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The map label for this gene is thiE

Identifier: 188527310

GI number: 188527310

Start: 501850

End: 502509

Strand: Direct

Name: thiE

Synonym: HPSH_02585

Alternate gene names: 188527310

Gene position: 501850-502509 (Clockwise)

Preceding gene: 188527309

Following gene: 188527320

Centisome position: 31.2

GC content: 41.97

Gene sequence:

>660_bases
TTGTTTGATGCGAATTGTTTGAAACTCATGTTTGTGGCTGGTTCGCAAGACTTCTACCACATAAAGGGCGGCAAAAACGA
TAGGATAAACGCGCTTTTAGACGCTTTAGAATTAGCTTTACAATCTAAAATCACAGCGTTTCAATTCCGCCAGAAAGGCG
ATTTAGCCCTACAAGATCCTATTGAAATCAAACAATTAGCCCTAGAGTGTCAAAAATTATGCCAAAAATACGGCGCGCCT
TTTATTGTCAATGATGAGGTGCAACTCGCGCTAGAATTAAAGGCTGATGGTGTGCATGTGGGGCAAGAAGACATGGCTAT
AGAAGAGGTAATGGCTTTATGCAAAAAGCGCCTTTTTATAGGTTTGAGCGTCAATACTTTAGAGCAAGCCCTAAAAGTGC
GCCATTTAGACGGCGTAGCCTATTTTGGGGTAGGCCCTATTTTTCCCACACAATCTAAAAAAGACAAACAAGTTGTAGGC
GTGGAGCTTTTAAAAAAAATAAAAGATAGCGGGATAAAAAAACCTCTCATAGCGATTGGGGGCATCACGGCGCATAACGC
TTCAAAGTTGCGCGAATATGGGGGTATCGCAGTCATTAGTGCGATCACGCAAGCCAAAGATAAAGCCTTAGCGGTTGGAA
AACTTTTAAAAAATGCGTGA

Upstream 100 bases:

>100_bases
GCTATTTCAAAGGCTAAAGAGCTTTTAACTATCATCATTCAAAACCCTTTAAACATTGGGCATGGGCATGGGCCTTTGAA
TTTATGGAGTATTAAAAAGC

Downstream 100 bases:

>100_bases
GGGATTCTAAAATCCCACAAAAAGCGTTTTTTAAAAAAATCAAGTTTTAGTTTTCTACCCCAGCGTGTCCAATAAACGCT
CTTCAAAGGCAAAAAGCATG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MFDANCLKLMFVAGSQDFYHIKGGKNDRINALLDALELALQSKITAFQFRQKGDLALQDPIEIKQLALECQKLCQKYGAP
FIVNDEVQLALELKADGVHVGQEDMAIEEVMALCKKRLFIGLSVNTLEQALKVRHLDGVAYFGVGPIFPTQSKKDKQVVG
VELLKKIKDSGIKKPLIAIGGITAHNASKLREYGGIAVISAITQAKDKALAVGKLLKNA

Sequences:

>Translated_219_residues
MFDANCLKLMFVAGSQDFYHIKGGKNDRINALLDALELALQSKITAFQFRQKGDLALQDPIEIKQLALECQKLCQKYGAP
FIVNDEVQLALELKADGVHVGQEDMAIEEVMALCKKRLFIGLSVNTLEQALKVRHLDGVAYFGVGPIFPTQSKKDKQVVG
VELLKKIKDSGIKKPLIAIGGITAHNASKLREYGGIAVISAITQAKDKALAVGKLLKNA
>Mature_219_residues
MFDANCLKLMFVAGSQDFYHIKGGKNDRINALLDALELALQSKITAFQFRQKGDLALQDPIEIKQLALECQKLCQKYGAP
FIVNDEVQLALELKADGVHVGQEDMAIEEVMALCKKRLFIGLSVNTLEQALKVRHLDGVAYFGVGPIFPTQSKKDKQVVG
VELLKKIKDSGIKKPLIAIGGITAHNASKLREYGGIAVISAITQAKDKALAVGKLLKNA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=153, Percent_Identity=35.9477124183007, Blast_Score=80, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325042, Length=210, Percent_Identity=32.3809523809524, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23888; Mature: 23888

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDANCLKLMFVAGSQDFYHIKGGKNDRINALLDALELALQSKITAFQFRQKGDLALQDP
CCCCCEEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
IEIKQLALECQKLCQKYGAPFIVNDEVQLALELKADGVHVGQEDMAIEEVMALCKKRLFI
HHHHHHHHHHHHHHHHCCCCEEECCCEEEEEEEECCCEEECHHHHHHHHHHHHHHHHHHC
GLSVNTLEQALKVRHLDGVAYFGVGPIFPTQSKKDKQVVGVELLKKIKDSGIKKPLIAIG
CCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHEEC
GITAHNASKLREYGGIAVISAITQAKDKALAVGKLLKNA
CCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFDANCLKLMFVAGSQDFYHIKGGKNDRINALLDALELALQSKITAFQFRQKGDLALQDP
CCCCCEEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
IEIKQLALECQKLCQKYGAPFIVNDEVQLALELKADGVHVGQEDMAIEEVMALCKKRLFI
HHHHHHHHHHHHHHHHCCCCEEECCCEEEEEEEECCCEEECHHHHHHHHHHHHHHHHHHC
GLSVNTLEQALKVRHLDGVAYFGVGPIFPTQSKKDKQVVGVELLKKIKDSGIKKPLIAIG
CCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHEEC
GITAHNASKLREYGGIAVISAITQAKDKALAVGKLLKNA
CCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA