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The map label for this gene is pyk [H]

Identifier: 187932499

GI number: 187932499

Start: 556394

End: 557812

Strand: Reverse

Name: pyk [H]

Synonym: CLL_A0557

Alternate gene names: 187932499

Gene position: 557812-556394 (Counterclockwise)

Preceding gene: 187932735

Following gene: 187934019

Centisome position: 14.68

GC content: 33.12

Gene sequence:

>1419_bases
ATGCAAAAAACTAAAATGATTTTTACTATTGGACCTGCAAGTGATAATGAGGAGATCATAAGGAAATTTATAGGAATTGG
TATGAATGCGGCAAGATTAAATTTTTCGCATGGTACTTATGAATCACACAGAGAGAAGATAGAATTAATAAAAAAAGTAA
GAGAAGATATGGGAACTCCGACTGCGATAGTCTTAGACATAAAAGGACCTAAGATAAGAACACATAACTTTGTAAATGAT
GGGGTAGAATTAAAAACTGGTCAAGAATTCTGTTTCGTATGCGGAGATGAACTTCTTGGTGATGAAGAAAGATGTTCTAT
TACTTATGAAGAGCTTTATAAAGACATACAAGTGGGTGGAGAAATTCTTGTTGATGATGGTCTTTTAAGATTTAAAGTTA
TAGCTGTTGAAGGCAAATCTATTAGATGTGCTGTAACAGTTGGTGGAATGATTAAAAATCACAAAGGTGTTAACGTTCCC
AACGTAATAATAAGACTACCTTCAGTAACAGAAAAAGATATAGCTGATATAAAATTTGGTTGTGAAATGGGTGTAGACTT
TATAGCAGCATCTTTCATAAGAAAAGCTAGCGATGTAGCTGATGTAAAGAAGATACTTTCTGAAAATAATGGTAAACATA
TTAAAGTAATAGCTAAAATTGAAAATCAAGAAGGCGTAGATAACATAGATTCTATAATAGAACAAACTGACGCTGTAATG
GTTGCTAGAGGAGATATGGGAGTTGAAATTCCTATTCAAAAAGTTCCTATAATTCAAAAAATGATAATTAGAAAATGTAA
TGATTCAGGAAAATTCGTTGTAACAGCAACTCAAATGCTTGATTCAATGATTAGAAACTCTTTACCAACTAGAGCAGAGG
CAAGTGATATTTGCAATGCTATATTTGATGGAACTGATGCTATAATGTTAAGTGGTGAAAGTGCCTCTGGAAATTTCCCA
ATTGAGGCAGCTACAACAATGTCTGAGATTGCGCAAGAAGCAGAAGCTCATTTAGATTATGCTCACTTAACAGCAAGGTT
AAGAGAACCCTCTCTTACTGATTATTCTGGTGCAATAAGTTATACAGCTTGTAGAACTTCAAATTTATTAAATGCAGCTG
CAATAGTAGCTGCTACTAAAAGTGGTGCTACAGCTACTATGATTTCAAGATATAGACCAAAATCTCCTGTTATAGCAATA
ACTCCTTATGAACAAGTAAGAAGAAGTTTAGCTTTAACTTTTGGTGTATTACCAATGCTTTGCGAAATGTATAATACTAC
TGATGAAATCTTAGTGGAAGCTAGAAGATCAGTGTTTGAATTAAATTTTGCTTTACCTGGTGATGATATTGTAGTTGCTG
CTGGAATGCCAACAACTCAAACTGGTGGAACAAATATGTTAAAAATAGAGAAATTATAA

Upstream 100 bases:

>100_bases
AAAACTAAATTAATATTATAGAATTTAGTTAATTTTATTATTAGGCTTTAATATTACATAAATATAAAAAGGCATTAAAA
TTTATAGGAGAGGTGTTTAT

Downstream 100 bases:

>100_bases
TATTTATTTTTAAATTTAAAATACTTTAAAACTAAAATAAGCTGTTTCAACATACAATATCCCTTGTATTTGAAACAGCT
TATTTTAATCTATGTATTAA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MQKTKMIFTIGPASDNEEIIRKFIGIGMNAARLNFSHGTYESHREKIELIKKVREDMGTPTAIVLDIKGPKIRTHNFVND
GVELKTGQEFCFVCGDELLGDEERCSITYEELYKDIQVGGEILVDDGLLRFKVIAVEGKSIRCAVTVGGMIKNHKGVNVP
NVIIRLPSVTEKDIADIKFGCEMGVDFIAASFIRKASDVADVKKILSENNGKHIKVIAKIENQEGVDNIDSIIEQTDAVM
VARGDMGVEIPIQKVPIIQKMIIRKCNDSGKFVVTATQMLDSMIRNSLPTRAEASDICNAIFDGTDAIMLSGESASGNFP
IEAATTMSEIAQEAEAHLDYAHLTARLREPSLTDYSGAISYTACRTSNLLNAAAIVAATKSGATATMISRYRPKSPVIAI
TPYEQVRRSLALTFGVLPMLCEMYNTTDEILVEARRSVFELNFALPGDDIVVAAGMPTTQTGGTNMLKIEKL

Sequences:

>Translated_472_residues
MQKTKMIFTIGPASDNEEIIRKFIGIGMNAARLNFSHGTYESHREKIELIKKVREDMGTPTAIVLDIKGPKIRTHNFVND
GVELKTGQEFCFVCGDELLGDEERCSITYEELYKDIQVGGEILVDDGLLRFKVIAVEGKSIRCAVTVGGMIKNHKGVNVP
NVIIRLPSVTEKDIADIKFGCEMGVDFIAASFIRKASDVADVKKILSENNGKHIKVIAKIENQEGVDNIDSIIEQTDAVM
VARGDMGVEIPIQKVPIIQKMIIRKCNDSGKFVVTATQMLDSMIRNSLPTRAEASDICNAIFDGTDAIMLSGESASGNFP
IEAATTMSEIAQEAEAHLDYAHLTARLREPSLTDYSGAISYTACRTSNLLNAAAIVAATKSGATATMISRYRPKSPVIAI
TPYEQVRRSLALTFGVLPMLCEMYNTTDEILVEARRSVFELNFALPGDDIVVAAGMPTTQTGGTNMLKIEKL
>Mature_472_residues
MQKTKMIFTIGPASDNEEIIRKFIGIGMNAARLNFSHGTYESHREKIELIKKVREDMGTPTAIVLDIKGPKIRTHNFVND
GVELKTGQEFCFVCGDELLGDEERCSITYEELYKDIQVGGEILVDDGLLRFKVIAVEGKSIRCAVTVGGMIKNHKGVNVP
NVIIRLPSVTEKDIADIKFGCEMGVDFIAASFIRKASDVADVKKILSENNGKHIKVIAKIENQEGVDNIDSIIEQTDAVM
VARGDMGVEIPIQKVPIIQKMIIRKCNDSGKFVVTATQMLDSMIRNSLPTRAEASDICNAIFDGTDAIMLSGESASGNFP
IEAATTMSEIAQEAEAHLDYAHLTARLREPSLTDYSGAISYTACRTSNLLNAAAIVAATKSGATATMISRYRPKSPVIAI
TPYEQVRRSLALTFGVLPMLCEMYNTTDEILVEARRSVFELNFALPGDDIVVAAGMPTTQTGGTNMLKIEKL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=42.7402862985685, Blast_Score=360, Evalue=2e-99,
Organism=Homo sapiens, GI33286422, Length=489, Percent_Identity=42.5357873210634, Blast_Score=356, Evalue=3e-98,
Organism=Homo sapiens, GI33286420, Length=489, Percent_Identity=42.5357873210634, Blast_Score=356, Evalue=3e-98,
Organism=Homo sapiens, GI10835121, Length=495, Percent_Identity=41.010101010101, Blast_Score=339, Evalue=4e-93,
Organism=Homo sapiens, GI32967597, Length=495, Percent_Identity=41.010101010101, Blast_Score=338, Evalue=5e-93,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=45.4861111111111, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=45.4861111111111, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=44.0677966101695, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=44.0677966101695, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI310128738, Length=211, Percent_Identity=44.5497630331754, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1787965, Length=477, Percent_Identity=43.8155136268344, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=36.2683438155136, Blast_Score=287, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI17544584, Length=484, Percent_Identity=42.7685950413223, Blast_Score=352, Evalue=3e-97,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=40.2892561983471, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=40.2892561983471, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI71984406, Length=484, Percent_Identity=40.2892561983471, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=40.2892561983471, Blast_Score=300, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6319279, Length=455, Percent_Identity=41.5384615384615, Blast_Score=318, Evalue=8e-88,
Organism=Saccharomyces cerevisiae, GI6324923, Length=482, Percent_Identity=39.8340248962656, Blast_Score=309, Evalue=6e-85,
Organism=Drosophila melanogaster, GI24648964, Length=482, Percent_Identity=41.2863070539419, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI28571814, Length=482, Percent_Identity=41.2863070539419, Blast_Score=342, Evalue=5e-94,
Organism=Drosophila melanogaster, GI24648966, Length=410, Percent_Identity=41.9512195121951, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI24581235, Length=433, Percent_Identity=36.4896073903002, Blast_Score=268, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24646914, Length=289, Percent_Identity=38.4083044982699, Blast_Score=194, Evalue=8e-50,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51565; Mature: 51565

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKTKMIFTIGPASDNEEIIRKFIGIGMNAARLNFSHGTYESHREKIELIKKVREDMGTP
CCCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCC
TAIVLDIKGPKIRTHNFVNDGVELKTGQEFCFVCGDELLGDEERCSITYEELYKDIQVGG
EEEEEECCCCEEEECCCCCCCEEEECCCCEEEEECHHHCCCCHHCEEEHHHHHHHHHCCC
EILVDDGLLRFKVIAVEGKSIRCAVTVGGMIKNHKGVNVPNVIIRLPSVTEKDIADIKFG
EEEEECCCEEEEEEEEECCEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHH
CEMGVDFIAASFIRKASDVADVKKILSENNGKHIKVIAKIENQEGVDNIDSIIEQTDAVM
HHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHCCEEE
VARGDMGVEIPIQKVPIIQKMIIRKCNDSGKFVVTATQMLDSMIRNSLPTRAEASDICNA
EEECCCCEECCHHHCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCCCCCHHHHHHH
IFDGTDAIMLSGESASGNFPIEAATTMSEIAQEAEAHLDYAHLTARLREPSLTDYSGAIS
HHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEE
YTACRTSNLLNAAAIVAATKSGATATMISRYRPKSPVIAITPYEQVRRSLALTFGVLPML
EEEHHCCCCHHHEEEEEECCCCCHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHH
CEMYNTTDEILVEARRSVFELNFALPGDDIVVAAGMPTTQTGGTNMLKIEKL
HHHHCCHHHHHHHHHHHHEEEEEECCCCCEEEECCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MQKTKMIFTIGPASDNEEIIRKFIGIGMNAARLNFSHGTYESHREKIELIKKVREDMGTP
CCCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCC
TAIVLDIKGPKIRTHNFVNDGVELKTGQEFCFVCGDELLGDEERCSITYEELYKDIQVGG
EEEEEECCCCEEEECCCCCCCEEEECCCCEEEEECHHHCCCCHHCEEEHHHHHHHHHCCC
EILVDDGLLRFKVIAVEGKSIRCAVTVGGMIKNHKGVNVPNVIIRLPSVTEKDIADIKFG
EEEEECCCEEEEEEEEECCEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHH
CEMGVDFIAASFIRKASDVADVKKILSENNGKHIKVIAKIENQEGVDNIDSIIEQTDAVM
HHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHCCEEE
VARGDMGVEIPIQKVPIIQKMIIRKCNDSGKFVVTATQMLDSMIRNSLPTRAEASDICNA
EEECCCCEECCHHHCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCCCCCHHHHHHH
IFDGTDAIMLSGESASGNFPIEAATTMSEIAQEAEAHLDYAHLTARLREPSLTDYSGAIS
HHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEE
YTACRTSNLLNAAAIVAATKSGATATMISRYRPKSPVIAITPYEQVRRSLALTFGVLPML
EEEHHCCCCHHHEEEEEECCCCCHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHH
CEMYNTTDEILVEARRSVFELNFALPGDDIVVAAGMPTTQTGGTNMLKIEKL
HHHHCCHHHHHHHHHHHHEEEEEECCCCCEEEECCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842; 7559358 [H]