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The map label for this gene is thiD [H]

Identifier: 188527309

GI number: 188527309

Start: 501048

End: 501857

Strand: Direct

Name: thiD [H]

Synonym: HPSH_02580

Alternate gene names: 188527309

Gene position: 501048-501857 (Clockwise)

Preceding gene: 188527308

Following gene: 188527310

Centisome position: 31.15

GC content: 44.07

Gene sequence:

>810_bases
GTGAAAATTTACCCGCAAGTTTTAAGCATTGCCGGCAGCGATAGCGGTGGGGGTGCTGGGATACAAGCCGATTTGAAAGC
GTTCCAAACTTTGGGCGTGTTTGGGACAAGCGTGATCACTTGCATCACCGCACAAAACACACAGGGCGTGCATGGGGTTT
ATCCGTTGGGCGTTGAGAGCGTGAGGGCGCAAATCTTAGCCATTAGAGATGATTTTTCTATCAAAGCGTTCAAAATGGGA
GCGTTATGCAACGCTCAAATCATTGAATGCGTGGCGGACACTTTAAAAACATGCGATTTTGGGTTGTGCGTTTTAGATCC
GGTGATGGTGGCAAAGAATGGGGCTTTGCTTTTAGAAGAAGAGGCGATTTTAAGCTTAAAAAAACGCCTTTTACCTAAAA
CCCATTTACTAACCCCTAACCTCCCTGAAGTTTATGCGCTCACAGGCGTTCAAGTGCGAGATAATAAAAGCGCTTCAAAA
GCGATGGGTATTTTAAGGGATTTAGGCGTTAAAAACGCTGTGATTAAAGGGGGGCATACAGAGCATTTTCAAGGGGAATT
TAGCAACGACTGGGTGTTTTTAGAAGACGCTGAATTTATCCTAAACGCCAAGCGATTCAACACGAAAAACACGCATGGCA
CGGGTTGTGTTCTGTCTAGCTTGATAGTGGGCTTACTCGCTCAAGGGTTGGATTTGAAAAACGCTATTTCAAAGGCTAAA
GAGCTTTTAACTATCATCATTCAAAACCCTTTAAACATTGGGCATGGGCATGGGCCTTTGAATTTATGGAGTATTAAAAA
GCTTGTTTGA

Upstream 100 bases:

>100_bases
TGTCAGAAAATAACGGCTCTAATGGTTCGTTCTTGTTTTATTTTTTAGACGCTCTAAGCTTGCCCATAAAGCTAGAAAAT
AGCCTTATTAAGGAAGAGTT

Downstream 100 bases:

>100_bases
TGCGAATTGTTTGAAACTCATGTTTGTGGCTGGTTCGCAAGACTTCTACCACATAAAGGGCGGCAAAAACGATAGGATAA
ACGCGCTTTTAGACGCTTTA

Product: thiamine biosynthesis protein (thi)

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MKIYPQVLSIAGSDSGGGAGIQADLKAFQTLGVFGTSVITCITAQNTQGVHGVYPLGVESVRAQILAIRDDFSIKAFKMG
ALCNAQIIECVADTLKTCDFGLCVLDPVMVAKNGALLLEEEAILSLKKRLLPKTHLLTPNLPEVYALTGVQVRDNKSASK
AMGILRDLGVKNAVIKGGHTEHFQGEFSNDWVFLEDAEFILNAKRFNTKNTHGTGCVLSSLIVGLLAQGLDLKNAISKAK
ELLTIIIQNPLNIGHGHGPLNLWSIKKLV

Sequences:

>Translated_269_residues
MKIYPQVLSIAGSDSGGGAGIQADLKAFQTLGVFGTSVITCITAQNTQGVHGVYPLGVESVRAQILAIRDDFSIKAFKMG
ALCNAQIIECVADTLKTCDFGLCVLDPVMVAKNGALLLEEEAILSLKKRLLPKTHLLTPNLPEVYALTGVQVRDNKSASK
AMGILRDLGVKNAVIKGGHTEHFQGEFSNDWVFLEDAEFILNAKRFNTKNTHGTGCVLSSLIVGLLAQGLDLKNAISKAK
ELLTIIIQNPLNIGHGHGPLNLWSIKKLV
>Mature_269_residues
MKIYPQVLSIAGSDSGGGAGIQADLKAFQTLGVFGTSVITCITAQNTQGVHGVYPLGVESVRAQILAIRDDFSIKAFKMG
ALCNAQIIECVADTLKTCDFGLCVLDPVMVAKNGALLLEEEAILSLKKRLLPKTHLLTPNLPEVYALTGVQVRDNKSASK
AMGILRDLGVKNAVIKGGHTEHFQGEFSNDWVFLEDAEFILNAKRFNTKNTHGTGCVLSSLIVGLLAQGLDLKNAISKAK
ELLTIIIQNPLNIGHGHGPLNLWSIKKLV

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=257, Percent_Identity=40.8560311284047, Blast_Score=185, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6325378, Length=263, Percent_Identity=30.0380228136882, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324517, Length=275, Percent_Identity=31.6363636363636, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324997, Length=278, Percent_Identity=33.0935251798561, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28828; Mature: 28828

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYPQVLSIAGSDSGGGAGIQADLKAFQTLGVFGTSVITCITAQNTQGVHGVYPLGVES
CCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCHHH
VRAQILAIRDDFSIKAFKMGALCNAQIIECVADTLKTCDFGLCVLDPVMVAKNGALLLEE
HHHEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEH
EAILSLKKRLLPKTHLLTPNLPEVYALTGVQVRDNKSASKAMGILRDLGVKNAVIKGGHT
HHHHHHHHHHCCCHHCCCCCCCCEEEEECEEEECCCCHHHHHHHHHHCCCCHHEECCCCC
EHFQGEFSNDWVFLEDAEFILNAKRFNTKNTHGTGCVLSSLIVGLLAQGLDLKNAISKAK
CCCCCCCCCCEEEEECHHHHEEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
ELLTIIIQNPLNIGHGHGPLNLWSIKKLV
HHHHHHHHCCCCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MKIYPQVLSIAGSDSGGGAGIQADLKAFQTLGVFGTSVITCITAQNTQGVHGVYPLGVES
CCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCHHH
VRAQILAIRDDFSIKAFKMGALCNAQIIECVADTLKTCDFGLCVLDPVMVAKNGALLLEE
HHHEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEH
EAILSLKKRLLPKTHLLTPNLPEVYALTGVQVRDNKSASKAMGILRDLGVKNAVIKGGHT
HHHHHHHHHHCCCHHCCCCCCCCEEEEECEEEECCCCHHHHHHHHHHCCCCHHEECCCCC
EHFQGEFSNDWVFLEDAEFILNAKRFNTKNTHGTGCVLSSLIVGLLAQGLDLKNAISKAK
CCCCCCCCCCEEEEECHHHHEEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
ELLTIIIQNPLNIGHGHGPLNLWSIKKLV
HHHHHHHHCCCCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9252185 [H]