCAACTAA
TCAAATTTGACAATAGAGGGGTTGGGGGTTCAGATAAACCCCAAGATCCCTATTCCGTTGAATTGATGGCCCAGGATGCA
GCTCAGCTAATGGAAGCCCTTGAAATTGACAAAGCCAATGTTTTAGGGGTTTCTCTAGGCGGGTTCATTGCCCAAGAACT
GGCGTATAGCTATCCTGACAAGGTAGAGAAGTTGATTCTTTGTTCAACATCCTTTGGTGGGCCCAATAGTATTCCGATTC
CCCAGGAAACCTTAGAAGTTATGCAAAAAGGTGGGGGTGAATACTCTTCCCTGGATGAATTAAGAAATGCCGTGGGTGTT
GCTCTAGACCGTCGGTTATGGGACGAAAACCAAGATATAATAGATAAAATTTTACACGAGAAATCTACAAATCCCCAGCC
AAAACACGCATATCTGAGACAAATGGAAGCTGGCGCAGCTTTTAATGGTGAAGAAAAAACTTCCCATATAAAGGCACCCA
CTCTTATATTAGCAGCTAAAGGTGACCGGGTAGTTCCCTGGGAAAATGCTCAGTTGCTGCACGATAAAATTCCACATTCT
CGCCTGGAACTATTATCAGAAGGTGGGCACTTGTTTTTTATGGAGAGACCTGACACAGCTAATAATTTAATTCGGGAGTT
TTTGAAGGAGGATTAA

Upstream 100 bases:

>100_bases
TTTGAACAGGTAACTGATTTCAGAGAATTCGGAGAATAATATTTTACTTACCTTGCTAGTGTACTATTAGTAGATGAGAA
ATATATGAGAAGTCTATCCC

Downstream 100 bases:

>100_bases
TCAATGAATAGCTTAGTACAAAATGCCGTGATTACAGGTACAGGCAAGTATCTTCCTCCACATCTGTACACTAATAATGA
TATTGCAGAAATATTAGGCC

Product: alpha/beta hydrolase fold

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: Aminohydrolase [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKGGVIIPTVKANGINIYYEVKGEGTPLLLIEGLGYATWMWYRQEKLQQNFQLIKFDNRGVGGSDKPQDPYSVELMAQDA
AQLMEALEIDKANVLGVSLGGFIAQELAYSYPDKVEKLILCSTSFGGPNSIPIPQETLEVMQKGGGEYSSLDELRNAVGV
ALDRRLWDENQDIIDKILHEKSTNPQPKHAYLRQMEAGAAFNGEEKTSHIKAPTLILAAKGDRVVPWENAQLLHDKIPHS
RLELLSEGGHLFFMERPDTANNLIREFLKED

Sequences:

>Translated_271_residues
MKGGVIIPTVKANGINIYYEVKGEGTPLLLIEGLGYATWMWYRQEKLQQNFQLIKFDNRGVGGSDKPQDPYSVELMAQDA
AQLMEALEIDKANVLGVSLGGFIAQELAYSYPDKVEKLILCSTSFGGPNSIPIPQETLEVMQKGGGEYSSLDELRNAVGV
ALDRRLWDENQDIIDKILHEKSTNPQPKHAYLRQMEAGAAFNGEEKTSHIKAPTLILAAKGDRVVPWENAQLLHDKIPHS
RLELLSEGGHLFFMERPDTANNLIREFLKED
>Mature_271_residues
MKGGVIIPTVKANGINIYYEVKGEGTPLLLIEGLGYATWMWYRQEKLQQNFQLIKFDNRGVGGSDKPQDPYSVELMAQDA
AQLMEALEIDKANVLGVSLGGFIAQELAYSYPDKVEKLILCSTSFGGPNSIPIPQETLEVMQKGGGEYSSLDELRNAVGV
ALDRRLWDENQDIIDKILHEKSTNPQPKHAYLRQMEAGAAFNGEEKTSHIKAPTLILAAKGDRVVPWENAQLLHDKIPHS
RLELLSEGGHLFFMERPDTANNLIREFLKED

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR019913 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 30251; Mature: 30251

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGGVIIPTVKANGINIYYEVKGEGTPLLLIEGLGYATWMWYRQEKLQQNFQLIKFDNRG
CCCCEEEEEEECCCEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCC
VGGSDKPQDPYSVELMAQDAAQLMEALEIDKANVLGVSLGGFIAQELAYSYPDKVEKLIL
CCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCHHHHEEEE
CSTSFGGPNSIPIPQETLEVMQKGGGEYSSLDELRNAVGVALDRRLWDENQDIIDKILHE
EECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KSTNPQPKHAYLRQMEAGAAFNGEEKTSHIKAPTLILAAKGDRVVPWENAQLLHDKIPHS
CCCCCCHHHHHHHHHHCCCCCCCCCHHCCCCCCEEEEEECCCEECCCCCCHHHHHHCCHH
RLELLSEGGHLFFMERPDTANNLIREFLKED
HHHHHHCCCCEEEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKGGVIIPTVKANGINIYYEVKGEGTPLLLIEGLGYATWMWYRQEKLQQNFQLIKFDNRG
CCCCEEEEEEECCCEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCC
VGGSDKPQDPYSVELMAQDAAQLMEALEIDKANVLGVSLGGFIAQELAYSYPDKVEKLIL
CCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCHHHHEEEE
CSTSFGGPNSIPIPQETLEVMQKGGGEYSSLDELRNAVGVALDRRLWDENQDIIDKILHE
EECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KSTNPQPKHAYLRQMEAGAAFNGEEKTSHIKAPTLILAAKGDRVVPWENAQLLHDKIPHS
CCCCCCHHHHHHHHHHCCCCCCCCCHHCCCCCCEEEEEECCCEECCCCCCHHHHHHCCHH
RLELLSEGGHLFFMERPDTANNLIREFLKED
HHHHHHCCCCEEEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA