| Definition | Clostridium botulinum B str. Eklund 17B, complete genome. |
|---|---|
| Accession | NC_010674 |
| Length | 3,800,327 |
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The map label for this gene is rpoN [H]
Identifier: 187934511
GI number: 187934511
Start: 3172777
End: 3174159
Strand: Reverse
Name: rpoN [H]
Synonym: CLL_A3068
Alternate gene names: 187934511
Gene position: 3174159-3172777 (Counterclockwise)
Preceding gene: 187935195
Following gene: 187935546
Centisome position: 83.52
GC content: 25.31
Gene sequence:
>1383_bases ATGAAATTAGATTATGGTATGAAAATTACCCAGGATCAAAAACTTGTATTAACTCAAAGTATGCAACAATCGATAAAACT TTTACAAATGTCCATGCATGATTTAAGGGAGTTCATTGATAATGAATATGCTGAAAATCCAATATTAGAAATTAGTGAAT TAGATAAAGGAATAGAAAATAATACTTCAGAAGATAAATATGATTACAAAAACCTAATAAAGGAATTAGAATCTAATAAT TATAAGAATGAAGTTGATAATAATTATGTAAATAACAAAGAAACAGATTCTACTCCTTGGAATTATATTGAAAAGAGTAA ATCTTTAAATGAGTTTTTACAAGAACAATTAATTGGACTAAATATTGATCCATATTTAGTAGTAGTATCAAAATATATCA TAGAATCCTTAGATAATAGAGGGTATTTGGAAATATCAACAATTCAAATAAGCAATGAACTTAATATAAATCAAGTTAAA GTAGAAGAAGCATTGAAAATTGTACAAGACTTAGAGCCATACGGTATAGGTGCTAGAAATATAGAAGAGTGTTTAATGAT TCAAAGTAAAAAATTAAGTATCTTAGATGATACTATGGAAAAAATAATAAAGAATCATTTAGGAAATATAGCAGATAATA AATATAAGCTAGTAGGTGAAGAATTGGGAATAAACCCTAAAGAAGCTCAAAGGTATGGTGATTTAATTAAAACATTAGAG CCAAAGCCATCTAGAGGATTCTACACAGGAGAACAAGTAAGTTATATAATCCCTGATGCTGAAATAAAAAAAATAGAAAA GCAATATTTTATTATAATGAATGATAACGTATTACCTAAACTTACTATAAATAAAACTTATAAAAGTGTTTTGGAAAATA ATGCAGATGAAAAAACAGTATGTTATGTTAAAGAAAAAATTAATAAAGCTATGTTTTTGATAAAGTCTATTGAACAAAGG AAAAGCACTTTATATAATGTGTTGAAATGCTTATTAAATAAGCAAAGTGAATTTTTTGATAAAGGTTACGAGTACATAAA ACCTTTAACGTTAAAAGAAGTTGCAGAAGAAATAGGAATGCATGAGTCAACTGTTAGTAGGGCTATAAAAGAGAAATATG TACTTACTAGTTATGGAACAATAAAGATAAAATCATTATTTGAAAGTGGATTTTCTTTAAGCAATGAAGGGGATATAGCT ACCAATACTATAAAAAATGAAATTAAAAGGATAGTAGAAGAAGAAAATAAATCTAAACCTTTTTCAGATCAAATTATTTC TGAAATATTAAATAAAGAAAAGATAAATATTTCTAGAAGAACTGTGGCAAAATATAGAGAAGAATTAGGAATTAAATCTT CATCAAAAAGAAAGAGGATTTAA
Upstream 100 bases:
>100_bases TTTAATTAATGAAATCAGAGTATACTATTCTATAGAGAAAAGGTTTTCTATAATTATTATATAATTTTATTACAATATGG TATAAGAAAAGAGGATGTTT
Downstream 100 bases:
>100_bases TAAATTAAATGGTTAATATTTAGAGTGTTAAAGTTTAAATTTAACTTCTAAATATTAATTAATTATTTATATGCTTAAGA TATTTAAAAGTGTTAAAAAT
Product: RNA polymerase factor sigma-54
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 460; Mature: 460
Protein sequence:
>460_residues MKLDYGMKITQDQKLVLTQSMQQSIKLLQMSMHDLREFIDNEYAENPILEISELDKGIENNTSEDKYDYKNLIKELESNN YKNEVDNNYVNNKETDSTPWNYIEKSKSLNEFLQEQLIGLNIDPYLVVVSKYIIESLDNRGYLEISTIQISNELNINQVK VEEALKIVQDLEPYGIGARNIEECLMIQSKKLSILDDTMEKIIKNHLGNIADNKYKLVGEELGINPKEAQRYGDLIKTLE PKPSRGFYTGEQVSYIIPDAEIKKIEKQYFIIMNDNVLPKLTINKTYKSVLENNADEKTVCYVKEKINKAMFLIKSIEQR KSTLYNVLKCLLNKQSEFFDKGYEYIKPLTLKEVAEEIGMHESTVSRAIKEKYVLTSYGTIKIKSLFESGFSLSNEGDIA TNTIKNEIKRIVEEENKSKPFSDQIISEILNKEKINISRRTVAKYREELGIKSSSKRKRI
Sequences:
>Translated_460_residues MKLDYGMKITQDQKLVLTQSMQQSIKLLQMSMHDLREFIDNEYAENPILEISELDKGIENNTSEDKYDYKNLIKELESNN YKNEVDNNYVNNKETDSTPWNYIEKSKSLNEFLQEQLIGLNIDPYLVVVSKYIIESLDNRGYLEISTIQISNELNINQVK VEEALKIVQDLEPYGIGARNIEECLMIQSKKLSILDDTMEKIIKNHLGNIADNKYKLVGEELGINPKEAQRYGDLIKTLE PKPSRGFYTGEQVSYIIPDAEIKKIEKQYFIIMNDNVLPKLTINKTYKSVLENNADEKTVCYVKEKINKAMFLIKSIEQR KSTLYNVLKCLLNKQSEFFDKGYEYIKPLTLKEVAEEIGMHESTVSRAIKEKYVLTSYGTIKIKSLFESGFSLSNEGDIA TNTIKNEIKRIVEEENKSKPFSDQIISEILNKEKINISRRTVAKYREELGIKSSSKRKRI >Mature_460_residues MKLDYGMKITQDQKLVLTQSMQQSIKLLQMSMHDLREFIDNEYAENPILEISELDKGIENNTSEDKYDYKNLIKELESNN YKNEVDNNYVNNKETDSTPWNYIEKSKSLNEFLQEQLIGLNIDPYLVVVSKYIIESLDNRGYLEISTIQISNELNINQVK VEEALKIVQDLEPYGIGARNIEECLMIQSKKLSILDDTMEKIIKNHLGNIADNKYKLVGEELGINPKEAQRYGDLIKTLE PKPSRGFYTGEQVSYIIPDAEIKKIEKQYFIIMNDNVLPKLTINKTYKSVLENNADEKTVCYVKEKINKAMFLIKSIEQR KSTLYNVLKCLLNKQSEFFDKGYEYIKPLTLKEVAEEIGMHESTVSRAIKEKYVLTSYGTIKIKSLFESGFSLSNEGDIA TNTIKNEIKRIVEEENKSKPFSDQIISEILNKEKINISRRTVAKYREELGIKSSSKRKRI
Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]
COG id: COG1508
COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the sigma-54 factor family [H]
Homologues:
Organism=Escherichia coli, GI1789594, Length=485, Percent_Identity=28.4536082474227, Blast_Score=236, Evalue=2e-63,
Paralogues:
None
Copy number: 70 (log & stationary phase) [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR000394 - InterPro: IPR007046 - InterPro: IPR007634 [H]
Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]
EC number: NA
Molecular weight: Translated: 53315; Mature: 53315
Theoretical pI: Translated: 5.54; Mature: 5.54
Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 2.2 %Met (Mature Protein) 2.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKLDYGMKITQDQKLVLTQSMQQSIKLLQMSMHDLREFIDNEYAENPILEISELDKGIEN CCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCC NTSEDKYDYKNLIKELESNNYKNEVDNNYVNNKETDSTPWNYIEKSKSLNEFLQEQLIGL CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC NIDPYLVVVSKYIIESLDNRGYLEISTIQISNELNINQVKVEEALKIVQDLEPYGIGARN CCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCC IEECLMIQSKKLSILDDTMEKIIKNHLGNIADNKYKLVGEELGINPKEAQRYGDLIKTLE HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHCCCHHHHHHHHHHHHHCC PKPSRGFYTGEQVSYIIPDAEIKKIEKQYFIIMNDNVLPKLTINKTYKSVLENNADEKTV CCCCCCCCCCCCEEEECCCHHHHHHCHHEEEEECCCCCCEEEHHHHHHHHHHCCCCCHHH CYVKEKINKAMFLIKSIEQRKSTLYNVLKCLLNKQSEFFDKGYEYIKPLTLKEVAEEIGM HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCC HESTVSRAIKEKYVLTSYGTIKIKSLFESGFSLSNEGDIATNTIKNEIKRIVEEENKSKP CHHHHHHHHHHHHEEECCCCEEHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC FSDQIISEILNKEKINISRRTVAKYREELGIKSSSKRKRI HHHHHHHHHHCHHHCCHHHHHHHHHHHHHCCCCCCCCCCC >Mature Secondary Structure MKLDYGMKITQDQKLVLTQSMQQSIKLLQMSMHDLREFIDNEYAENPILEISELDKGIEN CCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCC NTSEDKYDYKNLIKELESNNYKNEVDNNYVNNKETDSTPWNYIEKSKSLNEFLQEQLIGL CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC NIDPYLVVVSKYIIESLDNRGYLEISTIQISNELNINQVKVEEALKIVQDLEPYGIGARN CCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCC IEECLMIQSKKLSILDDTMEKIIKNHLGNIADNKYKLVGEELGINPKEAQRYGDLIKTLE HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHCCCHHHHHHHHHHHHHCC PKPSRGFYTGEQVSYIIPDAEIKKIEKQYFIIMNDNVLPKLTINKTYKSVLENNADEKTV CCCCCCCCCCCCEEEECCCHHHHHHCHHEEEEECCCCCCEEEHHHHHHHHHHCCCCCHHH CYVKEKINKAMFLIKSIEQRKSTLYNVLKCLLNKQSEFFDKGYEYIKPLTLKEVAEEIGM HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCC HESTVSRAIKEKYVLTSYGTIKIKSLFESGFSLSNEGDIATNTIKNEIKRIVEEENKSKP CHHHHHHHHHHHHEEECCCCEEHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC FSDQIISEILNKEKINISRRTVAKYREELGIKSSSKRKRI HHHHHHHHHHCHHHCCHHHHHHHHHHHHHCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: DNA [C]
Specific reaction: Protein + DNA = Protein-DNA [C]
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8550525 [H]