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The map label for this gene is pgk [H]

Identifier: 188586628

GI number: 188586628

Start: 2128116

End: 2129300

Strand: Reverse

Name: pgk [H]

Synonym: Nther_2018

Alternate gene names: 188586628

Gene position: 2129300-2128116 (Counterclockwise)

Preceding gene: 188586629

Following gene: 188586627

Centisome position: 67.26

GC content: 38.06

Gene sequence:

>1185_bases
ATGAAAAAAACTGTTAAAGACGTGGATGTTACAGGTAAACGAGTATTTGTTAGGGTTGATTTTAATGTTCCTTTAGATAA
CGGGAAGATAACTGATGAAACAAGGGTACAAAAGTCACTTCCAACAATTAATTACTTGAGAGAACAAGGGGCACGGGTCA
TACTAGCTTCACATTTAGGCCGACCTAAGGGACAAGTAAAAGATGAGTTTCGGATGGTCCCAGTTAAAGAAAAGTTAGAA
GAACTATTAAAAACATCAGTCAAAAAGAGTTCTGAAAACGATCCTAGTCAAGCTGAAGCCGCTGTTAATTCCCTTGAAGA
TGGTGAGGTACTTTTATTAGAGAACCTGCGTTTTCATCCAGGGGAAGAAGAAAATGATGAGGAATTTGCGAATGCTTTAG
CTTCATTGGTTGATATTTATGTGAATGATGCCTTTGGTGCGGCTCATAGAGCTCATGCCTCTACTCATGGTATTACCCAA
AAAATCTCTGGCGAATGTGTGGCCGGTTTATTAATGGCTCAAGAACTTAAATTTTTAGGGGAAAGTGTTGATAATCCTGA
AAGACCCTTTGTAGCTGTAATTGGCGGAGCTAAGATTAGTGATAAAATCGGTGTGATTGACAGGCTCATAGAAACTGCTA
ATAAAGTAGTGATAGGAGGGGGCATGGCCAATACATTTTTACTAGCCAAAGGTTATCAATTGGGAAAATCATTGGTTGAA
GAGGATGCCATTGATAAAGCTAAAGAACTATTGAAAAAAGCAGACAACTTAGGTGTAGAATTAATGTTACCAAAAGATGT
AGTAGTTGCCGAGAAACTTGAGGAAAAAGTTGCAAATAAAACTGTTAAAGTAGAAGAGATTCCTGAAAATATGATGGCTC
TAGACATCGGAGAAGAAACTATCAATGAATATTCTAAGGTTATAAAGAATGGAAAGACTGTCATCATGAATGGTCCCATG
GGAGTTTTTGAAACAGAACCCTTTGATCATGGAACAGTTGAAATTGCAAAAGCCATGGGTAATAGTTCGGGAACAACTAT
AGTTGGAGGCGGAGATTCAGTTTCAGCTATTAATAAAGCAGGCCTAGAAGATAAAATTACCCATATATCTACAGGTGGCG
GAGCTTCTTTAAAATATTTGGAAGGCAAGCCATTACCTGGAGTTCAGGTTCTCCAAGATAGGTAA

Upstream 100 bases:

>100_bases
AGTTACAGACGAGGGTGCGGCTGAAAAAATTCAAGCTATAATGAATACTTAGACCTGAGTTGTTAAAGTATTTAGATAAT
CAGAGTAAGGAGGAATTATA

Downstream 100 bases:

>100_bases
AGGTAAATATTGAATTATGATTGCAATATATAATTCTAGTGGAGGTGAGATTAAGTGGCCAGGACTATTATTGCAGGGAA
CTGGAAGATGAACCATGGTC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKKTVKDVDVTGKRVFVRVDFNVPLDNGKITDETRVQKSLPTINYLREQGARVILASHLGRPKGQVKDEFRMVPVKEKLE
ELLKTSVKKSSENDPSQAEAAVNSLEDGEVLLLENLRFHPGEEENDEEFANALASLVDIYVNDAFGAAHRAHASTHGITQ
KISGECVAGLLMAQELKFLGESVDNPERPFVAVIGGAKISDKIGVIDRLIETANKVVIGGGMANTFLLAKGYQLGKSLVE
EDAIDKAKELLKKADNLGVELMLPKDVVVAEKLEEKVANKTVKVEEIPENMMALDIGEETINEYSKVIKNGKTVIMNGPM
GVFETEPFDHGTVEIAKAMGNSSGTTIVGGGDSVSAINKAGLEDKITHISTGGGASLKYLEGKPLPGVQVLQDR

Sequences:

>Translated_394_residues
MKKTVKDVDVTGKRVFVRVDFNVPLDNGKITDETRVQKSLPTINYLREQGARVILASHLGRPKGQVKDEFRMVPVKEKLE
ELLKTSVKKSSENDPSQAEAAVNSLEDGEVLLLENLRFHPGEEENDEEFANALASLVDIYVNDAFGAAHRAHASTHGITQ
KISGECVAGLLMAQELKFLGESVDNPERPFVAVIGGAKISDKIGVIDRLIETANKVVIGGGMANTFLLAKGYQLGKSLVE
EDAIDKAKELLKKADNLGVELMLPKDVVVAEKLEEKVANKTVKVEEIPENMMALDIGEETINEYSKVIKNGKTVIMNGPM
GVFETEPFDHGTVEIAKAMGNSSGTTIVGGGDSVSAINKAGLEDKITHISTGGGASLKYLEGKPLPGVQVLQDR
>Mature_394_residues
MKKTVKDVDVTGKRVFVRVDFNVPLDNGKITDETRVQKSLPTINYLREQGARVILASHLGRPKGQVKDEFRMVPVKEKLE
ELLKTSVKKSSENDPSQAEAAVNSLEDGEVLLLENLRFHPGEEENDEEFANALASLVDIYVNDAFGAAHRAHASTHGITQ
KISGECVAGLLMAQELKFLGESVDNPERPFVAVIGGAKISDKIGVIDRLIETANKVVIGGGMANTFLLAKGYQLGKSLVE
EDAIDKAKELLKKADNLGVELMLPKDVVVAEKLEEKVANKTVKVEEIPENMMALDIGEETINEYSKVIKNGKTVIMNGPM
GVFETEPFDHGTVEIAKAMGNSSGTTIVGGGDSVSAINKAGLEDKITHISTGGGASLKYLEGKPLPGVQVLQDR

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=417, Percent_Identity=41.2470023980815, Blast_Score=305, Evalue=3e-83,
Organism=Homo sapiens, GI31543397, Length=415, Percent_Identity=40.2409638554217, Blast_Score=297, Evalue=1e-80,
Organism=Escherichia coli, GI1789294, Length=391, Percent_Identity=43.4782608695652, Blast_Score=329, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17508823, Length=413, Percent_Identity=43.5835351089588, Blast_Score=329, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6319857, Length=413, Percent_Identity=42.3728813559322, Blast_Score=320, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=42.1686746987952, Blast_Score=302, Evalue=3e-82,
Organism=Drosophila melanogaster, GI17136394, Length=413, Percent_Identity=40.9200968523002, Blast_Score=285, Evalue=4e-77,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 42541; Mature: 42541

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTVKDVDVTGKRVFVRVDFNVPLDNGKITDETRVQKSLPTINYLREQGARVILASHLG
CCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHCCHHHHHHHCCCEEEEEHHCC
RPKGQVKDEFRMVPVKEKLEELLKTSVKKSSENDPSQAEAAVNSLEDGEVLLLENLRFHP
CCCCCCCHHHEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCCC
GEEENDEEFANALASLVDIYVNDAFGAAHRAHASTHGITQKISGECVAGLLMAQELKFLG
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHCHHHHHHHHHHHHHHHHH
ESVDNPERPFVAVIGGAKISDKIGVIDRLIETANKVVIGGGMANTFLLAKGYQLGKSLVE
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHCCHHHHHHHHH
EDAIDKAKELLKKADNLGVELMLPKDVVVAEKLEEKVANKTVKVEEIPENMMALDIGEET
HHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCEEHHHCCCCCEEEECCHHH
INEYSKVIKNGKTVIMNGPMGVFETEPFDHGTVEIAKAMGNSSGTTIVGGGDSVSAINKA
HHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCHHHHHHC
GLEDKITHISTGGGASLKYLEGKPLPGVQVLQDR
CCCHHHEEEECCCCCEEEEECCCCCCCCHHCCCC
>Mature Secondary Structure
MKKTVKDVDVTGKRVFVRVDFNVPLDNGKITDETRVQKSLPTINYLREQGARVILASHLG
CCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHCCHHHHHHHCCCEEEEEHHCC
RPKGQVKDEFRMVPVKEKLEELLKTSVKKSSENDPSQAEAAVNSLEDGEVLLLENLRFHP
CCCCCCCHHHEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCCC
GEEENDEEFANALASLVDIYVNDAFGAAHRAHASTHGITQKISGECVAGLLMAQELKFLG
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHCHHHHHHHHHHHHHHHHH
ESVDNPERPFVAVIGGAKISDKIGVIDRLIETANKVVIGGGMANTFLLAKGYQLGKSLVE
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHCCHHHHHHHHH
EDAIDKAKELLKKADNLGVELMLPKDVVVAEKLEEKVANKTVKVEEIPENMMALDIGEET
HHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCEEHHHCCCCCEEEECCHHH
INEYSKVIKNGKTVIMNGPMGVFETEPFDHGTVEIAKAMGNSSGTTIVGGGDSVSAINKA
HHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCHHHHHHC
GLEDKITHISTGGGASLKYLEGKPLPGVQVLQDR
CCCHHHEEEECCCCCEEEEECCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA