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The map label for this gene is dacB [H]

Identifier: 187932735

GI number: 187932735

Start: 585356

End: 586687

Strand: Reverse

Name: dacB [H]

Synonym: CLL_A0587

Alternate gene names: 187932735

Gene position: 586687-585356 (Counterclockwise)

Preceding gene: 187935362

Following gene: 187932499

Centisome position: 15.44

GC content: 26.65

Gene sequence:

>1332_bases
ATGGAGAATGTTATGAAATCACATTTAAAAACACTTATTTTTTTCCTATTTATATTTCTTGTTTCATCCATTAATGTAAT
GGCAGTGTCATCCCCTCCTGAGATAAATGCAGAAGGTTCTGTTTTAATGGATGCATCAACTGGAGAAATATTATTTAGTA
AAAATCCCGATAAAGTTTTGGAGCCGGCCTCAACGACTAAGGTAATGACTGCTATATTAACTTTAGAAAATTGTGATTTA
AATGAAGAAGTTGTAGTTACTGAAGATTTTACAAAGATTGATGGTACAACTATAGGTCTTTTAAAAGGTGATATTGTTAC
TGTAAAAGATTTATTACTTGGGCTTCTATTAGAATCTGGGAATGATGCAGCTAACGCTTTAGCTTGCCATGTTTCTTCTT
CTATTGATAAATTTGCAGTGCTTATGAATAAAAAAGCTACTGAAATTGGAGCCGTTAATACCCATTTTAAAAATCCAAGT
GGATTACCTGATCCAGAGCATTTAACTACTGCTCATGATTTAGCCCTAATTATGAATGAAGCTATTAAAAATGATAAATT
CTTAGAATTATCTAAAGTTATGACATATGAAATACCAATAAAAAATGATTCATCTAGAACAATATGGATTAATAATAAAA
ATCATATGATAAATAAGAATTCAATGTATTATTATCAATATACCCTTACAGGTAAAAATGGCTATACAACTAAATCAAAT
CATACATATACAGTAGCTGCAGAAAACAATGGGCATATTTTAGTAGCATCGTATCTTAATGCTTTAGATAAAGATAAAAA
TTTTGAAGATATGAGAACTGTTTTTAATTATGGATTTAATAATTTTTATTTTTCTAATATCTATAAAAAAGGACAGGAAA
TAGATTCTATTAAGTTACATAATGGTCTAACAGTTCCTCTTATTGCTTCAAAAGATGTAAATTGCGTTGTTCCAAATGAA
GATAAAGACAAACTTAATACTTATATTAAATTTGAAAATAAAAATCTAAATAGATTATCTTTTAATGAAGGCGATGTTAT
ATTAAAAGGTACTGTATATTTAAACGATAAAGAATTATGTAAATTAGATTTATGTGCAGGTATTTCTAGAAATTATTCTA
TAAAAGAAGATATTTATTCTTTTATAGAGAAGAATAAAAGGAATTTGATTATAGGGGCTATTATTTCTGGAGTTACATTG
CTTATTATATTAACATTAGCAATAGAATATAATAAGAAAAAGAATCGAAAAAAAAGACTATTAGAAATTACAACTTCTAT
ACCAAAAATTCAAGATGATCAATTGAAAAAAAGAAGAAGAAGACAGGATTAA

Upstream 100 bases:

>100_bases
TACATAATAATGCAATTTTATTATCAAAATTCATATTTTTATAGCATAATATATTTTTCTTTGGTAAAATAAAACTGTTG
TAATAAAAAATTTTAATATT

Downstream 100 bases:

>100_bases
ATCCTATCTTCTTCTATTTTATTTTTTCTAATAATTCTTTCATATCATCTGGCAATTCAGCTCTTAATGAAAGTTCTTTT
AAAGACCTTGGTGAAGCAAA

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MENVMKSHLKTLIFFLFIFLVSSINVMAVSSPPEINAEGSVLMDASTGEILFSKNPDKVLEPASTTKVMTAILTLENCDL
NEEVVVTEDFTKIDGTTIGLLKGDIVTVKDLLLGLLLESGNDAANALACHVSSSIDKFAVLMNKKATEIGAVNTHFKNPS
GLPDPEHLTTAHDLALIMNEAIKNDKFLELSKVMTYEIPIKNDSSRTIWINNKNHMINKNSMYYYQYTLTGKNGYTTKSN
HTYTVAAENNGHILVASYLNALDKDKNFEDMRTVFNYGFNNFYFSNIYKKGQEIDSIKLHNGLTVPLIASKDVNCVVPNE
DKDKLNTYIKFENKNLNRLSFNEGDVILKGTVYLNDKELCKLDLCAGISRNYSIKEDIYSFIEKNKRNLIIGAIISGVTL
LIILTLAIEYNKKKNRKKRLLEITTSIPKIQDDQLKKRRRRQD

Sequences:

>Translated_443_residues
MENVMKSHLKTLIFFLFIFLVSSINVMAVSSPPEINAEGSVLMDASTGEILFSKNPDKVLEPASTTKVMTAILTLENCDL
NEEVVVTEDFTKIDGTTIGLLKGDIVTVKDLLLGLLLESGNDAANALACHVSSSIDKFAVLMNKKATEIGAVNTHFKNPS
GLPDPEHLTTAHDLALIMNEAIKNDKFLELSKVMTYEIPIKNDSSRTIWINNKNHMINKNSMYYYQYTLTGKNGYTTKSN
HTYTVAAENNGHILVASYLNALDKDKNFEDMRTVFNYGFNNFYFSNIYKKGQEIDSIKLHNGLTVPLIASKDVNCVVPNE
DKDKLNTYIKFENKNLNRLSFNEGDVILKGTVYLNDKELCKLDLCAGISRNYSIKEDIYSFIEKNKRNLIIGAIISGVTL
LIILTLAIEYNKKKNRKKRLLEITTSIPKIQDDQLKKRRRRQD
>Mature_443_residues
MENVMKSHLKTLIFFLFIFLVSSINVMAVSSPPEINAEGSVLMDASTGEILFSKNPDKVLEPASTTKVMTAILTLENCDL
NEEVVVTEDFTKIDGTTIGLLKGDIVTVKDLLLGLLLESGNDAANALACHVSSSIDKFAVLMNKKATEIGAVNTHFKNPS
GLPDPEHLTTAHDLALIMNEAIKNDKFLELSKVMTYEIPIKNDSSRTIWINNKNHMINKNSMYYYQYTLTGKNGYTTKSN
HTYTVAAENNGHILVASYLNALDKDKNFEDMRTVFNYGFNNFYFSNIYKKGQEIDSIKLHNGLTVPLIASKDVNCVVPNE
DKDKLNTYIKFENKNLNRLSFNEGDVILKGTVYLNDKELCKLDLCAGISRNYSIKEDIYSFIEKNKRNLIIGAIISGVTL
LIILTLAIEYNKKKNRKKRLLEITTSIPKIQDDQLKKRRRRQD

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=283, Percent_Identity=29.6819787985866, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87082022, Length=305, Percent_Identity=28.1967213114754, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786851, Length=315, Percent_Identity=27.6190476190476, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87082054, Length=262, Percent_Identity=27.0992366412214, Blast_Score=79, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 49919; Mature: 49919

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENVMKSHLKTLIFFLFIFLVSSINVMAVSSPPEINAEGSVLMDASTGEILFSKNPDKVL
CCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEECCCCCEEEECCCHHHC
EPASTTKVMTAILTLENCDLNEEVVVTEDFTKIDGTTIGLLKGDIVTVKDLLLGLLLESG
CCCHHHHHHHHEEEEECCCCCCCEEEEECCEECCCCEEEEEECCCHHHHHHHHHHHHHCC
NDAANALACHVSSSIDKFAVLMNKKATEIGAVNTHFKNPSGLPDPEHLTTAHDLALIMNE
CCHHHHEEEEHHCCHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AIKNDKFLELSKVMTYEIPIKNDSSRTIWINNKNHMINKNSMYYYQYTLTGKNGYTTKSN
HHCCCCEEEEEEEEEEEEEECCCCCCEEEECCCCCEECCCCEEEEEEEEECCCCCCCCCC
HTYTVAAENNGHILVASYLNALDKDKNFEDMRTVFNYGFNNFYFSNIYKKGQEIDSIKLH
CEEEEEECCCCCEEEEHHHHHHCCCCCHHHHHHHHHCCCCCEEHHHHHHCCCCCCEEEEC
NGLTVPLIASKDVNCVVPNEDKDKLNTYIKFENKNLNRLSFNEGDVILKGTVYLNDKELC
CCCEEEEEECCCCEEECCCCCCCCCEEEEEECCCCCCEEECCCCCEEEEEEEEECCHHHE
KLDLCAGISRNYSIKEDIYSFIEKNKRNLIIGAIISGVTLLIILTLAIEYNKKKNRKKRL
EEHHHCCCCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LEITTSIPKIQDDQLKKRRRRQD
HHHHHCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MENVMKSHLKTLIFFLFIFLVSSINVMAVSSPPEINAEGSVLMDASTGEILFSKNPDKVL
CCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEECCCCCEEEECCCHHHC
EPASTTKVMTAILTLENCDLNEEVVVTEDFTKIDGTTIGLLKGDIVTVKDLLLGLLLESG
CCCHHHHHHHHEEEEECCCCCCCEEEEECCEECCCCEEEEEECCCHHHHHHHHHHHHHCC
NDAANALACHVSSSIDKFAVLMNKKATEIGAVNTHFKNPSGLPDPEHLTTAHDLALIMNE
CCHHHHEEEEHHCCHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AIKNDKFLELSKVMTYEIPIKNDSSRTIWINNKNHMINKNSMYYYQYTLTGKNGYTTKSN
HHCCCCEEEEEEEEEEEEEECCCCCCEEEECCCCCEECCCCEEEEEEEEECCCCCCCCCC
HTYTVAAENNGHILVASYLNALDKDKNFEDMRTVFNYGFNNFYFSNIYKKGQEIDSIKLH
CEEEEEECCCCCEEEEHHHHHHCCCCCHHHHHHHHHCCCCCEEHHHHHHCCCCCCEEEEC
NGLTVPLIASKDVNCVVPNEDKDKLNTYIKFENKNLNRLSFNEGDVILKGTVYLNDKELC
CCCEEEEEECCCCEEECCCCCCCCCEEEEEECCCCCCEEECCCCCEEEEEEEEECCHHHE
KLDLCAGISRNYSIKEDIYSFIEKNKRNLIIGAIISGVTLLIILTLAIEYNKKKNRKKRL
EEHHHCCCCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LEITTSIPKIQDDQLKKRRRRQD
HHHHHCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]