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The map label for this gene is fmt

Identifier: 188585960

GI number: 188585960

Start: 1407947

End: 1408888

Strand: Direct

Name: fmt

Synonym: Nther_1334

Alternate gene names: 188585960

Gene position: 1407947-1408888 (Clockwise)

Preceding gene: 188585959

Following gene: 188585961

Centisome position: 44.48

GC content: 37.79

Gene sequence:

>942_bases
ATGCGAACTATATTCATGGGTACACCAGACTTTTCAGTTCCTTTTATTGAGGCAATTGCTCGAAGCACCCACAATTTGAA
CTTAGTTGTAACACAACCAGATAGAAGAAAAGGTCGGGGGAAGGAATTGCAACCACCTCCGGCTAAAAGAAAAGCTGAGG
AGCTTGGAATAGATGTATTTCAACCCGAAAGCATTCACAATAATTATGCTTATCAGATTTTATCCGATATTGAACCCCAT
TTAATAGTCACCGCAGCTTATGGACAAATCTTACCCAGAAAAATATTGGATTTACCTCGGATAAAAGCTATTAATGTCCA
TGCCTCACTGTTACCGGAATACAGGGGAGCAGCACCTATTCATCGAGCTGTTATGGACGGCAAGGAGCAAACCGGGGTGA
CTATTATGGAAATGTGCGATAAAATGGACGCAGGGGATATACTAAATTATGAATCGGTAGATATTGGAAAAACAGACACC
ACAGGAGATGTATATAAACAAATCATAACAGTTGGCCCCCAACTGCTAATAGAAACTATGGACTTACTGGAAAAAAATCA
AGTAACTCCTTTAAAACAAGATGAAAATCAAGTTTCTTATGCACCTAAATTAAAAAAAGAAGATGAATACCTGGATTTTT
CTAAATATACCAATACAGAAGTGTTTAATAGAGTTAGGGGGTTGAACCCCTGGCCTGGAGCCTTTACTAAGTTTGAAGGC
AAACGCCTAAAAATATGGGAGACGAAAGTTCACAATAGTAGTTCATTTAATTCAAACTCTAAACCAGGTGAAATTATTGA
GATAAACCAACAAGGACCTGTGGTTAAATGTTGTCAAGGTTCTGTCATATTAACAAAAATTCAACCATCCGGAAAAAAAG
CAATGACTGGTGAACAGTTTATTCGGGGCTATGATATAAAATCAGGGATACAACTGGAATAA

Upstream 100 bases:

>100_bases
TACAGCACGAAATAGATCATTTGGATGGTATTACTTTTATTGACAGGGCAGAAGAAGTGTTTAGGGAAGAGGAATCTCAT
TAAATTGAGGTGGTATATTT

Downstream 100 bases:

>100_bases
GGAAGTGATAAAATTATGGAAGCGAGGAAAAGATTATTTCTCGGTTTAGTTAGTTTAACTATTGTTTTAAGTGGTTTGTT
GTTGCTGTATTTAGGTCAGC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MRTIFMGTPDFSVPFIEAIARSTHNLNLVVTQPDRRKGRGKELQPPPAKRKAEELGIDVFQPESIHNNYAYQILSDIEPH
LIVTAAYGQILPRKILDLPRIKAINVHASLLPEYRGAAPIHRAVMDGKEQTGVTIMEMCDKMDAGDILNYESVDIGKTDT
TGDVYKQIITVGPQLLIETMDLLEKNQVTPLKQDENQVSYAPKLKKEDEYLDFSKYTNTEVFNRVRGLNPWPGAFTKFEG
KRLKIWETKVHNSSSFNSNSKPGEIIEINQQGPVVKCCQGSVILTKIQPSGKKAMTGEQFIRGYDIKSGIQLE

Sequences:

>Translated_313_residues
MRTIFMGTPDFSVPFIEAIARSTHNLNLVVTQPDRRKGRGKELQPPPAKRKAEELGIDVFQPESIHNNYAYQILSDIEPH
LIVTAAYGQILPRKILDLPRIKAINVHASLLPEYRGAAPIHRAVMDGKEQTGVTIMEMCDKMDAGDILNYESVDIGKTDT
TGDVYKQIITVGPQLLIETMDLLEKNQVTPLKQDENQVSYAPKLKKEDEYLDFSKYTNTEVFNRVRGLNPWPGAFTKFEG
KRLKIWETKVHNSSSFNSNSKPGEIIEINQQGPVVKCCQGSVILTKIQPSGKKAMTGEQFIRGYDIKSGIQLE
>Mature_313_residues
MRTIFMGTPDFSVPFIEAIARSTHNLNLVVTQPDRRKGRGKELQPPPAKRKAEELGIDVFQPESIHNNYAYQILSDIEPH
LIVTAAYGQILPRKILDLPRIKAINVHASLLPEYRGAAPIHRAVMDGKEQTGVTIMEMCDKMDAGDILNYESVDIGKTDT
TGDVYKQIITVGPQLLIETMDLLEKNQVTPLKQDENQVSYAPKLKKEDEYLDFSKYTNTEVFNRVRGLNPWPGAFTKFEG
KRLKIWETKVHNSSSFNSNSKPGEIIEINQQGPVVKCCQGSVILTKIQPSGKKAMTGEQFIRGYDIKSGIQLE

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI238814322, Length=274, Percent_Identity=24.8175182481752, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI21614513, Length=266, Percent_Identity=24.4360902255639, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI164663775, Length=320, Percent_Identity=26.25, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1789683, Length=301, Percent_Identity=42.1926910299003, Blast_Score=254, Evalue=6e-69,
Organism=Escherichia coli, GI1788589, Length=313, Percent_Identity=25.5591054313099, Blast_Score=135, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI133930964, Length=327, Percent_Identity=24.4648318042813, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6319458, Length=361, Percent_Identity=24.9307479224377, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45550868, Length=306, Percent_Identity=28.1045751633987, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28571984, Length=225, Percent_Identity=30.6666666666667, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24585660, Length=147, Percent_Identity=27.2108843537415, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_NATTJ (B2A2K2)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917505.1
- ProteinModelPortal:   B2A2K2
- SMR:   B2A2K2
- GeneID:   6314444
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1334
- HOGENOM:   HBG571560
- OMA:   VVAYGAI
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 35186; Mature: 35186

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTIFMGTPDFSVPFIEAIARSTHNLNLVVTQPDRRKGRGKELQPPPAKRKAEELGIDVF
CCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCHHHCCCCCCCCCCCHHHHHHHCCCEEE
QPESIHNNYAYQILSDIEPHLIVTAAYGQILPRKILDLPRIKAINVHASLLPEYRGAAPI
CCCHHCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCEEEEEEHHHHCCHHCCCCHH
HRAVMDGKEQTGVTIMEMCDKMDAGDILNYESVDIGKTDTTGDVYKQIITVGPQLLIETM
HHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHH
DLLEKNQVTPLKQDENQVSYAPKLKKEDEYLDFSKYTNTEVFNRVRGLNPWPGAFTKFEG
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHCCCCCCCCCCCCCCC
KRLKIWETKVHNSSSFNSNSKPGEIIEINQQGPVVKCCQGSVILTKIQPSGKKAMTGEQF
CEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEECCCCEEEEEECCCCCCCCCHHHH
IRGYDIKSGIQLE
HHCCCCCCCCCCC
>Mature Secondary Structure
MRTIFMGTPDFSVPFIEAIARSTHNLNLVVTQPDRRKGRGKELQPPPAKRKAEELGIDVF
CCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCHHHCCCCCCCCCCCHHHHHHHCCCEEE
QPESIHNNYAYQILSDIEPHLIVTAAYGQILPRKILDLPRIKAINVHASLLPEYRGAAPI
CCCHHCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCEEEEEEHHHHCCHHCCCCHH
HRAVMDGKEQTGVTIMEMCDKMDAGDILNYESVDIGKTDTTGDVYKQIITVGPQLLIETM
HHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHH
DLLEKNQVTPLKQDENQVSYAPKLKKEDEYLDFSKYTNTEVFNRVRGLNPWPGAFTKFEG
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHCCCCCCCCCCCCCCC
KRLKIWETKVHNSSSFNSNSKPGEIIEINQQGPVVKCCQGSVILTKIQPSGKKAMTGEQF
CEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEECCCCEEEEEECCCCCCCCCHHHH
IRGYDIKSGIQLE
HHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA