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The map label for this gene is yclM [H]

Identifier: 187933875

GI number: 187933875

Start: 1066249

End: 1067559

Strand: Reverse

Name: yclM [H]

Synonym: CLL_A1047

Alternate gene names: 187933875

Gene position: 1067559-1066249 (Counterclockwise)

Preceding gene: 187935467

Following gene: 187934926

Centisome position: 28.09

GC content: 28.83

Gene sequence:

>1311_bases
ATGAATACTATTATAACAAAATTTGGTGGAAGTTCACTAGCTGATGCTGAACAATTCAAAAAGGTGAAAAATATAATTAC
ATCTAATAAATCTAGAAAATTTGTTATTCCATCAGCACCAGGAAAGAGAACTTCTAAAGACTCTAAAATAACTGATTTAT
TATATCTTTGCCATGCTCATATCAATACGGGTATATCATTAGATGATGTTTTTAATCATATAAATTCTAGATATATTGGT
ATCGTAAATGACCTTAAATTAGATCTAAATATCAAATCATACTTAGACCTAATAAAAAAAGATATAGAGAATGGTGCATC
TAGTGATTATGCAGCTAGTAGAGGGGAGTATTTAAATGCTATAATCCTTAGCAACTATTTAGGCTTTGAATTTATAGATG
CTAAAGATGTAATTAAATTTAATACTGATGGATGTTTAAATTATGATGAAACATTAAGATTATTAAAAGAAAAGCTTTCT
TCAATAGATAAAGCTGTCATTCCAGGGTTTTATGGTTCAGATAACAATGGAGATATTGTTACTTTTTCAAGGGGTGGATC
AGACGTTACTGGTGCTTTAGTTACTGCAAGTTTAAACGCTGATCTTTATGAAAATTGGACAGATGTTTCTGGTTTCTTAA
TGGCTGATCCTAGAATTATTTCTAATCCTAAGCCTATAAGTACAATAACTTATACTGAACTTAGAGAACTTTCTTATATG
GGTGCTTCTGTTTTACATGAAGAAGCTATTTTTCCAGTAAGAAAAGCTGGGATTCCAATTAATATCAAAAATACAAACAA
ACCAGAAGATAAGGGAACTTTTATAGTAGAAGACTCATTTAATGATGATTCACAAATTATAACTGGTATAGCTGGTAAAA
AAGATTTTACTGTAATTTCAATTGCAAAAGCTTTAATGAATTCTGAATTAGGTTTTTGTAGAAAAATATTATCAATACTT
GAACAGCATAATGTTTCATTTGAAAATATGCCTTCTGGTATAGACACTGTATGTTTGGTAATTTCAGATTCTCAGTTAAA
AAATAAAACTGCTTTAATTGTGGAAGAAATTAAAAGAACTTGTAATCCTGATACAATAGAAGTTCACCCTAACATGGCAT
TGATAGCTACTGTTGGTAAAGGTATGGCTAAACAAAAAGGTGTAGCATCAAAAGTATTTACAGCATTATCAAAAGCTAAT
GTAAATATTCGAATGATTGATCAAGGATCTAGTGAAATCAACATATTAGTTGGTATAGAAAATGATAATTTTGAAAAAGC
CATAGCTGCTATTTATAATGCTTTTAATTAA

Upstream 100 bases:

>100_bases
TACAATTTATTATGTATAAAACTATATACTAAAATATATTTAGTAATATTAAAATGCTATTACTAAATATAATTTTTATT
TTTTTGGGGGGGCTATAAAA

Downstream 100 bases:

>100_bases
TATTTAAAACAACATTTAATATTATAGTATAAATAGATTCATAGCTATCGAGATAATTATCCTTTTATAAAAAATCACTT
TAAGAAATCTAACAATAAAA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MNTIITKFGGSSLADAEQFKKVKNIITSNKSRKFVIPSAPGKRTSKDSKITDLLYLCHAHINTGISLDDVFNHINSRYIG
IVNDLKLDLNIKSYLDLIKKDIENGASSDYAASRGEYLNAIILSNYLGFEFIDAKDVIKFNTDGCLNYDETLRLLKEKLS
SIDKAVIPGFYGSDNNGDIVTFSRGGSDVTGALVTASLNADLYENWTDVSGFLMADPRIISNPKPISTITYTELRELSYM
GASVLHEEAIFPVRKAGIPINIKNTNKPEDKGTFIVEDSFNDDSQIITGIAGKKDFTVISIAKALMNSELGFCRKILSIL
EQHNVSFENMPSGIDTVCLVISDSQLKNKTALIVEEIKRTCNPDTIEVHPNMALIATVGKGMAKQKGVASKVFTALSKAN
VNIRMIDQGSSEINILVGIENDNFEKAIAAIYNAFN

Sequences:

>Translated_436_residues
MNTIITKFGGSSLADAEQFKKVKNIITSNKSRKFVIPSAPGKRTSKDSKITDLLYLCHAHINTGISLDDVFNHINSRYIG
IVNDLKLDLNIKSYLDLIKKDIENGASSDYAASRGEYLNAIILSNYLGFEFIDAKDVIKFNTDGCLNYDETLRLLKEKLS
SIDKAVIPGFYGSDNNGDIVTFSRGGSDVTGALVTASLNADLYENWTDVSGFLMADPRIISNPKPISTITYTELRELSYM
GASVLHEEAIFPVRKAGIPINIKNTNKPEDKGTFIVEDSFNDDSQIITGIAGKKDFTVISIAKALMNSELGFCRKILSIL
EQHNVSFENMPSGIDTVCLVISDSQLKNKTALIVEEIKRTCNPDTIEVHPNMALIATVGKGMAKQKGVASKVFTALSKAN
VNIRMIDQGSSEINILVGIENDNFEKAIAAIYNAFN
>Mature_436_residues
MNTIITKFGGSSLADAEQFKKVKNIITSNKSRKFVIPSAPGKRTSKDSKITDLLYLCHAHINTGISLDDVFNHINSRYIG
IVNDLKLDLNIKSYLDLIKKDIENGASSDYAASRGEYLNAIILSNYLGFEFIDAKDVIKFNTDGCLNYDETLRLLKEKLS
SIDKAVIPGFYGSDNNGDIVTFSRGGSDVTGALVTASLNADLYENWTDVSGFLMADPRIISNPKPISTITYTELRELSYM
GASVLHEEAIFPVRKAGIPINIKNTNKPEDKGTFIVEDSFNDDSQIITGIAGKKDFTVISIAKALMNSELGFCRKILSIL
EQHNVSFENMPSGIDTVCLVISDSQLKNKTALIVEEIKRTCNPDTIEVHPNMALIATVGKGMAKQKGVASKVFTALSKAN
VNIRMIDQGSSEINILVGIENDNFEKAIAAIYNAFN

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=459, Percent_Identity=29.8474945533769, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1790455, Length=463, Percent_Identity=24.622030237581, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1790376, Length=290, Percent_Identity=24.1379310344828, Blast_Score=73, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320893, Length=491, Percent_Identity=26.0692464358452, Blast_Score=121, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 47807; Mature: 47807

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTIITKFGGSSLADAEQFKKVKNIITSNKSRKFVIPSAPGKRTSKDSKITDLLYLCHAH
CCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHH
INTGISLDDVFNHINSRYIGIVNDLKLDLNIKSYLDLIKKDIENGASSDYAASRGEYLNA
HCCCCCHHHHHHHHCCCEEEEEECEEEEEEHHHHHHHHHHHHHCCCCCCCHHCCCHHHHH
IILSNYLGFEFIDAKDVIKFNTDGCLNYDETLRLLKEKLSSIDKAVIPGFYGSDNNGDIV
HHHHHCCCCEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
TFSRGGSDVTGALVTASLNADLYENWTDVSGFLMADPRIISNPKPISTITYTELRELSYM
EEECCCCCCCCEEEEEECCCHHHCCCCCCCCEEEECCEEECCCCCCCEEEHHHHHHHHHH
GASVLHEEAIFPVRKAGIPINIKNTNKPEDKGTFIVEDSFNDDSQIITGIAGKKDFTVIS
HHHHHHHHHCCCHHHCCCEEEECCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCHHHH
IAKALMNSELGFCRKILSILEQHNVSFENMPSGIDTVCLVISDSQLKNKTALIVEEIKRT
HHHHHHCCCHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
CNPDTIEVHPNMALIATVGKGMAKQKGVASKVFTALSKANVNIRMIDQGSSEINILVGIE
CCCCEEEECCCEEEEEECCCCHHHHCCHHHHHHHHHHHCCCEEEEEECCCCEEEEEEEEC
NDNFEKAIAAIYNAFN
CCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNTIITKFGGSSLADAEQFKKVKNIITSNKSRKFVIPSAPGKRTSKDSKITDLLYLCHAH
CCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHH
INTGISLDDVFNHINSRYIGIVNDLKLDLNIKSYLDLIKKDIENGASSDYAASRGEYLNA
HCCCCCHHHHHHHHCCCEEEEEECEEEEEEHHHHHHHHHHHHHCCCCCCCHHCCCHHHHH
IILSNYLGFEFIDAKDVIKFNTDGCLNYDETLRLLKEKLSSIDKAVIPGFYGSDNNGDIV
HHHHHCCCCEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
TFSRGGSDVTGALVTASLNADLYENWTDVSGFLMADPRIISNPKPISTITYTELRELSYM
EEECCCCCCCCEEEEEECCCHHHCCCCCCCCEEEECCEEECCCCCCCEEEHHHHHHHHHH
GASVLHEEAIFPVRKAGIPINIKNTNKPEDKGTFIVEDSFNDDSQIITGIAGKKDFTVIS
HHHHHHHHHCCCHHHCCCEEEECCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCHHHH
IAKALMNSELGFCRKILSILEQHNVSFENMPSGIDTVCLVISDSQLKNKTALIVEEIKRT
HHHHHHCCCHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
CNPDTIEVHPNMALIATVGKGMAKQKGVASKVFTALSKANVNIRMIDQGSSEINILVGIE
CCCCEEEECCCEEEEEECCCCHHHHCCHHHHHHHHHHHCCCEEEEEECCCCEEEEEEEEC
NDNFEKAIAAIYNAFN
CCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]