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The map label for this gene is nadB [C]

Identifier: 188587263

GI number: 188587263

Start: 2869851

End: 2871467

Strand: Reverse

Name: nadB [C]

Synonym: Nther_2665

Alternate gene names: 188587263

Gene position: 2871467-2869851 (Counterclockwise)

Preceding gene: 188587264

Following gene: 188587262

Centisome position: 90.71

GC content: 42.73

Gene sequence:

>1617_bases
ATGAGTTATACACCTGAAATGAGAGAATTGATAAAAAAAGTTGAAGAGACTAGAGATCAGAGGATAGGAAATTACTTCCC
CAGGATGACCCCGGAGGAGCGGGAAGAGGTTTTGAATAATAATCATCCCGACTATAAGCAGGACCAATTTAGAGATGCTA
AAATTGGCCCAAATAAAGGTGATAAATTTCCTCACGAGCTGTGTGACCTGATTGAGGCCTATCCTTCTATAGATTGGAAT
CAGTTTACAACTGATCAAATAGATTATGATGTTGATGTTTTAGTTATCGGAGGTGGAGGAGCAGGCGCATCAGCTTCTCT
ACTAGCTCATGAAGCCGGAGCTGACGTACTCATGGCAACTAAACTTCGTTTTGGTGATGCCAATACGGTGATGGCTCAAG
GAGGCATTCAAGCTGCAGATAAATCAAATGATTCTCCATCAACTCATTACTTAGATGTAATAGGTGGAGGACATTATTAC
AATGTTCCAGAACTGGTTAGAGCTCTAGTCTCTGATGCTCCGGATGTAATTAGCTGGCTCGAGGACTTGGGCGTTATGTT
TGATAAAGATGATGAAGGTACAATGCAAACCAAACACGGTGGCGGTACATCCAGAAAACGAATGCATTCGGCTAGAGACT
ACACCGGGGCTGAAATTATGAGGGTACTAAGAGACGAAGTACATTGCCGCGATATTAATGTAGTGGAATTCAGCCCAGCT
GTTGAATTATTGCTTGACAAACAGGGGAGAGCATCAGGAGCCATACTTTACAACATGGAAACAAAAGAGTATCAAGTCGT
TAAAGCAAAAACAGTTATCATCACTACAGGCGGACTTGGGCGACTTCATACTCAAAACTTCCCAACATCAAACCATTACG
GTGCAACTGCCGATGGATTGATTATGGCTTATAGAGCGGGTGCTGATCTGGCATTTATGGATACCGTTCAGTATCACCCC
ACAGGTGCTTCATATCCGGAACAAATTGAAGGTTTCCTAATCACGGAGAAAGTACGAGGTTTAGGCGCAACTCCCCTAAA
TATAGACGGTGAGCAGTTCGTATATCACCTTGAAACTCGAGACGTAGAAGCTTCAGCTATAATTCGAGAGTGCGCCAAAG
GGAAAGGAATTACCACACCTTCTGGAATGCAGGGTGTTTGGCTAGACTCACCTATGATTGAACAAATTCACGGTGAAGGA
ACTATTGAAAAAGAACTACCAGCAATGGTTAGGCAGTTTGCTCGCTTTGGAGTGGATATTACAAAAGAACCTATTTTAGT
ATATCCAACCCTTCACTATCAAAATGGCGGAATTCTAATCAATGACCGCGGTGAAAGTTCCGTGGAAAACTTGTATGTGG
CCGGTGAAGCTTCCGGTGGAGTACACGGTAGAAATAGATTAATGGGTAACTCCCTATTGGATATCTTGGTCTTGGGAAGA
AGAGCTGGTAAGAACGCAGCATCCAGGGCAAAAGAAATTGACAATACAAACGCCGAATTAAACCTAGAACATGTCAAAAA
CTACGTGAACGAACTTGAAAATGAGGGAATTGAACAGCTAAGAACATCGCCCATTTTGATCCCGGATTACACTCAAAAGA
GCACTGATCAACAGTAA

Upstream 100 bases:

>100_bases
ATTCGATCAGGAGTTAAACGAGCTTGCCAATGCTAGCAAAGAAGAGCTGGCAGAACGATACAACAATCGAGAAATAGAAA
AATAAGGAGGGCAGTTTAAA

Downstream 100 bases:

>100_bases
CATCAACAGTAACATTGACATTAACATTAATATTAATAGTAACGGTACAGTAACGGAATAGCTTTTGCGTAGCAAAAGCT
AATAATAAGGAAAATCCTTT

Product: L-aspartate oxidase

Products: fumarate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MSYTPEMRELIKKVEETRDQRIGNYFPRMTPEEREEVLNNNHPDYKQDQFRDAKIGPNKGDKFPHELCDLIEAYPSIDWN
QFTTDQIDYDVDVLVIGGGGAGASASLLAHEAGADVLMATKLRFGDANTVMAQGGIQAADKSNDSPSTHYLDVIGGGHYY
NVPELVRALVSDAPDVISWLEDLGVMFDKDDEGTMQTKHGGGTSRKRMHSARDYTGAEIMRVLRDEVHCRDINVVEFSPA
VELLLDKQGRASGAILYNMETKEYQVVKAKTVIITTGGLGRLHTQNFPTSNHYGATADGLIMAYRAGADLAFMDTVQYHP
TGASYPEQIEGFLITEKVRGLGATPLNIDGEQFVYHLETRDVEASAIIRECAKGKGITTPSGMQGVWLDSPMIEQIHGEG
TIEKELPAMVRQFARFGVDITKEPILVYPTLHYQNGGILINDRGESSVENLYVAGEASGGVHGRNRLMGNSLLDILVLGR
RAGKNAASRAKEIDNTNAELNLEHVKNYVNELENEGIEQLRTSPILIPDYTQKSTDQQ

Sequences:

>Translated_538_residues
MSYTPEMRELIKKVEETRDQRIGNYFPRMTPEEREEVLNNNHPDYKQDQFRDAKIGPNKGDKFPHELCDLIEAYPSIDWN
QFTTDQIDYDVDVLVIGGGGAGASASLLAHEAGADVLMATKLRFGDANTVMAQGGIQAADKSNDSPSTHYLDVIGGGHYY
NVPELVRALVSDAPDVISWLEDLGVMFDKDDEGTMQTKHGGGTSRKRMHSARDYTGAEIMRVLRDEVHCRDINVVEFSPA
VELLLDKQGRASGAILYNMETKEYQVVKAKTVIITTGGLGRLHTQNFPTSNHYGATADGLIMAYRAGADLAFMDTVQYHP
TGASYPEQIEGFLITEKVRGLGATPLNIDGEQFVYHLETRDVEASAIIRECAKGKGITTPSGMQGVWLDSPMIEQIHGEG
TIEKELPAMVRQFARFGVDITKEPILVYPTLHYQNGGILINDRGESSVENLYVAGEASGGVHGRNRLMGNSLLDILVLGR
RAGKNAASRAKEIDNTNAELNLEHVKNYVNELENEGIEQLRTSPILIPDYTQKSTDQQ
>Mature_537_residues
SYTPEMRELIKKVEETRDQRIGNYFPRMTPEEREEVLNNNHPDYKQDQFRDAKIGPNKGDKFPHELCDLIEAYPSIDWNQ
FTTDQIDYDVDVLVIGGGGAGASASLLAHEAGADVLMATKLRFGDANTVMAQGGIQAADKSNDSPSTHYLDVIGGGHYYN
VPELVRALVSDAPDVISWLEDLGVMFDKDDEGTMQTKHGGGTSRKRMHSARDYTGAEIMRVLRDEVHCRDINVVEFSPAV
ELLLDKQGRASGAILYNMETKEYQVVKAKTVIITTGGLGRLHTQNFPTSNHYGATADGLIMAYRAGADLAFMDTVQYHPT
GASYPEQIEGFLITEKVRGLGATPLNIDGEQFVYHLETRDVEASAIIRECAKGKGITTPSGMQGVWLDSPMIEQIHGEGT
IEKELPAMVRQFARFGVDITKEPILVYPTLHYQNGGILINDRGESSVENLYVAGEASGGVHGRNRLMGNSLLDILVLGRR
AGKNAASRAKEIDNTNAELNLEHVKNYVNELENEGIEQLRTSPILIPDYTQKSTDQQ

Specific function: Catalyzes The Oxidation Of L-Aspartate To Iminoaspartate. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=398, Percent_Identity=31.9095477386935, Blast_Score=160, Evalue=4e-39,
Organism=Escherichia coli, GI1788928, Length=423, Percent_Identity=33.096926713948, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1790597, Length=392, Percent_Identity=31.8877551020408, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1786942, Length=425, Percent_Identity=33.6470588235294, Blast_Score=162, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17505833, Length=401, Percent_Identity=31.1720698254364, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17550100, Length=440, Percent_Identity=29.0909090909091, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71986328, Length=292, Percent_Identity=28.0821917808219, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322701, Length=432, Percent_Identity=33.7962962962963, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6322416, Length=432, Percent_Identity=32.4074074074074, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320788, Length=476, Percent_Identity=30.8823529411765, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6322511, Length=479, Percent_Identity=28.8100208768267, Blast_Score=134, Evalue=4e-32,
Organism=Drosophila melanogaster, GI17137288, Length=436, Percent_Identity=31.651376146789, Blast_Score=156, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24655642, Length=436, Percent_Identity=31.651376146789, Blast_Score=156, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24655647, Length=436, Percent_Identity=31.651376146789, Blast_Score=156, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24663005, Length=480, Percent_Identity=28.9583333333333, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1

Molecular weight: Translated: 59421; Mature: 59290

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYTPEMRELIKKVEETRDQRIGNYFPRMTPEEREEVLNNNHPDYKQDQFRDAKIGPNKG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCCCCCC
DKFPHELCDLIEAYPSIDWNQFTTDQIDYDVDVLVIGGGGAGASASLLAHEAGADVLMAT
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCEEEEE
KLRFGDANTVMAQGGIQAADKSNDSPSTHYLDVIGGGHYYNVPELVRALVSDAPDVISWL
EEECCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCCEECHHHHHHHHHCCCHHHHHHH
EDLGVMFDKDDEGTMQTKHGGGTSRKRMHSARDYTGAEIMRVLRDEVHCRDINVVEFSPA
HHCCCEECCCCCCCEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECHH
VELLLDKQGRASGAILYNMETKEYQVVKAKTVIITTGGLGRLHTQNFPTSNHYGATADGL
HHHHHCCCCCCCCEEEEECCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCE
IMAYRAGADLAFMDTVQYHPTGASYPEQIEGFLITEKVRGLGATPLNIDGEQFVYHLETR
EEEEECCCCEEEEECEEECCCCCCCHHHHCCEEEEHHHHCCCCCCCCCCCCCEEEEEECC
DVEASAIIRECAKGKGITTPSGMQGVWLDSPMIEQIHGEGTIEKELPAMVRQFARFGVDI
CCHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
TKEPILVYPTLHYQNGGILINDRGESSVENLYVAGEASGGVHGRNRLMGNSLLDILVLGR
CCCCEEEEEEEEECCCCEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHC
RAGKNAASRAKEIDNTNAELNLEHVKNYVNELENEGIEQLRTSPILIPDYTQKSTDQQ
CCCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCHHHHCCCCEEECCCCCCCCCCC
>Mature Secondary Structure 
SYTPEMRELIKKVEETRDQRIGNYFPRMTPEEREEVLNNNHPDYKQDQFRDAKIGPNKG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCCCCCC
DKFPHELCDLIEAYPSIDWNQFTTDQIDYDVDVLVIGGGGAGASASLLAHEAGADVLMAT
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCEEEEE
KLRFGDANTVMAQGGIQAADKSNDSPSTHYLDVIGGGHYYNVPELVRALVSDAPDVISWL
EEECCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCCEECHHHHHHHHHCCCHHHHHHH
EDLGVMFDKDDEGTMQTKHGGGTSRKRMHSARDYTGAEIMRVLRDEVHCRDINVVEFSPA
HHCCCEECCCCCCCEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECHH
VELLLDKQGRASGAILYNMETKEYQVVKAKTVIITTGGLGRLHTQNFPTSNHYGATADGL
HHHHHCCCCCCCCEEEEECCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCE
IMAYRAGADLAFMDTVQYHPTGASYPEQIEGFLITEKVRGLGATPLNIDGEQFVYHLETR
EEEEECCCCEEEEECEEECCCCCCCHHHHCCEEEEHHHHCCCCCCCCCCCCCEEEEEECC
DVEASAIIRECAKGKGITTPSGMQGVWLDSPMIEQIHGEGTIEKELPAMVRQFARFGVDI
CCHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
TKEPILVYPTLHYQNGGILINDRGESSVENLYVAGEASGGVHGRNRLMGNSLLDILVLGR
CCCCEEEEEEEEECCCCEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHC
RAGKNAASRAKEIDNTNAELNLEHVKNYVNELENEGIEQLRTSPILIPDYTQKSTDQQ
CCCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCHHHHCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: succinate; acceptor

Specific reaction: succinate + acceptor = fumarate + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]