| Definition | Clostridium botulinum B str. Eklund 17B, complete genome. |
|---|---|
| Accession | NC_010674 |
| Length | 3,800,327 |
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The map label for this gene is murC [H]
Identifier: 187933070
GI number: 187933070
Start: 162000
End: 163382
Strand: Reverse
Name: murC [H]
Synonym: CLL_A0145
Alternate gene names: 187933070
Gene position: 163382-162000 (Counterclockwise)
Preceding gene: 187934300
Following gene: 187934649
Centisome position: 4.3
GC content: 29.36
Gene sequence:
>1383_bases TTGTCTTTCGATTTTATAAAAGATAGAGATAAGAAAGTTCACTTTATTGGTATCGGTGGAATCAGTATGAGTGGTCTTGC TGCAGTTTTATTAAATAGTGGCTATAAAGTTTCAGGTTCAGATTTTAAAGAATCAGAAATACTAAAAAAACTAAGACTTT CTGGAGCTGATATTTACATAGGTCACAGTGAAAAAAACATAAAAGATGTTGATTTAGTAGTTTATACAGCTGCTATACCT GAAAATAATCCTGAACTTATTTATGCTAAAGAAAATAATATAGAGTTAATGAATAGAGCAGAATTTTTAGGAAGTATCAT GAAGGGGCATAAATATAATGTTGCTATCTCTGGTGCACATGGAAAAACAACTTGTACTTCTATGCTATCTAATATAACTT TAAAGGCTAATTTAGATCCAACTATACTTGTTGGTGGTGAATTAGATATTATTGGTGGTAATTTCAGAATAGGTAGTAGT GATTACTTTATAACAGAAGCTTGTGAATATAAACGTTCATTTTTAAGTTTCTTCCCTTATGTTGGAGTAATTTTAAATAT AGATGCTGATCACTTAGACTACTATAAAGATATAGATGAAATTACTGAAACATTTGGACAATTTGCAGATTTAATTCCTA ATGATGGTTATTTAATAGGCTATGTTGGTGACTCTAGAGTAAAAGAAATTTTATTTAAGGCTAAATGTAATACTTTAAGT TATGGATTTGAAAATGCTGATGTAACTGCTAGAAATATAACATTCAATGAAAAGGGCTGTGCTTCTTTTGATGTTTATAA ACATAATGATAAATTATTTGATTTAACTTTAAGTAATCCTGGAGAACACAATATATTAAATGCACTTTCTTCAATTTGTG TATCACTTATATTTGATGTTAATTATGATGATATAATATGTGGATTATCAGAATGTAAGGGTGCACATAAGAGATTTGAA TATAAGGGAGAAGTAAATGGTGTTACTGTTATAGATGATTATGCTCATCATCCAGTAGAAATAAAGGCAACTTTAAATAC ATCAAAAAAAATTCCTCATAATAAAACTTTCTGTGTATTCCAACCACATACTTATACAAGAACTAAAACACTATTCGATG AATTCACAGATGCATTTTTTGATGCTGATGAAGTTGTTTTAATGGATATTTATGCTGCTCGTGAAAAAGATACTGGATTA GTTTCTTCAAACGATTTAGGCGTAGCATTAAGAGCCAAAGGCGTTAAATGCACAAATGTTCATTCTCATGATGAAGCATT AGAATATTTAAAAAATTCTGCTAAACCTAATGACTTACTTCTTACAGTAGGTGCTGGAGATGTTGTCATAGTTGGTGAAA AATACCTTAATCAAGGGAAATAA
Upstream 100 bases:
>100_bases TGGAAAAATGTATATAATTATAAATAAGTATCCAAATATCTTTATAAAGTAATTTACTAGTATTGTTTAGGATATAATTA AATATAGAGGAGTTGATATT
Downstream 100 bases:
>100_bases TAATATTATAAATAAACAGCAAAAAAATGATTGAAGTATAGTAAATGCTTCAATCATTTTTAATTAGTACTTTAAATTAC TGTAAGTTCTTTTGTTACTG
Product: UDP-N-acetylmuramate--L-alanine ligase
Products: NA
Alternate protein names: UDP-N-acetylmuramoyl-L-alanine synthetase [H]
Number of amino acids: Translated: 460; Mature: 459
Protein sequence:
>460_residues MSFDFIKDRDKKVHFIGIGGISMSGLAAVLLNSGYKVSGSDFKESEILKKLRLSGADIYIGHSEKNIKDVDLVVYTAAIP ENNPELIYAKENNIELMNRAEFLGSIMKGHKYNVAISGAHGKTTCTSMLSNITLKANLDPTILVGGELDIIGGNFRIGSS DYFITEACEYKRSFLSFFPYVGVILNIDADHLDYYKDIDEITETFGQFADLIPNDGYLIGYVGDSRVKEILFKAKCNTLS YGFENADVTARNITFNEKGCASFDVYKHNDKLFDLTLSNPGEHNILNALSSICVSLIFDVNYDDIICGLSECKGAHKRFE YKGEVNGVTVIDDYAHHPVEIKATLNTSKKIPHNKTFCVFQPHTYTRTKTLFDEFTDAFFDADEVVLMDIYAAREKDTGL VSSNDLGVALRAKGVKCTNVHSHDEALEYLKNSAKPNDLLLTVGAGDVVIVGEKYLNQGK
Sequences:
>Translated_460_residues MSFDFIKDRDKKVHFIGIGGISMSGLAAVLLNSGYKVSGSDFKESEILKKLRLSGADIYIGHSEKNIKDVDLVVYTAAIP ENNPELIYAKENNIELMNRAEFLGSIMKGHKYNVAISGAHGKTTCTSMLSNITLKANLDPTILVGGELDIIGGNFRIGSS DYFITEACEYKRSFLSFFPYVGVILNIDADHLDYYKDIDEITETFGQFADLIPNDGYLIGYVGDSRVKEILFKAKCNTLS YGFENADVTARNITFNEKGCASFDVYKHNDKLFDLTLSNPGEHNILNALSSICVSLIFDVNYDDIICGLSECKGAHKRFE YKGEVNGVTVIDDYAHHPVEIKATLNTSKKIPHNKTFCVFQPHTYTRTKTLFDEFTDAFFDADEVVLMDIYAAREKDTGL VSSNDLGVALRAKGVKCTNVHSHDEALEYLKNSAKPNDLLLTVGAGDVVIVGEKYLNQGK >Mature_459_residues SFDFIKDRDKKVHFIGIGGISMSGLAAVLLNSGYKVSGSDFKESEILKKLRLSGADIYIGHSEKNIKDVDLVVYTAAIPE NNPELIYAKENNIELMNRAEFLGSIMKGHKYNVAISGAHGKTTCTSMLSNITLKANLDPTILVGGELDIIGGNFRIGSSD YFITEACEYKRSFLSFFPYVGVILNIDADHLDYYKDIDEITETFGQFADLIPNDGYLIGYVGDSRVKEILFKAKCNTLSY GFENADVTARNITFNEKGCASFDVYKHNDKLFDLTLSNPGEHNILNALSSICVSLIFDVNYDDIICGLSECKGAHKRFEY KGEVNGVTVIDDYAHHPVEIKATLNTSKKIPHNKTFCVFQPHTYTRTKTLFDEFTDAFFDADEVVLMDIYAAREKDTGLV SSNDLGVALRAKGVKCTNVHSHDEALEYLKNSAKPNDLLLTVGAGDVVIVGEKYLNQGK
Specific function: Cell wall formation [H]
COG id: COG0773
COG function: function code M; UDP-N-acetylmuramate-alanine ligase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the MurCDEF family [H]
Homologues:
Organism=Escherichia coli, GI1786279, Length=455, Percent_Identity=35.3846153846154, Blast_Score=242, Evalue=4e-65, Organism=Escherichia coli, GI1790680, Length=397, Percent_Identity=28.9672544080605, Blast_Score=159, Evalue=3e-40,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004101 - InterPro: IPR013221 - InterPro: IPR000713 - InterPro: IPR016040 - InterPro: IPR005758 [H]
Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]
EC number: =6.3.2.8 [H]
Molecular weight: Translated: 50969; Mature: 50838
Theoretical pI: Translated: 5.32; Mature: 5.32
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.0 %Cys (Translated Protein) 1.3 %Met (Translated Protein) 3.3 %Cys+Met (Translated Protein) 2.0 %Cys (Mature Protein) 1.1 %Met (Mature Protein) 3.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSFDFIKDRDKKVHFIGIGGISMSGLAAVLLNSGYKVSGSDFKESEILKKLRLSGADIYI CCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCEECCCCCCHHHHHHHHHCCCCEEEE GHSEKNIKDVDLVVYTAAIPENNPELIYAKENNIELMNRAEFLGSIMKGHKYNVAISGAH ECCCCCCCEEEEEEEEEECCCCCCCEEEEECCCCEEHHHHHHHHHHHCCCEEEEEEECCC GKTTCTSMLSNITLKANLDPTILVGGELDIIGGNFRIGSSDYFITEACEYKRSFLSFFPY CCHHHHHHHHCEEEEECCCCEEEECCEEEEECCEEEECCCCEEEHHHHHHHHHHHHHHCC VGVILNIDADHLDYYKDIDEITETFGQFADLIPNDGYLIGYVGDSRVKEILFKAKCNTLS EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCEEE YGFENADVTARNITFNEKGCASFDVYKHNDKLFDLTLSNPGEHNILNALSSICVSLIFDV CCCCCCCEEEEEEEECCCCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHEECC NYDDIICGLSECKGAHKRFEYKGEVNGVTVIDDYAHHPVEIKATLNTSKKIPHNKTFCVF CCHHHEEEHHHCCCHHHCEEECCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCEEEEE QPHTYTRTKTLFDEFTDAFFDADEVVLMDIYAAREKDTGLVSSNDLGVALRAKGVKCTNV CCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHCCCCCCCEECCCCEEEEEECCEEEECC HSHDEALEYLKNSAKPNDLLLTVGAGDVVIVGEKYLNQGK CCHHHHHHHHHHCCCCCEEEEEECCCCEEEECHHHHCCCC >Mature Secondary Structure SFDFIKDRDKKVHFIGIGGISMSGLAAVLLNSGYKVSGSDFKESEILKKLRLSGADIYI CCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCEECCCCCCHHHHHHHHHCCCCEEEE GHSEKNIKDVDLVVYTAAIPENNPELIYAKENNIELMNRAEFLGSIMKGHKYNVAISGAH ECCCCCCCEEEEEEEEEECCCCCCCEEEEECCCCEEHHHHHHHHHHHCCCEEEEEEECCC GKTTCTSMLSNITLKANLDPTILVGGELDIIGGNFRIGSSDYFITEACEYKRSFLSFFPY CCHHHHHHHHCEEEEECCCCEEEECCEEEEECCEEEECCCCEEEHHHHHHHHHHHHHHCC VGVILNIDADHLDYYKDIDEITETFGQFADLIPNDGYLIGYVGDSRVKEILFKAKCNTLS EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCEEE YGFENADVTARNITFNEKGCASFDVYKHNDKLFDLTLSNPGEHNILNALSSICVSLIFDV CCCCCCCEEEEEEEECCCCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHEECC NYDDIICGLSECKGAHKRFEYKGEVNGVTVIDDYAHHPVEIKATLNTSKKIPHNKTFCVF CCHHHEEEHHHCCCHHHCEEECCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCEEEEE QPHTYTRTKTLFDEFTDAFFDADEVVLMDIYAAREKDTGLVSSNDLGVALRAKGVKCTNV CCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHCCCCCCCEECCCCEEEEEECCEEEECC HSHDEALEYLKNSAKPNDLLLTVGAGDVVIVGEKYLNQGK CCHHHHHHHHHHCCCCCEEEEEECCCCEEEECHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA