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The map label for this gene is purU [H]

Identifier: 188528197

GI number: 188528197

Start: 1425011

End: 1425892

Strand: Reverse

Name: purU [H]

Synonym: HPSH_07305

Alternate gene names: 188528197

Gene position: 1425892-1425011 (Counterclockwise)

Preceding gene: 188528198

Following gene: 188528196

Centisome position: 88.64

GC content: 39.0

Gene sequence:

>882_bases
ATGTTAGAATTTATTTTAAAAATTCAAGCTAGAGACTCTAAAGGCTTGGTGAGCGCGATTAGCACCACTATTGCTAACAA
GGGCTATAATATTGTCAAAAACGATGAATTTGTTGATCCCTTAAAACAACGCTTTTTCATGCGGCTAAAGATCCAAAAAG
AAATTAAGCCCTTGAATACTGGAATTAAAGAGCAAGAAGAGCAATCCTTAAAAACCGCTCTTTTTAAAGCCCTAGAAAAC
TTTAACGAACTATTGATTGAAGTCATTTTAACGCATAAAAAAAACATCATTCTGCTCGCTACTAAAGAGAGCCATTGCTT
AGGGGATTTGCTTTTAAGGGTGTATGGAGGGGAATTGAACGCTCAAATTTTGGGCGTTATTTCCAATCACGAGATTTTAC
GCCCTTTAGTGGAAAAATTTGACATCCCTTATTTTTATGCGCCTTGCGATAATCAAGTTTTGCATGAAAAAGAAGTTTTA
GCAATCATTAAAAACCTGGAATTAAAACACAAAGTGAGCGCGGATTTGCTCGTTTTAGCCAAATACATGCGCATTTTAAG
CCATGATTTTACGAAGCGCTATGAAAACCAGATTCTCAATATCCATCATAGTTTCTTGCCCGCATTCATCGGGGCTAACC
CTTACCAGCAAGCGTTTGAAAGGGGCGTGAAAGTCATCGGGGCCACGGTGCATTTTGTGAATGAAAGCCTTGATGCCGGG
CCGATTATCATACAAGACACTCTGCCCATTAACCACAATTACAGCGTGGAAAAAATGCGCCTAGCGGGTAAGGATATAGA
AAAACTGGTTTTGGCTAGGGCTTTAAAACTGGTTTTAGAAGATCGGGTGTTTGTGCATGAAAATAAAACGGTGGTGTTTT
GA

Upstream 100 bases:

>100_bases
TAGAAAAAAGCCCCATGGAGCGCTTCATTGAAAAAGCCACGCAATCAGCGACAAATATCATCACGCAAGCGTTTGGCTAT
CAATTATTAATGAGATGAAC

Downstream 100 bases:

>100_bases
ATGCTTCCATTAATGCTGCCCTTAAATTGGCAAAGAGTGAGGCTTGGGGATATAGGCAAACCATGCATGTGTAAAAGAGT
GATGAAGCATCAAACAACAC

Product: formyltetrahydrofolate hydrolase (purU)

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MLEFILKIQARDSKGLVSAISTTIANKGYNIVKNDEFVDPLKQRFFMRLKIQKEIKPLNTGIKEQEEQSLKTALFKALEN
FNELLIEVILTHKKNIILLATKESHCLGDLLLRVYGGELNAQILGVISNHEILRPLVEKFDIPYFYAPCDNQVLHEKEVL
AIIKNLELKHKVSADLLVLAKYMRILSHDFTKRYENQILNIHHSFLPAFIGANPYQQAFERGVKVIGATVHFVNESLDAG
PIIIQDTLPINHNYSVEKMRLAGKDIEKLVLARALKLVLEDRVFVHENKTVVF

Sequences:

>Translated_293_residues
MLEFILKIQARDSKGLVSAISTTIANKGYNIVKNDEFVDPLKQRFFMRLKIQKEIKPLNTGIKEQEEQSLKTALFKALEN
FNELLIEVILTHKKNIILLATKESHCLGDLLLRVYGGELNAQILGVISNHEILRPLVEKFDIPYFYAPCDNQVLHEKEVL
AIIKNLELKHKVSADLLVLAKYMRILSHDFTKRYENQILNIHHSFLPAFIGANPYQQAFERGVKVIGATVHFVNESLDAG
PIIIQDTLPINHNYSVEKMRLAGKDIEKLVLARALKLVLEDRVFVHENKTVVF
>Mature_293_residues
MLEFILKIQARDSKGLVSAISTTIANKGYNIVKNDEFVDPLKQRFFMRLKIQKEIKPLNTGIKEQEEQSLKTALFKALEN
FNELLIEVILTHKKNIILLATKESHCLGDLLLRVYGGELNAQILGVISNHEILRPLVEKFDIPYFYAPCDNQVLHEKEVL
AIIKNLELKHKVSADLLVLAKYMRILSHDFTKRYENQILNIHHSFLPAFIGANPYQQAFERGVKVIGATVHFVNESLDAG
PIIIQDTLPINHNYSVEKMRLAGKDIEKLVLARALKLVLEDRVFVHENKTVVF

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=121, Percent_Identity=41.3223140495868, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI209869995, Length=121, Percent_Identity=41.3223140495868, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI209869993, Length=121, Percent_Identity=41.3223140495868, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1787483, Length=289, Percent_Identity=44.2906574394464, Blast_Score=238, Evalue=4e-64,
Organism=Escherichia coli, GI1788846, Length=125, Percent_Identity=35.2, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17567511, Length=144, Percent_Identity=34.0277777777778, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24582400, Length=111, Percent_Identity=35.1351351351351, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 33518; Mature: 33518

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEFILKIQARDSKGLVSAISTTIANKGYNIVKNDEFVDPLKQRFFMRLKIQKEIKPLNT
CCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GIKEQEEQSLKTALFKALENFNELLIEVILTHKKNIILLATKESHCLGDLLLRVYGGELN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCC
AQILGVISNHEILRPLVEKFDIPYFYAPCDNQVLHEKEVLAIIKNLELKHKVSADLLVLA
EEEEEEECCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KYMRILSHDFTKRYENQILNIHHSFLPAFIGANPYQQAFERGVKVIGATVHFVNESLDAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
PIIIQDTLPINHNYSVEKMRLAGKDIEKLVLARALKLVLEDRVFVHENKTVVF
CEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEECCCEEEC
>Mature Secondary Structure
MLEFILKIQARDSKGLVSAISTTIANKGYNIVKNDEFVDPLKQRFFMRLKIQKEIKPLNT
CCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GIKEQEEQSLKTALFKALENFNELLIEVILTHKKNIILLATKESHCLGDLLLRVYGGELN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCC
AQILGVISNHEILRPLVEKFDIPYFYAPCDNQVLHEKEVLAIIKNLELKHKVSADLLVLA
EEEEEEECCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KYMRILSHDFTKRYENQILNIHHSFLPAFIGANPYQQAFERGVKVIGATVHFVNESLDAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
PIIIQDTLPINHNYSVEKMRLAGKDIEKLVLARALKLVLEDRVFVHENKTVVF
CEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 8335255 [H]