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The map label for this gene is hisZ [H]

Identifier: 188586612

GI number: 188586612

Start: 2109517

End: 2110746

Strand: Reverse

Name: hisZ [H]

Synonym: Nther_2002

Alternate gene names: 188586612

Gene position: 2110746-2109517 (Counterclockwise)

Preceding gene: 188586613

Following gene: 188586611

Centisome position: 66.68

GC content: 34.63

Gene sequence:

>1230_bases
TTGGTCCCAAGAGAGATTCAGCTGTTACAAAAACCATCAGGAGTCAACGATAACTTACCTAATAGAGTAGAAAAGTTTCG
ATTATTAGAAAATAAAATTTTAGAAACCTTTAAACTATGGGGGTATGATGAGGTTTCTGTGCCTTATATCGAATATTCCA
ATATATTCTCAGTAAATAAAGAAAACTTGCTGGAACAACAAATGTTCCAGTTCAATGATGAAACTGGACGTTTGGTTGTA
CTTAGACCTGATTTTACACCTTCAATTGCTAGATTAGTTGCAACTTACTATAAAAATCATCCCCTGCCATTGAGATTATG
TTATTCTGGTAAAATCTTTCGAGCAAATAATGGCAATTCAATTAATGAAAAAGAACAAACTCAAGTGGGAGTTGAATTGA
TAGGTGGTGAAGCACCGGGTGGAGATGCCGAATTAGTAGCTATGGCAGCAGAGACATTTCAAAAAATGGAGATAAAGAAT
TTTGTTATCTGTATTGGCAATCTTAAGTTTATCAATAATTTATTAGATTGTCTTAAAGTTGATCAATCCTCTCAAAGTAT
GTTAATTGAAGCTTTAAATGAAAATAATTTAGTCCAATATCAGGGGATAATTGATAGTCTGGAATTGGAATCAGACAGTA
AGGATATTCTTAAAAAGTTACCAAAATTCAGGGGTAATAAAGAAAATCTTAACTATGTTAGGGAAGTAGCTGGGTTTAAG
CCAGTTATGAATATTTTAGATGAACTCGAAAATATCTTTAAAACTCTTGAAAGTTACGGCTTGCAAGATAAGGTGACATT
TGATCTGTCTCTAGTACGAAAATTAGATTATTATACCGGTTTTATTATGGAAGGTTATGCCGAAAAAGTTGGCTTTCCAC
TTTGTGGAGGTGGCCGATATGATAAGCTGATGGATAAATACGGTATGAGCTTACCGGCTACGGGTTTCGCCTTTTCTTTT
GATAGTTTAATAGATTTAGTGAAAGTTAAAGGTGATGCGTTGTCAAAACTTCATTTTGGATATACCCAGGGTAAGCGTGA
CCAAGCCTTAGAACATGTAAAAGATTATCGTCAGCGAGGATATAGAGTGAGTTTGGAGTTAAATAGCCAGGAATTGGAAG
TATCTCGACGCAAAGCGAAAGAACAGGGAGCTGACCAATTTTATTATATAGGTAATCATGGAATGGTTAAGGAAAATATC
AGCACTAATTCGGAAAGAAAGGATGTCTAG

Upstream 100 bases:

>100_bases
AGAGACCCATTTTATTGACGGAATAAAATAGGCGTGATAAGATATTACCAATGATTAATTAACACTTTAACGAGTTAACG
AAATAAAGGAGGTTCCTTAA

Downstream 100 bases:

>100_bases
GATGAGAACGGGAGAACAACTGACAATTGCTTTACCAAAGGGTAGGATTCTTGATGAAAGTGTTAAACTACTGGAACAAG
CAGGTATAGCCTGTGATCAG

Product: Histidine--tRNA ligase

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MVPREIQLLQKPSGVNDNLPNRVEKFRLLENKILETFKLWGYDEVSVPYIEYSNIFSVNKENLLEQQMFQFNDETGRLVV
LRPDFTPSIARLVATYYKNHPLPLRLCYSGKIFRANNGNSINEKEQTQVGVELIGGEAPGGDAELVAMAAETFQKMEIKN
FVICIGNLKFINNLLDCLKVDQSSQSMLIEALNENNLVQYQGIIDSLELESDSKDILKKLPKFRGNKENLNYVREVAGFK
PVMNILDELENIFKTLESYGLQDKVTFDLSLVRKLDYYTGFIMEGYAEKVGFPLCGGGRYDKLMDKYGMSLPATGFAFSF
DSLIDLVKVKGDALSKLHFGYTQGKRDQALEHVKDYRQRGYRVSLELNSQELEVSRRKAKEQGADQFYYIGNHGMVKENI
STNSERKDV

Sequences:

>Translated_409_residues
MVPREIQLLQKPSGVNDNLPNRVEKFRLLENKILETFKLWGYDEVSVPYIEYSNIFSVNKENLLEQQMFQFNDETGRLVV
LRPDFTPSIARLVATYYKNHPLPLRLCYSGKIFRANNGNSINEKEQTQVGVELIGGEAPGGDAELVAMAAETFQKMEIKN
FVICIGNLKFINNLLDCLKVDQSSQSMLIEALNENNLVQYQGIIDSLELESDSKDILKKLPKFRGNKENLNYVREVAGFK
PVMNILDELENIFKTLESYGLQDKVTFDLSLVRKLDYYTGFIMEGYAEKVGFPLCGGGRYDKLMDKYGMSLPATGFAFSF
DSLIDLVKVKGDALSKLHFGYTQGKRDQALEHVKDYRQRGYRVSLELNSQELEVSRRKAKEQGADQFYYIGNHGMVKENI
STNSERKDV
>Mature_409_residues
MVPREIQLLQKPSGVNDNLPNRVEKFRLLENKILETFKLWGYDEVSVPYIEYSNIFSVNKENLLEQQMFQFNDETGRLVV
LRPDFTPSIARLVATYYKNHPLPLRLCYSGKIFRANNGNSINEKEQTQVGVELIGGEAPGGDAELVAMAAETFQKMEIKN
FVICIGNLKFINNLLDCLKVDQSSQSMLIEALNENNLVQYQGIIDSLELESDSKDILKKLPKFRGNKENLNYVREVAGFK
PVMNILDELENIFKTLESYGLQDKVTFDLSLVRKLDYYTGFIMEGYAEKVGFPLCGGGRYDKLMDKYGMSLPATGFAFSF
DSLIDLVKVKGDALSKLHFGYTQGKRDQALEHVKDYRQRGYRVSLELNSQELEVSRRKAKEQGADQFYYIGNHGMVKENI
STNSERKDV

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine [H]

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=350, Percent_Identity=22.2857142857143, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI15029520, Length=358, Percent_Identity=22.0670391061453, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1788861, Length=332, Percent_Identity=26.2048192771084, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6325290, Length=377, Percent_Identity=20.4244031830239, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24643061, Length=361, Percent_Identity=22.9916897506925, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24643059, Length=379, Percent_Identity=22.4274406332454, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR004517
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 46716; Mature: 46716

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPREIQLLQKPSGVNDNLPNRVEKFRLLENKILETFKLWGYDEVSVPYIEYSNIFSVNK
CCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEECCH
ENLLEQQMFQFNDETGRLVVLRPDFTPSIARLVATYYKNHPLPLRLCYSGKIFRANNGNS
HHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCC
INEKEQTQVGVELIGGEAPGGDAELVAMAAETFQKMEIKNFVICIGNLKFINNLLDCLKV
CCCHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHC
DQSSQSMLIEALNENNLVQYQGIIDSLELESDSKDILKKLPKFRGNKENLNYVREVAGFK
CCCHHHHHHHHHCCCCEEEEECCHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCHH
PVMNILDELENIFKTLESYGLQDKVTFDLSLVRKLDYYTGFIMEGYAEKVGFPLCGGGRY
HHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
DKLMDKYGMSLPATGFAFSFDSLIDLVKVKGDALSKLHFGYTQGKRDQALEHVKDYRQRG
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
YRVSLELNSQELEVSRRKAKEQGADQFYYIGNHGMVKENISTNSERKDV
EEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCCCCCCC
>Mature Secondary Structure
MVPREIQLLQKPSGVNDNLPNRVEKFRLLENKILETFKLWGYDEVSVPYIEYSNIFSVNK
CCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEECCH
ENLLEQQMFQFNDETGRLVVLRPDFTPSIARLVATYYKNHPLPLRLCYSGKIFRANNGNS
HHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCC
INEKEQTQVGVELIGGEAPGGDAELVAMAAETFQKMEIKNFVICIGNLKFINNLLDCLKV
CCCHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHC
DQSSQSMLIEALNENNLVQYQGIIDSLELESDSKDILKKLPKFRGNKENLNYVREVAGFK
CCCHHHHHHHHHCCCCEEEEECCHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCHH
PVMNILDELENIFKTLESYGLQDKVTFDLSLVRKLDYYTGFIMEGYAEKVGFPLCGGGRY
HHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
DKLMDKYGMSLPATGFAFSFDSLIDLVKVKGDALSKLHFGYTQGKRDQALEHVKDYRQRG
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
YRVSLELNSQELEVSRRKAKEQGADQFYYIGNHGMVKENISTNSERKDV
EEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA