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The map label for this gene is trmE [H]

Identifier: 187932414

GI number: 187932414

Start: 3796032

End: 3797402

Strand: Reverse

Name: trmE [H]

Synonym: CLL_A3600

Alternate gene names: 187932414

Gene position: 3797402-3796032 (Counterclockwise)

Preceding gene: 187935358

Following gene: 187933395

Centisome position: 99.92

GC content: 29.18

Gene sequence:

>1371_bases
ATGAGAGAATTTGATACCATTTGTGGTATAGCAACCCCTATTGGTGAGGGTGGAGTTTCTATTATTAGAATTTCTGGAAG
TAAAGCATTAGATATAATTAGCAAAATATTTGTTGGTAAAAATAATATAGATTTAAAACAAATGAAAACTTATACTATGA
GATACGGACACATTATAGAGCTTGAGAGTAAGGATGTAATTGATGAGGTTATTATAAGCTATATGAAGGGACCTCATAGC
TATACTACTGAGGATATAATAGAAATCAACTGTCATGGTGGTGTTATATCAACAAATAGTGTAATGAATCAGGTTATTAA
AGCAGGTGTAAGAGTTGCAGAACCAGGAGAATTTACAAAGAGAGCATTTTTAAACGGTAGAATTGACTTAAGTCAAGCTG
AAGCCGTTATTGATATAATAAAAGCTAAAACAGATTTGTCCATGAAGTCAGCACTTATGCAAAGTGGTGGAGCTTTATCA
AAGCAAATTAAGGAAATAAGACAATACTTATTAAACACCTTAGCATTAATTGAGTATGGAGTAGATTTTACGGAAGACGA
TGAAGACATCGATGATACTTTAGTTTTAAGAGTTAAAGATGGAATAAAAACTACTATTTTAAAAGTTAAGGAACTATTAA
AAGGTGCAGATGAAGGTAAAATAATTAGAGATGGTCTTAATGTTGTTATTATCGGAAAGCCTAATGTAGGAAAATCATCT
TTACTTAATGTTTTACTAAAAGAAAAAAGAGCAATAGTAACAGATGTTCCTGGTACTACAAGAGATATAATAGAAGAGTA
TTTAAATATAGATGGTATACCTATAAAAATAACAGATACTGCAGGTATAAGAGAGACTGAAGACACTGTTGAGAAGATAG
GTGTTGAAAGATCTAGAGAAAAGATTGAAGAAGCAGATTTAATTATTTTGATATTAGATTCTTCTAGAGAATTAGAAGAA
GAAGATAAGGAAATAATTAATACTATAAAAGACAAGAATCACATAGTATTATTAAATAAAACGGATTTAGATAGAAGAAT
AGCAGATATTGATTTGGATAATCAAATAAATATTTCAGCTAAAACTGGATACGGAATTGAAGAACTAAAAAATAAAATAA
AAGAGTTATTTTTTAGTGGAGATATAAATAGTGAAAGTTTAATAGTATCTAATGTTAGACATAAGCAAGCATTATACAGA
TCGTTAGAAAACTGTGAGATAGCATTAGATAGGGTTAATGCTAATGAATTTTTAGATTTAATTTCTATTTATGTAACTTC
AGCAATGAAAGCGCTTGGTGAAATAACAGGTGATGAACTAGAAGAGGATGTATTAAACAAAATTTTTAGTGAATTTTGTG
TAGGAAAGTAG

Upstream 100 bases:

>100_bases
TAAAATAAAGCTAATGCATGTGACTTTGACTAATTCCATATTCATTGGTTATAATAATACAAATAAATCACAAAATTAAT
AACATGAGGAGAATCAGATT

Downstream 100 bases:

>100_bases
GAATGGTATATGAAATATAATTATGAAGCAGGGGAAGTTGATGTAGTAGTCGTTGGTGCTGGGCATGCAGGATGTGAAGC
AGCATTAGCATCTGCTAGAA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MREFDTICGIATPIGEGGVSIIRISGSKALDIISKIFVGKNNIDLKQMKTYTMRYGHIIELESKDVIDEVIISYMKGPHS
YTTEDIIEINCHGGVISTNSVMNQVIKAGVRVAEPGEFTKRAFLNGRIDLSQAEAVIDIIKAKTDLSMKSALMQSGGALS
KQIKEIRQYLLNTLALIEYGVDFTEDDEDIDDTLVLRVKDGIKTTILKVKELLKGADEGKIIRDGLNVVIIGKPNVGKSS
LLNVLLKEKRAIVTDVPGTTRDIIEEYLNIDGIPIKITDTAGIRETEDTVEKIGVERSREKIEEADLIILILDSSRELEE
EDKEIINTIKDKNHIVLLNKTDLDRRIADIDLDNQINISAKTGYGIEELKNKIKELFFSGDINSESLIVSNVRHKQALYR
SLENCEIALDRVNANEFLDLISIYVTSAMKALGEITGDELEEDVLNKIFSEFCVGK

Sequences:

>Translated_456_residues
MREFDTICGIATPIGEGGVSIIRISGSKALDIISKIFVGKNNIDLKQMKTYTMRYGHIIELESKDVIDEVIISYMKGPHS
YTTEDIIEINCHGGVISTNSVMNQVIKAGVRVAEPGEFTKRAFLNGRIDLSQAEAVIDIIKAKTDLSMKSALMQSGGALS
KQIKEIRQYLLNTLALIEYGVDFTEDDEDIDDTLVLRVKDGIKTTILKVKELLKGADEGKIIRDGLNVVIIGKPNVGKSS
LLNVLLKEKRAIVTDVPGTTRDIIEEYLNIDGIPIKITDTAGIRETEDTVEKIGVERSREKIEEADLIILILDSSRELEE
EDKEIINTIKDKNHIVLLNKTDLDRRIADIDLDNQINISAKTGYGIEELKNKIKELFFSGDINSESLIVSNVRHKQALYR
SLENCEIALDRVNANEFLDLISIYVTSAMKALGEITGDELEEDVLNKIFSEFCVGK
>Mature_456_residues
MREFDTICGIATPIGEGGVSIIRISGSKALDIISKIFVGKNNIDLKQMKTYTMRYGHIIELESKDVIDEVIISYMKGPHS
YTTEDIIEINCHGGVISTNSVMNQVIKAGVRVAEPGEFTKRAFLNGRIDLSQAEAVIDIIKAKTDLSMKSALMQSGGALS
KQIKEIRQYLLNTLALIEYGVDFTEDDEDIDDTLVLRVKDGIKTTILKVKELLKGADEGKIIRDGLNVVIIGKPNVGKSS
LLNVLLKEKRAIVTDVPGTTRDIIEEYLNIDGIPIKITDTAGIRETEDTVEKIGVERSREKIEEADLIILILDSSRELEE
EDKEIINTIKDKNHIVLLNKTDLDRRIADIDLDNQINISAKTGYGIEELKNKIKELFFSGDINSESLIVSNVRHKQALYR
SLENCEIALDRVNANEFLDLISIYVTSAMKALGEITGDELEEDVLNKIFSEFCVGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=468, Percent_Identity=30.7692307692308, Blast_Score=233, Evalue=4e-61,
Organism=Homo sapiens, GI193082988, Length=499, Percent_Identity=30.060120240481, Blast_Score=222, Evalue=7e-58,
Organism=Homo sapiens, GI193082993, Length=457, Percent_Identity=29.9781181619256, Blast_Score=213, Evalue=3e-55,
Organism=Escherichia coli, GI2367268, Length=467, Percent_Identity=35.5460385438972, Blast_Score=286, Evalue=3e-78,
Organism=Escherichia coli, GI87082120, Length=170, Percent_Identity=32.9411764705882, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17507259, Length=462, Percent_Identity=28.5714285714286, Blast_Score=192, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6323665, Length=503, Percent_Identity=29.4234592445328, Blast_Score=189, Evalue=7e-49,
Organism=Drosophila melanogaster, GI45550813, Length=465, Percent_Identity=33.5483870967742, Blast_Score=245, Evalue=5e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 50761; Mature: 50761

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREFDTICGIATPIGEGGVSIIRISGSKALDIISKIFVGKNNIDLKQMKTYTMRYGHIIE
CCCHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEE
LESKDVIDEVIISYMKGPHSYTTEDIIEINCHGGVISTNSVMNQVIKAGVRVAEPGEFTK
ECCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHCCCEECCCCHHHH
RAFLNGRIDLSQAEAVIDIIKAKTDLSMKSALMQSGGALSKQIKEIRQYLLNTLALIEYG
HHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
VDFTEDDEDIDDTLVLRVKDGIKTTILKVKELLKGADEGKIIRDGLNVVIIGKPNVGKSS
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCCHHH
LLNVLLKEKRAIVTDVPGTTRDIIEEYLNIDGIPIKITDTAGIRETEDTVEKIGVERSRE
HHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCHHHHHH
KIEEADLIILILDSSRELEEEDKEIINTIKDKNHIVLLNKTDLDRRIADIDLDNQINISA
HHHHCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCCCCEEEEEE
KTGYGIEELKNKIKELFFSGDINSESLIVSNVRHKQALYRSLENCEIALDRVNANEFLDL
CCCCCHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHH
ISIYVTSAMKALGEITGDELEEDVLNKIFSEFCVGK
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MREFDTICGIATPIGEGGVSIIRISGSKALDIISKIFVGKNNIDLKQMKTYTMRYGHIIE
CCCHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEE
LESKDVIDEVIISYMKGPHSYTTEDIIEINCHGGVISTNSVMNQVIKAGVRVAEPGEFTK
ECCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHCCCEECCCCHHHH
RAFLNGRIDLSQAEAVIDIIKAKTDLSMKSALMQSGGALSKQIKEIRQYLLNTLALIEYG
HHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
VDFTEDDEDIDDTLVLRVKDGIKTTILKVKELLKGADEGKIIRDGLNVVIIGKPNVGKSS
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCCHHH
LLNVLLKEKRAIVTDVPGTTRDIIEEYLNIDGIPIKITDTAGIRETEDTVEKIGVERSRE
HHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCHHHHHH
KIEEADLIILILDSSRELEEEDKEIINTIKDKNHIVLLNKTDLDRRIADIDLDNQINISA
HHHHCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCCCCEEEEEE
KTGYGIEELKNKIKELFFSGDINSESLIVSNVRHKQALYRSLENCEIALDRVNANEFLDL
CCCCCHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHH
ISIYVTSAMKALGEITGDELEEDVLNKIFSEFCVGK
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA