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The map label for this gene is pmbA [C]

Identifier: 188585641

GI number: 188585641

Start: 1081364

End: 1082680

Strand: Reverse

Name: pmbA [C]

Synonym: Nther_1014

Alternate gene names: 188585641

Gene position: 1082680-1081364 (Counterclockwise)

Preceding gene: 188585642

Following gene: 188585633

Centisome position: 34.2

GC content: 35.61

Gene sequence:

>1317_bases
ATGGAAAATTTGTTAAATAAAGCCTTAAAAGTTGCCGATGAAGCTGAAGTTTACATGAGAGAAGTTTTTAATACTTCTGC
TTCAGTTAAAATGGGTGAAATGAAAGGAATAAATTCAGAGAAAAAAACAGAAGTAGCTTTGAGGATTATCAGAGATGGAA
ATATGGGAGCTGCAGTTTCCACATCTTTAGAGGATGATAGTATCATCGACCGAGCAGTTATAGCTTTAGAAAACCAAAAA
TCCGAGGCGGTATCCTTTCCTAATGAAAACTATCATGAAGTCCCTACATATTCTGAAGAAGTTAAAAATATGAGCAGTAA
AGAATTATCCCAACTTGCTTTTGATTATTACAATCGATTAAAAGAGGCCGGTCCAGATATTAAGTTTGATGTTAAGCTCC
AAAAATCATTTAAGAAAATTCATTTACTAAACAGTACTGGTTTCAATAACAGCTATGATTACACTAATTTTTCATTAGGT
TTAAACACACTAACAGATAAAGGCTTTACAGGCGCAAACAAAGAGTATTCCAGTGCAAAGCTACCTAAAGTTAGCAATGA
AAGTATTCAGAATCTAATCAAGCGACACCGTTTAGGTGATCACCCTGTTTCATTAGAAAACGAACAAATGCCAGTGATAT
TCAGTGGCAAAACCATGGGTGCTTTAATGTTGAGAGTCTTAGCTGGTGTTAATGGTGAAAATATAAATAAAGGGATTTCT
CCAGTAAAAGACAAACTAGGTGAACAGCTGTTTTCAGAAAACATCACGATTAGAGATGACGGTAAAATGCCCTACGGATG
TAATTCTTGTCCCTTTGACGATGAAGGGACCCCAGCACAAAATACCATCTTATATGATAAGGGAGTCCTGAAGAATTACT
TACTAGGTCTCAGTCAAACTAAAGAATTAGACTTAAAGCCAACAGGTAATGCATTTAAAAGGACCTTATTCTCTAAAGAA
ATTGAAGATAAACCTTCAGTTTTCGACTCAAACCTGGTAGTTGAAGGTAAGTCAAAATCAGATGCAGAGCTTATCAAGAA
TGTAGATAGAGGCCTGTTAATAACAGGCGTAATGGGAGCTCATACTGGTAATATAAATCAAGGTGACTTTTCTATGAACA
TTTCCTCAGGTTATCTGATTGAAAACGGTGAATTAGTGGGAGAAGTAAAAGGTAGCATGATTGCTGGTAATATTTATGAT
CTCTTTCAAAAAGTAGAAGCTGTCGGCACAGAATATGAAGCCATGAGAAGTATATTCTATTACATGGGCTATTCTCCCAT
GGTATTGTTCAGTGAAGCTAGTATTGTAGGAGAGTAA

Upstream 100 bases:

>100_bases
AAATCCGGTGGTTGCGGAAAAGCCGGTCAAATTTTAATCCAATCCGGAAAGGGTTCAGCCCCAATCAAAGTTAAAAATAT
GGGCATAGGGGGAAAATAAC

Downstream 100 bases:

>100_bases
TTTAATTATTAATATAAGAAAAAAGGGACTGTAACCCAAAATTATCATTTAGTTTGGGGACAGTCCCTTTTTGCTTTAAC
TGTTTCAACAATTTCAATTT

Product: peptidase U62 modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MENLLNKALKVADEAEVYMREVFNTSASVKMGEMKGINSEKKTEVALRIIRDGNMGAAVSTSLEDDSIIDRAVIALENQK
SEAVSFPNENYHEVPTYSEEVKNMSSKELSQLAFDYYNRLKEAGPDIKFDVKLQKSFKKIHLLNSTGFNNSYDYTNFSLG
LNTLTDKGFTGANKEYSSAKLPKVSNESIQNLIKRHRLGDHPVSLENEQMPVIFSGKTMGALMLRVLAGVNGENINKGIS
PVKDKLGEQLFSENITIRDDGKMPYGCNSCPFDDEGTPAQNTILYDKGVLKNYLLGLSQTKELDLKPTGNAFKRTLFSKE
IEDKPSVFDSNLVVEGKSKSDAELIKNVDRGLLITGVMGAHTGNINQGDFSMNISSGYLIENGELVGEVKGSMIAGNIYD
LFQKVEAVGTEYEAMRSIFYYMGYSPMVLFSEASIVGE

Sequences:

>Translated_438_residues
MENLLNKALKVADEAEVYMREVFNTSASVKMGEMKGINSEKKTEVALRIIRDGNMGAAVSTSLEDDSIIDRAVIALENQK
SEAVSFPNENYHEVPTYSEEVKNMSSKELSQLAFDYYNRLKEAGPDIKFDVKLQKSFKKIHLLNSTGFNNSYDYTNFSLG
LNTLTDKGFTGANKEYSSAKLPKVSNESIQNLIKRHRLGDHPVSLENEQMPVIFSGKTMGALMLRVLAGVNGENINKGIS
PVKDKLGEQLFSENITIRDDGKMPYGCNSCPFDDEGTPAQNTILYDKGVLKNYLLGLSQTKELDLKPTGNAFKRTLFSKE
IEDKPSVFDSNLVVEGKSKSDAELIKNVDRGLLITGVMGAHTGNINQGDFSMNISSGYLIENGELVGEVKGSMIAGNIYD
LFQKVEAVGTEYEAMRSIFYYMGYSPMVLFSEASIVGE
>Mature_438_residues
MENLLNKALKVADEAEVYMREVFNTSASVKMGEMKGINSEKKTEVALRIIRDGNMGAAVSTSLEDDSIIDRAVIALENQK
SEAVSFPNENYHEVPTYSEEVKNMSSKELSQLAFDYYNRLKEAGPDIKFDVKLQKSFKKIHLLNSTGFNNSYDYTNFSLG
LNTLTDKGFTGANKEYSSAKLPKVSNESIQNLIKRHRLGDHPVSLENEQMPVIFSGKTMGALMLRVLAGVNGENINKGIS
PVKDKLGEQLFSENITIRDDGKMPYGCNSCPFDDEGTPAQNTILYDKGVLKNYLLGLSQTKELDLKPTGNAFKRTLFSKE
IEDKPSVFDSNLVVEGKSKSDAELIKNVDRGLLITGVMGAHTGNINQGDFSMNISSGYLIENGELVGEVKGSMIAGNIYD
LFQKVEAVGTEYEAMRSIFYYMGYSPMVLFSEASIVGE

Specific function: May Facilitate The Secretion Of The Peptide Antibiotic Microcin B17 (Mccb17) By Completing Its Maturation. Suppresses The Inhibitory Activity Of The Carbon Storage Regulator (Csra). [C]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790682, Length=473, Percent_Identity=22.8329809725159, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789640, Length=203, Percent_Identity=29.5566502463054, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48432; Mature: 48432

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLLNKALKVADEAEVYMREVFNTSASVKMGEMKGINSEKKTEVALRIIRDGNMGAAVS
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCHHHHEEEEEEEECCCCCCEEE
TSLEDDSIIDRAVIALENQKSEAVSFPNENYHEVPTYSEEVKNMSSKELSQLAFDYYNRL
CCCCCHHHHHHHHHEECCCCHHHCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
KEAGPDIKFDVKLQKSFKKIHLLNSTGFNNSYDYTNFSLGLNTLTDKGFTGANKEYSSAK
HHCCCCCEEEEEHHHCCCCEEEEECCCCCCCCCCCEEEECCHHHCCCCCCCCCCCCCCCC
LPKVSNESIQNLIKRHRLGDHPVSLENEQMPVIFSGKTMGALMLRVLAGVNGENINKGIS
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCH
PVKDKLGEQLFSENITIRDDGKMPYGCNSCPFDDEGTPAQNTILYDKGVLKNYLLGLSQT
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCC
KELDLKPTGNAFKRTLFSKEIEDKPSVFDSNLVVEGKSKSDAELIKNVDRGLLITGVMGA
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCEEEEEECCC
HTGNINQGDFSMNISSGYLIENGELVGEVKGSMIAGNIYDLFQKVEAVGTEYEAMRSIFY
CCCCCCCCCEEEECCCCEEEECCCEEEECCCCEEECHHHHHHHHHHHHCCHHHHHHHHHH
YMGYSPMVLFSEASIVGE
HHCCCCEEEEECCHHCCC
>Mature Secondary Structure
MENLLNKALKVADEAEVYMREVFNTSASVKMGEMKGINSEKKTEVALRIIRDGNMGAAVS
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCHHHHEEEEEEEECCCCCCEEE
TSLEDDSIIDRAVIALENQKSEAVSFPNENYHEVPTYSEEVKNMSSKELSQLAFDYYNRL
CCCCCHHHHHHHHHEECCCCHHHCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
KEAGPDIKFDVKLQKSFKKIHLLNSTGFNNSYDYTNFSLGLNTLTDKGFTGANKEYSSAK
HHCCCCCEEEEEHHHCCCCEEEEECCCCCCCCCCCEEEECCHHHCCCCCCCCCCCCCCCC
LPKVSNESIQNLIKRHRLGDHPVSLENEQMPVIFSGKTMGALMLRVLAGVNGENINKGIS
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCH
PVKDKLGEQLFSENITIRDDGKMPYGCNSCPFDDEGTPAQNTILYDKGVLKNYLLGLSQT
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCC
KELDLKPTGNAFKRTLFSKEIEDKPSVFDSNLVVEGKSKSDAELIKNVDRGLLITGVMGA
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCEEEEEECCC
HTGNINQGDFSMNISSGYLIENGELVGEVKGSMIAGNIYDLFQKVEAVGTEYEAMRSIFY
CCCCCCCCCEEEECCCCEEEECCCEEEECCCCEEECHHHHHHHHHHHHCCHHHHHHHHHH
YMGYSPMVLFSEASIVGE
HHCCCCEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]