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The map label for this gene is glgB [H]

Identifier: 188585201

GI number: 188585201

Start: 589798

End: 591714

Strand: Reverse

Name: glgB [H]

Synonym: Nther_0562

Alternate gene names: 188585201

Gene position: 591714-589798 (Counterclockwise)

Preceding gene: 188585202

Following gene: 188585200

Centisome position: 18.69

GC content: 38.08

Gene sequence:

>1917_bases
TTGGTTCAAACCAAGACAATTACTGAATTTGAAACTCATCTTTTTCATGAAGGCACATATTTCGAAAGCTATCGTAAATT
TGGAGCTCACTTAAGTAATGAGAAAGGAACTGTTGGTACTCGATTTACTGTCTGGGCACCTAATGCCAAGAAAATTGAAA
TTGTAGGCAGTTTCAACGACTGGCATGGTGAACAATACGCAATGAATAAAATCACAACTTCGGGAATTTGGTCATTATTT
ATACCAGAAGTTACTGAAGGAGAATTATATAAGTATAAAATTTATCCTGAGGGGGGAGGAGAACCCCTATTGAAAGCTGA
TCCCTATGCTTTCTATTCTGAAAAAAGACCAAAATCAGCATCCATCGTTTATTCTCTAGAGGGATATAACTGGGGAGATA
GGGAATGGCAACGAAAAAAGAGACGAGAGAATATTTTAAAAAAACCTGTATCCATTTACGAAGTACACTTAGGTAGCTGG
AAAAAAACTGAAGATGGAGATTTTTTATCTTATCGTGAATTAGCCCACGATTTAGTCGATTATGTAAAAGAAATGGGCTA
CACTCATATAGAGCTCCTCCCAGTTGTTGAACATCCTTTTGACCGTTCGTGGGGTTATCAGGCCACTGGGTATTTTTCTG
TCACCAGCCGTTATGGTACCCCGAAGGATTTTATGTATTTTGTAGATCGGTGTCATCAAGAAGGTATCGGGGTAATTCTG
GATTGGAACCCCTGTCATTTCTGTAGAGATGGTCACGGTTTGAGACTCTTTGATGGAGCACCGGTTTTTGAATATCAAGA
TCCCCACAGGGCTGAAAATACTGAATGGGATACTAATAACTTCGATCTTGGGAAACCAGAAGTTATCAGTTTCTTAGTTT
CTAATGCTTTTTTCTGGTTTGACCTTTTTCATATTGATGGTCTTAGAGTAGACGCTGTCTCAAGTATTTTGTATATGGAT
TACGGTAAAGAAGATGGTGAAGAATGGTTACCGAATAAATACGGAGGCAGAGAAAATTTAGAAGGATTAAATTTTCTCAG
GACATTAAACGAAGCTGTTTTTGAACGGTTCCCTAATGTAATGATGGTTGCAGAAGAATCTACTTCTTGGCCCTTAGTCA
GTAAACCTACATATTCAGGTGGGCTAGGTTTTAACTTCAAATGGAATATGGGTTGGATGAATGACATGTTAGAGTACATG
AAAAAAGACCCAATACACCGAAAACATCACCACCAAAATATCACATTTTCTTTCTTATACGCTTTTTCCGAAAATTTCGT
TTTGCCATTGTCCCATGACGAGGTAGTTCATAGTAAAAGGTCTCTTCTCAATAAAATGCCCGGTGATTATTGGCAAAAGT
TTGCCAATTTAAGAGTATTACTAGGATATCAAATGGGTCACCCAGGAAAAAAACTATTATTCATGGGTGGAGAACTGGGG
CAATTTGATGAATGGAAAGACTTAGAGCAATTGGACTGGAACCTATTACAATTTGAATTTCATTGGAAGATGAGGCAGTA
CTGTCGTCAGTTAAATCAACTTTACACAACAGAACCAGCTCTCTACGAACAAGATCATACATCTAAAGGTTTTGAGTGGA
TAGATCCTCATGATCACAGTCAAAGTATTATCAGTTTTATTAGATGGGGGTATAATTTCCAGGATCATTTAATTATAGTA
TGTAATTTTACGCCTCAAGTATATCACGGTTACAGGATTGGAGTTCCCAAAGATGTGAGTTATAAAGAAATATTTAACAG
TGACGCTGAGGAGTATGGTGGTTCCGGCATCTTAAACTCAGATGAACTGCAACCCCATGAGGTTGAATGGCATCATCGCC
CGTATTCCATTGAACTTAATATCCCTCCCCTGGCCACCTTTATTTTAAAACCTTTGGCAGCTAGAGAGGAGAATTAG

Upstream 100 bases:

>100_bases
TATTTAAATACAGCTGATAAATTTCACATAAATACCATGTTTATGGGAAGAATTAGTATTTGAAAGTTTATTTACAATCT
TTACAAAGGGGAGTATGACT

Downstream 100 bases:

>100_bases
AATGATTAATAATGGTCAAGATTGGGTCGCCATGATTTTAGCTGGTGGTAAAGGGTCGAGATTAAATGAACTCACTTCCG
AGACGGCTAAACCCGCCGTC

Product: 1,4-alpha-glucan branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE [H]

Number of amino acids: Translated: 638; Mature: 638

Protein sequence:

>638_residues
MVQTKTITEFETHLFHEGTYFESYRKFGAHLSNEKGTVGTRFTVWAPNAKKIEIVGSFNDWHGEQYAMNKITTSGIWSLF
IPEVTEGELYKYKIYPEGGGEPLLKADPYAFYSEKRPKSASIVYSLEGYNWGDREWQRKKRRENILKKPVSIYEVHLGSW
KKTEDGDFLSYRELAHDLVDYVKEMGYTHIELLPVVEHPFDRSWGYQATGYFSVTSRYGTPKDFMYFVDRCHQEGIGVIL
DWNPCHFCRDGHGLRLFDGAPVFEYQDPHRAENTEWDTNNFDLGKPEVISFLVSNAFFWFDLFHIDGLRVDAVSSILYMD
YGKEDGEEWLPNKYGGRENLEGLNFLRTLNEAVFERFPNVMMVAEESTSWPLVSKPTYSGGLGFNFKWNMGWMNDMLEYM
KKDPIHRKHHHQNITFSFLYAFSENFVLPLSHDEVVHSKRSLLNKMPGDYWQKFANLRVLLGYQMGHPGKKLLFMGGELG
QFDEWKDLEQLDWNLLQFEFHWKMRQYCRQLNQLYTTEPALYEQDHTSKGFEWIDPHDHSQSIISFIRWGYNFQDHLIIV
CNFTPQVYHGYRIGVPKDVSYKEIFNSDAEEYGGSGILNSDELQPHEVEWHHRPYSIELNIPPLATFILKPLAAREEN

Sequences:

>Translated_638_residues
MVQTKTITEFETHLFHEGTYFESYRKFGAHLSNEKGTVGTRFTVWAPNAKKIEIVGSFNDWHGEQYAMNKITTSGIWSLF
IPEVTEGELYKYKIYPEGGGEPLLKADPYAFYSEKRPKSASIVYSLEGYNWGDREWQRKKRRENILKKPVSIYEVHLGSW
KKTEDGDFLSYRELAHDLVDYVKEMGYTHIELLPVVEHPFDRSWGYQATGYFSVTSRYGTPKDFMYFVDRCHQEGIGVIL
DWNPCHFCRDGHGLRLFDGAPVFEYQDPHRAENTEWDTNNFDLGKPEVISFLVSNAFFWFDLFHIDGLRVDAVSSILYMD
YGKEDGEEWLPNKYGGRENLEGLNFLRTLNEAVFERFPNVMMVAEESTSWPLVSKPTYSGGLGFNFKWNMGWMNDMLEYM
KKDPIHRKHHHQNITFSFLYAFSENFVLPLSHDEVVHSKRSLLNKMPGDYWQKFANLRVLLGYQMGHPGKKLLFMGGELG
QFDEWKDLEQLDWNLLQFEFHWKMRQYCRQLNQLYTTEPALYEQDHTSKGFEWIDPHDHSQSIISFIRWGYNFQDHLIIV
CNFTPQVYHGYRIGVPKDVSYKEIFNSDAEEYGGSGILNSDELQPHEVEWHHRPYSIELNIPPLATFILKPLAAREEN
>Mature_638_residues
MVQTKTITEFETHLFHEGTYFESYRKFGAHLSNEKGTVGTRFTVWAPNAKKIEIVGSFNDWHGEQYAMNKITTSGIWSLF
IPEVTEGELYKYKIYPEGGGEPLLKADPYAFYSEKRPKSASIVYSLEGYNWGDREWQRKKRRENILKKPVSIYEVHLGSW
KKTEDGDFLSYRELAHDLVDYVKEMGYTHIELLPVVEHPFDRSWGYQATGYFSVTSRYGTPKDFMYFVDRCHQEGIGVIL
DWNPCHFCRDGHGLRLFDGAPVFEYQDPHRAENTEWDTNNFDLGKPEVISFLVSNAFFWFDLFHIDGLRVDAVSSILYMD
YGKEDGEEWLPNKYGGRENLEGLNFLRTLNEAVFERFPNVMMVAEESTSWPLVSKPTYSGGLGFNFKWNMGWMNDMLEYM
KKDPIHRKHHHQNITFSFLYAFSENFVLPLSHDEVVHSKRSLLNKMPGDYWQKFANLRVLLGYQMGHPGKKLLFMGGELG
QFDEWKDLEQLDWNLLQFEFHWKMRQYCRQLNQLYTTEPALYEQDHTSKGFEWIDPHDHSQSIISFIRWGYNFQDHLIIV
CNFTPQVYHGYRIGVPKDVSYKEIFNSDAEEYGGSGILNSDELQPHEVEWHHRPYSIELNIPPLATFILKPLAAREEN

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position [H]

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=663, Percent_Identity=26.9984917043741, Blast_Score=194, Evalue=2e-49,
Organism=Escherichia coli, GI1789839, Length=627, Percent_Identity=47.0494417862839, Blast_Score=585, Evalue=1e-168,
Organism=Caenorhabditis elegans, GI17554896, Length=653, Percent_Identity=26.7993874425727, Blast_Score=191, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI32564391, Length=598, Percent_Identity=26.7558528428094, Blast_Score=168, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6320826, Length=680, Percent_Identity=26.4705882352941, Blast_Score=184, Evalue=4e-47,
Organism=Drosophila melanogaster, GI28573410, Length=655, Percent_Identity=26.1068702290076, Blast_Score=204, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48 [H]

EC number: =2.4.1.18 [H]

Molecular weight: Translated: 74974; Mature: 74974

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQTKTITEFETHLFHEGTYFESYRKFGAHLSNEKGTVGTRFTVWAPNAKKIEIVGSFND
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEEEEECCCC
WHGEQYAMNKITTSGIWSLFIPEVTEGELYKYKIYPEGGGEPLLKADPYAFYSEKRPKSA
CCCCHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHCCCCCCCC
SIVYSLEGYNWGDREWQRKKRRENILKKPVSIYEVHLGSWKKTEDGDFLSYRELAHDLVD
EEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
YVKEMGYTHIELLPVVEHPFDRSWGYQATGYFSVTSRYGTPKDFMYFVDRCHQEGIGVIL
HHHHCCCCEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEE
DWNPCHFCRDGHGLRLFDGAPVFEYQDPHRAENTEWDTNNFDLGKPEVISFLVSNAFFWF
ECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEE
DLFHIDGLRVDAVSSILYMDYGKEDGEEWLPNKYGGRENLEGLNFLRTLNEAVFERFPNV
EEEEECCCCHHHHHHHHEEECCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCE
MMVAEESTSWPLVSKPTYSGGLGFNFKWNMGWMNDMLEYMKKDPIHRKHHHQNITFSFLY
EEEEECCCCCCEECCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCHHHCCCCCEEEEEE
AFSENFVLPLSHDEVVHSKRSLLNKMPGDYWQKFANLRVLLGYQMGHPGKKLLFMGGELG
EECCCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHCEEEEEEEECCCCCCEEEEECCCCC
QFDEWKDLEQLDWNLLQFEFHWKMRQYCRQLNQLYTTEPALYEQDHTSKGFEWIDPHDHS
CCCCCCCHHHHCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCH
QSIISFIRWGYNFQDHLIIVCNFTPQVYHGYRIGVPKDVSYKEIFNSDAEEYGGSGILNS
HHHHHHHHHCCCCCCCEEEEECCCHHHHCCEECCCCCCCCHHHHHCCCHHHHCCCCCCCC
DELQPHEVEWHHRPYSIELNIPPLATFILKPLAAREEN
CCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVQTKTITEFETHLFHEGTYFESYRKFGAHLSNEKGTVGTRFTVWAPNAKKIEIVGSFND
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEEEEECCCC
WHGEQYAMNKITTSGIWSLFIPEVTEGELYKYKIYPEGGGEPLLKADPYAFYSEKRPKSA
CCCCHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHCCCCCCCC
SIVYSLEGYNWGDREWQRKKRRENILKKPVSIYEVHLGSWKKTEDGDFLSYRELAHDLVD
EEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
YVKEMGYTHIELLPVVEHPFDRSWGYQATGYFSVTSRYGTPKDFMYFVDRCHQEGIGVIL
HHHHCCCCEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEE
DWNPCHFCRDGHGLRLFDGAPVFEYQDPHRAENTEWDTNNFDLGKPEVISFLVSNAFFWF
ECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEE
DLFHIDGLRVDAVSSILYMDYGKEDGEEWLPNKYGGRENLEGLNFLRTLNEAVFERFPNV
EEEEECCCCHHHHHHHHEEECCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCE
MMVAEESTSWPLVSKPTYSGGLGFNFKWNMGWMNDMLEYMKKDPIHRKHHHQNITFSFLY
EEEEECCCCCCEECCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCHHHCCCCCEEEEEE
AFSENFVLPLSHDEVVHSKRSLLNKMPGDYWQKFANLRVLLGYQMGHPGKKLLFMGGELG
EECCCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHCEEEEEEEECCCCCCEEEEECCCCC
QFDEWKDLEQLDWNLLQFEFHWKMRQYCRQLNQLYTTEPALYEQDHTSKGFEWIDPHDHS
CCCCCCCHHHHCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCH
QSIISFIRWGYNFQDHLIIVCNFTPQVYHGYRIGVPKDVSYKEIFNSDAEEYGGSGILNS
HHHHHHHHHCCCCCCCEEEEECCCHHHHCCEECCCCCCCCHHHHHCCCHHHHCCCCCCCC
DELQPHEVEWHHRPYSIELNIPPLATFILKPLAAREEN
CCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA