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The map label for this gene is priA [H]

Identifier: 188585958

GI number: 188585958

Start: 1405151

End: 1407400

Strand: Direct

Name: priA [H]

Synonym: Nther_1332

Alternate gene names: 188585958

Gene position: 1405151-1407400 (Clockwise)

Preceding gene: 188585957

Following gene: 188585959

Centisome position: 44.39

GC content: 36.27

Gene sequence:

>2250_bases
ATGAAATACTGTCATGTAACAATTCTAGATATCGTATCCGATGAATTGGACAGGCAATTCACATATAAAATAACACCACA
TTTGGAATCTAGGGTCACTCGGGGTTCTTTAGTTAAAGTTCCTTTTGGTAAGAGAAATGTTAATGCAGTTGTCAATGAAG
TGACTGGAACTACTTCTTTATCCAAAGATAAGATAAAAAACGTGAATCAAGTCCTATCAGATCAACCTCTCCCGGAAGAT
ATCATGGAACTTGCCCAGTGGATGAGCAGCTATTATTTATGTTCGCTGTCTAAAGCTTACAATATTATGGTTCCCCAGGG
GTTAAAATCAGGAGTGAATCGCATTTATAAAAAATACGTGTCTATAGCAGATATAACTGAAGCTAGAAAATTACTAGACA
GTGCTAAGGCACCTAAACAAAGGAAAGTTCTTGAACGCTTACTTAGTAATTCACCCTTAGCTCTTGATCAGTTACTAGAT
GAGTTAAAAATCACAAAATCCCCGGTAGAATCTCTTTATGGGAAGGGAATAATTAATATCCAAAGTAGTCGATCCTATAG
GGAACCCATTGATTTCAAACAAATCGAAGAGTATAAACCTAAAGAACCTACACAGGCCCAAAAAACTGTGCTAAAATTAA
TCAAGAATCAGGGTAGAGGTAGTTGTGATAACACTTCTCCTAAGAAGCCGAAGCCTTTTTTACTATTTGGGGTAACCGGG
AGTGGTAAAACTGAAATTTATCTTCAATTGATTGAATCGTATTTACAGCAAGGACTGCAGACGATAGTATTGATACCCGA
AATCTCCCTAACTCCCCAAACAATTGAACGGTTTGTTGGAAGATTTGGAAACCAAGTGGCTGTTACACACAGTAAATTGA
GTCATGGGGAAAGATTAGATCAATGGGAGAAAATGTTGTGGGGTAAAGCTAATATAGTTGTGGGGCCTCGATCTGCTGTA
TTTGCACCTTTTTCAAATCTCGGGTTAATTATTATCGATGAAGAGCAAGAAGGCTCCTACAAACAAGAAGAATTGCCCCG
GTACCATGCTAGGGAGGTAGCTGAACGAAGATGTCAGTTTAAAGATGGTCAATTGTTACTTGGAACAGCTACTCCATCTT
TAGAAAGTATACACCGAGTCAAGTCAGATGATTACAATTTGGCAGAATTACCTGAGAGAGTAGGAGATGTTGAACTTCCC
GATATATCTGTGATTGACATGAAAGAAGAATTTCAGAAGGGCAATAAGTCGGTATTCAGTAGAGAATTAATATTAAAGCT
AAAAGAAGTTACAGATAAAGGCGAACAGACGATTTTATTTTTAAATAGAAGAGGGTATTCTACATTTCTACTCTGTAGAG
AATGTGGCTATACTGTAACCTGTCCGAGATGTGAAGTTACTCTGACATACCATAAATCAATTAATCAATTGTTGTGTCAT
TATTGTGATTACACAGAACCCCTATCAAAATTTTGTCCGGATTGTAATAGCGATAAAATCAAAGAATTTGGTACAGGAAC
TCAAAAAATAGAAAGAGAACTAAAAAAATATTTACCTGATTTAAAAACTATTAGAATGGATGTGGATACAACTCGGAAAA
AGAACTCCCATCAAGAATTATTGGGAAAGTTCAAAAATAAACAGGCAAATGTGCTAATTGGCACTCAAATGATAGCTAAG
GGTCTTGATTTCCCTGACGTTTCTCTTGTTGGTATAATTACAGCAGACACGGCTCTTAATTTGCCAGATTTTCGAGCTGG
TGAAAAAACATTTCAATTATTAACTCAAGTAGCTGGAAGGGCAGGACGCAGAGACAATAATCAGCGAGGGCAAGTAGTAA
TACAAACTTATAATCCAGATCATTACAGCATTGAAGCAGTTAAAAATGAAAATGTGCGTAAATTTACCGGGGAAGAACTT
GCTTTACGTAAAAAATTAGGTTATCCTCCATTTAAGAGAATGATCAGGATTATGTTAAGGTCTCCTTATGAACAGGCTGT
ATCCCATCAGGTAGAGGAACTATCACAAAAATTGCAGGATATGGGCTATGAAGTGTTAGGTCCTACCCCTTGTCCCATAC
CTAAAATCAAGGATGAATTTAGATGGCATCTAATGTTAAGGCTTGATCTAAATAATCAAGAAATAATAGATGAACAACTT
CGAGATTACTTGCTTACAACAAAAAATAATTTATCTCAACAGGGAAAAATAAGAATGGCAATTGATGTAGATCCTTTAAA
CTTACTATAA

Upstream 100 bases:

>100_bases
GAATGTGGATAGTTGAATATTGATAACTGTAAATATTACAGTATCAACAACTAAATATAACAGCACCAATGTTAAAAACT
GGGGGAGGTTTTAAGGGTTT

Downstream 100 bases:

>100_bases
TATAGGCTGTAAAAAAAGCTTTACAGAGTAGTTTGCATCTTTGGAGGTGGCAATAATATTGGCGATTAAAAAAATTAGAA
CTAATGATGATCCTGTACTT

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 749; Mature: 749

Protein sequence:

>749_residues
MKYCHVTILDIVSDELDRQFTYKITPHLESRVTRGSLVKVPFGKRNVNAVVNEVTGTTSLSKDKIKNVNQVLSDQPLPED
IMELAQWMSSYYLCSLSKAYNIMVPQGLKSGVNRIYKKYVSIADITEARKLLDSAKAPKQRKVLERLLSNSPLALDQLLD
ELKITKSPVESLYGKGIINIQSSRSYREPIDFKQIEEYKPKEPTQAQKTVLKLIKNQGRGSCDNTSPKKPKPFLLFGVTG
SGKTEIYLQLIESYLQQGLQTIVLIPEISLTPQTIERFVGRFGNQVAVTHSKLSHGERLDQWEKMLWGKANIVVGPRSAV
FAPFSNLGLIIIDEEQEGSYKQEELPRYHAREVAERRCQFKDGQLLLGTATPSLESIHRVKSDDYNLAELPERVGDVELP
DISVIDMKEEFQKGNKSVFSRELILKLKEVTDKGEQTILFLNRRGYSTFLLCRECGYTVTCPRCEVTLTYHKSINQLLCH
YCDYTEPLSKFCPDCNSDKIKEFGTGTQKIERELKKYLPDLKTIRMDVDTTRKKNSHQELLGKFKNKQANVLIGTQMIAK
GLDFPDVSLVGIITADTALNLPDFRAGEKTFQLLTQVAGRAGRRDNNQRGQVVIQTYNPDHYSIEAVKNENVRKFTGEEL
ALRKKLGYPPFKRMIRIMLRSPYEQAVSHQVEELSQKLQDMGYEVLGPTPCPIPKIKDEFRWHLMLRLDLNNQEIIDEQL
RDYLLTTKNNLSQQGKIRMAIDVDPLNLL

Sequences:

>Translated_749_residues
MKYCHVTILDIVSDELDRQFTYKITPHLESRVTRGSLVKVPFGKRNVNAVVNEVTGTTSLSKDKIKNVNQVLSDQPLPED
IMELAQWMSSYYLCSLSKAYNIMVPQGLKSGVNRIYKKYVSIADITEARKLLDSAKAPKQRKVLERLLSNSPLALDQLLD
ELKITKSPVESLYGKGIINIQSSRSYREPIDFKQIEEYKPKEPTQAQKTVLKLIKNQGRGSCDNTSPKKPKPFLLFGVTG
SGKTEIYLQLIESYLQQGLQTIVLIPEISLTPQTIERFVGRFGNQVAVTHSKLSHGERLDQWEKMLWGKANIVVGPRSAV
FAPFSNLGLIIIDEEQEGSYKQEELPRYHAREVAERRCQFKDGQLLLGTATPSLESIHRVKSDDYNLAELPERVGDVELP
DISVIDMKEEFQKGNKSVFSRELILKLKEVTDKGEQTILFLNRRGYSTFLLCRECGYTVTCPRCEVTLTYHKSINQLLCH
YCDYTEPLSKFCPDCNSDKIKEFGTGTQKIERELKKYLPDLKTIRMDVDTTRKKNSHQELLGKFKNKQANVLIGTQMIAK
GLDFPDVSLVGIITADTALNLPDFRAGEKTFQLLTQVAGRAGRRDNNQRGQVVIQTYNPDHYSIEAVKNENVRKFTGEEL
ALRKKLGYPPFKRMIRIMLRSPYEQAVSHQVEELSQKLQDMGYEVLGPTPCPIPKIKDEFRWHLMLRLDLNNQEIIDEQL
RDYLLTTKNNLSQQGKIRMAIDVDPLNLL
>Mature_749_residues
MKYCHVTILDIVSDELDRQFTYKITPHLESRVTRGSLVKVPFGKRNVNAVVNEVTGTTSLSKDKIKNVNQVLSDQPLPED
IMELAQWMSSYYLCSLSKAYNIMVPQGLKSGVNRIYKKYVSIADITEARKLLDSAKAPKQRKVLERLLSNSPLALDQLLD
ELKITKSPVESLYGKGIINIQSSRSYREPIDFKQIEEYKPKEPTQAQKTVLKLIKNQGRGSCDNTSPKKPKPFLLFGVTG
SGKTEIYLQLIESYLQQGLQTIVLIPEISLTPQTIERFVGRFGNQVAVTHSKLSHGERLDQWEKMLWGKANIVVGPRSAV
FAPFSNLGLIIIDEEQEGSYKQEELPRYHAREVAERRCQFKDGQLLLGTATPSLESIHRVKSDDYNLAELPERVGDVELP
DISVIDMKEEFQKGNKSVFSRELILKLKEVTDKGEQTILFLNRRGYSTFLLCRECGYTVTCPRCEVTLTYHKSINQLLCH
YCDYTEPLSKFCPDCNSDKIKEFGTGTQKIERELKKYLPDLKTIRMDVDTTRKKNSHQELLGKFKNKQANVLIGTQMIAK
GLDFPDVSLVGIITADTALNLPDFRAGEKTFQLLTQVAGRAGRRDNNQRGQVVIQTYNPDHYSIEAVKNENVRKFTGEEL
ALRKKLGYPPFKRMIRIMLRSPYEQAVSHQVEELSQKLQDMGYEVLGPTPCPIPKIKDEFRWHLMLRLDLNNQEIIDEQL
RDYLLTTKNNLSQQGKIRMAIDVDPLNLL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=743, Percent_Identity=33.7819650067295, Blast_Score=414, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 85501; Mature: 85501

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYCHVTILDIVSDELDRQFTYKITPHLESRVTRGSLVKVPFGKRNVNAVVNEVTGTTSL
CCEEEEEHHHHHHHHHCCEEEEEECCCHHHHCCCCCEEECCCCCCCHHHHHHHHCCCCCC
SKDKIKNVNQVLSDQPLPEDIMELAQWMSSYYLCSLSKAYNIMVPQGLKSGVNRIYKKYV
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHH
SIADITEARKLLDSAKAPKQRKVLERLLSNSPLALDQLLDELKITKSPVESLYGKGIINI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
QSSRSYREPIDFKQIEEYKPKEPTQAQKTVLKLIKNQGRGSCDNTSPKKPKPFLLFGVTG
ECCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECC
SGKTEIYLQLIESYLQQGLQTIVLIPEISLTPQTIERFVGRFGNQVAVTHSKLSHGERLD
CCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEHHHHHCCHHHH
QWEKMLWGKANIVVGPRSAVFAPFSNLGLIIIDEEQEGSYKQEELPRYHAREVAERRCQF
HHHHHHCCCCEEEECCCCCEECCCCCCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHCCC
KDGQLLLGTATPSLESIHRVKSDDYNLAELPERVGDVELPDISVIDMKEEFQKGNKSVFS
CCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEECCHHHHCCCCHHHHH
RELILKLKEVTDKGEQTILFLNRRGYSTFLLCRECGYTVTCPRCEVTLTYHKSINQLLCH
HHHHHHHHHHCCCCCEEEEEEECCCCCEEEEHHHCCCEEECCCEEEEEEHHHHHHHHHHH
YCDYTEPLSKFCPDCNSDKIKEFGTGTQKIERELKKYLPDLKTIRMDVDTTRKKNSHQEL
HCCCCCHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHCCCHHEEEECCHHHCCCCCHHHH
LGKFKNKQANVLIGTQMIAKGLDFPDVSLVGIITADTALNLPDFRAGEKTFQLLTQVAGR
HHHHCCCCCCEEEEHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
AGRRDNNQRGQVVIQTYNPDHYSIEAVKNENVRKFTGEELALRKKLGYPPFKRMIRIMLR
CCCCCCCCCCCEEEEEECCCCEEEEEECCCCCHHHCCHHHHHHHHCCCCHHHHHHHHHHH
SPYEQAVSHQVEELSQKLQDMGYEVLGPTPCPIPKIKDEFRWHLMLRLDLNNQEIIDEQL
CCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHH
RDYLLTTKNNLSQQGKIRMAIDVDPLNLL
HHHHHHCCCCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MKYCHVTILDIVSDELDRQFTYKITPHLESRVTRGSLVKVPFGKRNVNAVVNEVTGTTSL
CCEEEEEHHHHHHHHHCCEEEEEECCCHHHHCCCCCEEECCCCCCCHHHHHHHHCCCCCC
SKDKIKNVNQVLSDQPLPEDIMELAQWMSSYYLCSLSKAYNIMVPQGLKSGVNRIYKKYV
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHH
SIADITEARKLLDSAKAPKQRKVLERLLSNSPLALDQLLDELKITKSPVESLYGKGIINI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
QSSRSYREPIDFKQIEEYKPKEPTQAQKTVLKLIKNQGRGSCDNTSPKKPKPFLLFGVTG
ECCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECC
SGKTEIYLQLIESYLQQGLQTIVLIPEISLTPQTIERFVGRFGNQVAVTHSKLSHGERLD
CCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEHHHHHCCHHHH
QWEKMLWGKANIVVGPRSAVFAPFSNLGLIIIDEEQEGSYKQEELPRYHAREVAERRCQF
HHHHHHCCCCEEEECCCCCEECCCCCCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHCCC
KDGQLLLGTATPSLESIHRVKSDDYNLAELPERVGDVELPDISVIDMKEEFQKGNKSVFS
CCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEECCHHHHCCCCHHHHH
RELILKLKEVTDKGEQTILFLNRRGYSTFLLCRECGYTVTCPRCEVTLTYHKSINQLLCH
HHHHHHHHHHCCCCCEEEEEEECCCCCEEEEHHHCCCEEECCCEEEEEEHHHHHHHHHHH
YCDYTEPLSKFCPDCNSDKIKEFGTGTQKIERELKKYLPDLKTIRMDVDTTRKKNSHQEL
HCCCCCHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHCCCHHEEEECCHHHCCCCCHHHH
LGKFKNKQANVLIGTQMIAKGLDFPDVSLVGIITADTALNLPDFRAGEKTFQLLTQVAGR
HHHHCCCCCCEEEEHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
AGRRDNNQRGQVVIQTYNPDHYSIEAVKNENVRKFTGEELALRKKLGYPPFKRMIRIMLR
CCCCCCCCCCCEEEEEECCCCEEEEEECCCCCHHHCCHHHHHHHHCCCCHHHHHHHHHHH
SPYEQAVSHQVEELSQKLQDMGYEVLGPTPCPIPKIKDEFRWHLMLRLDLNNQEIIDEQL
CCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHH
RDYLLTTKNNLSQQGKIRMAIDVDPLNLL
HHHHHHCCCCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]