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The map label for this gene is pfkA

Identifier: 188586614

GI number: 188586614

Start: 2112388

End: 2113347

Strand: Reverse

Name: pfkA

Synonym: Nther_2004

Alternate gene names: 188586614

Gene position: 2113347-2112388 (Counterclockwise)

Preceding gene: 188586615

Following gene: 188586613

Centisome position: 66.76

GC content: 39.9

Gene sequence:

>960_bases
GTGAAAAAAATTGGAGTTTTAACCAGTGGAGGAGATTCTCCTGGCATGAATGCCACTATTAGAGCAGTGGTGCGCCGGGG
CATATATATGGGTTTAGATATTTATGGGATATATTATGGCTTTAGTGGATTGATAAATGGAAATATCGAAAAATTAGAAA
TAGGTAGTGTGGGAGATATCATCCACCGCGGTGGTACCATTTTGCACACCAGTAGGTCTGAAGAGTTTAAAACCGAAATG
GGACAACAAAAAGGTTTTGATAATTTAAAAGAACTGGGAATTGAAGGGTTAATTGTTATCGGAGGGGATGGAAGTTTCAA
GGGAGCAGAGAAATTATCTCAAATGGGGTTACCAGTAATAGGTGTTCCAGGAACTATCGATAATGATATTCCTGAAACAG
AGATGAGTATTGGCTTTGATACAGCCCTCAACACTGTCATTGAAGCTATCGACCGCATAAGAGATACTGCCACTTCCCAT
GAACGAATATTTGTAATAGAAGTTATGGGAAGAGATTGTGGTAATTTAGCTTTATGGTCAGGTTTAGCCGGTGGGGCCGA
ATCTGTACTGATTCCAGAAATAGATTACGATATGGAAAAGGTTATCGAAAGACTAAAACACGGATATAAACGTGGAAAAA
AGCATAGCATAATAATTGTGGCAGAAGGTGTCAAACCGGGAGCTGAAATTGGAAAAGAGATTAAAGAACGGGCAGGACTC
GATAATAGGGTGACTGTTCTAGGTCACGTTCAACGAGGAGGAACGCCAACTGCTTATGATAGGATGTTGGGTAGTCGTTT
AGGGGCAAAGGCAGTGGATCTCTTAGTTGCAGGGGAATCTGATAAAATGATAGGAATGAGAGATTACAAGCTTGTTCCCG
TACAATTCAAGGATGTATTTAGTAAAATTGAAGAACCTGATTACGATATTTATGAGCTTTCTCAAAGCCTGTCAATCTAA

Upstream 100 bases:

>100_bases
TATTATTTAATATATGAAAAGATAAACTAAAATTCATAGTATAAAATGGCCTGGAAGTAAAATCAAAATTAATTAGAATG
AGCTTAAGGGGGTACTTTCA

Downstream 100 bases:

>100_bases
ATATAATTGATCATTCAAATTGTACAAAACCTATATTAGAGGTGATCTCTATGAAAGAGTATCAATTAGCAGTTGAAAGA
ATTGAGGTGAATTGGAACTA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKKIGVLTSGGDSPGMNATIRAVVRRGIYMGLDIYGIYYGFSGLINGNIEKLEIGSVGDIIHRGGTILHTSRSEEFKTEM
GQQKGFDNLKELGIEGLIVIGGDGSFKGAEKLSQMGLPVIGVPGTIDNDIPETEMSIGFDTALNTVIEAIDRIRDTATSH
ERIFVIEVMGRDCGNLALWSGLAGGAESVLIPEIDYDMEKVIERLKHGYKRGKKHSIIIVAEGVKPGAEIGKEIKERAGL
DNRVTVLGHVQRGGTPTAYDRMLGSRLGAKAVDLLVAGESDKMIGMRDYKLVPVQFKDVFSKIEEPDYDIYELSQSLSI

Sequences:

>Translated_319_residues
MKKIGVLTSGGDSPGMNATIRAVVRRGIYMGLDIYGIYYGFSGLINGNIEKLEIGSVGDIIHRGGTILHTSRSEEFKTEM
GQQKGFDNLKELGIEGLIVIGGDGSFKGAEKLSQMGLPVIGVPGTIDNDIPETEMSIGFDTALNTVIEAIDRIRDTATSH
ERIFVIEVMGRDCGNLALWSGLAGGAESVLIPEIDYDMEKVIERLKHGYKRGKKHSIIIVAEGVKPGAEIGKEIKERAGL
DNRVTVLGHVQRGGTPTAYDRMLGSRLGAKAVDLLVAGESDKMIGMRDYKLVPVQFKDVFSKIEEPDYDIYELSQSLSI
>Mature_319_residues
MKKIGVLTSGGDSPGMNATIRAVVRRGIYMGLDIYGIYYGFSGLINGNIEKLEIGSVGDIIHRGGTILHTSRSEEFKTEM
GQQKGFDNLKELGIEGLIVIGGDGSFKGAEKLSQMGLPVIGVPGTIDNDIPETEMSIGFDTALNTVIEAIDRIRDTATSH
ERIFVIEVMGRDCGNLALWSGLAGGAESVLIPEIDYDMEKVIERLKHGYKRGKKHSIIIVAEGVKPGAEIGKEIKERAGL
DNRVTVLGHVQRGGTPTAYDRMLGSRLGAKAVDLLVAGESDKMIGMRDYKLVPVQFKDVFSKIEEPDYDIYELSQSLSI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI11321601, Length=315, Percent_Identity=45.7142857142857, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI48762920, Length=315, Percent_Identity=45.0793650793651, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI266453768, Length=353, Percent_Identity=41.0764872521246, Blast_Score=231, Evalue=5e-61,
Organism=Homo sapiens, GI266453748, Length=353, Percent_Identity=41.0764872521246, Blast_Score=231, Evalue=5e-61,
Organism=Homo sapiens, GI4505749, Length=353, Percent_Identity=41.0764872521246, Blast_Score=231, Evalue=5e-61,
Organism=Homo sapiens, GI266453619, Length=315, Percent_Identity=43.8095238095238, Blast_Score=231, Evalue=6e-61,
Organism=Escherichia coli, GI1790350, Length=306, Percent_Identity=49.6732026143791, Blast_Score=309, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17558788, Length=313, Percent_Identity=42.8115015974441, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI25147584, Length=310, Percent_Identity=41.6129032258065, Blast_Score=222, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI72003601, Length=289, Percent_Identity=31.8339100346021, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321679, Length=353, Percent_Identity=37.9603399433428, Blast_Score=216, Evalue=5e-57,
Organism=Saccharomyces cerevisiae, GI6323861, Length=333, Percent_Identity=37.8378378378378, Blast_Score=203, Evalue=4e-53,
Organism=Drosophila melanogaster, GI24652337, Length=318, Percent_Identity=42.1383647798742, Blast_Score=235, Evalue=2e-62,
Organism=Drosophila melanogaster, GI17647809, Length=318, Percent_Identity=42.1383647798742, Blast_Score=235, Evalue=2e-62,
Organism=Drosophila melanogaster, GI28573326, Length=318, Percent_Identity=40.8805031446541, Blast_Score=230, Evalue=8e-61,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_NATTJ (B2A6Y0)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001918159.1
- ProteinModelPortal:   B2A6Y0
- SMR:   B2A6Y0
- GeneID:   6314470
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_2004
- HOGENOM:   HBG405044
- OMA:   VDAIENM
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02482

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 34618; Mature: 34618

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 127-127 BINDING 162-162 BINDING 243-243 BINDING 249-249 BINDING 252-252

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIGVLTSGGDSPGMNATIRAVVRRGIYMGLDIYGIYYGFSGLINGNIEKLEIGSVGDI
CCCEEEEECCCCCCCCCHHHHHHHHCCHHCCHHHHHHHHHHHHHCCCCCEEEECCCHHHH
IHRGGTILHTSRSEEFKTEMGQQKGFDNLKELGIEGLIVIGGDGSFKGAEKLSQMGLPVI
HHCCCEEEECCCCHHHHHHHHHHCCCHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCEE
GVPGTIDNDIPETEMSIGFDTALNTVIEAIDRIRDTATSHERIFVIEVMGRDCGNLALWS
ECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHH
GLAGGAESVLIPEIDYDMEKVIERLKHGYKRGKKHSIIIVAEGVKPGAEIGKEIKERAGL
CCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCC
DNRVTVLGHVQRGGTPTAYDRMLGSRLGAKAVDLLVAGESDKMIGMRDYKLVPVQFKDVF
CCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEECCCCCEEEEEHHHHHH
SKIEEPDYDIYELSQSLSI
HHHCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MKKIGVLTSGGDSPGMNATIRAVVRRGIYMGLDIYGIYYGFSGLINGNIEKLEIGSVGDI
CCCEEEEECCCCCCCCCHHHHHHHHCCHHCCHHHHHHHHHHHHHCCCCCEEEECCCHHHH
IHRGGTILHTSRSEEFKTEMGQQKGFDNLKELGIEGLIVIGGDGSFKGAEKLSQMGLPVI
HHCCCEEEECCCCHHHHHHHHHHCCCHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCEE
GVPGTIDNDIPETEMSIGFDTALNTVIEAIDRIRDTATSHERIFVIEVMGRDCGNLALWS
ECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHH
GLAGGAESVLIPEIDYDMEKVIERLKHGYKRGKKHSIIIVAEGVKPGAEIGKEIKERAGL
CCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCC
DNRVTVLGHVQRGGTPTAYDRMLGSRLGAKAVDLLVAGESDKMIGMRDYKLVPVQFKDVF
CCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEECCCCCEEEEEHHHHHH
SKIEEPDYDIYELSQSLSI
HHHCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA