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The map label for this gene is thiM [H]

Identifier: 188527308

GI number: 188527308

Start: 500272

End: 501051

Strand: Direct

Name: thiM [H]

Synonym: HPSH_02575

Alternate gene names: 188527308

Gene position: 500272-501051 (Clockwise)

Preceding gene: 188527307

Following gene: 188527309

Centisome position: 31.1

GC content: 42.82

Gene sequence:

>780_bases
ATGTTAAAAGAGTTACGCCAAAAACGCCCCTTAGTGCATAATATCACCAATTATGTGGTGGCGCAATTCGTGGCTAATGG
TTTATTAGCCTTAGGGGCATCGCCTTTAATGAGTGATGCCATTGCTGAAATGCAAGATTTAGCAAAAATTTCTGACGCGC
TCGCTATCAATATTGGCACTCTCAATGAACGCACCATTTTATGCGCTAAAGAGGCTATCAAACATTATAAGGCTTTGAAT
AAACCCATTGTGTTAGATCCTGTGGGGTGTTCAGCGAGCGCTTTACGCCATGGCACAAGTTTAGAGCTTTTAGAAAGCGA
AGGGATTAGCGTTCTTAGGGGTAATGCTGCAGAATTAGGCTCTTTAGTGGGGATTTCGTGCGGAAGTAAGGGGCTAGACT
CTCATTATGCCACCACGCCTATAGAAATAGTCAAACTAGTGGCTCAAAAATATTCTGTGATAGCGGTAATGACGGGTAAA
ACAGATTATGTGAGCGATGGGAAAAAAGTTTTTAGCATTACTGGGGGGAGCGAGTATTTAGCGCTCATTACTGGGGCTGG
GTGTTTGCACGCAGCAGCGTGCGCGAGCTTTTTAAGTTTGAGAAAAGACCCCCTAGATTCTATGGCGCAACTTTGCGCGC
TTTATAAACAAGCCGCTTTTAATGCGCAAAAAAAGGTGTCAGAAAATAACGGCTCTAATGGTTCGTTCTTGTTTTATTTT
TTAGACGCTCTAAGCTTGCCCATAAAGCTAGAAAATAGCCTTATTAAGGAAGAGTTGTGA

Upstream 100 bases:

>100_bases
TTGATGAAAAATAGTTCATTTTTGAACGCTTTTGTATTAAAAGACAGCACCGCTTAATGGATTTTTTTGTAAAATAAAAG
AAAGTTTAAGGAGGTTCATA

Downstream 100 bases:

>100_bases
AAATTTACCCGCAAGTTTTAAGCATTGCCGGCAGCGATAGCGGTGGGGGTGCTGGGATACAAGCCGATTTGAAAGCGTTC
CAAACTTTGGGCGTGTTTGG

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLKELRQKRPLVHNITNYVVAQFVANGLLALGASPLMSDAIAEMQDLAKISDALAINIGTLNERTILCAKEAIKHYKALN
KPIVLDPVGCSASALRHGTSLELLESEGISVLRGNAAELGSLVGISCGSKGLDSHYATTPIEIVKLVAQKYSVIAVMTGK
TDYVSDGKKVFSITGGSEYLALITGAGCLHAAACASFLSLRKDPLDSMAQLCALYKQAAFNAQKKVSENNGSNGSFLFYF
LDALSLPIKLENSLIKEEL

Sequences:

>Translated_259_residues
MLKELRQKRPLVHNITNYVVAQFVANGLLALGASPLMSDAIAEMQDLAKISDALAINIGTLNERTILCAKEAIKHYKALN
KPIVLDPVGCSASALRHGTSLELLESEGISVLRGNAAELGSLVGISCGSKGLDSHYATTPIEIVKLVAQKYSVIAVMTGK
TDYVSDGKKVFSITGGSEYLALITGAGCLHAAACASFLSLRKDPLDSMAQLCALYKQAAFNAQKKVSENNGSNGSFLFYF
LDALSLPIKLENSLIKEEL
>Mature_259_residues
MLKELRQKRPLVHNITNYVVAQFVANGLLALGASPLMSDAIAEMQDLAKISDALAINIGTLNERTILCAKEAIKHYKALN
KPIVLDPVGCSASALRHGTSLELLESEGISVLRGNAAELGSLVGISCGSKGLDSHYATTPIEIVKLVAQKYSVIAVMTGK
TDYVSDGKKVFSITGGSEYLALITGAGCLHAAACASFLSLRKDPLDSMAQLCALYKQAAFNAQKKVSENNGSNGSFLFYF
LDALSLPIKLENSLIKEEL

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=244, Percent_Identity=38.5245901639344, Blast_Score=147, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6325042, Length=277, Percent_Identity=32.8519855595668, Blast_Score=106, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 27577; Mature: 27577

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS00014 ER_TARGET

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKELRQKRPLVHNITNYVVAQFVANGLLALGASPLMSDAIAEMQDLAKISDALAINIGT
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEECC
LNERTILCAKEAIKHYKALNKPIVLDPVGCSASALRHGTSLELLESEGISVLRGNAAELG
CCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHCCCCHHHHHHCCCCEEECCHHHHH
SLVGISCGSKGLDSHYATTPIEIVKLVAQKYSVIAVMTGKTDYVSDGKKVFSITGGSEYL
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEEECCCCCCCCCCEEEEEECCCCEE
ALITGAGCLHAAACASFLSLRKDPLDSMAQLCALYKQAAFNAQKKVSENNGSNGSFLFYF
EEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
LDALSLPIKLENSLIKEEL
HHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLKELRQKRPLVHNITNYVVAQFVANGLLALGASPLMSDAIAEMQDLAKISDALAINIGT
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEECC
LNERTILCAKEAIKHYKALNKPIVLDPVGCSASALRHGTSLELLESEGISVLRGNAAELG
CCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHCCCCHHHHHHCCCCEEECCHHHHH
SLVGISCGSKGLDSHYATTPIEIVKLVAQKYSVIAVMTGKTDYVSDGKKVFSITGGSEYL
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEEECCCCCCCCCCEEEEEECCCCEE
ALITGAGCLHAAACASFLSLRKDPLDSMAQLCALYKQAAFNAQKKVSENNGSNGSFLFYF
EEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
LDALSLPIKLENSLIKEEL
HHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA