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The map label for this gene is cfrBIM [H]

Identifier: 188586728

GI number: 188586728

Start: 2238970

End: 2240157

Strand: Reverse

Name: cfrBIM [H]

Synonym: Nther_2118

Alternate gene names: 188586728

Gene position: 2240157-2238970 (Counterclockwise)

Preceding gene: 188586729

Following gene: 188586727

Centisome position: 70.77

GC content: 31.14

Gene sequence:

>1188_bases
ATGAAAGATAAAGCTTTTCATATTAACGGTGAAACGTCTGAAAATTACAAAAAAATATTTATCCCAATAAATAAAAATGA
GTTGACCAAATCCGAAAAGCGAGGCTTACGACAAATTAATGATGCCTTTAGTAATGATGATTTGATAATTGAGACTTATG
ACCACAAATTGATTGTGTTTTTAAATAAGCTTGATGTAAATTCCTTAAAAGACTTAATATTTCAATTTGTATCAGAGCAA
GTTAGTGAAGACAATGTCCTACTAGCTTTGTTAACTAAAATAGAAAATATTAAAAGTTATACTTACCAAGGTGGAAAAAA
AGTTTTTGTAAATTTTAATGATGAGAGGAAAGTCAAAGATAGAAAAGCAAAAGAAACTCAACGTGGCAAATTCTTTTATG
CACAAGATGCCGATTTTAAAGTAACAAATAACGCAGTTCCTAAAGAATATGAAAATCAAATAATTTGTGCTGACAGTGAA
CAATTATTATCAAAAATGCCAGAAAATTGCATAGATTTAGTTTTTACATCTCCACCTTATAACTTTGGTTTAGAATATGA
TAATCATCAAGACGCCATGAATTGGTCTATTTATTTTGATAAGCTTTATAGAATTCTGGATGAATGCATTCGTGTGACTA
AGTATGGTGGTAGAATAATTGTCAATGTACAGCCCCTGTTTTCGGATTATATACCTATTCATCATTTTATTAGCAACTTT
TTTATTAAGAAGAAGTTGATATGGAAAGCCGAAATCTTATGGGAAAAAAATAATTATAATTGCAAGTATACGGCATGGGG
AAGCTGGAAAAGTCCTTCTAATCCTTATATGAAATATACCTGGGAATTTTTAGAGGTATTTTGCAAAGGAACCATGAAAA
AACAAGGCAAAAAAGAGAATATAGACATAAGCGCTGAGGAATTTAAAGAATGGGTTTATGGAAAATGGAGTATCGCCCCG
GAAAAAAATATGAAAAATTATAAACATCCATCAATGTTTCCAGAAGAGTTGGCAAGGAGAGTTATGAAATTGTTTAGTTA
TCAAGGGGATATAATTCTAGACCCCTTTGTTGGAAGTGGTACTACCCCTGTTGTTGCTCACCAAAACAATCGTCGATTTT
TGGGTATAGATATTTCTCGCGAATATTGTGAATTAGCTAGAAAAAGGATAGATCAACAAGATTGCTAG

Upstream 100 bases:

>100_bases
CTAAAGGACTTGAGTTAATTAATGGCGAAGTGACTTACTATGATGAAATAATTATGAACGGTGATAGTAAGTATGAAGCT
TAAAGGAGTTGTCCATTGGG

Downstream 100 bases:

>100_bases
AATATAAAGACATTTAGGAAAAGGATGTATTGTTAGTTATAAGAATTTTTATTATTTTCATAAATAACATTTTAAGGAAG
GAATTATAAAATATTTGTAG

Product: DNA methylase N-4/N-6 domain protein

Products: NA

Alternate protein names: M.CfrBI; N(4)- cytosine-specific methyltransferase CfrBI [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MKDKAFHINGETSENYKKIFIPINKNELTKSEKRGLRQINDAFSNDDLIIETYDHKLIVFLNKLDVNSLKDLIFQFVSEQ
VSEDNVLLALLTKIENIKSYTYQGGKKVFVNFNDERKVKDRKAKETQRGKFFYAQDADFKVTNNAVPKEYENQIICADSE
QLLSKMPENCIDLVFTSPPYNFGLEYDNHQDAMNWSIYFDKLYRILDECIRVTKYGGRIIVNVQPLFSDYIPIHHFISNF
FIKKKLIWKAEILWEKNNYNCKYTAWGSWKSPSNPYMKYTWEFLEVFCKGTMKKQGKKENIDISAEEFKEWVYGKWSIAP
EKNMKNYKHPSMFPEELARRVMKLFSYQGDIILDPFVGSGTTPVVAHQNNRRFLGIDISREYCELARKRIDQQDC

Sequences:

>Translated_395_residues
MKDKAFHINGETSENYKKIFIPINKNELTKSEKRGLRQINDAFSNDDLIIETYDHKLIVFLNKLDVNSLKDLIFQFVSEQ
VSEDNVLLALLTKIENIKSYTYQGGKKVFVNFNDERKVKDRKAKETQRGKFFYAQDADFKVTNNAVPKEYENQIICADSE
QLLSKMPENCIDLVFTSPPYNFGLEYDNHQDAMNWSIYFDKLYRILDECIRVTKYGGRIIVNVQPLFSDYIPIHHFISNF
FIKKKLIWKAEILWEKNNYNCKYTAWGSWKSPSNPYMKYTWEFLEVFCKGTMKKQGKKENIDISAEEFKEWVYGKWSIAP
EKNMKNYKHPSMFPEELARRVMKLFSYQGDIILDPFVGSGTTPVVAHQNNRRFLGIDISREYCELARKRIDQQDC
>Mature_395_residues
MKDKAFHINGETSENYKKIFIPINKNELTKSEKRGLRQINDAFSNDDLIIETYDHKLIVFLNKLDVNSLKDLIFQFVSEQ
VSEDNVLLALLTKIENIKSYTYQGGKKVFVNFNDERKVKDRKAKETQRGKFFYAQDADFKVTNNAVPKEYENQIICADSE
QLLSKMPENCIDLVFTSPPYNFGLEYDNHQDAMNWSIYFDKLYRILDECIRVTKYGGRIIVNVQPLFSDYIPIHHFISNF
FIKKKLIWKAEILWEKNNYNCKYTAWGSWKSPSNPYMKYTWEFLEVFCKGTMKKQGKKENIDISAEEFKEWVYGKWSIAP
EKNMKNYKHPSMFPEELARRVMKLFSYQGDIILDPFVGSGTTPVVAHQNNRRFLGIDISREYCELARKRIDQQDC

Specific function: This methylase recognizes the double-stranded sequence CCWWGG, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the CfrBI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=261, Percent_Identity=29.5019157088123, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 46645; Mature: 46645

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDKAFHINGETSENYKKIFIPINKNELTKSEKRGLRQINDAFSNDDLIIETYDHKLIVF
CCCCEEEECCCCCCCCEEEEEEECCHHCCHHHHHHHHHHHHHCCCCCEEEEECCCEEEEE
LNKLDVNSLKDLIFQFVSEQVSEDNVLLALLTKIENIKSYTYQGGKKVFVNFNDERKVKD
EECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEECCCCEEEEEECCCHHHHH
RKAKETQRGKFFYAQDADFKVTNNAVPKEYENQIICADSEQLLSKMPENCIDLVFTSPPY
HHHHHHHCCCEEEECCCCEEECCCCCCHHHCCCEEEECHHHHHHHCCHHHEEEEECCCCC
NFGLEYDNHQDAMNWSIYFDKLYRILDECIRVTKYGGRIIVNVQPLFSDYIPIHHFISNF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHCCHHHHHHHHH
FIKKKLIWKAEILWEKNNYNCKYTAWGSWKSPSNPYMKYTWEFLEVFCKGTMKKQGKKEN
HHHHHHEEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
IDISAEEFKEWVYGKWSIAPEKNMKNYKHPSMFPEELARRVMKLFSYQGDIILDPFVGSG
CCCCHHHHHHHHCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCC
TTPVVAHQNNRRFLGIDISREYCELARKRIDQQDC
CCCEEEECCCCEEEEEECCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKDKAFHINGETSENYKKIFIPINKNELTKSEKRGLRQINDAFSNDDLIIETYDHKLIVF
CCCCEEEECCCCCCCCEEEEEEECCHHCCHHHHHHHHHHHHHCCCCCEEEEECCCEEEEE
LNKLDVNSLKDLIFQFVSEQVSEDNVLLALLTKIENIKSYTYQGGKKVFVNFNDERKVKD
EECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEECCCCEEEEEECCCHHHHH
RKAKETQRGKFFYAQDADFKVTNNAVPKEYENQIICADSEQLLSKMPENCIDLVFTSPPY
HHHHHHHCCCEEEECCCCEEECCCCCCHHHCCCEEEECHHHHHHHCCHHHEEEEECCCCC
NFGLEYDNHQDAMNWSIYFDKLYRILDECIRVTKYGGRIIVNVQPLFSDYIPIHHFISNF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHCCHHHHHHHHH
FIKKKLIWKAEILWEKNNYNCKYTAWGSWKSPSNPYMKYTWEFLEVFCKGTMKKQGKKEN
HHHHHHEEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
IDISAEEFKEWVYGKWSIAPEKNMKNYKHPSMFPEELARRVMKLFSYQGDIILDPFVGSG
CCCCHHHHHHHHCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCC
TTPVVAHQNNRRFLGIDISREYCELARKRIDQQDC
CCCEEEECCCCEEEEEECCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8335262 [H]