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The map label for this gene is livJ [C]

Identifier: 188587328

GI number: 188587328

Start: 2949937

End: 2951160

Strand: Reverse

Name: livJ [C]

Synonym: Nther_2733

Alternate gene names: 188587328

Gene position: 2951160-2949937 (Counterclockwise)

Preceding gene: 188587329

Following gene: 188587327

Centisome position: 93.23

GC content: 42.81

Gene sequence:

>1224_bases
ATGTTAACAGTTTGTAACAGAGCTGTACTGCCAATCGCTATTTTGGCGATACTGATGGTAGCTGTGATGACAGGTTGTGA
AGAAGAGGTTGGAAATGGAGAACTTTCTCAAGGAGTAACGGAAGACGAGATTTTAGTGGGCAGTGTTGGTCCACAATCAG
GGCCCTTTGCTTTTATGGGTAGCCCTTACTACGCTGGTATGGAATCCTATTTCAAGACAATAAACGAAGAGGGAGGCGTG
GATGGACGAGAAATCAACCTAAAAGTTCAGGACGATGAATTTGAACCAGATAACGCTATTTCAGGCATGGAGACTTTAAT
TTATGAAGAGGAAGTCTTTGCTATAGTTGGTCAGCTTGGAACTCCTGGGATCCTGGCAACTATGGATATGGTAAGAGAAG
AGGGAATTCCCTCGGTTTACTTTGGAGGAGGTGCTTCTGAATTTAGAGAAGCAGGTGAAAACTTTTTTCCTGTTCAACCT
ATTTACGATTATGAAGGTAAACTTATGGCAGAATACGCTATAGATGAGTTTGATGCAGATGAATTGGTTGTTATTTATGC
AAATGACGATGTGGGCCAAGATGGACTACAGGGAATCAAAACTGGTCTTGAGGAAATGGGACGAGAAGATGCCCTAGATG
CAGATAGAGAGATCTCCTTTAACCCTGGAGATACGGACTTTTCCAGTCAAATTCAATTAGCAAGAGATCAGGATCCGGAT
ATGGTATTACTATATGCCCTGTCAGACGATGCGGCTAATATATTGGGTGATTTTGAGGACGCCTCTTACGAGGTTCCCAT
AATGACCACATATTCTAACACGGATGATTCATTTTTAGAAATGGCAGCCCCTCAAGCTCCTCAAGTAATGACAGAACTTT
ATTCCCTGGGCTGGTTAGAGATGGATGAACAGGCCCTTCAACCTCTAAATGACGCAATGGAAGAATATTACCCTGACGAA
CCTATTAATGCTTATACTATGGCCGGTTGGGTAGCAGCTGAAACCTTTGTGGCAGGTCTTGAAGAAGCAGGAGATGACTT
GACTTGGGACGGTTATATTGATGCTATGAATCAGCTTCACTTTACAGAAGGTCTGGCACCTGAAATCTCTTATGAGCCCG
GAGTCCGTGAAGGTGTAACTCATATGTCTGTCAGCGAAGTGGTTGAAGAAAATGGTGAGTATAGATTTGAACAGGTAACT
GATTTCAGAGAATTCGGAGAATAA

Upstream 100 bases:

>100_bases
TATGAAATTTTCTTCCTAGTCCTTAAAATGGATGGTAGTGAAATCTTGAGAATGAGTGAGTATATTGAAGAAATAATTAA
TTTATAGAGGGAGGGACATT

Downstream 100 bases:

>100_bases
TATTTTACTTACCTTGCTAGTGTACTATTAGTAGATGAGAAATATATGAGAAGTCTATCCCTTGAAAGGAGGGGTGATTA
TTCCTACAGTTAAAGCCAAT

Product: Extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MLTVCNRAVLPIAILAILMVAVMTGCEEEVGNGELSQGVTEDEILVGSVGPQSGPFAFMGSPYYAGMESYFKTINEEGGV
DGREINLKVQDDEFEPDNAISGMETLIYEEEVFAIVGQLGTPGILATMDMVREEGIPSVYFGGGASEFREAGENFFPVQP
IYDYEGKLMAEYAIDEFDADELVVIYANDDVGQDGLQGIKTGLEEMGREDALDADREISFNPGDTDFSSQIQLARDQDPD
MVLLYALSDDAANILGDFEDASYEVPIMTTYSNTDDSFLEMAAPQAPQVMTELYSLGWLEMDEQALQPLNDAMEEYYPDE
PINAYTMAGWVAAETFVAGLEEAGDDLTWDGYIDAMNQLHFTEGLAPEISYEPGVREGVTHMSVSEVVEENGEYRFEQVT
DFREFGE

Sequences:

>Translated_407_residues
MLTVCNRAVLPIAILAILMVAVMTGCEEEVGNGELSQGVTEDEILVGSVGPQSGPFAFMGSPYYAGMESYFKTINEEGGV
DGREINLKVQDDEFEPDNAISGMETLIYEEEVFAIVGQLGTPGILATMDMVREEGIPSVYFGGGASEFREAGENFFPVQP
IYDYEGKLMAEYAIDEFDADELVVIYANDDVGQDGLQGIKTGLEEMGREDALDADREISFNPGDTDFSSQIQLARDQDPD
MVLLYALSDDAANILGDFEDASYEVPIMTTYSNTDDSFLEMAAPQAPQVMTELYSLGWLEMDEQALQPLNDAMEEYYPDE
PINAYTMAGWVAAETFVAGLEEAGDDLTWDGYIDAMNQLHFTEGLAPEISYEPGVREGVTHMSVSEVVEENGEYRFEQVT
DFREFGE
>Mature_407_residues
MLTVCNRAVLPIAILAILMVAVMTGCEEEVGNGELSQGVTEDEILVGSVGPQSGPFAFMGSPYYAGMESYFKTINEEGGV
DGREINLKVQDDEFEPDNAISGMETLIYEEEVFAIVGQLGTPGILATMDMVREEGIPSVYFGGGASEFREAGENFFPVQP
IYDYEGKLMAEYAIDEFDADELVVIYANDDVGQDGLQGIKTGLEEMGREDALDADREISFNPGDTDFSSQIQLARDQDPD
MVLLYALSDDAANILGDFEDASYEVPIMTTYSNTDDSFLEMAAPQAPQVMTELYSLGWLEMDEQALQPLNDAMEEYYPDE
PINAYTMAGWVAAETFVAGLEEAGDDLTWDGYIDAMNQLHFTEGLAPEISYEPGVREGVTHMSVSEVVEENGEYRFEQVT
DFREFGE

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=337, Percent_Identity=20.7715133531157, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 44804; Mature: 44804

Theoretical pI: Translated: 3.39; Mature: 3.39

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTVCNRAVLPIAILAILMVAVMTGCEEEVGNGELSQGVTEDEILVGSVGPQSGPFAFMG
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCHHHCCCCCCCEEEECCCCCCCCEEEEC
SPYYAGMESYFKTINEEGGVDGREINLKVQDDEFEPDNAISGMETLIYEEEVFAIVGQLG
CCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
TPGILATMDMVREEGIPSVYFGGGASEFREAGENFFPVQPIYDYEGKLMAEYAIDEFDAD
CCHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCCCCCEECCCCCEEEEHHHCCCCCC
ELVVIYANDDVGQDGLQGIKTGLEEMGREDALDADREISFNPGDTDFSSQIQLARDQDPD
CEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHHHEEECCCCCCC
MVLLYALSDDAANILGDFEDASYEVPIMTTYSNTDDSFLEMAAPQAPQVMTELYSLGWLE
EEEEEEECCCHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHCCCCC
MDEQALQPLNDAMEEYYPDEPINAYTMAGWVAAETFVAGLEEAGDDLTWDGYIDAMNQLH
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
FTEGLAPEISYEPGVREGVTHMSVSEVVEENGEYRFEQVTDFREFGE
HHCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCCEEHHHHHHHHHHCC
>Mature Secondary Structure
MLTVCNRAVLPIAILAILMVAVMTGCEEEVGNGELSQGVTEDEILVGSVGPQSGPFAFMG
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCHHHCCCCCCCEEEECCCCCCCCEEEEC
SPYYAGMESYFKTINEEGGVDGREINLKVQDDEFEPDNAISGMETLIYEEEVFAIVGQLG
CCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
TPGILATMDMVREEGIPSVYFGGGASEFREAGENFFPVQPIYDYEGKLMAEYAIDEFDAD
CCHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCCCCCEECCCCCEEEEHHHCCCCCC
ELVVIYANDDVGQDGLQGIKTGLEEMGREDALDADREISFNPGDTDFSSQIQLARDQDPD
CEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHHHEEECCCCCCC
MVLLYALSDDAANILGDFEDASYEVPIMTTYSNTDDSFLEMAAPQAPQVMTELYSLGWLE
EEEEEEECCCHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHCCCCC
MDEQALQPLNDAMEEYYPDEPINAYTMAGWVAAETFVAGLEEAGDDLTWDGYIDAMNQLH
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
FTEGLAPEISYEPGVREGVTHMSVSEVVEENGEYRFEQVTDFREFGE
HHCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCCEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]