Click here to switch to the map view.

The map label for this gene is def

Identifier: 188585959

GI number: 188585959

Start: 1407459

End: 1407929

Strand: Direct

Name: def

Synonym: Nther_1333

Alternate gene names: 188585959

Gene position: 1407459-1407929 (Clockwise)

Preceding gene: 188585958

Following gene: 188585960

Centisome position: 44.46

GC content: 37.37

Gene sequence:

>471_bases
TTGGCGATTAAAAAAATTAGAACTAATGATGATCCTGTACTTAAACGGAAGGCCAAAAAAGTTACAAATATAGATGATAG
ATTGGAAAGACTGTTAACAAACATGCTTGATACCATGTATGAAGCTGAAGGAATTGGCCTAGCAGCACCACAAATAGGAA
TATCAAAAAGAGTGATTGTTGTTGATATAGGTGAAGATGAAATTTATCAGTTAATAAACCCAGAAATAGTTGATACTTCC
GATGAGCAAGAAAAAGCCTTGGAAGGTTGTTTGAGTTATCCTGGATTACAGGGGCGGGTCACTAGACCTGTTAAAGTTAC
AGTGAAAGCTCTGAATCCCCAGGAAGAAGAAATGATTATAGAAGCAGAGGGATTATTGGCCCGGGCTTTACAGCACGAAA
TAGATCATTTGGATGGTATTACTTTTATTGACAGGGCAGAAGAAGTGTTTAGGGAAGAGGAATCTCATTAA

Upstream 100 bases:

>100_bases
ATAAGAATGGCAATTGATGTAGATCCTTTAAACTTACTATAATATAGGCTGTAAAAAAAGCTTTACAGAGTAGTTTGCAT
CTTTGGAGGTGGCAATAATA

Downstream 100 bases:

>100_bases
ATTGAGGTGGTATATTTATGCGAACTATATTCATGGGTACACCAGACTTTTCAGTTCCTTTTATTGAGGCAATTGCTCGA
AGCACCCACAATTTGAACTT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MAIKKIRTNDDPVLKRKAKKVTNIDDRLERLLTNMLDTMYEAEGIGLAAPQIGISKRVIVVDIGEDEIYQLINPEIVDTS
DEQEKALEGCLSYPGLQGRVTRPVKVTVKALNPQEEEMIIEAEGLLARALQHEIDHLDGITFIDRAEEVFREEESH

Sequences:

>Translated_156_residues
MAIKKIRTNDDPVLKRKAKKVTNIDDRLERLLTNMLDTMYEAEGIGLAAPQIGISKRVIVVDIGEDEIYQLINPEIVDTS
DEQEKALEGCLSYPGLQGRVTRPVKVTVKALNPQEEEMIIEAEGLLARALQHEIDHLDGITFIDRAEEVFREEESH
>Mature_155_residues
AIKKIRTNDDPVLKRKAKKVTNIDDRLERLLTNMLDTMYEAEGIGLAAPQIGISKRVIVVDIGEDEIYQLINPEIVDTSD
EQEKALEGCLSYPGLQGRVTRPVKVTVKALNPQEEEMIIEAEGLLARALQHEIDHLDGITFIDRAEEVFREEESH

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=156, Percent_Identity=30.1282051282051, Blast_Score=74, Evalue=5e-14,
Organism=Escherichia coli, GI1789682, Length=146, Percent_Identity=41.7808219178082, Blast_Score=105, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24645728, Length=156, Percent_Identity=33.974358974359, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24645726, Length=155, Percent_Identity=28.3870967741935, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_NATTJ (B2A2K1)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917504.1
- ProteinModelPortal:   B2A2K1
- SMR:   B2A2K1
- GeneID:   6314443
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1333
- HOGENOM:   HBG665227
- OMA:   EFLAIVM
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 17621; Mature: 17490

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKKIRTNDDPVLKRKAKKVTNIDDRLERLLTNMLDTMYEAEGIGLAAPQIGISKRVIV
CCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
VDIGEDEIYQLINPEIVDTSDEQEKALEGCLSYPGLQGRVTRPVKVTVKALNPQEEEMII
EECCHHHHHHHCCCCEECCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHEEE
EAEGLLARALQHEIDHLDGITFIDRAEEVFREEESH
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIKKIRTNDDPVLKRKAKKVTNIDDRLERLLTNMLDTMYEAEGIGLAAPQIGISKRVIV
CCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
VDIGEDEIYQLINPEIVDTSDEQEKALEGCLSYPGLQGRVTRPVKVTVKALNPQEEEMII
EECCHHHHHHHCCCCEECCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHEEE
EAEGLLARALQHEIDHLDGITFIDRAEEVFREEESH
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA