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The map label for this gene is rpoN [H]

Identifier: 188586630

GI number: 188586630

Start: 2130614

End: 2132053

Strand: Reverse

Name: rpoN [H]

Synonym: Nther_2020

Alternate gene names: 188586630

Gene position: 2132053-2130614 (Counterclockwise)

Preceding gene: 188586631

Following gene: 188586629

Centisome position: 67.35

GC content: 33.89

Gene sequence:

>1440_bases
ATGAAATTAGGCTTTGATTTGAATCTGGAGCAAAAGCAACAGTTGGTTATTACCCCGGAACTTAGAAAAGCTATCAAACT
TTTGCAGTTGTCCAGTATAGAATTAGATAATTATATTGAAAAAGAATTGGTTGAAAACCCCATGTTGGAAATTTCCTCAA
CTGAAGAAAGTGAAAGTGGGGATGATAACGGAAAAGAAGACAGTGTGGAAAAATTAGAGAACAGTCAGGAGTCAGCACAA
AGTGAAGACCGCTTTGATATTGATTGGCAAAAATATTTTGAAGATTCCAGTGATGTAGGTAAATATAATAACTTGCCTAA
AGAGAATTATGACGAGGGTAAGGATGGTTTCGAAACCTTCACCCCTTCTACAATAAGCTTAACAGAACATCTTTACTTCC
AATTGTCTCTTTACTCAGATCAAGAAGAACACATTAAACCACTGGCTGAATTTTTGATAGGAAATTTGGATGCCAATGGA
TATTTAAATGGGACTTTACAAGAAATTGCAGAATTTTTAGATGTGGAAGAAAAGGAGTTAGAAAAGGCCTTAGAATTGGT
GCAAAGCCTTGATCCTCCTGGTGTAGGAGCAAGGAGCCTTAAAGAATGCTTGCTGTTACAAGTGGAAACTGATTCTCATA
GCCCCGATTCTGCTTATGAACTAATTGAAAACTTTCTATCGGAAATCGCGGAAAATCGCCTTGACAAAATTGCAAAAGAG
TTAAAATTACCTATTAAAGAGGTGCAAGAGATAGTTGATTATATCAGGTCTTTAACTCCTAAGCCTGCAAGTCCTTTCTC
TGAAGAAGGCCTTCCCTCTTATATAACACCTGATATTGTTATTAAAAGAGTTGAAGATGAATATGAAATTATTTTAAATG
ATTCCATGAGTCCTCGTCTTAAAATTAATTCAAAATATAGACAATTATTAAAAACAGAAAAGGGTTCGGGAGTAGCTAAA
TTTCTTAATTCCAGGTTAGACTCGGCTATGTGGTTAATTAAAAGTATAGAGCAAAGGCGAATCACTCTTTATAATATTAT
GCAAAAACTTGTAGAAATGCAGAGGCCTTTCTTAGATAATGGAGTGAGATATCTCAAACCATTAACTCTAAAAGAAGTAG
CTGACGAAATAGATGTGCATGAATCTACCGTAAGTAGAGCCACAGCAAATAAATATGTGCAAACTCCCCAGGGAGTATAT
CCACTAAGATTTTTCTTTTCCAGTAAACTAGATAATAATCAAGATGATTATAACTCTTCTACTAGTATTAAACAAAAGAT
CAAAGAGTTAGTTGAAGAAGAGGATAAGAAAAAACCTCTTAGTGATCAAAAAATTGCTGAAATTTTACAGGAATCATCTA
TAAATATATCAAGAAGAACTGTAGCAAAATATAGAAAAGAACTTAATTTACCTTCTTCTTCAAAGAGGAAAAGGTATTAA

Upstream 100 bases:

>100_bases
AATAAAATAGTTAATTTGGAATGAAATTTGCAAGAGGATTTTAAGCTTTAATGTAGAATATAGATAATACAGGAAACTAA
GCAAAGGGGTGTTATAGTAG

Downstream 100 bases:

>100_bases
TTGACTGAATATTTCATCTACCGGGTTAAAATAAATTAATCCTTAGTTTATTTGTCCCGGTAGATTATTTATTTTTGTTT
AAAACTAATCCTATTGAAGT

Product: RNA polymerase, sigma 54 subunit, RpoN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKLGFDLNLEQKQQLVITPELRKAIKLLQLSSIELDNYIEKELVENPMLEISSTEESESGDDNGKEDSVEKLENSQESAQ
SEDRFDIDWQKYFEDSSDVGKYNNLPKENYDEGKDGFETFTPSTISLTEHLYFQLSLYSDQEEHIKPLAEFLIGNLDANG
YLNGTLQEIAEFLDVEEKELEKALELVQSLDPPGVGARSLKECLLLQVETDSHSPDSAYELIENFLSEIAENRLDKIAKE
LKLPIKEVQEIVDYIRSLTPKPASPFSEEGLPSYITPDIVIKRVEDEYEIILNDSMSPRLKINSKYRQLLKTEKGSGVAK
FLNSRLDSAMWLIKSIEQRRITLYNIMQKLVEMQRPFLDNGVRYLKPLTLKEVADEIDVHESTVSRATANKYVQTPQGVY
PLRFFFSSKLDNNQDDYNSSTSIKQKIKELVEEEDKKKPLSDQKIAEILQESSINISRRTVAKYRKELNLPSSSKRKRY

Sequences:

>Translated_479_residues
MKLGFDLNLEQKQQLVITPELRKAIKLLQLSSIELDNYIEKELVENPMLEISSTEESESGDDNGKEDSVEKLENSQESAQ
SEDRFDIDWQKYFEDSSDVGKYNNLPKENYDEGKDGFETFTPSTISLTEHLYFQLSLYSDQEEHIKPLAEFLIGNLDANG
YLNGTLQEIAEFLDVEEKELEKALELVQSLDPPGVGARSLKECLLLQVETDSHSPDSAYELIENFLSEIAENRLDKIAKE
LKLPIKEVQEIVDYIRSLTPKPASPFSEEGLPSYITPDIVIKRVEDEYEIILNDSMSPRLKINSKYRQLLKTEKGSGVAK
FLNSRLDSAMWLIKSIEQRRITLYNIMQKLVEMQRPFLDNGVRYLKPLTLKEVADEIDVHESTVSRATANKYVQTPQGVY
PLRFFFSSKLDNNQDDYNSSTSIKQKIKELVEEEDKKKPLSDQKIAEILQESSINISRRTVAKYRKELNLPSSSKRKRY
>Mature_479_residues
MKLGFDLNLEQKQQLVITPELRKAIKLLQLSSIELDNYIEKELVENPMLEISSTEESESGDDNGKEDSVEKLENSQESAQ
SEDRFDIDWQKYFEDSSDVGKYNNLPKENYDEGKDGFETFTPSTISLTEHLYFQLSLYSDQEEHIKPLAEFLIGNLDANG
YLNGTLQEIAEFLDVEEKELEKALELVQSLDPPGVGARSLKECLLLQVETDSHSPDSAYELIENFLSEIAENRLDKIAKE
LKLPIKEVQEIVDYIRSLTPKPASPFSEEGLPSYITPDIVIKRVEDEYEIILNDSMSPRLKINSKYRQLLKTEKGSGVAK
FLNSRLDSAMWLIKSIEQRRITLYNIMQKLVEMQRPFLDNGVRYLKPLTLKEVADEIDVHESTVSRATANKYVQTPQGVY
PLRFFFSSKLDNNQDDYNSSTSIKQKIKELVEEEDKKKPLSDQKIAEILQESSINISRRTVAKYRKELNLPSSSKRKRY

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=490, Percent_Identity=33.8775510204082, Blast_Score=275, Evalue=7e-75,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 55124; Mature: 55124

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLGFDLNLEQKQQLVITPELRKAIKLLQLSSIELDNYIEKELVENPMLEISSTEESESG
CCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCC
DDNGKEDSVEKLENSQESAQSEDRFDIDWQKYFEDSSDVGKYNNLPKENYDEGKDGFETF
CCCCCHHHHHHHHCHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHC
TPSTISLTEHLYFQLSLYSDQEEHIKPLAEFLIGNLDANGYLNGTLQEIAEFLDVEEKEL
CCCHHHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHH
EKALELVQSLDPPGVGARSLKECLLLQVETDSHSPDSAYELIENFLSEIAENRLDKIAKE
HHHHHHHHCCCCCCCCHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LKLPIKEVQEIVDYIRSLTPKPASPFSEEGLPSYITPDIVIKRVEDEYEIILNDSMSPRL
HCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCE
KINSKYRQLLKTEKGSGVAKFLNSRLDSAMWLIKSIEQRRITLYNIMQKLVEMQRPFLDN
EECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHC
GVRYLKPLTLKEVADEIDVHESTVSRATANKYVQTPQGVYPLRFFFSSKLDNNQDDYNSS
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCH
TSIKQKIKELVEEEDKKKPLSDQKIAEILQESSINISRRTVAKYRKELNLPSSSKRKRY
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKLGFDLNLEQKQQLVITPELRKAIKLLQLSSIELDNYIEKELVENPMLEISSTEESESG
CCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCC
DDNGKEDSVEKLENSQESAQSEDRFDIDWQKYFEDSSDVGKYNNLPKENYDEGKDGFETF
CCCCCHHHHHHHHCHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHC
TPSTISLTEHLYFQLSLYSDQEEHIKPLAEFLIGNLDANGYLNGTLQEIAEFLDVEEKEL
CCCHHHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHH
EKALELVQSLDPPGVGARSLKECLLLQVETDSHSPDSAYELIENFLSEIAENRLDKIAKE
HHHHHHHHCCCCCCCCHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LKLPIKEVQEIVDYIRSLTPKPASPFSEEGLPSYITPDIVIKRVEDEYEIILNDSMSPRL
HCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCE
KINSKYRQLLKTEKGSGVAKFLNSRLDSAMWLIKSIEQRRITLYNIMQKLVEMQRPFLDN
EECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHC
GVRYLKPLTLKEVADEIDVHESTVSRATANKYVQTPQGVYPLRFFFSSKLDNNQDDYNSS
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCH
TSIKQKIKELVEEEDKKKPLSDQKIAEILQESSINISRRTVAKYRKELNLPSSSKRKRY
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550525 [H]