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The map label for this gene is tpiA

Identifier: 188586627

GI number: 188586627

Start: 2127291

End: 2128061

Strand: Reverse

Name: tpiA

Synonym: Nther_2017

Alternate gene names: 188586627

Gene position: 2128061-2127291 (Counterclockwise)

Preceding gene: 188586628

Following gene: 188586626

Centisome position: 67.23

GC content: 40.73

Gene sequence:

>771_bases
GTGGCCAGGACTATTATTGCAGGGAACTGGAAGATGAACCATGGTCCTAGAGAAACCAGGGAGTTTGTGGCTGGTATGAA
AGAAAAATACCAAACCCCACCGGCAGTAGAAACCGTGATTTGCCCTCCTTTTGTATCAATATCTGATCTGGTAAATGAGA
GCCCTGAATGGCTTAAAACCGGAGCTCAAAATGTTTATTTCGAAGAGAGTGGTGCTTTTACAGGAGAAGTTTCTCCAAAA
ATGTTGGCAGAATTGGGAGTAGAATATGTAATCATTGGGCATTCAGAGAGAAGAAATATCTTTGCAGAGTCTGATGAGGA
AGTGAATAAAAAAGTTAGGATTGCGCTAAAATATGGTATTAAACCAATTATTTGTGTTGGAGAAAGCGATGCTCAGAGAA
ATGAAGGTAAAACCTTAGAAGTAGTGGAAAATCAAGTTAAATCAGCTCTGTCAGAAGTAGTTTCTGATACCTTGCAAAAT
GTAGTATTTGCCTATGAACCAGTATGGGCAATAGGATCAGGTAAAGCAGCTACAGGCGAGGATGCCGAGCAAGTTTGTAA
GCATATTAGATCAGTGATAAGTGAACTTAACTCAAGCGTGGCAGATGATATACCTGTGTTATACGGGGGTAGTGTAAAAC
CAGAGAATTTAGAAGAATTTATGGACCAGGACAATATTAATGGGGCCCTTGTAGGAGGGAAAAGCTTAGTAGCAGAAACC
TACTGTCAATTACTGGAGGTAGCTAGGAGGTTCCCAGGTGATGAAGGATAA

Upstream 100 bases:

>100_bases
GGAAGGCAAGCCATTACCTGGAGTTCAGGTTCTCCAAGATAGGTAAAGGTAAATATTGAATTATGATTGCAATATATAAT
TCTAGTGGAGGTGAGATTAA

Downstream 100 bases:

>100_bases
GAGTGTAAAACGTCCATTAGTGTTAATAATAATGGATGGACTAGGTATTAGTGGAGAACATGAGGGAAACGCTTATTATC
AAGCTGACACACCTTTTTTA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MARTIIAGNWKMNHGPRETREFVAGMKEKYQTPPAVETVICPPFVSISDLVNESPEWLKTGAQNVYFEESGAFTGEVSPK
MLAELGVEYVIIGHSERRNIFAESDEEVNKKVRIALKYGIKPIICVGESDAQRNEGKTLEVVENQVKSALSEVVSDTLQN
VVFAYEPVWAIGSGKAATGEDAEQVCKHIRSVISELNSSVADDIPVLYGGSVKPENLEEFMDQDNINGALVGGKSLVAET
YCQLLEVARRFPGDEG

Sequences:

>Translated_256_residues
MARTIIAGNWKMNHGPRETREFVAGMKEKYQTPPAVETVICPPFVSISDLVNESPEWLKTGAQNVYFEESGAFTGEVSPK
MLAELGVEYVIIGHSERRNIFAESDEEVNKKVRIALKYGIKPIICVGESDAQRNEGKTLEVVENQVKSALSEVVSDTLQN
VVFAYEPVWAIGSGKAATGEDAEQVCKHIRSVISELNSSVADDIPVLYGGSVKPENLEEFMDQDNINGALVGGKSLVAET
YCQLLEVARRFPGDEG
>Mature_255_residues
ARTIIAGNWKMNHGPRETREFVAGMKEKYQTPPAVETVICPPFVSISDLVNESPEWLKTGAQNVYFEESGAFTGEVSPKM
LAELGVEYVIIGHSERRNIFAESDEEVNKKVRIALKYGIKPIICVGESDAQRNEGKTLEVVENQVKSALSEVVSDTLQNV
VFAYEPVWAIGSGKAATGEDAEQVCKHIRSVISELNSSVADDIPVLYGGSVKPENLEEFMDQDNINGALVGGKSLVAETY
CQLLEVARRFPGDEG

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=241, Percent_Identity=39.0041493775934, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI4507645, Length=241, Percent_Identity=39.0041493775934, Blast_Score=162, Evalue=2e-40,
Organism=Escherichia coli, GI1790353, Length=250, Percent_Identity=39.2, Blast_Score=190, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI17536593, Length=235, Percent_Identity=40, Blast_Score=170, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6320255, Length=243, Percent_Identity=43.2098765432099, Blast_Score=182, Evalue=4e-47,
Organism=Drosophila melanogaster, GI28572008, Length=241, Percent_Identity=41.49377593361, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI28572006, Length=241, Percent_Identity=41.49377593361, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI28572004, Length=241, Percent_Identity=41.49377593361, Blast_Score=166, Evalue=2e-41,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_NATTJ (B2A6Z3)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001918172.1
- ProteinModelPortal:   B2A6Z3
- SMR:   B2A6Z3
- GeneID:   6315872
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_2017
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 28041; Mature: 27910

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 94-94 ACT_SITE 166-166 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTIIAGNWKMNHGPRETREFVAGMKEKYQTPPAVETVICPPFVSISDLVNESPEWLKT
CCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHCCCHHHHHC
GAQNVYFEESGAFTGEVSPKMLAELGVEYVIIGHSERRNIFAESDEEVNKKVRIALKYGI
CCCCEEEECCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHEEEHEECCC
KPIICVGESDAQRNEGKTLEVVENQVKSALSEVVSDTLQNVVFAYEPVWAIGSGKAATGE
CEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCC
DAEQVCKHIRSVISELNSSVADDIPVLYGGSVKPENLEEFMDQDNINGALVGGKSLVAET
CHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHCCCCCCEEEECCHHHHHHH
YCQLLEVARRFPGDEG
HHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ARTIIAGNWKMNHGPRETREFVAGMKEKYQTPPAVETVICPPFVSISDLVNESPEWLKT
CCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHCCCHHHHHC
GAQNVYFEESGAFTGEVSPKMLAELGVEYVIIGHSERRNIFAESDEEVNKKVRIALKYGI
CCCCEEEECCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHEEEHEECCC
KPIICVGESDAQRNEGKTLEVVENQVKSALSEVVSDTLQNVVFAYEPVWAIGSGKAATGE
CEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCC
DAEQVCKHIRSVISELNSSVADDIPVLYGGSVKPENLEEFMDQDNINGALVGGKSLVAET
CHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHCCCCCCEEEECCHHHHHHH
YCQLLEVARRFPGDEG
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA