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The map label for this gene is rocR [H]

Identifier: 188586463

GI number: 188586463

Start: 1926351

End: 1927625

Strand: Direct

Name: rocR [H]

Synonym: Nther_1848

Alternate gene names: 188586463

Gene position: 1926351-1927625 (Clockwise)

Preceding gene: 188586457

Following gene: 188586464

Centisome position: 60.85

GC content: 34.67

Gene sequence:

>1275_bases
GTGGGAAAGCATTTATTAGAAGTTTATCCATGGTTGACTGAGAAGACTAGTACAGCTTTAACGGTTTTAAGAACTGGACA
AGCCATTATTAATCAACCTCAAGTTATGGAACTCAGTGATGGGACAAATGTCAATTCAATAAATACGGCCTTCCCTCTCA
AAAATAAAGACGGAATCCTCGGAGTGGTTTTTTTATCAAACAATATCAACACTATTAACAGTACTTCGGGATCAAACTAC
ACCAAGCCAAAAAGACAGAGGCTCGGTGCCAAGTACACCTTTGACGATATAATTACTCAAAATGAAGGAATTTTAAATAT
TATTAACAAATTAAAAAAGGTTTCCAGGAATAATTCTAATATATTTATCTATGGTGAAACCGGAACAGGTAAAGAGCTAT
TTGTTCACGCAATACACAATGCCAGTAATCGAGCTAATAAGCCTTTTATATCTCAGAACTGTGCTGCTATTCCAGAATCT
TTGATGGAAAGTTTGATTTTTGGTACCACTGCAGGTAGTTTTACTGGCTCCACAGATAAAAAGGGTTTATTTGAACTGGC
AAACGGTGGGACAATATATTTGGATGAAATCAATTCTATGCCCTTAACACTTCAATCAAAACTATTAAGGGTTCTAGAAG
ATGGCAAGGTTCGTAGAATTGGGGGCAAAACCGAATATGAAACAGATGTCAGGATAATTGCCTCTACAAATGAAGCTCCC
GATTATTTGCTAAGAACTAATAAACTTAGAGAGGATTTATTTTACAGACTTAGTGTAGTCAATGTCGAAATTCCTCCTTT
AAGGGAACGAAAAGATGATATAGAATACCTATATGATTTTTTTATTAAAGATTTTAACGCTACTTTCGGAGAACATGTTG
ACGGAATAGATAGTCAAGTTAGACACATCTTTATGGACTATGATTGGCCCGGTAATATTAGAGAGTTAAAGAACTGCATA
GAAGGCGCCTTTAACAGTGTGGAAGGTACTATCATACAGTTAAAAGACATGCCCAGTTATATCAGTAAAAAAGCAAGATC
CTGTGCCGAAAATTCTAAAAACCAATGCGATAGTAACATTCAAGACCGAATCAATGAGAACTTTCTCTCTCACAATGAAT
TATCGTTAAAAAAGAATCAGGAAACCTTGGAGAAAAACTTGATAAAAAAAGCACTCAAGGAATCAAAAAACAATATTACT
CAAGCTGCTAAATTACTAGAAGTTCCGAGACAATCTGTTTATAATAAAATCAAAAAATACAATATTGAGCTATAG

Upstream 100 bases:

>100_bases
TAAAGAAACAGATTTAATTTGTATCTTAGATAGATATGGAAAAATACTTTATTATAATAATTATAACGATATTCATAATA
AATTAGGAACAGAAGATGTA

Downstream 100 bases:

>100_bases
AACAAATGAACAATTTGTGATGCAATATTTTAAATGATTGACTTCATATGTACAACTGTCAATCCCCTTGACAGTTGTAC
ATTTTTTTTACACCCACGTT

Product: putative sigma54 specific transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MGKHLLEVYPWLTEKTSTALTVLRTGQAIINQPQVMELSDGTNVNSINTAFPLKNKDGILGVVFLSNNINTINSTSGSNY
TKPKRQRLGAKYTFDDIITQNEGILNIINKLKKVSRNNSNIFIYGETGTGKELFVHAIHNASNRANKPFISQNCAAIPES
LMESLIFGTTAGSFTGSTDKKGLFELANGGTIYLDEINSMPLTLQSKLLRVLEDGKVRRIGGKTEYETDVRIIASTNEAP
DYLLRTNKLREDLFYRLSVVNVEIPPLRERKDDIEYLYDFFIKDFNATFGEHVDGIDSQVRHIFMDYDWPGNIRELKNCI
EGAFNSVEGTIIQLKDMPSYISKKARSCAENSKNQCDSNIQDRINENFLSHNELSLKKNQETLEKNLIKKALKESKNNIT
QAAKLLEVPRQSVYNKIKKYNIEL

Sequences:

>Translated_424_residues
MGKHLLEVYPWLTEKTSTALTVLRTGQAIINQPQVMELSDGTNVNSINTAFPLKNKDGILGVVFLSNNINTINSTSGSNY
TKPKRQRLGAKYTFDDIITQNEGILNIINKLKKVSRNNSNIFIYGETGTGKELFVHAIHNASNRANKPFISQNCAAIPES
LMESLIFGTTAGSFTGSTDKKGLFELANGGTIYLDEINSMPLTLQSKLLRVLEDGKVRRIGGKTEYETDVRIIASTNEAP
DYLLRTNKLREDLFYRLSVVNVEIPPLRERKDDIEYLYDFFIKDFNATFGEHVDGIDSQVRHIFMDYDWPGNIRELKNCI
EGAFNSVEGTIIQLKDMPSYISKKARSCAENSKNQCDSNIQDRINENFLSHNELSLKKNQETLEKNLIKKALKESKNNIT
QAAKLLEVPRQSVYNKIKKYNIEL
>Mature_423_residues
GKHLLEVYPWLTEKTSTALTVLRTGQAIINQPQVMELSDGTNVNSINTAFPLKNKDGILGVVFLSNNINTINSTSGSNYT
KPKRQRLGAKYTFDDIITQNEGILNIINKLKKVSRNNSNIFIYGETGTGKELFVHAIHNASNRANKPFISQNCAAIPESL
MESLIFGTTAGSFTGSTDKKGLFELANGGTIYLDEINSMPLTLQSKLLRVLEDGKVRRIGGKTEYETDVRIIASTNEAPD
YLLRTNKLREDLFYRLSVVNVEIPPLRERKDDIEYLYDFFIKDFNATFGEHVDGIDSQVRHIFMDYDWPGNIRELKNCIE
GAFNSVEGTIIQLKDMPSYISKKARSCAENSKNQCDSNIQDRINENFLSHNELSLKKNQETLEKNLIKKALKESKNNITQ
AAKLLEVPRQSVYNKIKKYNIEL

Specific function: Positive regulator of arginine catabolism. Controls the transcription of the two operons rocABC and rocDEF and probably acts by binding to the corresponding upstream activating sequences [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=339, Percent_Identity=38.0530973451327, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1790299, Length=331, Percent_Identity=36.2537764350453, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1789233, Length=332, Percent_Identity=38.855421686747, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI87082117, Length=240, Percent_Identity=42.9166666666667, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI1789087, Length=242, Percent_Identity=41.3223140495868, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI1788905, Length=328, Percent_Identity=34.1463414634146, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1790437, Length=324, Percent_Identity=32.7160493827161, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI1786524, Length=262, Percent_Identity=40.4580152671756, Blast_Score=201, Evalue=6e-53,
Organism=Escherichia coli, GI1787583, Length=332, Percent_Identity=32.8313253012048, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI87082152, Length=328, Percent_Identity=32.6219512195122, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI87081872, Length=330, Percent_Identity=33.3333333333333, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI87081858, Length=333, Percent_Identity=27.027027027027, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1789828, Length=255, Percent_Identity=27.843137254902, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF08448 PAS_4; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 47921; Mature: 47790

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKHLLEVYPWLTEKTSTALTVLRTGQAIINQPQVMELSDGTNVNSINTAFPLKNKDGIL
CCCCHHHHHCCCCCCCHHHHHHHCCCHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCEE
GVVFLSNNINTINSTSGSNYTKPKRQRLGAKYTFDDIITQNEGILNIINKLKKVSRNNSN
EEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCE
IFIYGETGTGKELFVHAIHNASNRANKPFISQNCAAIPESLMESLIFGTTAGSFTGSTDK
EEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
KGLFELANGGTIYLDEINSMPLTLQSKLLRVLEDGKVRRIGGKTEYETDVRIIASTNEAP
CCHHEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEECCCCC
DYLLRTNKLREDLFYRLSVVNVEIPPLRERKDDIEYLYDFFIKDFNATFGEHVDGIDSQV
HHHHHHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCHHHHH
RHIFMDYDWPGNIRELKNCIEGAFNSVEGTIIQLKDMPSYISKKARSCAENSKNQCDSNI
HEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCHHHCCCHH
QDRINENFLSHNELSLKKNQETLEKNLIKKALKESKNNITQAAKLLEVPRQSVYNKIKKY
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIEL
CCCC
>Mature Secondary Structure 
GKHLLEVYPWLTEKTSTALTVLRTGQAIINQPQVMELSDGTNVNSINTAFPLKNKDGIL
CCCHHHHHCCCCCCCHHHHHHHCCCHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCEE
GVVFLSNNINTINSTSGSNYTKPKRQRLGAKYTFDDIITQNEGILNIINKLKKVSRNNSN
EEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCE
IFIYGETGTGKELFVHAIHNASNRANKPFISQNCAAIPESLMESLIFGTTAGSFTGSTDK
EEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
KGLFELANGGTIYLDEINSMPLTLQSKLLRVLEDGKVRRIGGKTEYETDVRIIASTNEAP
CCHHEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEECCCCC
DYLLRTNKLREDLFYRLSVVNVEIPPLRERKDDIEYLYDFFIKDFNATFGEHVDGIDSQV
HHHHHHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCHHHHH
RHIFMDYDWPGNIRELKNCIEGAFNSVEGTIIQLKDMPSYISKKARSCAENSKNQCDSNI
HEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCHHHCCCHH
QDRINENFLSHNELSLKKNQETLEKNLIKKALKESKNNITQAAKLLEVPRQSVYNKIKKY
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIEL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113162; 9384377 [H]