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The map label for this gene is rnhB

Identifier: 188586001

GI number: 188586001

Start: 1445019

End: 1445615

Strand: Direct

Name: rnhB

Synonym: Nther_1375

Alternate gene names: 188586001

Gene position: 1445019-1445615 (Clockwise)

Preceding gene: 188586000

Following gene: 188586002

Centisome position: 45.65

GC content: 35.51

Gene sequence:

>597_bases
ATGATGTATTATTGTGGTATTGATGAAGCTGGTTTCGGAGCAGGAGCTGCCCAGGTTTATGTATCAGCCTGTGTTTTAGA
TCCACAGAAACCTATTAATGAAAGCCTAACGGATTCTAAGCAATTGACTCCTAAAAAAAGGGAACTTCTTGCAGAAGAAA
TTAAACAAGACGCTTTAACGTGGTGTATAGCTACTGCAAGTGTGGAAGAAATCGAAAAACTAAATATTAGAAAAGCTACG
ATTTTGGCAATGAAGCGTTCTCTTGAAGGATTAACTGTTAAACCAGATATGGTTTACATAGATGGCATTAATTCACCTGA
AGTGCCTTTTCCAGTGGAAACTGTCGTTAAAGGTGATAGTAAAATCCCTGCCATAAGTGCAGCTTCAATTCTAGCCAAAG
TTGCCCGTGATAATAGGATGCTAGAATATGATGAACAATATCCTGAATACGGATTTAAAGACCATAAAGGTTATCTGACT
AAAAACCATATCAAAGCTATTCAAAAATACGGACCCAGTCCAATACATAGAAAAACATATAAACCTATTAAAAAAATTAT
TGATAATCAGAAAAATCAACAGTTGGAGCTATTTTAG

Upstream 100 bases:

>100_bases
GGGAAAAGCTTTTTTAATCTTTTGGCCTTTAGATGATGTTGGGAGTATATAAGATGCTTTTGGTATATATTTTGAGTTGT
TCTATATGTCGGAGGGAATT

Downstream 100 bases:

>100_bases
GGAATTATTATGATTGTGATTAAGTATTAATTAAAATGAAAAGAGGCACACAAGCAGGTGAACAATAAATCCAAGGGAAG
AACTGCCGAAAAGATTGCAA

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MMYYCGIDEAGFGAGAAQVYVSACVLDPQKPINESLTDSKQLTPKKRELLAEEIKQDALTWCIATASVEEIEKLNIRKAT
ILAMKRSLEGLTVKPDMVYIDGINSPEVPFPVETVVKGDSKIPAISAASILAKVARDNRMLEYDEQYPEYGFKDHKGYLT
KNHIKAIQKYGPSPIHRKTYKPIKKIIDNQKNQQLELF

Sequences:

>Translated_198_residues
MMYYCGIDEAGFGAGAAQVYVSACVLDPQKPINESLTDSKQLTPKKRELLAEEIKQDALTWCIATASVEEIEKLNIRKAT
ILAMKRSLEGLTVKPDMVYIDGINSPEVPFPVETVVKGDSKIPAISAASILAKVARDNRMLEYDEQYPEYGFKDHKGYLT
KNHIKAIQKYGPSPIHRKTYKPIKKIIDNQKNQQLELF
>Mature_198_residues
MMYYCGIDEAGFGAGAAQVYVSACVLDPQKPINESLTDSKQLTPKKRELLAEEIKQDALTWCIATASVEEIEKLNIRKAT
ILAMKRSLEGLTVKPDMVYIDGINSPEVPFPVETVVKGDSKIPAISAASILAKVARDNRMLEYDEQYPEYGFKDHKGYLT
KNHIKAIQKYGPSPIHRKTYKPIKKIIDNQKNQQLELF

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=183, Percent_Identity=46.448087431694, Blast_Score=177, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_NATTJ (B2A2P0)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917546.1
- ProteinModelPortal:   B2A2P0
- SMR:   B2A2P0
- GeneID:   6315688
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1375
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 22263; Mature: 22263

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMYYCGIDEAGFGAGAAQVYVSACVLDPQKPINESLTDSKQLTPKKRELLAEEIKQDALT
CEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHH
WCIATASVEEIEKLNIRKATILAMKRSLEGLTVKPDMVYIDGINSPEVPFPVETVVKGDS
HHHHHHCHHHHHHHCCHHHHHHHHHHHCCCCEECCCEEEEECCCCCCCCCCHHHHHCCCC
KIPAISAASILAKVARDNRMLEYDEQYPEYGFKDHKGYLTKNHIKAIQKYGPSPIHRKTY
CCCHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHH
KPIKKIIDNQKNQQLELF
HHHHHHHCCCCCCEEECC
>Mature Secondary Structure
MMYYCGIDEAGFGAGAAQVYVSACVLDPQKPINESLTDSKQLTPKKRELLAEEIKQDALT
CEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHH
WCIATASVEEIEKLNIRKATILAMKRSLEGLTVKPDMVYIDGINSPEVPFPVETVVKGDS
HHHHHHCHHHHHHHCCHHHHHHHHHHHCCCCEECCCEEEEECCCCCCCCCCHHHHHCCCC
KIPAISAASILAKVARDNRMLEYDEQYPEYGFKDHKGYLTKNHIKAIQKYGPSPIHRKTY
CCCHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHH
KPIKKIIDNQKNQQLELF
HHHHHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA