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The map label for this gene is ynfL [H]

Identifier: 187930679

GI number: 187930679

Start: 3785538

End: 3786422

Strand: Reverse

Name: ynfL [H]

Synonym: Rpic_3615

Alternate gene names: 187930679

Gene position: 3786422-3785538 (Counterclockwise)

Preceding gene: 187930684

Following gene: 187930678

Centisome position: 96.04

GC content: 68.93

Gene sequence:

>885_bases
ATGCGAGAGATCAGCCTGGACCGCCTGCGCACCCTCGTCGAAGTCGCCGGCCGCGGATCGTTTGCCGACGCGGCGCGGGC
CCTGCATCTGGCAGCGCCCACCGTGAGCCTGCATATCGCCGAGCTGGAAGATCGCGTGGGCGCGCAACTTCTGTCACGCA
AGCGCGGGCAGGTGCAGCCCACCGCCATTGGCGAGGTGATGGTGGAGCGCGCGCGCAAGCTGCTCGCCGATGCCGAGCAG
GCGCTGGACGACATCCAACGCCAGGTGCAGGGACTGAGCGGGCGCGTACGGCTGGGCGCCTCCACCGGCGCCATCGCGCA
TCTGCTGCCGCAGGCGCTGGAGGTGCTCCGGCAAGACCACCCTGGCATCGACGTGCAGGTGGCAGTGCTCACTTCGCAGG
AAACGCTGGCGGGTCTGGCCGATGGCGCGCTCGACATTGGCCTTGTGGCGCTGCCGCAATCGCAGGTGGCGGGGCTGGCG
ATCAAGCCGTGGCGGCGCGATCCCGTGATGGCGTTCCTGCCTTCGCACTGGACGAGTCCGGCACGCATCACGCCCGCGTG
GCTTGCCGCGCAGCCGCTGATCCTGAACGACGCCACCACACGTCTCTCGCGCCAGACGACCGAGTGGTTCGCCACGGACG
GTTATCACCCCGTGCCGCGCATCCAGCTCAACTACAACGACGCTATCAAAAGCCTCGTTGCCGCCGGTTATGGCGCCACG
CTGCTCCCGCACGAGGCGTCCGCCGTACCGTCGGATGTGCGCATCACCATGCGGCCGCTGCGGCCGGCGTTGTGGCGTGA
ACTTGGCATCGCGCACCGGGCCGGCCACGTCGAGCGATCGACGCAGCACGTGCTGGACGTGCTTTGGATGCTGCGCGCGA
AATAG

Upstream 100 bases:

>100_bases
GGGAAAGAAGTTGGTGATGAATGCGAGCCCCCATCTTCGAGCGCGACCTAGAATATGAAAAGTTGAATTTCCCAACCCTC
ACATTCAGCTTTTCTGAATC

Downstream 100 bases:

>100_bases
CGCATCGCGCGCCTTGGCCACGTGACGAGGCGCAGAATCCCGCTGAATAAAGTTTGACTTCTTATGTCGCGACAACTATC
GTAAGATATGTTTTTCGACA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MREISLDRLRTLVEVAGRGSFADAARALHLAAPTVSLHIAELEDRVGAQLLSRKRGQVQPTAIGEVMVERARKLLADAEQ
ALDDIQRQVQGLSGRVRLGASTGAIAHLLPQALEVLRQDHPGIDVQVAVLTSQETLAGLADGALDIGLVALPQSQVAGLA
IKPWRRDPVMAFLPSHWTSPARITPAWLAAQPLILNDATTRLSRQTTEWFATDGYHPVPRIQLNYNDAIKSLVAAGYGAT
LLPHEASAVPSDVRITMRPLRPALWRELGIAHRAGHVERSTQHVLDVLWMLRAK

Sequences:

>Translated_294_residues
MREISLDRLRTLVEVAGRGSFADAARALHLAAPTVSLHIAELEDRVGAQLLSRKRGQVQPTAIGEVMVERARKLLADAEQ
ALDDIQRQVQGLSGRVRLGASTGAIAHLLPQALEVLRQDHPGIDVQVAVLTSQETLAGLADGALDIGLVALPQSQVAGLA
IKPWRRDPVMAFLPSHWTSPARITPAWLAAQPLILNDATTRLSRQTTEWFATDGYHPVPRIQLNYNDAIKSLVAAGYGAT
LLPHEASAVPSDVRITMRPLRPALWRELGIAHRAGHVERSTQHVLDVLWMLRAK
>Mature_294_residues
MREISLDRLRTLVEVAGRGSFADAARALHLAAPTVSLHIAELEDRVGAQLLSRKRGQVQPTAIGEVMVERARKLLADAEQ
ALDDIQRQVQGLSGRVRLGASTGAIAHLLPQALEVLRQDHPGIDVQVAVLTSQETLAGLADGALDIGLVALPQSQVAGLA
IKPWRRDPVMAFLPSHWTSPARITPAWLAAQPLILNDATTRLSRQTTEWFATDGYHPVPRIQLNYNDAIKSLVAAGYGAT
LLPHEASAVPSDVRITMRPLRPALWRELGIAHRAGHVERSTQHVLDVLWMLRAK

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=249, Percent_Identity=30.1204819277108, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1790399, Length=282, Percent_Identity=26.5957446808511, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788481, Length=294, Percent_Identity=26.530612244898, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788748, Length=289, Percent_Identity=28.0276816608997, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI2367136, Length=286, Percent_Identity=26.2237762237762, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788887, Length=264, Percent_Identity=27.6515151515151, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787601, Length=145, Percent_Identity=31.7241379310345, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790208, Length=246, Percent_Identity=25.609756097561, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081904, Length=148, Percent_Identity=34.4594594594595, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788297, Length=251, Percent_Identity=25.8964143426295, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790262, Length=196, Percent_Identity=27.5510204081633, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32027; Mature: 32027

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS50931 HTH_LYSR ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREISLDRLRTLVEVAGRGSFADAARALHLAAPTVSLHIAELEDRVGAQLLSRKRGQVQP
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCH
TAIGEVMVERARKLLADAEQALDDIQRQVQGLSGRVRLGASTGAIAHLLPQALEVLRQDH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCC
PGIDVQVAVLTSQETLAGLADGALDIGLVALPQSQVAGLAIKPWRRDPVMAFLPSHWTSP
CCCCEEEEEEECHHHHHHHHCCCHHCEEEECCHHHHCCEEECCCCCCCCEEECCCCCCCC
ARITPAWLAAQPLILNDATTRLSRQTTEWFATDGYHPVPRIQLNYNDAIKSLVAAGYGAT
CCCCHHHHHCCCEEECHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHCCCCE
LLPHEASAVPSDVRITMRPLRPALWRELGIAHRAGHVERSTQHVLDVLWMLRAK
ECCCCCCCCCCCCEEEEHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MREISLDRLRTLVEVAGRGSFADAARALHLAAPTVSLHIAELEDRVGAQLLSRKRGQVQP
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCH
TAIGEVMVERARKLLADAEQALDDIQRQVQGLSGRVRLGASTGAIAHLLPQALEVLRQDH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCC
PGIDVQVAVLTSQETLAGLADGALDIGLVALPQSQVAGLAIKPWRRDPVMAFLPSHWTSP
CCCCEEEEEEECHHHHHHHHCCCHHCEEEECCHHHHCCEEECCCCCCCCEEECCCCCCCC
ARITPAWLAAQPLILNDATTRLSRQTTEWFATDGYHPVPRIQLNYNDAIKSLVAAGYGAT
CCCCHHHHHCCCEEECHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHCCCCE
LLPHEASAVPSDVRITMRPLRPALWRELGIAHRAGHVERSTQHVLDVLWMLRAK
ECCCCCCCCCCCCEEEEHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]