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The map label for this gene is preA [H]

Identifier: 188585880

GI number: 188585880

Start: 1327507

End: 1328520

Strand: Reverse

Name: preA [H]

Synonym: Nther_1254

Alternate gene names: 188585880

Gene position: 1328520-1327507 (Counterclockwise)

Preceding gene: 188585881

Following gene: 188585876

Centisome position: 41.97

GC content: 36.69

Gene sequence:

>1014_bases
GTGAAATCTATCGATAACAACAATCCCCAAATCGCCAATTTTAAGTCTCTAATACTCCAAAACTTACAAGAAACATTGCT
TAACGATGGAGATACAGAAGTTCAATCTATCATTAATCATCTTTTATCAAGACAAGGAAAGCTTTTTCGACCCGTCCTTG
TATATTTATCAGCCAATATTCATCAAGTTGATAAAGATAGGGTATTGAAAACTGCCATGGCTTCTGAGTTGATTCACCTA
GCTTCTTTGGTCCACGATGATATCATAGATAATTCTGACTATCGACGGGGGAAACAAACTGTAAATTATTACTGGGGTAA
TAAATCAGCCGTGCTAGTTGGTGATTATTTATTTTCAAAGGCTTTTTCAATTCTTGCTATGATTGATTCTCCCAAAATTA
TGGCTCTATTTACCGATGCCATCAGTAAAATGTGTCAAGGAGAGCTTATTCAGTTAAATAACGCCTTTAAAAGTGGTATT
TCTAGAGAAATTTATTTTAAAGTGATTGAATATAAAACAGCTGCACTACTTGCAGCTTGTTGTGAGTCCGGTGCTATCTT
AAGTTCAGGGAATGGAAAGTCTTGTAAATCGGCAGCAGCTTTAAAAAATTATGGACTTCATTTGGGTAGAGCTTACCAAA
TCATCGATGATGTAATGGATTATACTGTACCCATGGGTTCTAGCGAAAAACCACGAGGGAAAGACCTTACTGAAGGCATT
ATTACTTTACCCGTAATTTTATTAATGGAAAAGAATGAGTTGACAGGGCAAGAGCTAGAAGAATTTTGGCAGGCAGGTCC
GACCCCAAACCAGTTTAGTCTCCTTGTGCGAGACCTTAATAATAACAGATGTATTACTGAAGCCCTCAGAATTGCTGAAC
ATGAGTTGAATTTAGCTAATCAATATTTAACACAAATTAGTGGAGAGTACAGTGAGCTCGATGTCTTGTTTTCTATGAAA
AGTCAACTTTTACAGCGTGTAAACAAACTACAATCAGTCACCGCCGAAATCTGA

Upstream 100 bases:

>100_bases
ATTGTCTGAGTAATATTAGAATTAACTCAGTTTAGATAAAATGACCAATTATTGCTAATACTAGGATTAAGTATAAATTT
TAAACTTGGAGTGATAAATT

Downstream 100 bases:

>100_bases
AATTGTAAATGGCTGTATTAAGTTGTATTTTGAAAATGTAAAGAGTATCAACAACTAAATATAACAGCGCCTAAGTAATA
ATAAATTAAATCTAGATTAC

Product: Polyprenyl synthetase

Products: trans-polyisoprenyln-PP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKSIDNNNPQIANFKSLILQNLQETLLNDGDTEVQSIINHLLSRQGKLFRPVLVYLSANIHQVDKDRVLKTAMASELIHL
ASLVHDDIIDNSDYRRGKQTVNYYWGNKSAVLVGDYLFSKAFSILAMIDSPKIMALFTDAISKMCQGELIQLNNAFKSGI
SREIYFKVIEYKTAALLAACCESGAILSSGNGKSCKSAAALKNYGLHLGRAYQIIDDVMDYTVPMGSSEKPRGKDLTEGI
ITLPVILLMEKNELTGQELEEFWQAGPTPNQFSLLVRDLNNNRCITEALRIAEHELNLANQYLTQISGEYSELDVLFSMK
SQLLQRVNKLQSVTAEI

Sequences:

>Translated_337_residues
MKSIDNNNPQIANFKSLILQNLQETLLNDGDTEVQSIINHLLSRQGKLFRPVLVYLSANIHQVDKDRVLKTAMASELIHL
ASLVHDDIIDNSDYRRGKQTVNYYWGNKSAVLVGDYLFSKAFSILAMIDSPKIMALFTDAISKMCQGELIQLNNAFKSGI
SREIYFKVIEYKTAALLAACCESGAILSSGNGKSCKSAAALKNYGLHLGRAYQIIDDVMDYTVPMGSSEKPRGKDLTEGI
ITLPVILLMEKNELTGQELEEFWQAGPTPNQFSLLVRDLNNNRCITEALRIAEHELNLANQYLTQISGEYSELDVLFSMK
SQLLQRVNKLQSVTAEI
>Mature_337_residues
MKSIDNNNPQIANFKSLILQNLQETLLNDGDTEVQSIINHLLSRQGKLFRPVLVYLSANIHQVDKDRVLKTAMASELIHL
ASLVHDDIIDNSDYRRGKQTVNYYWGNKSAVLVGDYLFSKAFSILAMIDSPKIMALFTDAISKMCQGELIQLNNAFKSGI
SREIYFKVIEYKTAALLAACCESGAILSSGNGKSCKSAAALKNYGLHLGRAYQIIDDVMDYTVPMGSSEKPRGKDLTEGI
ITLPVILLMEKNELTGQELEEFWQAGPTPNQFSLLVRDLNNNRCITEALRIAEHELNLANQYLTQISGEYSELDVLFSMK
SQLLQRVNKLQSVTAEI

Specific function: Possible role in synthesis of the nonaprenyl side chain of plastoquinone or in synthesis of other prenyl chains such as undekaprenyl pyrophosphate [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=227, Percent_Identity=35.2422907488987, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1789578, Length=210, Percent_Identity=36.6666666666667, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1786623, Length=226, Percent_Identity=30.9734513274336, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17505681, Length=201, Percent_Identity=37.3134328358209, Blast_Score=132, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6319475, Length=225, Percent_Identity=32, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6325188, Length=229, Percent_Identity=26.6375545851528, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24651612, Length=289, Percent_Identity=31.4878892733564, Blast_Score=135, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR014120
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: 2.5.1.- [C]

Molecular weight: Translated: 37645; Mature: 37645

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIDNNNPQIANFKSLILQNLQETLLNDGDTEVQSIINHLLSRQGKLFRPVLVYLSANI
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCH
HQVDKDRVLKTAMASELIHLASLVHDDIIDNSDYRRGKQTVNYYWGNKSAVLVGDYLFSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCEEEEEECCCCEEEEHHHHHHH
AFSILAMIDSPKIMALFTDAISKMCQGELIQLNNAFKSGISREIYFKVIEYKTAALLAAC
HHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
CESGAILSSGNGKSCKSAAALKNYGLHLGRAYQIIDDVMDYTVPMGSSEKPRGKDLTEGI
HCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
ITLPVILLMEKNELTGQELEEFWQAGPTPNQFSLLVRDLNNNRCITEALRIAEHELNLAN
HHHHHHHEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QYLTQISGEYSELDVLFSMKSQLLQRVNKLQSVTAEI
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSIDNNNPQIANFKSLILQNLQETLLNDGDTEVQSIINHLLSRQGKLFRPVLVYLSANI
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCH
HQVDKDRVLKTAMASELIHLASLVHDDIIDNSDYRRGKQTVNYYWGNKSAVLVGDYLFSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCEEEEEECCCCEEEEHHHHHHH
AFSILAMIDSPKIMALFTDAISKMCQGELIQLNNAFKSGISREIYFKVIEYKTAALLAAC
HHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
CESGAILSSGNGKSCKSAAALKNYGLHLGRAYQIIDDVMDYTVPMGSSEKPRGKDLTEGI
HCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
ITLPVILLMEKNELTGQELEEFWQAGPTPNQFSLLVRDLNNNRCITEALRIAEHELNLAN
HHHHHHHEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QYLTQISGEYSELDVLFSMKSQLLQRVNKLQSVTAEI
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: trans-polyisoprenyln-PP; Delta3-isopentenyl-PP [C]

Specific reaction: trans-polyisoprenyln-PP + Delta3-isopentenyl-PP = trans-polyisoprenyln-PP + pyrophosphate [C]

General reaction: Transferring alkyl or aryl groups, other than methyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]