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The map label for this gene is gidA [H]

Identifier: 187933395

GI number: 187933395

Start: 3794142

End: 3796022

Strand: Reverse

Name: gidA [H]

Synonym: CLL_A3599

Alternate gene names: 187933395

Gene position: 3796022-3794142 (Counterclockwise)

Preceding gene: 187932414

Following gene: 187933315

Centisome position: 99.89

GC content: 30.52

Gene sequence:

>1881_bases
ATGAAATATAATTATGAAGCAGGGGAAGTTGATGTAGTAGTCGTTGGTGCTGGGCATGCAGGATGTGAAGCAGCATTAGC
ATCTGCTAGAATGGGATTAAATACTTTAATTTGTACTATAAATTTAGATTCAATAGCTATGATGCCATGTAATCCTAATA
TAGGGGGAACAGCTAAAGGTCACTTAGTGAGAGAAATTGATGCATTAGGTGGAGAAATGGGTGTGAATATAGATCATACA
TTCATTCAATCAAGAATGTTAAACACCTCTAAAGGACCAGCAGTTCATTCATTAAGAGCACAAGCTGATAAAAAGGACTA
TCAATTTAGAATGAAGAGAGTATTAGAAGAGCAAGAAAATCTTCAAATAAGACAAATTGAGGTTACTGAGCTTTTAGTTG
AAGACAATAAGATAACAGGAGTTATTACTAAAAATGGAGCAATTTTTAGAGCTAAAGCTGTAATTTTATCTACAGGTACA
TATTTAAAAGGAAAGATAATAATAGGGGAAGTTAGTTATTCAGGAGGTCCAAATGGATTATTCCCAGCTAATGATTTATC
ACAATCTTTATTAGATTTAGGAATTTCATTAAGAAGATTTAAAACTGGAACTCCAGCAAGAATTAATAGAAGATCTGTAG
ATTTTTCTAAAATGATTGAACAACCAGGTGATGATAAAATAGTACCTTTCTCATTTATGAGTGGAAATATAGATAAAGAA
CAAATATCTTGTTATTTAACATACACTAATGAACAAACTCATAATGTGATTAAGGAAAACATAGGAAGATCACCAATATA
TAATGGAAGTATTAAGGGAGTAGGTCCTAGATATTGTCCATCAATTGAAGATAAAGTAATGAGATTCCCTGATAAATTAC
AACATCAAATATTTGTTGAACCAGAAGGTTTAGACACTCTAGAAATGTATGTAGGGGGGATGTCTTCATCTTTACCAGAA
GAAGTTCAATTGAAAATGATGAGAACATTACCTGGATTAGAAAATGTAGAAATAATGAGAACTGCATACGCAATTGAATA
TGATTCTGTAGACCCTACTGAACTAGAGCCAACATTAGAATTTAAAAATGTTGAAGGTTTATATGGTGCTGGTCAATTTA
ATGGTAGTTCAGGATATGAAGAGGCAGGAGCACAAGGTCTAGTAGCAGGTATAAATGCTGCATTAAAGATTAAAGGTGAA
GAACCGATGATATTAACTAGATCAGATGCATATATTGGCGTTCTTATAGATGATTTAGTTACTAAGGGTACTAATGAACC
ATATAGAATGATGACATCTAGAGCTGAATATAGATTAATTTTAAGACAAGATAATGCAGACTTTAGATTAACTGAGATAG
GAAAAAAGGTAGGATTAGTTACAGAAGAAAGATATGAGACTTTCTTAAATAGAAAAAATACAATTGAAAATGAATTAGAA
AGATTAAAAAAATTACAAATAACTAATAAAAGAGAAACAAATGAATTTTTATTGGCATCGGGATCAGCTGAATTAAAAAA
ACCTATAAGTTTTTATGAATTAATAAAAAGACCAGAAGTTGATTATTTTAGTTTAAAAGATTTAGATCCGGAAAGAGAAG
AACTTTCTGAAGATATTGGTGAACAAATAAACATAATTGCTAAATATGAAGGTTATATAAGTAATCAACTTGAACAAGTA
GCTCAATTTAGAAAATTTGAGAAGAAACTATTACCTAAGGATATAGACTATAGCAATGTAAAAGGATTAAGGACAGAAGC
TGAACAAAAATTAAATAGTATAAAACCTATAAGTATAGGTCAGGCATCACGTATTTCAGGGGTATCTCCAGCAGATATAT
CTGTATTACTTATTTATCTTGAACATTACTATAATAAATAA

Upstream 100 bases:

>100_bases
AGCAATGAAAGCGCTTGGTGAAATAACAGGTGATGAACTAGAAGAGGATGTATTAAACAAAATTTTTAGTGAATTTTGTG
TAGGAAAGTAGGAATGGTAT

Downstream 100 bases:

>100_bases
TTATTGGAGGTATTTATGAAATTTTATGATTTAATGTGCAAAGCGGCACAAGATGTAGGGTTAGAATTATCAAAAGAGCA
ATATGAGAAGTTTATAATAT

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MKYNYEAGEVDVVVVGAGHAGCEAALASARMGLNTLICTINLDSIAMMPCNPNIGGTAKGHLVREIDALGGEMGVNIDHT
FIQSRMLNTSKGPAVHSLRAQADKKDYQFRMKRVLEEQENLQIRQIEVTELLVEDNKITGVITKNGAIFRAKAVILSTGT
YLKGKIIIGEVSYSGGPNGLFPANDLSQSLLDLGISLRRFKTGTPARINRRSVDFSKMIEQPGDDKIVPFSFMSGNIDKE
QISCYLTYTNEQTHNVIKENIGRSPIYNGSIKGVGPRYCPSIEDKVMRFPDKLQHQIFVEPEGLDTLEMYVGGMSSSLPE
EVQLKMMRTLPGLENVEIMRTAYAIEYDSVDPTELEPTLEFKNVEGLYGAGQFNGSSGYEEAGAQGLVAGINAALKIKGE
EPMILTRSDAYIGVLIDDLVTKGTNEPYRMMTSRAEYRLILRQDNADFRLTEIGKKVGLVTEERYETFLNRKNTIENELE
RLKKLQITNKRETNEFLLASGSAELKKPISFYELIKRPEVDYFSLKDLDPEREELSEDIGEQINIIAKYEGYISNQLEQV
AQFRKFEKKLLPKDIDYSNVKGLRTEAEQKLNSIKPISIGQASRISGVSPADISVLLIYLEHYYNK

Sequences:

>Translated_626_residues
MKYNYEAGEVDVVVVGAGHAGCEAALASARMGLNTLICTINLDSIAMMPCNPNIGGTAKGHLVREIDALGGEMGVNIDHT
FIQSRMLNTSKGPAVHSLRAQADKKDYQFRMKRVLEEQENLQIRQIEVTELLVEDNKITGVITKNGAIFRAKAVILSTGT
YLKGKIIIGEVSYSGGPNGLFPANDLSQSLLDLGISLRRFKTGTPARINRRSVDFSKMIEQPGDDKIVPFSFMSGNIDKE
QISCYLTYTNEQTHNVIKENIGRSPIYNGSIKGVGPRYCPSIEDKVMRFPDKLQHQIFVEPEGLDTLEMYVGGMSSSLPE
EVQLKMMRTLPGLENVEIMRTAYAIEYDSVDPTELEPTLEFKNVEGLYGAGQFNGSSGYEEAGAQGLVAGINAALKIKGE
EPMILTRSDAYIGVLIDDLVTKGTNEPYRMMTSRAEYRLILRQDNADFRLTEIGKKVGLVTEERYETFLNRKNTIENELE
RLKKLQITNKRETNEFLLASGSAELKKPISFYELIKRPEVDYFSLKDLDPEREELSEDIGEQINIIAKYEGYISNQLEQV
AQFRKFEKKLLPKDIDYSNVKGLRTEAEQKLNSIKPISIGQASRISGVSPADISVLLIYLEHYYNK
>Mature_626_residues
MKYNYEAGEVDVVVVGAGHAGCEAALASARMGLNTLICTINLDSIAMMPCNPNIGGTAKGHLVREIDALGGEMGVNIDHT
FIQSRMLNTSKGPAVHSLRAQADKKDYQFRMKRVLEEQENLQIRQIEVTELLVEDNKITGVITKNGAIFRAKAVILSTGT
YLKGKIIIGEVSYSGGPNGLFPANDLSQSLLDLGISLRRFKTGTPARINRRSVDFSKMIEQPGDDKIVPFSFMSGNIDKE
QISCYLTYTNEQTHNVIKENIGRSPIYNGSIKGVGPRYCPSIEDKVMRFPDKLQHQIFVEPEGLDTLEMYVGGMSSSLPE
EVQLKMMRTLPGLENVEIMRTAYAIEYDSVDPTELEPTLEFKNVEGLYGAGQFNGSSGYEEAGAQGLVAGINAALKIKGE
EPMILTRSDAYIGVLIDDLVTKGTNEPYRMMTSRAEYRLILRQDNADFRLTEIGKKVGLVTEERYETFLNRKNTIENELE
RLKKLQITNKRETNEFLLASGSAELKKPISFYELIKRPEVDYFSLKDLDPEREELSEDIGEQINIIAKYEGYISNQLEQV
AQFRKFEKKLLPKDIDYSNVKGLRTEAEQKLNSIKPISIGQASRISGVSPADISVLLIYLEHYYNK

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=630, Percent_Identity=40.6349206349206, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI19882217, Length=655, Percent_Identity=39.0839694656489, Blast_Score=445, Evalue=1e-125,
Organism=Homo sapiens, GI183227703, Length=670, Percent_Identity=38.2089552238806, Blast_Score=440, Evalue=1e-123,
Organism=Escherichia coli, GI2367273, Length=614, Percent_Identity=52.7687296416938, Blast_Score=632, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=618, Percent_Identity=40.7766990291262, Blast_Score=475, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6321202, Length=623, Percent_Identity=43.4991974317817, Blast_Score=496, Evalue=1e-141,
Organism=Drosophila melanogaster, GI24658174, Length=630, Percent_Identity=41.7460317460317, Blast_Score=455, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 69986; Mature: 69986

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYNYEAGEVDVVVVGAGHAGCEAALASARMGLNTLICTINLDSIAMMPCNPNIGGTAKG
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEEEECCEEEECCCCCCCCCCCC
HLVREIDALGGEMGVNIDHTFIQSRMLNTSKGPAVHSLRAQADKKDYQFRMKRVLEEQEN
HHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHC
LQIRQIEVTELLVEDNKITGVITKNGAIFRAKAVILSTGTYLKGKIIIGEVSYSGGPNGL
CEEEEEEEEEEEEECCEEEEEEECCCCEEEEEEEEEECCCEEEEEEEEEEEECCCCCCCC
FPANDLSQSLLDLGISLRRFKTGTPARINRRSVDFSKMIEQPGDDKIVPFSFMSGNIDKE
CCCHHHHHHHHHHCCHHEEECCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCCHH
QISCYLTYTNEQTHNVIKENIGRSPIYNGSIKGVGPRYCPSIEDKVMRFPDKLQHQIFVE
HEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHCCEEEEC
PEGLDTLEMYVGGMSSSLPEEVQLKMMRTLPGLENVEIMRTAYAIEYDSVDPTELEPTLE
CCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHEEEEEEEEEECCCCCCCCCCCEE
FKNVEGLYGAGQFNGSSGYEEAGAQGLVAGINAALKIKGEEPMILTRSDAYIGVLIDDLV
ECCCCCEECCCCCCCCCCCHHHCCCCEEECCCEEEEECCCCCEEEEECCCEEEEEHHHHH
TKGTNEPYRMMTSRAEYRLILRQDNADFRLTEIGKKVGLVTEERYETFLNRKNTIENELE
HCCCCCHHHHHHCCCCEEEEEEECCCCEEHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHH
RLKKLQITNKRETNEFLLASGSAELKKPISFYELIKRPEVDYFSLKDLDPEREELSEDIG
HHHHHHCCCCCCCCCEEEECCCHHHHCCHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHH
EQINIIAKYEGYISNQLEQVAQFRKFEKKLLPKDIDYSNVKGLRTEAEQKLNSIKPISIG
HHEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
QASRISGVSPADISVLLIYLEHYYNK
CCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYNYEAGEVDVVVVGAGHAGCEAALASARMGLNTLICTINLDSIAMMPCNPNIGGTAKG
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEEEECCEEEECCCCCCCCCCCC
HLVREIDALGGEMGVNIDHTFIQSRMLNTSKGPAVHSLRAQADKKDYQFRMKRVLEEQEN
HHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHC
LQIRQIEVTELLVEDNKITGVITKNGAIFRAKAVILSTGTYLKGKIIIGEVSYSGGPNGL
CEEEEEEEEEEEEECCEEEEEEECCCCEEEEEEEEEECCCEEEEEEEEEEEECCCCCCCC
FPANDLSQSLLDLGISLRRFKTGTPARINRRSVDFSKMIEQPGDDKIVPFSFMSGNIDKE
CCCHHHHHHHHHHCCHHEEECCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCCHH
QISCYLTYTNEQTHNVIKENIGRSPIYNGSIKGVGPRYCPSIEDKVMRFPDKLQHQIFVE
HEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHCCEEEEC
PEGLDTLEMYVGGMSSSLPEEVQLKMMRTLPGLENVEIMRTAYAIEYDSVDPTELEPTLE
CCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHEEEEEEEEEECCCCCCCCCCCEE
FKNVEGLYGAGQFNGSSGYEEAGAQGLVAGINAALKIKGEEPMILTRSDAYIGVLIDDLV
ECCCCCEECCCCCCCCCCCHHHCCCCEEECCCEEEEECCCCCEEEEECCCEEEEEHHHHH
TKGTNEPYRMMTSRAEYRLILRQDNADFRLTEIGKKVGLVTEERYETFLNRKNTIENELE
HCCCCCHHHHHHCCCCEEEEEEECCCCEEHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHH
RLKKLQITNKRETNEFLLASGSAELKKPISFYELIKRPEVDYFSLKDLDPEREELSEDIG
HHHHHHCCCCCCCCCEEEECCCHHHHCCHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHH
EQINIIAKYEGYISNQLEQVAQFRKFEKKLLPKDIDYSNVKGLRTEAEQKLNSIKPISIG
HHEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
QASRISGVSPADISVLLIYLEHYYNK
CCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA